Robert H. Singer
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View article: Single-molecule imaging reveals activity-dependent regulation of <i>Camk2a</i> mRNAs at dendritic spines
Single-molecule imaging reveals activity-dependent regulation of <i>Camk2a</i> mRNAs at dendritic spines Open
Postsynaptic calcium/calmodulin-dependent protein kinase type II (CaMKII) integrates fleeting Ca 2+ transients into long-term synaptic potentiation (LTP). A persistent presence of CaMKIIα at dendritic spines during the maintenance of LTP f…
View article: Real-time imaging of transcriptional feedback in nonsense-mediated mRNA decay
Real-time imaging of transcriptional feedback in nonsense-mediated mRNA decay Open
Nonsense-mediated mRNA decay (NMD) is a translation-coupled mRNA decay pathway triggered by a premature termination codon (PTC). While in-frame stop codons are typically defined by cytoplasmic ribosomes, unexpected changes in transcription…
View article: Identification and classification of abundant RNA-binding proteins in the mouse lens and interactions of Carhsp1, Igf2bp1/ZBP1, and Ybx1 with crystallin and β-actin mRNAs
Identification and classification of abundant RNA-binding proteins in the mouse lens and interactions of Carhsp1, Igf2bp1/ZBP1, and Ybx1 with crystallin and β-actin mRNAs Open
RNA-binding proteins (RBPs) are critical regulators of mRNAs controlling all processes such as RNA transcription, transport, localization, translation, mRNA:ncRNA interactions, and decay. Cellular differentiation is driven by tissue-specif…
View article: Corrigendum: Inhibition of coronavirus HCoV-OC43 by targeting the eIF4F complex
Corrigendum: Inhibition of coronavirus HCoV-OC43 by targeting the eIF4F complex Open
[This corrects the article DOI: 10.3389/fphar.2022.1029093.].
View article: Single-molecule RNA-FISH analysis reveals stochasticity in reactivation of latent HIV-1 regulated by Nuclear Orphan Receptors NR4A and cMYC
Single-molecule RNA-FISH analysis reveals stochasticity in reactivation of latent HIV-1 regulated by Nuclear Orphan Receptors NR4A and cMYC Open
HIV-1 eradication strategies require complete reactivation of HIV-1 latent cells by Latency Reversing Agents (LRA). Current methods lack effectiveness due to incomplete proviral reactivation. We employed a single-molecule RNA-FISH (smRNA-F…
View article: Transcriptome-wide mRNP condensation precedes stress granule formation and excludes new mRNAs
Transcriptome-wide mRNP condensation precedes stress granule formation and excludes new mRNAs Open
Stress-induced mRNP condensation is conserved across eukaryotes, resulting in stress granule formation under intense stresses, yet the mRNA composition and function of these condensates remain unclear. Exposure of ribosome-free mRNA follow…
View article: A series of spontaneously blinking dyes for super-resolution microscopy
A series of spontaneously blinking dyes for super-resolution microscopy Open
Spontaneously blinking fluorophores permit the detection and localization of individual molecules without reducing buffers or caging groups, thus simplifying single-molecule localization microscopy (SMLM). The intrinsic blinking properties…
View article: Visualization of Early RNA Replication Kinetics of SARS-CoV-2 by Using Single Molecule RNA-FISH Combined with Immunofluorescence
Visualization of Early RNA Replication Kinetics of SARS-CoV-2 by Using Single Molecule RNA-FISH Combined with Immunofluorescence Open
SARS-CoV-2 infection remains a global burden. Despite intensive research, the mechanism and dynamics of early viral replication are not completely understood, such as the kinetics of the formation of genomic RNA (gRNA), sub-genomic RNA (sg…
View article: Visualization of Early RNA Replication Kinetics of SARS-CoV-2 by Using Single Molecule RNA-Fish Combined with Immunofluorescence
Visualization of Early RNA Replication Kinetics of SARS-CoV-2 by Using Single Molecule RNA-Fish Combined with Immunofluorescence Open
SARS-CoV-2 infection remains a global burden. Despite intensive research, the mechanism and dynamics of early viral replication are not completely understood including the kinetics of formation of genomic RNA (gRNA), sub-genomic RNA (sgRNA…
View article: Lysosomal release of amino acids at ER three-way junctions regulates transmembrane and secretory protein mRNA translation
Lysosomal release of amino acids at ER three-way junctions regulates transmembrane and secretory protein mRNA translation Open
One-third of the mammalian proteome is comprised of transmembrane and secretory proteins that are synthesized on endoplasmic reticulum (ER). Here, we investigate the spatial distribution and regulation of mRNAs encoding these membrane and …
View article: Frontmatter
Frontmatter Open
ReFocus is a series of contemporary methodological and theoretical approaches to the interdisciplinary analyses and interpretations of international film directors, from the celebrated to the ignored, in direct relationship to their respec…
View article: Global analysis of contact-dependent human-to-mouse intercellular mRNA and lncRNA transfer in cell culture
Global analysis of contact-dependent human-to-mouse intercellular mRNA and lncRNA transfer in cell culture Open
