Robert L. Moritz
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Integrative Multi-Omic Signatures of Longevity and Healthy Aging Open
There is a growing interest in serum-based biomarkers that reveal the mechanisms of healthy aging. The Longevity Consortium generated untargeted mass spectrometry-based proteomic and metabolomic datasets from serum samples across four coho…
View article: mzPeak: Designing a Scalable, Interoperable, and Future-Ready Mass Spectrometry Data Format
mzPeak: Designing a Scalable, Interoperable, and Future-Ready Mass Spectrometry Data Format Open
Advances in mass spectrometry (MS) instrumentation, including higher resolution, faster scan speeds, and improved sensitivity, have dramatically increased the data volume and complexity. The adoption of imaging and ion mobility further amp…
UBR-1 enzyme network regulates glutamate homeostasis to affect organismal behavior and developmental viability Open
Johanson-Blizzard Syndrome (JBS) is an autosomal recessive spectrum disorder associated with the UBR-1 ubiquitin ligase that features developmental delay including motor abnormalities. Here, we demonstrate that C. elegans UBR-1 regulates h…
View article: An expanded reference catalog of translated open reading frames for biomedical research
An expanded reference catalog of translated open reading frames for biomedical research Open
Non-canonical (i.e., unannotated) open reading frames (ncORFs) have until recently been omitted from reference genome annotations, despite evidence of their translation, limiting their incorporation into biomedical research. To address thi…
View article: Assessment of Reported Error Rates in Crosslinking Mass Spectrometry
Assessment of Reported Error Rates in Crosslinking Mass Spectrometry Open
We benchmarked eight software tools in crosslinking mass spectrometry (MS) to evaluate their accuracy in detecting protein-protein interactions. Whereas about half performed reliably, others reported up to 31-fold more false interactions t…
<i>Candida albicans</i> : A Comprehensive View of the Proteome Open
We describe a new release of the Candida albicans PeptideAtlas proteomics spectral resource (build 2024-03), providing a sequence coverage of 79.5% at the canonical protein level, matched mass spectrometry spectra, and experimental evidenc…
View article: Limelight: An Open, Web-Based Tool for Visualizing, Sharing, and Analyzing Mass Spectrometry Data from DDA Pipelines
Limelight: An Open, Web-Based Tool for Visualizing, Sharing, and Analyzing Mass Spectrometry Data from DDA Pipelines Open
Liquid chromatography-tandem mass spectrometry employing data-dependent acquisition (DDA) is a mature, widely used proteomics technique routinely applied to proteome profiling, protein-protein interaction studies, biomarker discovery, and …
Detection of human unannotated microproteins by mass spectrometry-based proteomics: a community assessment Open
Thousands of short open reading frames (sORFs) are translated outside of annotated coding sequences. Recent studies have pioneered searching for sORF-encoded microproteins in mass spectrometry (MS)- based proteomics and peptidomics dataset…
View article: Widespread release of translational repression across Plasmodium’s host-to-vector transmission event
Widespread release of translational repression across Plasmodium’s host-to-vector transmission event Open
Malaria parasites must respond quickly to environmental changes, including during their transmission between mammalian and mosquito hosts. Therefore, female gametocytes proactively produce and translationally repress mRNAs that encode esse…
<i>Candida albicans</i> a comprehensive view of the proteome Open
We describe a new release of the Candida albicans PeptideAtlas proteomics spectral resource (build 2024-03), providing a sequence coverage of 79.5% at the canonical protein level, matched mass spectrometry spectra, and experimental evidenc…
BLOOD MULTIOMIC PROFILES REFLECT SYSTEM STATES OF ORGANS IN MICE Open
The rate of aging can vary among organs, as evidenced by organ-specific biological age models. Such models are typically constructed based on the blood biomarkers or phenotypes that are known to be specific to a particular organ. However, …
The 2024 Report on the Human Proteome from the HUPO Human Proteome Project Open
The Human Proteome Project (HPP), the flagship initiative of the Human Proteome Organization (HUPO), has pursued two goals: (1) to credibly identify at least one isoform of every protein-coding gene and (2) to make proteomics an integral p…
View article: Limelight - An open, web-based tool for visualizing, sharing, and analyzing mass spectrometry data from DDA pipelines
