Robert P. Mortlock
YOU?
Author Swipe
View article: Molecular cloning of the Escherichia coli B L-fucose-D-arabinose gene cluster
Molecular cloning of the Escherichia coli B L-fucose-D-arabinose gene cluster Open
To metabolize the uncommon pentose D-arabinose, enteric bacteria often recruit the enzymes of the L-fucose pathway by a regulatory mutation. However, Escherichia coli B can grow on D-arabinose without the requirement of a mutation, using s…
View article: Isolation and characterization of Escherichia coli mutants able to utilize the novel pentose L-ribose
Isolation and characterization of Escherichia coli mutants able to utilize the novel pentose L-ribose Open
Wild-type strains of Escherichia coli were unable to utilize L-ribose for growth. However, L-ribose-positive mutants could be isolated from strains of E. coli K-12 which contained a ribitol operon. L-ribose-positive strains of E. coli, iso…
View article: D-arabinose metabolism in Escherichia coli B: induction and cotransductional mapping of the L-fucose-D-arabinose pathway enzymes
D-arabinose metabolism in Escherichia coli B: induction and cotransductional mapping of the L-fucose-D-arabinose pathway enzymes Open
D-Arabinose is degraded by Escherichia coli B via some of the L-fucose pathway enzymes and a D-ribulokinase which is distinct from the L-fuculokinase of the L-fucose pathway. We found that L-fucose and D-arabinose acted as the apparent ind…
View article: Isolation of a mutation resulting in constitutive synthesis of L-fucose catabolic enzymes
Isolation of a mutation resulting in constitutive synthesis of L-fucose catabolic enzymes Open
A ribitol-positive transductant of Escherichia coli K-12, JM2112, was used to facilitate the isolation and identification of mutations affecting the L-fucose catabolic pathway. Analysis of L-fucose-negative mutants of JM2112 enabled us to …
View article: Construction of an improved D-arabinose pathway in Escherichia coli K-12
Construction of an improved D-arabinose pathway in Escherichia coli K-12 Open
A ribitol catabolic pathway was transduced into Escherichia coli K-12 in an effort to determine whether the ribitol pathway would confer an advantage to D-arabinose-positive mutants growing on D-arabinose as the sole carbon source. Competi…
View article: Inducible xylitol dehydrogenases in enteric bacteria
Inducible xylitol dehydrogenases in enteric bacteria Open
Morganella morganii ATCC 25829, Providencia stuartii ATCC 25827, Serratia marcescens ATCC 13880, and Erwinia sp. strain 4D2P were found to induce a xylitol dehydrogenase when grown on a xylitol-containing medium. The xylitol dehydrogenases…
View article: Characterization of xylitol-utilizing mutants of Erwinia uredovora
Characterization of xylitol-utilizing mutants of Erwinia uredovora Open
Of the four pentitols ribitol, xylitol, D-arabitol, and L-arabitol, Erwinia uredovora was able to utilize only D-arabitol as a carbon and energy source. Although attempts to isolate ribitol- or L-arabitol-utilizing mutants were unsuccessfu…
View article: Production of D- and L-xylulose by mutants of Klebsiella pneumoniae and Erwinia uredovora
Production of D- and L-xylulose by mutants of Klebsiella pneumoniae and Erwinia uredovora Open
D-Xylulose and L-xylulose were produced biologically by the oxidation of a corresponding pentitol. A Klebsiella pneumoniae mutant was constructed for the oxidation of D-arabitol to D-xylulose. This mutant constitutively synthesized the D-a…
View article: Directed evolution of a second xylitol catabolic pathway in Klebsiella pneumoniae
Directed evolution of a second xylitol catabolic pathway in Klebsiella pneumoniae Open
Klebsiella pneumoniae PRL-R3 has inducible catabolic pathways for the degradation of ribitol and D-arabitol but cannot utilize xylitol as a growth substrate. A mutation in the rbtB regulatory gene of the ribitol operon permits the constitu…
View article: Biosynthesis and catabolism of 6-deoxy L-talitol by Klebsiella aerogenes mutants
