Robert Porteous
YOU?
Author Swipe
Low dose of propyl-pyrazole-triol, an agonist of estrogen receptor alpha, administration stimulates the Coolidge effect in fadrozole-treated male rats Open
Estrogen receptor (ER) α is involved in male sexual function. Here, we aim to investigate how ERα activation influences sexual satiety and the Coolidge effect (i.e., when a rat, that has reached sexual satiety, experiences an increased aro…
CRISPR-Cas9 knockdown of ESR1 in preoptic GABA-kisspeptin neurons suppresses the preovulatory surge and estrous cycles in female mice Open
Evidence suggests that estradiol-sensing preoptic area GABA neurons are involved in the preovulatory surge mechanism necessary for ovulation. In vivo CRISPR-Cas9 editing was used to achieve a 60–70% knockdown in estrogen receptor alpha (ES…
Author Response: CRISPR-Cas9 knockdown of ESR1 in preoptic GABA-kisspeptin neurons suppresses the preovulatory surge and estrous cycles in female mice Open
Evidence suggests that estradiol-sensing preoptic area GABA neurons are involved in the pre-ovulatory surge mechanism necessary for ovulation. In vivo CRISPR-Cas9 editing was used to achieve a 60-70% knockdown in estrogen receptor alpha (E…
CRISPR-Cas9 knockdown of ESR1 in preoptic GABA-kisspeptin neurons suppresses the preovulatory surge and estrous cycles in female mice Open
Evidence suggests that estradiol-sensing preoptic area GABA neurons are involved in the pre-ovulatory surge mechanism necessary for ovulation. In vivo CRISPR-Cas9 editing was used to achieve a 60-70% knockdown in estrogen receptor alpha (E…
Reviewer #3 (Public Review): CRISPR-Cas9 knockdown of ESR1 in preoptic GABA-kisspeptin neurons suppresses the preovulatory surge and estrous cycles in female mice Open
Evidence suggests that estradiol-sensing preoptic area GABA neurons are involved in the pre-ovulatory surge mechanism necessary for ovulation. In vivo CRISPR-Cas9 editing was used to achieve a 60-70% knockdown in estrogen receptor alpha (E…
Reviewer #2 (Public Review): CRISPR-Cas9 knockdown of ESR1 in preoptic GABA-kisspeptin neurons suppresses the preovulatory surge and estrous cycles in female mice Open
Evidence suggests that estradiol-sensing preoptic area GABA neurons are involved in the pre-ovulatory surge mechanism necessary for ovulation. In vivo CRISPR-Cas9 editing was used to achieve a 60-70% knockdown in estrogen receptor alpha (E…
Reviewer #1 (Public Review): CRISPR-Cas9 knockdown of ESR1 in preoptic GABA-kisspeptin neurons suppresses the preovulatory surge and estrous cycles in female mice Open
Evidence suggests that estradiol-sensing preoptic area GABA neurons are involved in the pre-ovulatory surge mechanism necessary for ovulation. In vivo CRISPR-Cas9 editing was used to achieve a 60-70% knockdown in estrogen receptor alpha (E…
Reviewer #2 (Public Review): CRISPR-Cas9 knockdown of ESR1 in preoptic GABA-kisspeptin neurons abolishes the preovulatory surge and estrous cycles in female mice Open
Evidence suggests that estradiol-sensing preoptic area GABA neurons are involved in the pre-ovulatory surge mechanism necessary for ovulation. In vivo CRISPR-Cas9 editing was used to achieve a 60-70% knockdown in estrogen receptor alpha (E…
Reviewer #1 (Public Review): CRISPR-Cas9 knockdown of ESR1 in preoptic GABA-kisspeptin neurons abolishes the preovulatory surge and estrous cycles in female mice Open
Evidence suggests that estradiol-sensing preoptic area GABA neurons are involved in the pre-ovulatory surge mechanism necessary for ovulation. In vivo CRISPR-Cas9 editing was used to achieve a 60-70% knockdown in estrogen receptor alpha (E…
CRISPR-Cas9 knockdown of ESR1 in preoptic GABA-kisspeptin neurons abolishes the preovulatory surge and estrous cycles in female mice Open
Evidence suggests that estradiol-sensing preoptic area GABA neurons are involved in the pre-ovulatory surge mechanism necessary for ovulation. In vivo CRISPR-Cas9 editing was used to achieve a 60-70% knockdown in estrogen receptor alpha (E…
Reviewer #3 (Public Review): CRISPR-Cas9 knockdown of ESR1 in preoptic GABA-kisspeptin neurons abolishes the preovulatory surge and estrous cycles in female mice Open
Evidence suggests that estradiol-sensing preoptic area GABA neurons are involved in the pre-ovulatory surge mechanism necessary for ovulation. In vivo CRISPR-Cas9 editing was used to achieve a 60-70% knockdown in estrogen receptor alpha (E…
CRISPR-Cas9 knockdown of ESR1 in preoptic GABA-kisspeptin neurons suppresses the preovulatory surge and estrous cycles in female mice Open
Evidence suggests that estradiol-sensing preoptic area GABA neurons are involved in the preovulatory surge mechanism necessary for ovulation. In vivo CRISPR-Cas9 editing was used to achieve a 60–70% knockdown in estrogen receptor alpha (ES…
