Robert A. Briggaman
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View article: Neonatal Foreskin Substrate Has Limitations for the Immunofluorescent Screening of Monoclonal Antibodies
Neonatal Foreskin Substrate Has Limitations for the Immunofluorescent Screening of Monoclonal Antibodies Open
Two monoclonal antibodies to type IV collagen showed a marked decrease in the labeling of the dermal-epidermal junction of neonatal foreskin while the basement membrane around dermal blood vessels was brightly stained. In contrast, these a…
View article: A Mouse Monoclonal Antibody Against a Newly Discovered Basement Membrane Component, the Epidermolysis Bullosa Acquisita Antigen
A Mouse Monoclonal Antibody Against a Newly Discovered Basement Membrane Component, the Epidermolysis Bullosa Acquisita Antigen Open
A mouse monoclonal antibody, H3a, directed against the newly described epidermolysis bullosa acquisita (EBA) antigen was obtained using hybridoma techniques. The distribution of the monoclonal antibody is identical to that of the polyclona…
View article: Monoclonal Antibodies to Anchoring Fibrils for the Diagnosis of Epidermolysis Bullosa
Monoclonal Antibodies to Anchoring Fibrils for the Diagnosis of Epidermolysis Bullosa Open
Murine monoclonal antibodies to human anchoring fibrils reacted with human and monkey cervix, tongue, esophagus, and vagina. Rat, mouse, and guinea pig tissues were negative. In 11 patients with dystrophic recessive epidermolysis bullosa t…
View article: Biochemical Composition of the Epidermal-dermal Junction and Other Basement Membrane
Biochemical Composition of the Epidermal-dermal Junction and Other Basement Membrane Open
The epidermo-dermal junction ultrastructurally appears to be high organized, presenting 4 layers: (1) the basal cell plasma membrane, a trilaminar layer with special attachment structures (hemidesmosomes); (2) lamina lucida (rara) an elect…
View article: Absence of Specific Histologic Changes in Guinea Pig Skin Treated With Bullous Pemphigoid Antibodies
Absence of Specific Histologic Changes in Guinea Pig Skin Treated With Bullous Pemphigoid Antibodies Open
Previous studies have reported that intradermal injections of bullous pemphigoid antibodies into guinea pigs can reproduce the histologic and immunohistologic features of bullous pemphigoid lesions. In this study we examined this model to …
View article: Adherens Junctions: Demonstration in Human Epidermis
Adherens Junctions: Demonstration in Human Epidermis Open
Adherens junctions are intercellular and cell-matrix junctions that, like desmosomes and hemidesmosomes, mediate adhesion of cells to each other or to matrix structures. These junctions have been detected recently in cultured human keratin…
View article: Intracytoplasmic Retention of Type VII Collagen and Dominant Dystrophic Epidermolysis Bullosa: Reversal of Defect Following Cessation of or Marked Improvement in Disease Activity
Intracytoplasmic Retention of Type VII Collagen and Dominant Dystrophic Epidermolysis Bullosa: Reversal of Defect Following Cessation of or Marked Improvement in Disease Activity Open
It has been recently shown that the presence of perinuclear "stellate bodies" within the epidermis in patients with a form of dominant dystrophic epidermolysis bullosa named "transient bullous dermolysis of the newborn" corresponds to coll…
View article: Autoantibodies to Type VII Collagen Recognize Epitopes in a Fibronectin-Like Region of the Noncollagenous (NC1) Domain
Autoantibodies to Type VII Collagen Recognize Epitopes in a Fibronectin-Like Region of the Noncollagenous (NC1) Domain Open
Autoantibodies to type VII collagen are characteristic of the blistering diseases epidermolysis bullosa acquisita and bullous systemic lupus erythematosus (SLE). Blisters in those diseases are due to defective adhesion of the lamina densa …
View article: Functional Heterogeneity of Immune Complexes in Epidermolysis Bullosa Acquisita
Functional Heterogeneity of Immune Complexes in Epidermolysis Bullosa Acquisita Open
Epidermolysis bullosa acquisita is an inflammatory subepidermal bullous disease characterized by circulating and tissue-bound complement-binding anti-basement membrane zone autoantibodies to type VII procollagen. Lesions are characterized …
View article: Collagen Telopeptides (Cross-Linking Sites) Play a Role in Collagen Gel Lattice Contraction
Collagen Telopeptides (Cross-Linking Sites) Play a Role in Collagen Gel Lattice Contraction Open
Solubilized interstitial collagens will form a fibrillar, gel-like lattice when brought to physiologic conditions. In the presence of human dermal fibroblasts the collagen lattice will contract. The rate of contraction can be determined by…
View article: Characterization of “Neo-Dermis” Formation Beneath Cultured Human Epidermal Autografts Transplanted on Muscle Fascia
Characterization of “Neo-Dermis” Formation Beneath Cultured Human Epidermal Autografts Transplanted on Muscle Fascia Open
