R. Anand
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View article: Multiple human transgenes prolong survival of triple-carbohydrate knockout porcine kidney xenografts in nonhuman primates
Multiple human transgenes prolong survival of triple-carbohydrate knockout porcine kidney xenografts in nonhuman primates Open
Genetically modified pigs are being developed to address the critical shortage of human organs for transplantation. We have previously demonstrated significantly prolonged survival of porcine xenografts devoid of three major carbohydrate x…
View article: Design and testing of a humanized porcine donor for xenotransplantation
Design and testing of a humanized porcine donor for xenotransplantation Open
View article: Extensive Mammalian Germline Genome Engineering
Extensive Mammalian Germline Genome Engineering Open
Xenotransplantation, specifically the use of porcine organs for human transplantation, has long been sought after as an alternative for patients suffering from organ failure. However, clinical application of this approach has been impeded …
View article: CRISPR/Cas9 cleavages in budding yeast reveal templated insertions and strand-specific insertion/deletion profiles
CRISPR/Cas9 cleavages in budding yeast reveal templated insertions and strand-specific insertion/deletion profiles Open
Significance Using budding yeast, we address how Cas9 protein and its guide RNA (gRNA) create double-strand chromosome breaks (DSBs), and explore whether binding of Cas9::gRNA influences subsequent DSB repair by nonhomologous end-joining. …
View article: CRISPR/Cas9 cleavages in budding yeast reveal templated insertions and strand-specific insertion/deletion profiles
CRISPR/Cas9 cleavages in budding yeast reveal templated insertions and strand-specific insertion/deletion profiles Open
Harnessing CRISPR-Cas9 technology has provided an unprecedented ability to modify genomic loci via DNA double-strand break (DSB) induction and repair. We have analyzed nonhomologous end-joining (NHEJ) repair induced by Cas9 in the budding …
View article: Cas9-mediated gene editing in Saccharomyces cerevisiae
Cas9-mediated gene editing in Saccharomyces cerevisiae Open
We describe an easy, e cient, and inexpensive way to clone gRNAs into plasmid vectors carrying Cas9.The method involves designing two 25 nt complementary sequences that can be duplexed and subsequently ligated into the BplI restriction sit…
View article: Rad51-mediated double-strand break repair and mismatch correction of divergent substrates
Rad51-mediated double-strand break repair and mismatch correction of divergent substrates Open
View article: Differential requirement of Srs2 helicase and Rad51 displacement activities in replication of hairpin-forming CAG/CTG repeats
Differential requirement of Srs2 helicase and Rad51 displacement activities in replication of hairpin-forming CAG/CTG repeats Open
Trinucleotide repeats are a source of genome instability, causing replication fork stalling, chromosome fragility, and impaired repair. Specialized helicases play an important role in unwinding DNA structures to maintain genome stability. …
View article: <i>MTE1</i> Functions with <i>MPH1</i> in Double-Strand Break Repair
<i>MTE1</i> Functions with <i>MPH1</i> in Double-Strand Break Repair Open
Double-strand DNA breaks occur upon exposure of cells to ionizing radiation and certain chemical agents or indirectly through replication fork collapse at DNA damage sites. If left unrepaired, double-strand breaks can cause genome instabil…
View article: YGR042W/MTE1 Functions in Double-Strand Break Repair with MPH1
YGR042W/MTE1 Functions in Double-Strand Break Repair with MPH1 Open
Double-strand DNA breaks occur upon exposure of cells to agents such as ionizing radiation and ultraviolet light or indirectly through replication fork collapse at DNA damage sites. If left unrepaired double-strand breaks can cause genome …