Limelight - An open, web-based tool for visualizing, sharing, and analyzing mass spectrometry data from DDA pipelines Open
Liquid chromatography-tandem mass spectrometry employing data-dependent acquisition (DDA) is a mature, widely used proteomics technique routinely applied to proteome profiling, protein-protein interaction studies, biomarker discovery, and …
The Circulating Proteome─Technological Developments, Current Challenges, and Future Trends Open
Recent improvements in proteomics technologies have fundamentally altered our capacities to characterize human biology. There is an ever-growing interest in using these novel methods for studying the circulating proteome, as blood offers a…
View article: Detection and Quantification of Drug–Protein Adducts in Human Liver
Detection and Quantification of Drug–Protein Adducts in Human Liver Open
Covalent protein adducts formed by drugs or their reactive metabolites are risk factors for adverse reactions, and inactivation of cytochrome P450 (CYP) enzymes. Characterization of drug-protein adducts is limited due to lack of methods id…
View article: High-quality peptide evidence for annotating non-canonical open reading frames as human proteins
High-quality peptide evidence for annotating non-canonical open reading frames as human proteins Open
A major scientific drive is to characterize the protein-coding genome as it provides the primary basis for the study of human health. But the fundamental question remains: what has been missed in prior genomic analyses? Over the past decad…
The major surface protein of malaria sporozoites is GPI-anchored to the plasma membrane Open
Glycosylphosphatidylinositol (GPI) anchor protein modification in Plasmodium species is well known and represents the principal form of glycosylation in these organisms. The structure and biosynthesis of GPI anchors of Plasmodium spp. has …
View article: Comprehensive Overview of Bottom-Up Proteomics Using Mass Spectrometry
Comprehensive Overview of Bottom-Up Proteomics Using Mass Spectrometry Open
Proteomics is the large scale study of protein structure and function from biological systems through protein identification and quantification. "Shotgun proteomics" or "bottom-up proteomics" is the prevailing strategy, in which proteins a…
View article: The major surface protein of malaria sporozoites is GPI-anchored to the plasma membrane
The major surface protein of malaria sporozoites is GPI-anchored to the plasma membrane Open
Glycosylphosphatidylinositol (GPI) anchor protein modification in Plasmodium species is well known and represents the principal form of glycosylation in these organisms. The structure and biosynthesis of GPI anchors of Plasmodium spp. has …
View article: Global Release of Translational Repression Across <i>Plasmodium’s</i> Host-to-Vector Transmission Event
Global Release of Translational Repression Across <i>Plasmodium’s</i> Host-to-Vector Transmission Event Open
Malaria parasites must be able to respond quickly to changes in their environment, including during their transmission between mammalian hosts and mosquito vectors. Therefore, before transmission, female gametocytes proactively produce and…
The 2023 Report on the Proteome from the HUPO Human Proteome Project Open
Since 2010, the Human Proteome Project (HPP), the flagship initiative of the Human Proteome Organization (HUPO), has pursued two goals: (1) to credibly identify the protein parts list and (2) to make proteomics an integral part of multiomi…
MULTIOMIC SYSTEMS ANALYSIS OF LIFESPAN-EXTENDING INTERVENTIONS IN MOUSE TISSUES Open
Aging is associated with dysregulation of molecular, cellular, and physiological processes. Nutritional and pharmacological interventions with different postulated modes of action have been shown to extend lifespan and delay aging-related …
PROSPECTIVE MULTI-OMIC ANALYSIS OF HUMAN LONGEVITY COHORTS IDENTIFIES ANALYTE NETWORKS ASSOCIATED WITH LONGEVITY Open
Serum based biomarkers of longevity have long been sought to explain the mechanisms of healthy aging and longevity. Using a 1:3 case cohort design, the Longevity Consortium has produced untargeted mass spectrometry based proteomic and meta…
View article: Comprehensive Overview of Bottom-up Proteomics using Mass Spectrometry
Comprehensive Overview of Bottom-up Proteomics using Mass Spectrometry Open
Proteomics is the large scale study of protein structure and function from biological systems through protein identification and quantification. "Shotgun proteomics" or "bottom-up proteomics" is the prevailing strategy, in which proteins a…