Robert Bücker
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View article: The Transformative Effect of Electron Diffraction
The Transformative Effect of Electron Diffraction Open
Crystal structures are critical to unambiguously determine a compound’s identity, absolute configuration, molecular connectivity and crystal form, as well as whether polymorphs coexist or how the solid-state structure may explain a compoun…
View article: Electron diffraction captures high-resolution structures from <i>in vivo</i> protein nanocrystals of <i>Bacillus thuringiensis</i>
Electron diffraction captures high-resolution structures from <i>in vivo</i> protein nanocrystals of <i>Bacillus thuringiensis</i> Open
Bacillus thuringiensis is one of the most widely used biopesticides worldwide owing to the highly specific pesticidal-proteins various strains produce in the form of nanocrystals. Structure determination of such crystals remains difficult …
View article: Post-COVID Extension of an Online Crystallography School
Post-COVID Extension of an Online Crystallography School Open
Rigaku ran a series of practical schools to address the loss of crystallographic training opportunities resulting from the cancellation of conventional schools around the world due to the COVID-19 pandemic. With the return to normalcy, we …
View article: Challenges and Victories: Variable Temperature MicroED on Small Molecules
Challenges and Victories: Variable Temperature MicroED on Small Molecules Open
Since our launch of the XtaLAB Synergy-ED in 2021, users have produced hundreds of new and re-visited structures via MicroED, including over 500 unique structures from Rigaku application labs alone. Most of the early studies were conducted…
View article: Unit-cell parameters determination from a set of independent electron diffraction zonal patterns
Unit-cell parameters determination from a set of independent electron diffraction zonal patterns Open
Due to the short de Broglie wavelength of electrons compared with X-rays, the curvature of their Ewald sphere is low, and individual electron diffraction patterns are nearly flat in reciprocal space. As a result, a reliable unit-cell deter…
View article: STEM SerialED: achieving high-resolution data for <i>ab initio</i> structure determination of beam-sensitive nanocrystalline materials
STEM SerialED: achieving high-resolution data for <i>ab initio</i> structure determination of beam-sensitive nanocrystalline materials Open
Serial electron diffraction (SerialED), which applies a snapshot data acquisition strategy for each crystal, was introduced to tackle the problem of radiation damage in the structure determination of beam-sensitive materials by three-dimen…
View article: STEM SerialED: achieving high-resolution data for ab initio structure determination of beam-sensitive nanocrystalline materials
STEM SerialED: achieving high-resolution data for ab initio structure determination of beam-sensitive nanocrystalline materials Open
Serial electron diffraction (SerialED), which applies a snapshot data acquisition strategy on each crystal, was introduced to tackle the problem of radiation damage in the structure determination of beam-sensitive materials by three-dimens…
View article: Making the Most of 3D Electron Diffraction: Best Practices to Handle a New Tool
Making the Most of 3D Electron Diffraction: Best Practices to Handle a New Tool Open
Along with the adoption of three-dimensional electron diffraction (3D ED/MicroED) as a mainstream tool for structure determination from sub-micron single crystals, questions about best practices regarding each step along the workflow, from…
View article: CCDC 2243533: Experimental Crystal Structure Determination
CCDC 2243533: Experimental Crystal Structure Determination Open
An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available …
View article: Structural Analysis and Intrinsic Enzyme Mimicking Activities of Ligand‐Free PtAg Nanoalloys (Small 19/2023)
Structural Analysis and Intrinsic Enzyme Mimicking Activities of Ligand‐Free PtAg Nanoalloys (Small 19/2023) Open
Ligand-Free PtAg Nanoalloys In article number 2206772, Indranath Chakraborty and co-workers use the atomic pair-distribution function combined with powder X-ray diffraction and transmission electron microscopy for structural analysis of li…
View article: Structural Analysis and Intrinsic Enzyme Mimicking Activities of Ligand‐Free PtAg Nanoalloys
Structural Analysis and Intrinsic Enzyme Mimicking Activities of Ligand‐Free PtAg Nanoalloys Open
Nanozymes are nanomaterials with biocatalytic properties under physiological conditions and are one class of artificial enzymes to overcome the high cost and low stability of natural enzymes. However, surface ligands on nanomaterials will …
View article: Amyloid fibril from the antimicrobial peptide uperin 3.5
Amyloid fibril from the antimicrobial peptide uperin 3.5 Open
EMPIAR, the Electron Microscopy Public Image Archive centered at EMBL-EBI, is a public resource for raw electron microscopy images related to EMDB, contains micrographs, particle sets and tilt-series.
