Robert E. Hill
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Bystander activation across a TAD boundary supports a cohesin-dependent transcription cluster model for enhancer function Open
Mammalian enhancers can regulate genes over large genomic distances, often skipping over other genes. Despite this, precise developmental regulation suggests that mechanisms exist to ensure enhancers only activate their correct targets. Sc…
<i>DGCR8</i> haploinsufficiency leads to primate-specific RNA dysregulation and pluripotency defects Open
The 22q11.2 deletion syndrome (22qDS) is a human disorder where the majority of clinical manifestations originate during embryonic development. 22qDS is caused by a microdeletion in one chromosome 22, including DGCR8, an essential gene for…
Bystander activation across a TAD boundary supports a cohesin-dependent hub-model for enhancer function Open
Enhancers in the mammalian genome are able to control their target genes over very large genomic distances, often across intervening genes. Yet the spatial and temporal specificity of developmental gene regulation would seem to demand that…
View article: UBR5 promotes antiviral immunity by disengaging the transcriptional brake on RIG-I like receptors
UBR5 promotes antiviral immunity by disengaging the transcriptional brake on RIG-I like receptors Open
The Retinoic acid-Inducible Gene I (RIG-I) like receptors (RLRs) are the major viral RNA sensors essential for the initiation of antiviral immune responses. RLRs are subjected to stringent transcriptional and posttranslational regulations,…
Cohesin is required for long-range enhancer action Open
Summary The mammalian genome is organised into topologically associating domains (TADs) that are formed through the process of cohesin-driven loop extrusion 1–3 and whose extent is constrained at TAD boundaries by orientation-dependent CTC…
Ubiquitin-protein ligase Ubr5 cooperates with hedgehog signalling to promote skeletal tissue homeostasis Open
Mammalian Hedgehog (HH) signalling pathway plays an essential role in tissue homeostasis and its deregulation is linked to rheumatological disorders. UBR5 is the mammalian homologue of the E3 ubiquitin-protein ligase Hyd, a negative regula…
A Highly Conserved Shh Enhancer Coordinates Hypothalamic and Craniofacial Development Open
Enhancers that are conserved deep in evolutionary time regulate characteristics held in common across taxonomic classes. Here, deletion of the highly conserved Shh enhancer SBE2 ( Shh brain enhancer 2) in mouse markedly reduced Shh express…
Ubiquitin-protein ligase<i>Ubr5</i>cooperates with Hedgehog signalling to promote skeletal tissue homeostasis Open
Mammalian Hedgehog (HH) signalling pathway plays an essential role in tissue homeostasis and its deregulation is linked to rheumatological disorders. UBR5 is the mammalian homologue of the E3 ubiquitin-protein ligase Hyd, a negative regula…
A Highly Conserved <i>Shh</i> Enhancer Coordinates Hypothalamic and Craniofacial Development Open
Enhancers that are conserved deep in evolutionary time regulate characteristics held in common across taxonomic classes. Here, deletion of the highly conserved Shh enhancer SBE2 ( Shh brain enhancer 2) markedly reduced Shh expression withi…
Developmentally regulated <i>Shh</i> expression is robust to TAD perturbations Open
Topologically associating domains (TADs) have been proposed to both guide and constrain enhancer activity. Shh is located within a TAD known to contain all its enhancers. To investigate the importance of chromatin conformation and TAD inte…
Developmentally regulated <i>Shh</i> expression is robust to TAD perturbations Open
Topologically Associating Domains (TADs) have been proposed to both guide and constrain enhancer activity. Shh is located within a TAD known to contain all its enhancers. To investigate the importance of chromatin conformation and TAD inte…
Does IEB make the grade? Alternative testing methods and educational outcomes: The case of the IEB in South Africa Open
According to the Independent Examinations Board (IEB, 2015), students who write the IEB National Senior Certificate school-leaving exam are at a distinct advantage and seem to be better prepared for the pressures and challenges faced durin…
A conditional Pax6 depletion study with no morphological effect on the adult mouse corneal epithelium Open
In a preliminary study, ubiquitous depletion of Pax6 by tamoxifen treatment of E9.5 CAG-CreERTg/-;Pax6fl/fl embryos affected eye development. Tamoxifen treatment of 12-week old, adult CAG-CreERTg/-;Pax6fl/+ and CAG-CreERTg/-;Pax6fl/fl mice…
MOESM1 of A conditional Pax6 depletion study with no morphological effect on the adult mouse corneal epithelium Open
Additional file 1. Fetal eye measurements.
Data from computer simulation of neutral drift among limbal epithelial stem cells of mosaic mice Open
It was previously shown that limbal epithelial stem cells (LESCs) around the periphery of the cornea maintain radial sectors of the corneal epithelium but that radial stripe numbers declined with age in mosaic mice. Originally, these resul…
Fibroblast growth factors (FGFs) prime the limb specific Shh enhancer for chromatin changes that balance histone acetylation mediated by E26 transformation-specific (ETS) factors Open
Sonic hedgehog (Shh) expression in the limb bud organizing centre called the zone of polarizing activity is regulated by the ZRS enhancer. Here, we examine in mouse and in a mouse limb-derived cell line the dynamic events that activate and…
Author response: Fibroblast growth factors (FGFs) prime the limb specific Shh enhancer for chromatin changes that balance histone acetylation mediated by E26 transformation-specific (ETS) factors Open
Article Figures and data Abstract eLife digest Introduction Results Discussion Materials and methods References Decision letter Author response Article and author information Metrics Abstract Sonic hedgehog (Shh) expression in the limb bud…
<i>Shh</i> and ZRS enhancer colocalisation is specific to the zone of polarising activity Open
Limb-specific Shh expression is regulated by the (∼1 Mb distant) ZRS enhancer. In the mouse, limb bud-restricted spatiotemporal Shh expression occurs from ∼E10 to E11.5 at the distal posterior margin and is essential for correct autopod fo…
Use of a Conditional Ubr5 Mutant Allele to Investigate the Role of an N-End Rule Ubiquitin-Protein Ligase in Hedgehog Signalling and Embryonic Limb Development Open
Hedgehog (Hh) signalling is a potent regulator of cell fate and function. While much is known about the events within a Hh-stimulated cell, far less is known about the regulation of Hh-ligand production. Drosophila Hyperplastic Discs (Hyd)…
<i>Shh</i> and ZRS enhancer co-localisation is specific to the zone of polarizing activity Open
Limb-specific Shh expression is regulated by the (~1 Mb distant) ZRS enhancer. In the mouse, limb bud restricted spatiotemporal expression of Shh occurs from ~E10-E11.5 at the distal posterior margin and is essential for correct autopod fo…