Robert C. Piper
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View article: Insulated <i>piggyBac</i> and FRT vectors for engineering transgenic homozygous and heterozygous eHAP cells
Insulated <i>piggyBac</i> and FRT vectors for engineering transgenic homozygous and heterozygous eHAP cells Open
Transgene expression in eHAP cells, a haploid cell line commonly used to generate gene knockouts, is difficult due to its low transfection efficiency and poor expression of integrated transgenes. To enable simple and reliable transgene exp…
View article: Charcot-Marie-Tooth disease type 1E: Clinical Natural History and Molecular Impact of<i>PMP22</i>Variants
Charcot-Marie-Tooth disease type 1E: Clinical Natural History and Molecular Impact of<i>PMP22</i>Variants Open
Charcot-Marie-Tooth disease type 1E (CMT1E) is a rare, autosomal dominant peripheral neuropathy caused by missense variants, deletions, and truncations within the peripheral myelin protein-22 ( PMP22 ) gene. CMT1E phenotypes vary depending…
View article: K48-ubiquitin-activated proteases cut-up post-ER proteins
K48-ubiquitin-activated proteases cut-up post-ER proteins Open
Polyubiquitin chains, linked via K48 or K63 of ubiquitin, target membrane proteins in the secretory system to degradative pathways. However it’s unclear whether these linkage isomers are functionally interchangeable. Here we show that for …
View article: Disrupting the transmembrane domain interface between PMP22 and MPZ causes peripheral neuropathy
Disrupting the transmembrane domain interface between PMP22 and MPZ causes peripheral neuropathy Open
Peripheral Myelin Protein 22 (PMP22) and MPZ are abundant myelin membrane proteins in Schwann cells. The MPZ adhesion protein holds myelin wraps together across the intraperiod line. PMP22 is a tetraspan protein belonging to the Claudin su…
View article: Loss of HD-PTP function results in lipodystrophy, defective cellular signaling and altered lipid homeostasis
Loss of HD-PTP function results in lipodystrophy, defective cellular signaling and altered lipid homeostasis Open
His domain protein tyrosine phosphatase (HD-PTP; also known as PTPN23) facilitates function of the endosomal sorting complexes required for transport (ESCRTs) during multivesicular body (MVB) formation. To uncover its role in physiological…
View article: Insulated<i>piggyBac</i>and FRT vectors for engineering transgenic homozygous and heterozygous eHAP cells
Insulated<i>piggyBac</i>and FRT vectors for engineering transgenic homozygous and heterozygous eHAP cells Open
Transgene expression in eHAP cells, a haploid cell line popularly used to generate gene knockouts, is difficult owing to its low transfection efficiency and propensity for silencing integrated transgenes. To simplify transgene expression, …
View article: Editorial: Regulation of hormone and growth factor signalling by ubiquitin and ubiquitin-like protein modifications
Editorial: Regulation of hormone and growth factor signalling by ubiquitin and ubiquitin-like protein modifications Open
Editorial: Regulation of hormone and growth factor signalling by ubiquitin and ubiquitin-like protein modifications
View article: Disrupting the transmembrane domain interface between PMP22 and MPZ causes peripheral neuropathy
Disrupting the transmembrane domain interface between PMP22 and MPZ causes peripheral neuropathy Open
PMP22 and MPZ are abundant myelin membrane proteins in Schwann cells. The MPZ adhesion protein holds myelin wraps together across the intraperiod line. PMP22 is a tetraspan protein belonging to the Claudin superfamily. Loss of either MPZ o…
View article: Beta-subunit-eliminated eHAP expression (BeHAPe) cells reveal subunit regulation of the cardiac voltage-gated sodium channel
Beta-subunit-eliminated eHAP expression (BeHAPe) cells reveal subunit regulation of the cardiac voltage-gated sodium channel Open
Voltage-gated sodium (NaV) channels drive the upstroke of the action potential and are comprised of a pore-forming α-subunit and regulatory β-subunits. The β-subunits modulate the gating, trafficking, and pharmacology of the α-subunit. The…
View article: Homomeric interactions of the MPZ Ig domain and their relation to Charcot-Marie-Tooth disease
Homomeric interactions of the MPZ Ig domain and their relation to Charcot-Marie-Tooth disease Open
Mutations in MPZ (myelin protein zero) can cause demyelinating early-onset Charcot-Marie-Tooth type 1B disease or later onset type 2I/J disease characterized by axonal degeneration, reflecting the diverse roles of MPZ in Schwann cells. MPZ…
View article: Beta-subunit-eliminated eHAP expression (BeHAPe) cells reveal new properties of the cardiac voltage-gated sodium channel
Beta-subunit-eliminated eHAP expression (BeHAPe) cells reveal new properties of the cardiac voltage-gated sodium channel Open
Voltage-gated sodium (Na V ) channels drive the upstroke of the action potential and are comprised of a pore-forming α-subunit and regulatory β-subunits. The β-subunits modulate the gating, trafficking, and pharmacology of the α-subunit. T…
View article: The Institutional Drivers Contributing to Billionaire Wealth at the Sector Level
The Institutional Drivers Contributing to Billionaire Wealth at the Sector Level Open