Biosynthesis and catabolism of 6-deoxy L-talitol by Klebsiella aerogenes mutants Open
A mutant strain of Klebsiella aerogenes was constructed and, when incubated anaerobically with L-fucose and glycerol, synthesized and excreted a novel methyl pentitol, 6-deoxy L-talitol. The mutant was constitutive for the synthesis of L-f…
View article: Utilization of D-xylose by wild-type strains of Salmonella typhimurium
Utilization of D-xylose by wild-type strains of Salmonella typhimurium Open
Enzyme studies of strains of Salmonella typhimurium representing biotypes that utilized D-xylose rapidly (xylose strong) or slowly (xylose weak) showed that they were different in the utilization of D-xylose because the xylose-weak strains…
View article: Natural and altered induction of the L-fucose catabolic enzymes in Klebsiella aerogenes
Natural and altered induction of the L-fucose catabolic enzymes in Klebsiella aerogenes Open
Mutants of Klebsiella aerogenes W70 were isolated that had gained the ability to utilize the uncommon pentose D-arabinose as their sole source of carbon and energy. In contrast to the D-arabinose-negative, parent strain, these mutants were…
View article: <scp>d</scp> -Arabitol Catabolic Pathway in <i>Klebsiella aerogenes</i>
<span>d</span> -Arabitol Catabolic Pathway in <i>Klebsiella aerogenes</i> Open
Klebsiella aerogenes strain W70 has an inducible pathway for the degradation of d -arabitol which is comparable to the one found in Aerobacter aerogenes strain PRL-R3. The pathway is also similar to the pathway of ribitol catabolism in tha…
View article: Ribitol Catabolic Pathway in <i>Klebsiella aerogenes</i>
Ribitol Catabolic Pathway in <i>Klebsiella aerogenes</i> Open
In Klebsiella aerogenes W70, there is an inducible pathway for the catabolism of ribitol consisting of at least two enzymes, ribitol dehydrogenase (RDH) and d -ribulokinase (DRK). These two enzymes are coordinately controlled and induced i…
View article: Close Genetic Linkage of the Determinants of the Ribitol and <scp>d</scp> -Arabitol Catabolic Pathways in <i>Klebsiella aerogenes</i>
Close Genetic Linkage of the Determinants of the Ribitol and <span>d</span> -Arabitol Catabolic Pathways in <i>Klebsiella aerogenes</i> Open
Klebsiella aerogenes strain W70 has separate inducible pathways for the degradation of the pentitols ribitol and d -arabitol. These pathways are closely linked genetically as determined by transduction with phage PW52. There are two regula…
View article: Regulation of <scp>d</scp> -Xylose and <scp>d</scp> -Arabitol Catabolism by <i>Aerobacter aerogenes</i>
Regulation of <span>d</span> -Xylose and <span>d</span> -Arabitol Catabolism by <i>Aerobacter aerogenes</i> Open
Aerobacter aerogenes strain PRL-R3 possesses inducible enzyme pathways for the catabolism of d -xylose and d -arabitol. d -Xylose is the apparent inducer for d -xylose isomerase and d -xylulokinase. d -Arabitol is the apparent inducer for …
View article: Metabolism of <scp>d</scp> -Arabinose by <i>Aerobacter aerogenes</i> : Purification of the Isomerase <sup> <i>a</i> </sup>
Metabolism of <span>d</span> -Arabinose by <i>Aerobacter aerogenes</i> : Purification of the Isomerase <sup> <i>a</i> </sup> Open
In Aerobacter aerogenes , the mutational event permitting the utilization of d -arabinose as a source of carbon and energy is a regulatory mutation resulting in the constitutive synthesis of certain enzymes of the l -fucose catabolic pathw…
View article: Growth of <i>Aerobacter aerogenes</i> on <scp>d</scp> -Arabinose: Origin of the Enzyme Activities
Growth of <i>Aerobacter aerogenes</i> on <span>d</span> -Arabinose: Origin of the Enzyme Activities Open
Mutants of Aerobacter aerogenes can be selected which are capable of utilizing d -arabinose as a sole source of carbon and energy for growth. Mutants can also be selected which are capable of using l -xylose. The mutational event permittin…
View article: Metabolism of <scp>d</scp> -Arabinose: a New Pathway in <i>Escherichia coli</i>
Metabolism of <span>d</span> -Arabinose: a New Pathway in <i>Escherichia coli</i> Open
