Rita Graceffa
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View article: Building a community lightsource meta-infrastructure to accelerate battery innovation in Europe
Building a community lightsource meta-infrastructure to accelerate battery innovation in Europe Open
Breakthroughs in battery research are imperative to provide society with batteries that are safe and sustainable, have a high energy density, and have a long cycle life at low cost. Recent advances in research methodologies, the emergence …
View article: Development of crystal optics for X-ray multi-projection imaging for synchrotron and XFEL sources
Development of crystal optics for X-ray multi-projection imaging for synchrotron and XFEL sources Open
X-ray multi-projection imaging (XMPI) is an emerging experimental technique for the acquisition of rotation-free, time-resolved, volumetric information on stochastic processes. The technique is developed for high-brilliance light-source fa…
View article: Development of crystal optics for Multi-Projection X-ray Imaging for synchrotron and XFEL sources
Development of crystal optics for Multi-Projection X-ray Imaging for synchrotron and XFEL sources Open
X-ray Multi-Projection Imaging (XMPI) is an emerging technology that allows for the acquisition of millions of 3D images per second in samples opaque to visible light. This breakthrough capability enables volumetric observation of fast sto…
Megahertz X-ray multi-projection imaging Open
X-ray time-resolved tomography is one of the most popular X-ray techniques to probe dynamics in three dimensions (3D). Recent developments in time-resolved tomography opened the possibility of recording kilohertz-rate 3D movies. However, t…
Megahertz X-ray Multi-projection imaging Open
X-ray time-resolved tomography is one of the most popular X-ray techniques to probe dynamics in three dimensions (3D). Recent developments in time-resolved tomography opened the possibility of recording kilohertz-rate 3D movies. However, t…
View article: A small-angle X-ray scattering study of red blood cells in continuous flow
A small-angle X-ray scattering study of red blood cells in continuous flow Open
Owing to their large penetration depth and high resolution, X-rays are ideally suited to study structures and structural changes within intact biological cells. For this reason, X-ray-based techniques have been used to investigate adhesive…
Wall-free droplet microfluidics for probing biological processes by high-brilliance X-ray scattering techniques Open
Here we review probing biological processes initiated by the deposition of droplets on surfaces by micro- and nanobeam X-ray scattering techniques using synchrotron radiation and X-ray free-electron laser sources. We review probing droplet…
View article: Evaluation of serial crystallographic structure determination within megahertz pulse trains
Evaluation of serial crystallographic structure determination within megahertz pulse trains Open
The new European X-ray Free-Electron Laser (European XFEL) is the first X-ray free-electron laser capable of delivering intense X-ray pulses with a megahertz interpulse spacing in a wavelength range suitable for atomic resolution structure…
Megahertz x-ray microscopy at x-ray free-electron laser and synchrotron sources Open
Modern emerging technologies, such as additive manufacturing, bioprinting, and new material production, require novel metrology tools to probe fundamental high-speed dynamics happening in such systems. Here we demonstrate the application o…
Megahertz X-ray microscopy at X-ray Free-Electron Laser and Synchrotron sources Open
We demonstrate X-ray phase contrast microscopy performed at the European X-ray Free-Electron Laser sampled at 1.128 MHz rate. We have applied this method to image stochastic processes induced by an optical laser incident on water-filled ca…
The Single Particles, Clusters and Biomolecules and Serial Femtosecond Crystallography instrument of the European XFEL: initial installation Open
The European X-ray Free-Electron Laser (FEL) became the first operational high-repetition-rate hard X-ray FEL with first lasing in May 2017. Biological structure determination has already benefitted from the unique properties and capabilit…
Initial observations of the femtosecond timing jitter at the European XFEL Open
Intense, ultrashort, and high-repetition-rate X-ray pulses, combined with a femtosecond optical laser, allow pump-probe experiments with fast data acquisition and femtosecond time resolution. However, the relative timing of the X-ray pulse…
A versatile liquid-jet setup for the European XFEL Open
The SPB/SFX instrument of the European XFEL provides unique possibilities for high-throughput serial femtosecond crystallography. This publication presents the liquid-jet sample delivery setup of this instrument. The setup is compatible wi…
Supplementary document for Megahertz X-ray microscopy at X-ray Free-Electron Laser and Synchrotron sources - 4067998.pdf Open
Supplemental 1
View article: Megahertz serial crystallography
Megahertz serial crystallography Open
The new European X-ray Free-Electron Laser is the first X-ray free-electron laser capable of delivering X-ray pulses with a megahertz inter-pulse spacing, more than four orders of magnitude higher than previously possible. However, to date…
View article: Rapid sample delivery for megahertz serial crystallography at X-ray FELs
Rapid sample delivery for megahertz serial crystallography at X-ray FELs Open
Liquid microjets are a common means of delivering protein crystals to the focus of X-ray free-electron lasers (FELs) for serial femtosecond crystallography measurements. The high X-ray intensity in the focus initiates an explosion of the m…
Perspectives: DOD Inkjets at High and Ultra-Brilliant Light Sources Open
Droplets generated by inkjets can serve as carriers for biological solutions and objects including nanocrystals.X-ray scattering from ballistic droplets loaded with biological matter provides conformational and structural information in th…
MHz frame rate hard X-ray phase-contrast imaging using synchrotron radiation Open
Third generation synchrotron light sources offer high photon flux, partial spatial coherence, and ~10-10 s pulse widths. These enable hard X-ray phase-contrast imaging (XPCI) with single-bunch temporal resolutions. In this work, we exploit…
Rapid Acquisition of X‐Ray Scattering Data from Droplet‐Encapsulated Protein Systems Open
Encapsulating reacting biological or chemical samples in microfluidic droplets has the great advantage over single‐phase flows of providing separate reaction compartments. These compartments can be filled in a combinatoric way and prevent …
Following DNA Compaction During the Cell Cycle by X-ray Nanodiffraction Open
X-ray imaging of intact biological cells is emerging as a complementary method to visible light or electron microscopy. Owing to the high penetration depth and small wavelength of X-rays, it is possible to resolve subcellular structures at…
X-rays Reveal the Internal Structure of Keratin Bundles in Whole Cells Open
In recent years, X-ray imaging of biological cells has emerged as a complementary alternative to fluorescence and electron microscopy. Different techniques were established and successfully applied to macromolecular assemblies and structur…