Robbie Barbero
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View article: A 1-year study on SARS-CoV-2 variant shifts in wastewater using dPCR: comparison with clinical and GISAID data
A 1-year study on SARS-CoV-2 variant shifts in wastewater using dPCR: comparison with clinical and GISAID data Open
Wastewater testing can be used to monitor SARS-CoV-2 infections in communities. Data from PCR-based wastewater testing are usually available to public health authorities within 5–7 days after excreta and other body fluids enter the sewer. …
View article: Novel dPCR-Based Approach for SARS-CoV-2 Variant Detection and Monitoring in Wastewater: A Multi-State Comparison with Clinical Genotyping and GISAID Sequencing Data
Novel dPCR-Based Approach for SARS-CoV-2 Variant Detection and Monitoring in Wastewater: A Multi-State Comparison with Clinical Genotyping and GISAID Sequencing Data Open
Wastewater testing has emerged as an effective tool for monitoring levels of SARS-CoV-2 infection in sewered communities. As of July 2024, PCR-based methods continue to be the most widely used methods in wastewater surveillance (1–3). Data…
View article: Comparison of Nanotrap® Microbiome A Particles, membrane filtration, and skim milk workflows for SARS-CoV-2 concentration in wastewater
Comparison of Nanotrap® Microbiome A Particles, membrane filtration, and skim milk workflows for SARS-CoV-2 concentration in wastewater Open
Introduction Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) RNA monitoring in wastewater has become an important tool for Coronavirus Disease 2019 (COVID-19) surveillance. Grab (quantitative) and passive samples (qualitative)…
View article: Magnetic hydrogel particles improve nanopore sequencing of SARS-CoV-2 and other respiratory viruses
Magnetic hydrogel particles improve nanopore sequencing of SARS-CoV-2 and other respiratory viruses Open
View article: Improved Detection of Herpesviruses from Diluted Vitreous Specimens Using Hydrogel Particles
Improved Detection of Herpesviruses from Diluted Vitreous Specimens Using Hydrogel Particles Open
Infectious uveitis is a sight-threatening infection commonly caused by herpesviruses. Vitreous humor is often collected for molecular confirmation of the causative agent during vitrectomy and mixed in large volumes of buffered saline, dilu…
View article: Characterization and Optimization of Nanotrap® Microbiome Particlemethods for Concentrating SARS-CoV-2 from Small Volume Wastewater Samples
Characterization and Optimization of Nanotrap® Microbiome Particlemethods for Concentrating SARS-CoV-2 from Small Volume Wastewater Samples Open
View article: Nanotrap Particles Improve Nanopore Sequencing of SARS-CoV-2 and Other Respiratory Viruses
Nanotrap Particles Improve Nanopore Sequencing of SARS-CoV-2 and Other Respiratory Viruses Open
Presented here is a magnetic hydrogel particle enabled workflow for capturing and concentrating SARS-CoV-2 from diagnostic remnant swab samples that significantly improves sequencing results using the Oxford Nanopore Technologies MinION se…
View article: Hydrogel particles improve detection of SARS-CoV-2 RNA from multiple sample types
Hydrogel particles improve detection of SARS-CoV-2 RNA from multiple sample types Open
View article: Nanotrap<sup>®</sup>particles improve detection of SARS-CoV-2 for pooled sample methods, extraction-free saliva methods, and extraction-free transport medium methods
Nanotrap<sup>®</sup>particles improve detection of SARS-CoV-2 for pooled sample methods, extraction-free saliva methods, and extraction-free transport medium methods Open
Here we present a rapid and versatile method for capturing and concentrating SARS-CoV-2 from transport medium and saliva using affinity-capture magnetic hydrogel particles. We demonstrate that the method concentrates virus prior to RNA ext…