Rob Haselberg
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View article: Targeting oxidized phosphatidylcholines in SOD1-associated ALS: therapeutic potential of PC-OxPL-VecTab®
Targeting oxidized phosphatidylcholines in SOD1-associated ALS: therapeutic potential of PC-OxPL-VecTab® Open
Amyotrophic lateral sclerosis (ALS) is a devastating neurodegenerative disease characterized by progressive motor neuron degeneration. Mutations in the superoxide dismutase 1 (SOD1) gene account for a significant fraction of familial ALS (…
View article: Online multimethod platform for comprehensive characterization of monoclonal antibodies in cell culture fluid from injection of crude sample - Incorporation of middle-up and bottom-up workflows
Online multimethod platform for comprehensive characterization of monoclonal antibodies in cell culture fluid from injection of crude sample - Incorporation of middle-up and bottom-up workflows Open
View article: Characterization of monoclonal antibody charge variants under near-native separation conditions using nanoflow sheath liquid capillary electrophoresis-mass spectrometry
Characterization of monoclonal antibody charge variants under near-native separation conditions using nanoflow sheath liquid capillary electrophoresis-mass spectrometry Open
This manuscript describes a new CZE-MS method that permits heterogeneity assessment of mAbs under MS-compatible conditions, providing charge variant separation.
View article: Characterization of Monoclonal Antibody Charge Variants under Near-Native Separation Conditions using Nanoflow Sheath Liquid Capillary Electrophoresis-Mass Spectrometry
Characterization of Monoclonal Antibody Charge Variants under Near-Native Separation Conditions using Nanoflow Sheath Liquid Capillary Electrophoresis-Mass Spectrometry Open
Monoclonal antibodies (mAbs) undergo multiple post-translational modifications (PTMs), e.g., charge variants, oxidation, etc., during production and storage, necessitating evaluation of the resulting PTMs as critical quality attributes (CQ…
View article: Investigating direct current potentials that affect native protein conformation during trapped ion mobility spectrometry–mass spectrometry
Investigating direct current potentials that affect native protein conformation during trapped ion mobility spectrometry–mass spectrometry Open
Trapped ion mobility spectrometry–time‐of‐flight mass spectrometry (TIMS‐TOFMS) has emerged as a tool to study protein conformational states. In TIMS, gas‐phase ions are guided across the IM stages by applying direct current (DC) potential…
View article: A monodispersed metal-complexing peptide-based polymer for mass cytometry enabling spectral applications
A monodispersed metal-complexing peptide-based polymer for mass cytometry enabling spectral applications Open
View article: Online multimethod platform for comprehensive characterization of monoclonal antibodies in cell culture fluid from a single sample injection - Intact protein workflow
Online multimethod platform for comprehensive characterization of monoclonal antibodies in cell culture fluid from a single sample injection - Intact protein workflow Open
View article: Corrigendum to “Micro-flow size-exclusion chromatography for enhanced native mass spectrometry of proteins and protein complexes” [Anal. Chim. Acta 1266 (2023) 341324]
Corrigendum to “Micro-flow size-exclusion chromatography for enhanced native mass spectrometry of proteins and protein complexes” [Anal. Chim. Acta 1266 (2023) 341324] Open
View article: Characterizing Non-covalent Protein Complexes Using Asymmetrical Flow Field-Flow Fractionation On-Line Coupled to Native Mass Spectrometry
Characterizing Non-covalent Protein Complexes Using Asymmetrical Flow Field-Flow Fractionation On-Line Coupled to Native Mass Spectrometry Open
We report an online analytical platform based on the coupling of asymmetrical flow field-flow fractionation (AF4) and native mass spectrometry (nMS) in parallel with UV-absorbance, multi-angle light scattering (MALS), and differential-refr…
View article: Micro-flow size-exclusion chromatography for enhanced native mass spectrometry of proteins and protein complexes
