Robert C. Murphy
YOU?
Author Swipe
View article: Technical recommendations for analyzing oxylipins by liquid chromatography–mass spectrometry
Technical recommendations for analyzing oxylipins by liquid chromatography–mass spectrometry Open
Several oxylipins are potent lipid mediators that regulate diverse aspects of health and disease and whose quantitative analysis by liquid chromatography–mass spectrometry (LC-MS) presents substantial technical challenges. As members of th…
View article: Diet‐Induced Obesity Blunts Sensitivity of Intestinal Enteric Neurons: FIRST Evidence of Modulation of Activity of Enteric Neurons by Luminal Nutrients
Diet‐Induced Obesity Blunts Sensitivity of Intestinal Enteric Neurons: FIRST Evidence of Modulation of Activity of Enteric Neurons by Luminal Nutrients Open
The enteric nervous system (ENS) has well‐established roles in gut motility, epithelial secretion, and blood flow. However, its role in luminal nutrient sensing remains elusive. Given that the nerve endings of enteric neurons terminate at …
View article: Diet-Induced Obesity Differentially Regulates Intestinal Nutrient Sensing by Enteric Neurons: Evidence of Direct Neuronal Activation by Luminal Nutrients
Diet-Induced Obesity Differentially Regulates Intestinal Nutrient Sensing by Enteric Neurons: Evidence of Direct Neuronal Activation by Luminal Nutrients Open
Objectives: Background: Our published data shows that blocking intestinal cholinergic neurotransmission in diet-induced obese (DIO) mice resulted in significant weight loss and robust improvements in hyperglycemia, hyperlipidemia, and gluc…
View article: Data from Biomarkers for Personalizing Omega-3 Fatty Acid Dosing
Data from Biomarkers for Personalizing Omega-3 Fatty Acid Dosing Open
Prostaglandin E2 (PGE2) has been linked to a higher risk of colorectal cancer. PGE2 in colon tissue can be reduced by increasing dietary eicosapentaenoic acid (EPA). The dose-dependent relationships between dietary EPA, serum EPA:arachidon…
View article: Supplementary Table S2 from Biomarkers for Personalizing Omega-3 Fatty Acid Dosing
Supplementary Table S2 from Biomarkers for Personalizing Omega-3 Fatty Acid Dosing Open
Table S2 shows the molar % of fatty acids in the colon at the fed EPA:AA ratios.
View article: Data from Chemotherapeutic Agents Subvert Tumor Immunity by Generating Agonists of Platelet-Activating Factor
Data from Chemotherapeutic Agents Subvert Tumor Immunity by Generating Agonists of Platelet-Activating Factor Open
Oxidative stress suppresses host immunity by generating oxidized lipid agonists of the platelet-activating factor receptor (PAF-R). Because many classical chemotherapeutic drugs induce reactive oxygen species (ROS), we investigated whether…
View article: Supplementary Methods from Chemotherapeutic Agents Subvert Tumor Immunity by Generating Agonists of Platelet-Activating Factor
Supplementary Methods from Chemotherapeutic Agents Subvert Tumor Immunity by Generating Agonists of Platelet-Activating Factor Open
Supplementary Methods. Supplementary Mass spectrometric methodology
View article: Supplemental Figures S1-S6 from Chemotherapeutic Agents Subvert Tumor Immunity by Generating Agonists of Platelet-Activating Factor
Supplemental Figures S1-S6 from Chemotherapeutic Agents Subvert Tumor Immunity by Generating Agonists of Platelet-Activating Factor Open
Supplemental Figures S1-S6
View article: System for characterizing semiconductor materials and photovoltaic devices through calibration
System for characterizing semiconductor materials and photovoltaic devices through calibration Open
A method and apparatus for measuring characteristics of a piece of material, typically semiconductor materials including photovoltaic devices. The characteristics may include dislocation defect density, grain boundaries, reflectance, exter…
View article: AB085. SOH22ABS232. Does previous fragility fracture impact upon mortality in a hip fracture cohort?—A retrospective study
AB085. SOH22ABS232. Does previous fragility fracture impact upon mortality in a hip fracture cohort?—A retrospective study Open
Background: Hip fractures remain a common but burdensome injury among older patients. Many factors influence a patient’s risk of hip fracture and their subsequent risk of death. Fragility fractures are described as fractures resulting from…
View article: The SARS-CoV2 envelope differs from host cells, exposes procoagulant lipids, and is disrupted in vivo by oral rinses
The SARS-CoV2 envelope differs from host cells, exposes procoagulant lipids, and is disrupted in vivo by oral rinses Open
The lipid envelope of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an essential component of the virus; however, its molecular composition is undetermined. Addressing this knowledge gap could support the design of antivi…
View article: Formation, Signaling and Occurrence of Specialized Pro-Resolving Lipid Mediators—What is the Evidence so far?