Cultured human keratinocyte autografts were transplanted to burn wounds that had been completely excised down to muscle fascia such that all cutaneous elements were removed from the wounds. Healing autografts were biopsied from days 6-153 …
View article: Epidermolysis Bullosa Acquisita Antigen, a Major Cutaneous Basement Membrane Component, Is Synthesized by Human Dermal Fibroblasts and Other Cutaneous Tissues
Epidermolysis Bullosa Acquisita Antigen, a Major Cutaneous Basement Membrane Component, Is Synthesized by Human Dermal Fibroblasts and Other Cutaneous Tissues Open
The epidermolysis bullosa acquisita (EBA) antigen is identified as 2 chains: a 290,000-dalton protein and a less prominent 145,000-dalton protein. The 290,000-dalton chain is synthesized by human keratinocytes in culture. In this study, we…
View article: Epidermolysis Bullosa Acquisita Antigen: Relationship Between the Collagenase-Sensitive and -Insensitive Domains
Epidermolysis Bullosa Acquisita Antigen: Relationship Between the Collagenase-Sensitive and -Insensitive Domains Open
To clarify the relationship of the 290 and 145 kDa chains of the epidermolysis bullosa acquisita (EBA) antigen, we subjected urea extracts of skin basement membrane zone (BMZ) proteins and isolated 290 and 145 kDa chains of the EBA antigen…
View article: Noncollagenous (NC1) Domain of Collagen VII Resembles Multidomain Adhesion Proteins Involved in Tissue-Specific Organization of Extracellular Matrix
Noncollagenous (NC1) Domain of Collagen VII Resembles Multidomain Adhesion Proteins Involved in Tissue-Specific Organization of Extracellular Matrix Open
Type VII collagen (C7) is a stratified squamous epithelial basement membrane protein composed of three identical alpha chains, each consisting of a 145-kDa amino-terminal noncollagenous (NC1) domain and a 145-kDa carboxyl-terminal collagen…
View article: Epidermolysis Bullosa Acquisita Antigen, a New Major Component of Cutaneous Basement Membrane, Is a Glycoprotein with Collagenous Domains
Epidermolysis Bullosa Acquisita Antigen, a New Major Component of Cutaneous Basement Membrane, Is a Glycoprotein with Collagenous Domains Open
The epidermolysis bullosa acquisita antigen is a major constituent of the basement membrane zone beneath stratified squamous epithelium. The antigen which is recognized in extracts of skin basement membrane by Western blot analysis with po…
View article: Epidermolysis Bullosa Acquisita: Ultrastructural and Immunological Studies
Epidermolysis Bullosa Acquisita: Ultrastructural and Immunological Studies Open
Four patients with epidermolysis bullosa acquisita were investigated using immunofluorescence, routine electron microscopic and immunoelectron microscopic techniques. Immunofluorescence studies demonstrated linear immunoglobulin and comple…
View article: Production of Fibronectin by Epithelium in a Skin Equivalent
Production of Fibronectin by Epithelium in a Skin Equivalent Open
Although human keratinocytes in vitro have been shown to produce fibronectin, whether keratinocytes can contribute fibronectin to the dermal-epidermal junction or wound matrix is unknown. In order to approach this problem experimentally, w…
View article: Double Immunofluorescence Microscopy: A Method for Localizing Immune Deposits in Skin Diseases Associated with Linear Basement Membrane Zone Immunofluorescence
Double Immunofluorescence Microscopy: A Method for Localizing Immune Deposits in Skin Diseases Associated with Linear Basement Membrane Zone Immunofluorescence Open
Direct immunofluorescence microscopy has shown that a linear pattern of immunoglobulin and/or complement deposition at the cutaneous basement membrane zone is a characteristic feature in a number of acquired bullous diseases and is occasio…
View article: Calcium-induced assembly of adherens junctions in keratinocytes
Calcium-induced assembly of adherens junctions in keratinocytes Open
Extracellular calcium concentration has been shown to control the stratification of cultured keratinocytes, presumably by regulation of formation of desmosomes. Previous studies have shown that keratinocytes cultured in medium containing 0…
View article: Epidermal-dermal Interactions in Adult Skin
Epidermal-dermal Interactions in Adult Skin Open
Epidermal-dermal interactions are important determinants of embryonic development in skin. This review examines the role of such epidermal-dermal interactions in the conservation of epithelial specificity in adult skin. The basic epidermal…
View article: Hailey-Hailey Disease Keratinocytes: Normal Assembly of Cell-Cell Junctions In Vitro
Hailey-Hailey Disease Keratinocytes: Normal Assembly of Cell-Cell Junctions In Vitro Open
The blisters in the inherited disorder, Hailey-Hailey disease, may be caused by defective epidermal junctional complexes. We evaluated these structural complexes in vivo and in vitro. We induced a vesicular lesion in the apparently normal …