Micro-flow size-exclusion chromatography for enhanced native mass spectrometry of proteins and protein complexes Open
View article: Supplementary Materials and Methods, Supplementary Schemes 1 through 3, Supplementary Figures 1 through 6 and Supplementary Table 1 from A Novel Platinum(II)–Based Bifunctional ADC Linker Benchmarked Using <sup>89</sup>Zr-Desferal and Auristatin F–Conjugated Trastuzumab
Supplementary Materials and Methods, Supplementary Schemes 1 through 3, Supplementary Figures 1 through 6 and Supplementary Table 1 from A Novel Platinum(II)–Based Bifunctional ADC Linker Benchmarked Using <sup>89</sup>Zr-Desferal and Auristatin F–Conjugated Trastuzumab Open
Description of additional methods and procedures used in the study. Description of the synthesis of the drug-linkers used in the study. Supplementary Scheme S1. Synthetic route towards Cl-Lx-DFO-Fe(III), Supplementary Scheme S2. Synthetic …
View article: Data from A Novel Platinum(II)–Based Bifunctional ADC Linker Benchmarked Using <sup>89</sup>Zr-Desferal and Auristatin F–Conjugated Trastuzumab
Data from A Novel Platinum(II)–Based Bifunctional ADC Linker Benchmarked Using <sup>89</sup>Zr-Desferal and Auristatin F–Conjugated Trastuzumab Open
Greater control is desirable in the stochastic conjugation technology used to synthesize antibody–drug conjugates (ADC). We have shown recently that a fluorescent dye can be stably conjugated to a mAb using a bifunctional platinum(II) link…
View article: Supplementary Materials and Methods, Supplementary Schemes 1 through 3, Supplementary Figures 1 through 6 and Supplementary Table 1 from A Novel Platinum(II)–Based Bifunctional ADC Linker Benchmarked Using <sup>89</sup>Zr-Desferal and Auristatin F–Conjugated Trastuzumab
Supplementary Materials and Methods, Supplementary Schemes 1 through 3, Supplementary Figures 1 through 6 and Supplementary Table 1 from A Novel Platinum(II)–Based Bifunctional ADC Linker Benchmarked Using <sup>89</sup>Zr-Desferal and Auristatin F–Conjugated Trastuzumab Open
Description of additional methods and procedures used in the study. Description of the synthesis of the drug-linkers used in the study. Supplementary Scheme S1. Synthetic route towards Cl-Lx-DFO-Fe(III), Supplementary Scheme S2. Synthetic …
View article: Data from A Novel Platinum(II)–Based Bifunctional ADC Linker Benchmarked Using <sup>89</sup>Zr-Desferal and Auristatin F–Conjugated Trastuzumab
Data from A Novel Platinum(II)–Based Bifunctional ADC Linker Benchmarked Using <sup>89</sup>Zr-Desferal and Auristatin F–Conjugated Trastuzumab Open
Greater control is desirable in the stochastic conjugation technology used to synthesize antibody–drug conjugates (ADC). We have shown recently that a fluorescent dye can be stably conjugated to a mAb using a bifunctional platinum(II) link…
View article: Micro-flow Size-Exclusion Chromatography for enhanced native Mass Spectrometry of proteins and protein complexes
Micro-flow Size-Exclusion Chromatography for enhanced native Mass Spectrometry of proteins and protein complexes Open
Native Size-exclusion chromatography (SEC) employing aqueous mobile phases with volatile salts at neutral pH combined with native mass spectrometry (nMS) is a useful tool for the characterization of proteins in their native state. However,…
View article: Studying protein structure and function by native separation–mass spectrometry
Studying protein structure and function by native separation–mass spectrometry Open
View article: Hydrophilic interaction chromatography – mass spectrometry for metabolomics and proteomics: state-of-the-art and current trends
Hydrophilic interaction chromatography – mass spectrometry for metabolomics and proteomics: state-of-the-art and current trends Open
View article: Perceptions about Research Participation among Individuals at Risk and Individuals with Premanifest Huntington’s Disease: A Survey Conducted by the European Huntington Association
Perceptions about Research Participation among Individuals at Risk and Individuals with Premanifest Huntington’s Disease: A Survey Conducted by the European Huntington Association Open
There has been great progress in Huntington’s disease (HD) research. Yet, effective treatments to halt disease before the onset of disabling symptoms are still unavailable. Scientific breakthroughs require an active and lasting commitment …
