Robert Morbitzer
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View article: Targeted knockout of a conserved plant mitochondrial gene by genome editing
Targeted knockout of a conserved plant mitochondrial gene by genome editing Open
Fusion proteins derived from transcription activator-like effectors (TALEs) have emerged as genome editing tools for mitochondria. TALE nucleases (TALENs) have been applied to delete chimaeric reading frames and duplicated (redundant) gene…
View article: The eINTACT system dissects bacterial exploitation of plant osmosignalling to enhance virulence
The eINTACT system dissects bacterial exploitation of plant osmosignalling to enhance virulence Open
Bacteria inject effector proteins into host cells to manipulate cellular processes that promote disease. Since bacteria deliver minuscule amounts of effectors only into targeted host cells, it is technically challenging to capture effector…
View article: The tomato resistance gene <i>Bs4</i> suppresses leaf watersoaking phenotypes induced by <scp>AvrHah1</scp>, a transcription activator‐like effector from tomato‐pathogenic xanthomonads
The tomato resistance gene <i>Bs4</i> suppresses leaf watersoaking phenotypes induced by <span>AvrHah1</span>, a transcription activator‐like effector from tomato‐pathogenic xanthomonads Open
Summary The Xanthomonas transcription activator‐like effector (TALE) protein AvrBs3 transcriptionally activates the executor‐type resistance ( R ) gene Bs3 from pepper ( Capsicum annuum ), thereby triggering a hypersensitive cell death rea…
View article: Efficient <scp>TALEN</scp>‐mediated gene editing in wheat
Efficient <span>TALEN</span>‐mediated gene editing in wheat Open
Plant genome editing is a major advance in the production of novel plant genotypes. There is, however, only a single previous report of applying this technology to hexaploid wheat using TALEN-mediated gene editing to produce heritable modi…
View article: TALE-induced bHLH transcription factors that activate a pectate lyase contribute to water soaking in bacterial spot of tomato
TALE-induced bHLH transcription factors that activate a pectate lyase contribute to water soaking in bacterial spot of tomato Open
Significance AvrHah1 [avirulence (avr) gene homologous to avrBs3 and hax2, no. 1] is a transcription activator-like (TAL) effector (TALE) in Xanthomonas gardneri that enhances water soaking in its known hosts tomato, pepper, and Nicotiana …
View article: Comparison of gene activation by two TAL effectors from <scp> <i>X</i> </scp> <i>anthomonas axonopodis</i> pv. <i>manihotis</i> reveals candidate host susceptibility genes in cassava
Comparison of gene activation by two TAL effectors from <span> <i>X</i> </span> <i>anthomonas axonopodis</i> pv. <i>manihotis</i> reveals candidate host susceptibility genes in cassava Open
Summary Xanthomonas axonopodis pv. manihotis ( Xam ) employs transcription activator‐like (TAL) effectors to promote bacterial growth and symptom formation during infection of cassava. TAL effectors are secreted via the bacterial type III …
View article: TRANSCRIPTION ACTIVATOR-LIKE EFFECTOR NUCLEASE-Mediated Generation and Metabolic Analysis of Camalexin-Deficient <i>cyp71a12 cyp71a13</i> Double Knockout Lines
TRANSCRIPTION ACTIVATOR-LIKE EFFECTOR NUCLEASE-Mediated Generation and Metabolic Analysis of Camalexin-Deficient <i>cyp71a12 cyp71a13</i> Double Knockout Lines Open
In Arabidopsis (Arabidopsis thaliana), a number of defense-related metabolites are synthesized via indole-3-acetonitrile (IAN), including camalexin and indole-3-carboxylic acid (ICOOH) derivatives. Cytochrome P450 71A13 (CYP71A13) is a key…