View article: Amyloid fibril from the antimicrobial peptide aurein 3.3
Amyloid fibril from the antimicrobial peptide aurein 3.3 Open
EMPIAR, the Electron Microscopy Public Image Archive centered at EMBL-EBI, is a public resource for raw electron microscopy images related to EMDB, contains micrographs, particle sets and tilt-series.
View article: The effect of secondary electrons on radiolysis as observed by in liquid TEM: The role of window material and electrical bias
The effect of secondary electrons on radiolysis as observed by in liquid TEM: The role of window material and electrical bias Open
The effect of window material on electron beam induced phenomena in liquid phase electron microscopy (LPEM) is an interesting yet under-explored subject. We have studied the differences of electron beam induced gold nanoparticle (AuNP) gro…
View article: The Cryo-EM Structures of two Amphibian Antimicrobial Cross-β Amyloid Fibrils
The Cryo-EM Structures of two Amphibian Antimicrobial Cross-β Amyloid Fibrils Open
The amyloid-antimicrobial link hypothesis is based on antimicrobial properties found in human amyloids involved in neurodegenerative and systemic diseases, along with amyloidal structural properties found in antimicrobial peptides (AMPs) a…
View article: Serial Electron Crystallography: New Developments for Data Collection and Analysis
Serial Electron Crystallography: New Developments for Data Collection and Analysis Open
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View article: Serial Electron Diffraction Data Processing With diffractem and CrystFEL
Serial Electron Diffraction Data Processing With diffractem and CrystFEL Open
Serial electron diffraction (SerialED) is an emerging technique, which applies the snapshot data-collection mode of serial X-ray crystallography to three-dimensional electron diffraction (3D Electron Diffraction), forgoing the conventional…
View article: Serial femtosecond and serial synchrotron crystallography can yield data of equivalent quality: A systematic comparison
Serial femtosecond and serial synchrotron crystallography can yield data of equivalent quality: A systematic comparison Open
Systematic comparison between two serial crystallography methods shows that both enable time-resolved measurements.
View article: Serial Electron Diffraction Data Processing with diffractem and CrystFEL
Serial Electron Diffraction Data Processing with diffractem and CrystFEL Open
Serial electron diffraction (SerialED) is an emerging technique, which applies the snapshot data-collection mode of serial X-ray crystallography to three-dimensional electron diffraction (3D ED), forgoing the conventional rotation method. …
View article: Serial femtosecond and serial synchrotron crystallography yield data of equivalent quality: a systematic comparison
Serial femtosecond and serial synchrotron crystallography yield data of equivalent quality: a systematic comparison Open
For the two proteins myoglobin (MB) and fluoroacetate dehalogenase (FAcD), we present a systematic comparison of crystallographic diffraction data collected by serial femtosecond (SFX) and serial synchrotron crystallography (SSX). To maxim…
View article: Serial protein crystallography in an electron microscope
Serial protein crystallography in an electron microscope Open
Serial X-ray crystallography at free-electron lasers allows to solve biomolecular structures from sub-micron-sized crystals. However, beam time at these facilities is scarce, and involved sample delivery techniques are required. On the oth…
View article: Dedicated electron source for serial electron crystallography
Dedicated electron source for serial electron crystallography Open
Work in electron-based macromolecular structure determination has grown in importance with the recent developments in rotation electron crystallography [1].It was recently shown that serial electron crystallography (serialED) can be implem…
View article: Serial protein crystallography in an S/TEM
Serial protein crystallography in an S/TEM Open
Serial crystallography, where diffraction snapshots of a large ensemble of randomly oriented crystals are taken, evades the cumulative damage inherent to rotation diffraction techniques.This approach has facilitated the use of sub-micron c…
View article: Electron Ptychography of Single Biological Macromolecules
Electron Ptychography of Single Biological Macromolecules Open
Journal Article Electron Ptychography of Single Biological Macromolecules Get access Philipp M Pelz, Philipp M Pelz Max Planck Institute for the Structure and Dynamics of Matter, Hamburg, GermanyMaterials Science and Engineering, UC Berkel…
View article: Serial protein crystallography in an electron microscope
Serial protein crystallography in an electron microscope Open
Serial X-ray crystallography at free-electron lasers allows to solve biomolecular structures from sub-micron-sized crystals. However, beam time at these facilities is scarce, and involved sample delivery techniques are required. On the oth…