Over the last 50 years (the period of neoliberalism) the national wealth of the United States reached unprecedented levels. Despite this dramatic increase in national wealth, an increasing amount of American wealth has found its way into t…
View article: Structural context of homomeric interactions in the lg domain of the MPZ (P0) myelin adhesion protein and relation to Charcot-Marie-Tooth disease phenotype variants
Structural context of homomeric interactions in the lg domain of the MPZ (P0) myelin adhesion protein and relation to Charcot-Marie-Tooth disease phenotype variants Open
Mutations in Myelin Protein Zero (MPZ) account for 5% of Charcot-Marie-Tooth cases and can cause demyelinating or axonal phenotypes, reflecting the diverse roles of MPZ in Schwann cells. MPZ holds the apposing membranes of the myelin sheat…
View article: Substrate Effects When Using Photons to Make Perovskite Solar Cells
Substrate Effects When Using Photons to Make Perovskite Solar Cells Open
To shorten annealing time and minimize substrate heating, flash lamps that deliver short, intense light pulses, commonly called photonic curing, are used to replace thermal annealing using hot plates or ovens. This method is different from…
View article: Dynein-Mediated Trafficking: A New Mechanism of Diabetic Podocytopathy
Dynein-Mediated Trafficking: A New Mechanism of Diabetic Podocytopathy Open
Key Points The expression of dynein is increased in human and rodent models of diabetic nephropathy (DN), eliciting a new dynein-driven pathogenesis. Uncontrolled dynein impairs the molecular sieve of kidney by remodeling the postendocytic…
View article: Silver Nanowire-Indium Zinc Oxide Composite Flexible Transparent Conducting Electrodes Made by Spin- coating and Photonic Curing
Silver Nanowire-Indium Zinc Oxide Composite Flexible Transparent Conducting Electrodes Made by Spin- coating and Photonic Curing Open
Realizing high-throughput, low-cost perovskite solar cell (PSC) manufacturing is highly sought-after in photovoltaic (PV) research in recent years. To fully achieve roll-to-roll (R2R) manufacturing of PSCs, it is important to consider the …
View article: Dynein-mediated trafficking and degradation of nephrin in diabetic podocytopathy
Dynein-mediated trafficking and degradation of nephrin in diabetic podocytopathy Open
Diabetic nephropathy (DN) is characterized by increased endocytosis and degradation of nephrin, a protein that comprises the molecular sieve of the glomerular filtration barrier, but the key trafficking mechanism that connects the initial …
View article: HD-PTP/PTPN23 hypomorphic mice display lipodystrophy
HD-PTP/PTPN23 hypomorphic mice display lipodystrophy Open
Endosomal Sorting Complexes Required for Transport (ESCRTs) drive reverse topology membrane remodeling events including the formation of intralumenal vesicles within multivesicular bodies, the budding of retroviruses from the plasma membra…
View article: Bro1 family proteins harmonize cargo sorting with vesicle formation
Bro1 family proteins harmonize cargo sorting with vesicle formation Open
The Endosomal Sorting Complexes Required for Transport (ESCRTs) drive membrane remodeling in a variety of cellular processes that include the formation of endosomal intralumenal vesicles (ILVs) during multivesicular body (MVB) biogenesis. …
View article: Selective endocytosis of Ca <sup>2+</sup> -permeable AMPARs by the Alzheimer’s disease risk factor CALM bidirectionally controls synaptic plasticity
Selective endocytosis of Ca <sup>2+</sup> -permeable AMPARs by the Alzheimer’s disease risk factor CALM bidirectionally controls synaptic plasticity Open
AMPA-type glutamate receptors (AMPARs) mediate fast excitatory neurotransmission, and the plastic modulation of their surface levels determines synaptic strength. AMPARs of different subunit compositions fulfill distinct roles in synaptic …
View article: Bro1 stimulates Vps4 to promote intralumenal vesicle formation during multivesicular body biogenesis
Bro1 stimulates Vps4 to promote intralumenal vesicle formation during multivesicular body biogenesis Open
Endosomal sorting complexes required for transport (ESCRT-0, -I, -II, -III) execute cargo sorting and intralumenal vesicle (ILV) formation during conversion of endosomes to multivesicular bodies (MVBs). The AAA-ATPase Vps4 regulates the ES…
View article: Mechanism of ubiquitin ligation and lysine prioritization by a HECT E3
Mechanism of ubiquitin ligation and lysine prioritization by a HECT E3 Open
Ubiquitination by HECT E3 enzymes regulates myriad processes, including tumor suppression, transcription, protein trafficking, and degradation. HECT E3s use a two-step mechanism to ligate ubiquitin to target proteins. The first step is gui…
View article: Functional Genomic Screening Independently Identifies CUL3 as a Mediator of Vemurafenib Resistance via Src-Rac1 Signaling Axis
Functional Genomic Screening Independently Identifies CUL3 as a Mediator of Vemurafenib Resistance via Src-Rac1 Signaling Axis Open
Patients with malignant melanoma have a 5-year survival rate of only 15-20% once the tumor has metastasized to distant tissues. While MAP kinase pathway inhibitors (MAPKi) are initially effective for the majority of patients with melanoma …