View article: Limited Lactosylation of Beta-Lactoglobulin from Cow’s Milk Exerts Strong Influence on Antigenicity and Degranulation of Mast Cells
Limited Lactosylation of Beta-Lactoglobulin from Cow’s Milk Exerts Strong Influence on Antigenicity and Degranulation of Mast Cells Open
Background: beta-lactoglobulin (BLG) is one of the major cow’s milk proteins and the most abundant allergen in whey. Heating is a common technologic treatment applied during milk transformational processes. Maillardation of BLG in the pres…
View article: Microfluidic ion stripper for removal of trifluoroacetic acid from mobile phases used in HILIC-MS of intact proteins
Microfluidic ion stripper for removal of trifluoroacetic acid from mobile phases used in HILIC-MS of intact proteins Open
View article: Probing Polyester Branching by Hybrid Trapped Ion-Mobility Spectrometry–Tandem Mass Spectrometry
Probing Polyester Branching by Hybrid Trapped Ion-Mobility Spectrometry–Tandem Mass Spectrometry Open
Trapped ion-mobility spectrometry combined with quadrupole time-of-flight mass spectrometry (TIMS-QTOFMS) was evaluated as a tool for resolving linear and branched isomeric polyester oligomers. Solutions of polyester samples were infused d…
View article: CE-MS for Proteomics and Intact Protein Analysis
CE-MS for Proteomics and Intact Protein Analysis Open
View article: Hydrophilic interaction liquid chromatography-mass spectrometry for the characterization of glycoproteins at the glycan, peptide, subunit, and intact level
Hydrophilic interaction liquid chromatography-mass spectrometry for the characterization of glycoproteins at the glycan, peptide, subunit, and intact level Open
View article: Asymmetrical flow field-flow fractionation to probe the dynamic association equilibria of β-D-galactosidase
Asymmetrical flow field-flow fractionation to probe the dynamic association equilibria of β-D-galactosidase Open
Protein dynamics play a significant role in many aspects of enzyme activity. Monitoring of structural changes and aggregation of biotechnological enzymes under native conditions is important to safeguard their properties and function. In t…
View article: Probing Protein Denaturation during Size-Exclusion Chromatography Using Native Mass Spectrometry
Probing Protein Denaturation during Size-Exclusion Chromatography Using Native Mass Spectrometry Open
Size-exclusion chromatography employing aqueous mobile phases with volatile salts at neutral pH combined with electrospray-ionization mass spectrometry (SEC-ESI-MS) is a useful tool to study proteins in their native state. However, whether…
View article: Profiling of a high mannose-type N-glycosylated lipase using hydrophilic interaction chromatography-mass spectrometry
Profiling of a high mannose-type N-glycosylated lipase using hydrophilic interaction chromatography-mass spectrometry Open
View article: Rapid forensic chemical classification of confiscated flash banger fireworks using capillary electrophoresis
Rapid forensic chemical classification of confiscated flash banger fireworks using capillary electrophoresis Open
View article: Clinical Metabolomics: Expanding the Metabolome Coverage Using Advanced Analytical Techniques
Clinical Metabolomics: Expanding the Metabolome Coverage Using Advanced Analytical Techniques Open
This review article discusses the novel separation and detection strategies that are considered promising in clinical metabolomics to enhance the metabolome coverage. It includes hydrophilic interaction chromatography (HILIC), supercritica…
View article: Computer-aided gradient optimization of hydrophilic interaction liquid chromatographic separations of intact proteins and protein glycoforms
Computer-aided gradient optimization of hydrophilic interaction liquid chromatographic separations of intact proteins and protein glycoforms Open
View article: Affinity profiling of monoclonal antibody and antibody-drug-conjugate preparations by coupled liquid chromatography-surface plasmon resonance biosensing
Affinity profiling of monoclonal antibody and antibody-drug-conjugate preparations by coupled liquid chromatography-surface plasmon resonance biosensing Open