Full-length mRNAs transfer between adjacent mammalian cells via direct cell-to-cell connections called tunneling nanotubes (TNTs). However, the extent of mRNA transfer at the transcriptome-wide level (the ‘transferome’) is unknown. Here, w…
View article: mRNA Localization and Local Translation of the Microtubule Severing Enzyme, Fidgetin-Like 2, in Polarization, Migration and Outgrowth
mRNA Localization and Local Translation of the Microtubule Severing Enzyme, Fidgetin-Like 2, in Polarization, Migration and Outgrowth Open
SUMMARY Cell motility requires strict spatiotemporal control of protein expression. During cell migration, mRNA localization and local translation in subcellular areas like the leading edge and protrusions are particularly advantageous for…
View article: Supplementary Figure 3 from Altered Dynamics of Intestinal Cell Maturation in <i>Apc<sup>1638N/+</sup></i> Mice
Supplementary Figure 3 from Altered Dynamics of Intestinal Cell Maturation in <i>Apc<sup>1638N/+</sup></i> Mice Open
Supplementary Figure 3 from Altered Dynamics of Intestinal Cell Maturation in Apc1638N/+ Mice
View article: Supplementary Table 4 from Identification and Testing of a Gene Expression Signature of Invasive Carcinoma Cells within Primary Mammary Tumors
Supplementary Table 4 from Identification and Testing of a Gene Expression Signature of Invasive Carcinoma Cells within Primary Mammary Tumors Open
Supplementary Table 4 from Identification and Testing of a Gene Expression Signature of Invasive Carcinoma Cells within Primary Mammary Tumors
View article: Supplementary Table 3 from Identification and Testing of a Gene Expression Signature of Invasive Carcinoma Cells within Primary Mammary Tumors
Supplementary Table 3 from Identification and Testing of a Gene Expression Signature of Invasive Carcinoma Cells within Primary Mammary Tumors Open
Supplementary Table 3 from Identification and Testing of a Gene Expression Signature of Invasive Carcinoma Cells within Primary Mammary Tumors
View article: Supplementary Table 3 from Altered Dynamics of Intestinal Cell Maturation in <i>Apc<sup>1638N/+</sup></i> Mice
Supplementary Table 3 from Altered Dynamics of Intestinal Cell Maturation in <i>Apc<sup>1638N/+</sup></i> Mice Open
Supplementary Table 3 from Altered Dynamics of Intestinal Cell Maturation in Apc1638N/+ Mice
View article: Supplementary Table 1 from Identification and Testing of a Gene Expression Signature of Invasive Carcinoma Cells within Primary Mammary Tumors
Supplementary Table 1 from Identification and Testing of a Gene Expression Signature of Invasive Carcinoma Cells within Primary Mammary Tumors Open
Supplementary Table 1 from Identification and Testing of a Gene Expression Signature of Invasive Carcinoma Cells within Primary Mammary Tumors
View article: Supplementary Table 3 from Altered Dynamics of Intestinal Cell Maturation in <i>Apc<sup>1638N/+</sup></i> Mice
Supplementary Table 3 from Altered Dynamics of Intestinal Cell Maturation in <i>Apc<sup>1638N/+</sup></i> Mice Open
Supplementary Table 3 from Altered Dynamics of Intestinal Cell Maturation in Apc1638N/+ Mice
View article: Supplementary Figure 2 from Altered Dynamics of Intestinal Cell Maturation in <i>Apc<sup>1638N/+</sup></i> Mice
Supplementary Figure 2 from Altered Dynamics of Intestinal Cell Maturation in <i>Apc<sup>1638N/+</sup></i> Mice Open
Supplementary Figure 2 from Altered Dynamics of Intestinal Cell Maturation in Apc1638N/+ Mice
View article: Supplementary Figure 1 from Altered Dynamics of Intestinal Cell Maturation in <i>Apc<sup>1638N/+</sup></i> Mice
Supplementary Figure 1 from Altered Dynamics of Intestinal Cell Maturation in <i>Apc<sup>1638N/+</sup></i> Mice Open
Supplementary Figure 1 from Altered Dynamics of Intestinal Cell Maturation in Apc1638N/+ Mice
View article: Data from Identification and Testing of a Gene Expression Signature of Invasive Carcinoma Cells within Primary Mammary Tumors
Data from Identification and Testing of a Gene Expression Signature of Invasive Carcinoma Cells within Primary Mammary Tumors Open
We subjected cells collected using an in vivo invasion assay to cDNA microarray analysis to identify the gene expression profile of invasive carcinoma cells in primary mammary tumors. Expression of genes involved in cell division, survival…
View article: Supplementary Table 3 from Identification and Testing of a Gene Expression Signature of Invasive Carcinoma Cells within Primary Mammary Tumors
Supplementary Table 3 from Identification and Testing of a Gene Expression Signature of Invasive Carcinoma Cells within Primary Mammary Tumors Open
Supplementary Table 3 from Identification and Testing of a Gene Expression Signature of Invasive Carcinoma Cells within Primary Mammary Tumors
View article: Supplementary Table 4 from Identification and Testing of a Gene Expression Signature of Invasive Carcinoma Cells within Primary Mammary Tumors
Supplementary Table 4 from Identification and Testing of a Gene Expression Signature of Invasive Carcinoma Cells within Primary Mammary Tumors Open
Supplementary Table 4 from Identification and Testing of a Gene Expression Signature of Invasive Carcinoma Cells within Primary Mammary Tumors