Several growth characteristics of Escherichia coli K-12 suggest that growth on l -fucose results in the synthesis of all the enzymes necessary for growth on d -arabinose. Conversely, when a mutant of E. coli is grown on d -arabinose, all o…
View article: Metabolism of <scp>d</scp> -Arabinose: Origin of a <scp>d</scp> -Ribulokinase Activity in <i>Escherichia coli</i>
Metabolism of <span>d</span> -Arabinose: Origin of a <span>d</span> -Ribulokinase Activity in <i>Escherichia coli</i> Open
The kinase responsible for the phosphorylation of d -ribulose was purified 45.5-fold from a strain of Escherichia coli K-12 capable of growth on d -arabinose with no separation of d -ribulo- or l -fuculokinase activities. Throughout the pu…
View article: Requirement for Coenzyme A in the Phosphoroclastic Reaction of Anaerobic Bacteria
Requirement for Coenzyme A in the Phosphoroclastic Reaction of Anaerobic Bacteria Open
Various bacteria which degrade pyruvate by the phosphoroclastic reaction were examined with respect to the role of coenzyme A (CoA) in this reaction. The strictly anaerobic bacteria, which cleave pyruvate by the phosphoroclastic reaction c…
View article: Regulation of Pentitol Metabolism by <i>Aerobacter aerogenes</i> I. Coordinate Control of Ribitol Dehydrogenase and <scp>d</scp> -Ribulokinase Activities
Regulation of Pentitol Metabolism by <i>Aerobacter aerogenes</i> I. Coordinate Control of Ribitol Dehydrogenase and <span>d</span> -Ribulokinase Activities Open
Induction studies on Aerobacter aerogenes strain PRL-R3, using ribitol as the inducer-substrate, indicated that two enzymes of ribitol catabolism, ribitol dehydrogenase and d -ribulokinase, are coordinately induced. The utilization of d -a…
View article: Regulation of Pentitol Metabolism by <i>Aerobacter aerogenes</i> II. Induction of the Ribitol Pathway
Regulation of Pentitol Metabolism by <i>Aerobacter aerogenes</i> II. Induction of the Ribitol Pathway Open
The incubation of Aerobacter aerogenes PRL-R3 with ribitol resulted in the induction of ribitol dehydrogenase and d -ribulokinase, coordinately controlled enzymes of the pathway of ribitol catabolism. A dehydrogenase-negative mutant was un…
View article: A basis for utlization of unnatural pentoses and pentitols by Aerobacter aerogenes.
A basis for utlization of unnatural pentoses and pentitols by Aerobacter aerogenes. Open
The present research uncovers the dynamic nature of student discipline. The findings show that discipline escalates during the school year, that discipline escalates more severely for Black students, and that racial disparities in discipli…
View article: Metabolism of Pentoses and Pentitols by <i>Aerobacter aerogenes</i> III. Physical and Immunological Properties of Pentitol Dehydrogenases and Pentulokinases
Metabolism of Pentoses and Pentitols by <i>Aerobacter aerogenes</i> III. Physical and Immunological Properties of Pentitol Dehydrogenases and Pentulokinases Open
Mortlock , R. P. (Michigan State University, East Lansing), D. D. Fossitt, D. H. Petering, and W. A. Wood . Metabolism of pentoses and pentitols by Aerobacter aerogenes . III. Physical and immunological properties of pentitol dehydrogenase…
View article: METABOLISM OF PENTOSES AND PENTITOLS BY <i>AEROBACTER AEROGENES</i> II
METABOLISM OF PENTOSES AND PENTITOLS BY <i>AEROBACTER AEROGENES</i> II Open
Mortlock , R. P. (Michigan State University, East Lansing), and W. A. Wood . Metabolism of pentoses by Aerobacter aerogenes . II. Mechanism of acquisition of kinase, isomerase, and dehydrogenase activity. J. Bacteriol. 88: 845–849. 1964.— …
View article: METABOLISM OF PENTOSES AND PENTITOLS BY <i>AEROBACTER AEROGENES</i> I
METABOLISM OF PENTOSES AND PENTITOLS BY <i>AEROBACTER AEROGENES</i> I Open
Mortlock , R. P. (Michigan State University, East Lansing) and W. A. Wood . Metabolism of pentoses and pentitols by Aerobacter aerogenes . I. Demonstration of pentose isomerase, pentulokinase, and pentitol dehydrogenase enzyme families. J.…