Formation, Signaling and Occurrence of Specialized Pro-Resolving Lipid Mediators—What is the Evidence so far? Open
Formation of specialized pro-resolving lipid mediators (SPMs) such as lipoxins or resolvins usually involves arachidonic acid 5-lipoxygenase (5-LO, ALOX5) and different types of arachidonic acid 12- and 15-lipoxygenating paralogues (15-LO1…
View article: The SARS-CoV2 envelope is distinct from host membranes, exposes pro-coagulant lipids, and can be inactivated <i>in vivo</i> by surfactant-containing oral rinses
The SARS-CoV2 envelope is distinct from host membranes, exposes pro-coagulant lipids, and can be inactivated <i>in vivo</i> by surfactant-containing oral rinses Open
The lipid envelope of SARS-CoV2 is an essential component of the virus, however its molecular composition is unknown. Addressing this knowledge gap could support the design of anti-viral agents, and further understanding of viral interacti…
View article: Book Review: The Gold Standard: Retrospect and Prospect
Book Review: The Gold Standard: Retrospect and Prospect Open
_The Gold Standard_ is a useful collection for those readers who want an evenhanded analysis of the monetary mechanism, in contrast to the casual dismissals that the gold standard currently receives even from professional economists. Put o…
View article: Relationships between gut microbiota, red meat consumption and colorectal cancer
Relationships between gut microbiota, red meat consumption and colorectal cancer Open
The temperature cooking of meat such as grilling and smoking contribute to the formation of mutagenic compounds including heterocyclic amines and polycyclic aromatic hydrocarbons. Heme iron in red meat is involved in the formation of N-nit…
View article: Failure to apply standard limit-of-detection or limit-of-quantitation criteria to specialized pro-resolving mediator analysis incorrectly characterizes their presence in biological samples.
Failure to apply standard limit-of-detection or limit-of-quantitation criteria to specialized pro-resolving mediator analysis incorrectly characterizes their presence in biological samples. Open
Specialized pro-resolving mediators (SPM) derived from oxygenation of long chain polyunsaturated fatty acids (PUFA) were originally described by Serhan and colleagues and have been proposed as mediators of inflammation resolution. Families…
View article: Lipoprotein Lipase Regulates Microglial Lipid Droplet Accumulation
Lipoprotein Lipase Regulates Microglial Lipid Droplet Accumulation Open
Microglia become increasingly dysfunctional with aging and contribute to the onset of neurodegenerative disease (NDs) through defective phagocytosis, attenuated cholesterol efflux, and excessive secretion of pro-inflammatory cytokines. Dys…
View article: Lipidomic and transcriptional analysis of the linoleoyl-omega-hydroxyceramide biosynthetic pathway in human psoriatic lesions
Lipidomic and transcriptional analysis of the linoleoyl-omega-hydroxyceramide biosynthetic pathway in human psoriatic lesions Open
A complex assembly of lipids including fatty acids, cholesterol, and ceramides is vital to the integrity of the mammalian epidermal barrier. The formation of this barrier requires oxidation of the substrate fatty acid, linoleic acid (LA), …
View article: Steps Toward Minimal Reporting Standards for Lipidomics Mass Spectrometry in Biomedical Research Publications
Steps Toward Minimal Reporting Standards for Lipidomics Mass Spectrometry in Biomedical Research Publications Open
Lipids in blood and tissues can serve as markers of normal and pathophysiological function in humans and can even reflect functions in specific tissues and organs. Lipidomics describes the analysis of large numbers of lipids using mass spe…
View article: Effectiveness of a Statewide Abusive Head Trauma Prevention Program in North Carolina
Effectiveness of a Statewide Abusive Head Trauma Prevention Program in North Carolina Open
Abusive head trauma (AHT) is a serious condition, with an incidence of approximately 30 cases per 100 000 person-years in the first year of life.
View article: Update on LIPID MAPS classification, nomenclature, and shorthand notation for MS-derived lipid structures
Update on LIPID MAPS classification, nomenclature, and shorthand notation for MS-derived lipid structures Open
A comprehensive and standardized system to report lipid structures analyzed by MS is essential for the communication and storage of lipidomics data. Herein, an update on both the LIPID MAPS classification system and shorthand notation of l…
View article: Oxylipin metabolism is controlled by mitochondrial β-oxidation during bacterial inflammation
Oxylipin metabolism is controlled by mitochondrial β-oxidation during bacterial inflammation Open
Oxylipins are potent biological mediators requiring strict control, but how they are removed en masse during infection and inflammation is unknown. Here we show that lipopolysaccharide (LPS) dynamically enhances oxylipin removal via mitoch…
View article: A Reliable and Accurate Solution to the Induced Fit Docking Problem for Protein-Ligand Binding
A Reliable and Accurate Solution to the Induced Fit Docking Problem for Protein-Ligand Binding Open
We present a reliable and accurate solution to the induced fit docking problem for protein-ligand binding by combining ligand-based pharmacophore docking (Phase), rigid receptor docking (Glide), and protein structure prediction (Prime) wit…
View article: A Reliable and Accurate Solution to the Induced Fit Docking Problem for Protein-Ligand Binding
A Reliable and Accurate Solution to the Induced Fit Docking Problem for Protein-Ligand Binding Open
We present a reliable and accurate solution to the induced fit docking problem for protein-ligand binding by combining ligand-based pharmacophore docking (Phase), rigid receptor docking (Glide), and protein structure prediction (Prime) wit…
View article: Lipid mass spectrometry: A path traveled for 50 years
Lipid mass spectrometry: A path traveled for 50 years Open
In the middle of the 1960s, I began graduate school and at the same time started on the path of using mass spectrometry to gain insight into various aspects of lipid biochemistry. This was not a straight path but one that went from organic…
View article: Potential Role of Oral Rinses Targeting the Viral Lipid Envelope in SARS-CoV-2 Infection
Potential Role of Oral Rinses Targeting the Viral Lipid Envelope in SARS-CoV-2 Infection Open
Emerging studies increasingly demonstrate the importance of the throat and salivary glands as sites of virus replication and transmission in early COVID-19 disease. SARS-CoV-2 is an enveloped virus, characterized by an outer lipid membrane…