CRISPR-Cas9 knockdown of ESR1 in preoptic GABA-kisspeptin neurons suppresses the preovulatory surge and estrous cycles in female mice Open
Evidence suggests that estradiol-sensing preoptic area GABA neurons are involved in the pre-ovulatory surge mechanism necessary for ovulation. In vivo CRISPR-Cas9 editing was used to achieve a 60-70% knockdown in estrogen receptor alpha (E…
Robust GABAergic Regulation of the GnRH Neuron Distal Dendron Open
The amino acid transmitter γ-aminobutyric acid (GABA) is suspected to play an important role in regulating the activity of the gonadotropin-releasing hormone (GnRH) neurons controlling fertility. Rodent GnRH neurons have a novel dendritic …
Reformulation of PULSAR for Analysis of Pulsatile LH Secretion and a Revised Model of Estrogen-Negative Feedback in Mice Open
The recent use of the tail-tip bleeding approach in mice has enabled researchers to generate detailed pulse and surge profiles of luteinizing hormone (LH) secretion in mice. However, the analysis of pulsatile LH secretion is piecemeal acro…
Highly redundant neuropeptide volume co-transmission underlying episodic activation of the GnRH neuron dendron Open
The necessity and functional significance of neurotransmitter co-transmission remains unclear. The glutamatergic ‘KNDy’ neurons co-express kisspeptin, neurokinin B (NKB), and dynorphin and exhibit a highly stereotyped synchronized behavior…
Indirect Suppression of Pulsatile LH Secretion by CRH Neurons in the Female Mouse Open
Acute stress is a potent suppressor of pulsatile luteinizing hormone (LH) secretion, but the mechanisms through which corticotrophin-releasing hormone (CRH) neurons inhibit gonadotropin-releasing hormone (GnRH) release remain unclear. The …
Innervation of GnRH Neuron Distal Projections and Activation by Kisspeptin in a New GnRH-Cre Rat Model Open
The neural mechanisms generating pulsatile GnRH release from the median eminence (ME) remain unclear. Studies undertaken in the mouse demonstrate that GnRH neurons extend projections to the ME that have properties of both dendrites and axo…
Highly redundant neuropeptide volume co-transmission underlying episodic activation of the GnRH neuron dendron Open
The necessity and functional significance of neurotransmitter co-transmission remains unclear. The glutamatergic “KNDy” neurons co-express kisspeptin, neurokinin B (NKB) and dynorphin and exhibit a highly stereotyped synchronized behavior …
SUN-247 GABA Receptor-Dependent Inhibition and Excitation of GnRH Neuron Dendrons near the Median Eminence in Mice Open
The gonadotrophin-releasing hormone (GnRH) neuron cell bodies are scattered throughout the basal forebrain but funnel their projections to the median eminence to control fertility in all mammals. In mice, these long projections, termed “de…
Genetic Deletion of Esr1 in the Mouse Preoptic Area Disrupts the LH Surge and Estrous Cyclicity Open
Estrogen receptor α (ESR1) is critical for the generation of the preovulatory LH surge. Experiments in rodents have indicated a role for neurons located in the anteroventral periventricular area and preoptic periventricular nucleus [termed…
Dominant Neuropeptide Cotransmission in Kisspeptin-GABA Regulation of GnRH Neuron Firing Driving Ovulation Open
A population of kisspeptin-GABA coexpressing neurons located in the rostral periventricular area of the third ventricle (RP3V) is believed to activate gonadotropin-releasing hormone (GnRH) neurons to generate the luteinizing hormone (LH) s…
View article: Definition of the hypothalamic GnRH pulse generator in mice
Definition of the hypothalamic GnRH pulse generator in mice Open
Significance Neural networks located in the hypothalamus are responsible for generating ultradian patterns of hormone secretion that control a wide variety of functions. How these neural networks generate pulsatile hormone secretion remain…
Dynamics of GnRH Neuron Ionotropic GABA and Glutamate Synaptic Receptors Are Unchanged during Estrogen Positive and Negative Feedback in Female Mice Open
Inputs from GABAergic and glutamatergic neurons are suspected to play an important role in regulating the activity of the gonadotropin-releasing hormone (GnRH) neurons. The GnRH neurons exhibit marked plasticity to control the ovarian cycl…
Spike and Neuropeptide-Dependent Mechanisms Control GnRH Neuron Nerve Terminal Ca <sup>2+</sup> over Diverse Time Scales Open
Fast cell-to-cell communication in the brain is achieved by action potential-dependent synaptic release of neurotransmitters. The fast kinetics of transmitter release are determined by transient Ca 2+ elevations in presynaptic nerve termin…