Robert J. Schaffer
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View article: Analysis on Unveiling the Natural Dynamics of Parthenocarpy and Self-Compatibility in Apple Trees
Analysis on Unveiling the Natural Dynamics of Parthenocarpy and Self-Compatibility in Apple Trees Open
Apple (Malus domestica) is self-incompatible and typically requires cross-pollination for seed and fruit development. Parthenocarpy (fruit development without fertilization) and self-compatibility (fruit set without external pollen) are hi…
View article: The genetic control of herkogamy
The genetic control of herkogamy Open
Herkogamy is the spatial separation of anthers and stigmas within complete flowers, and is a key floral trait that promotes outcrossing in many angiosperms. The degree of separation between pollen-producing anthers and receptive stigmas ha…
View article: Constitutive expression of apple <i>endo‐POLYGALACTURONASE1</i> in fruit induces early maturation, alters skin structure and accelerates softening
Constitutive expression of apple <i>endo‐POLYGALACTURONASE1</i> in fruit induces early maturation, alters skin structure and accelerates softening Open
SUMMARY During fruit ripening, polygalacturonases (PGs) are key contributors to the softening process in many species. Apple is a crisp fruit that normally exhibits only minor changes to cell walls and limited fruit softening. Here, we exp…
View article: A MADS-box gene-induced early flowering pear (Pyrus communis L.) for accelerated pear breeding
A MADS-box gene-induced early flowering pear (Pyrus communis L.) for accelerated pear breeding Open
There have been a considerable number of studies that have successfully sped up the flowering cycle in woody perennial horticultural species. One particularly successful study in apple ( Malus domestica ) accelerated flowering using a silv…
View article: Cell Division Controls Final Fruit Size in Three Apple (Malus x domestica) Cultivars
Cell Division Controls Final Fruit Size in Three Apple (Malus x domestica) Cultivars Open
Apple (Malus x domestica) fruit size is dependent on cell division and cell expansion, processes that are subsequently regulated by plant hormones such as auxins, gibberellins, and cytokinins. In this study, we investigated the role of cel…
View article: Cell division controls final fruit size in three apple (Malus x domestica) cultivars
Cell division controls final fruit size in three apple (Malus x domestica) cultivars Open
Apple (Malus x domestica) fruit size is dependent on cell division and cell expansion, processes which are subsequently regulated by plant hormones such as auxins, gibberellins, and cytokinins. In this study, we investigated the role of ce…
View article: First Chromosome-Scale Assembly and Deep Floral-Bud Transcriptome of a Male Kiwifruit
First Chromosome-Scale Assembly and Deep Floral-Bud Transcriptome of a Male Kiwifruit Open
DATA REPORT article Front. Genet., 16 May 2022Sec. Plant Genomics https://doi.org/10.3389/fgene.2022.852161
View article: Transposon insertions regulate genome‐wide allele‐specific expression and underpin flower colour variations in apple (<i>Malus</i> spp.)
Transposon insertions regulate genome‐wide allele‐specific expression and underpin flower colour variations in apple (<i>Malus</i> spp.) Open
Summary Allele‐specific expression (ASE) can lead to phenotypic diversity and evolution. However, the mechanisms regulating ASE are not well understood, particularly in woody perennial plants. In this study, we investigated ASE genes in th…
View article: Resolving molecular diffusion and aggregation of antibody proteins with megahertz X-ray free-electron laser pulses
Resolving molecular diffusion and aggregation of antibody proteins with megahertz X-ray free-electron laser pulses Open
X-ray free-electron lasers (XFELs) with megahertz repetition rate can provide novel insights into structural dynamics of biological macromolecule solutions. However, very high dose rates can lead to beam-induced dynamics and structural cha…
View article: A Data Driven Approach to Assess Complex Colour Profiles in Plant Tissues
A Data Driven Approach to Assess Complex Colour Profiles in Plant Tissues Open
The ability to quantify the colour of fruit is extremely important for a number of applied fields including plant breeding, postharvest assessment, and consumer quality assessment. Fruit and other plant organs display highly complex colour…
View article: Characterisation of induced Malus x domestica ‘Royal Gala’ cell differentiation by using different hormones in cell cultures
Characterisation of induced Malus x domestica ‘Royal Gala’ cell differentiation by using different hormones in cell cultures Open
Fruit development, following pollination, involves a cell division stage, followed by cell expansion . A major challenges to study each individual stages is the limitations imposed by analyses of fruit development on the plant. Rapid growi…
View article: A Data Driven Approach to Assess Complex Colour Profiles in Plant Tissues
A Data Driven Approach to Assess Complex Colour Profiles in Plant Tissues Open
BackgroundThe ability to quantify the colour of fruit is extremely important for a number of applied fields including plant breeding, postharvest assessment, and consumer quality assessment. Fruit and other plant organs display highly comp…
View article: The Gillenia trifoliata genome reveals dynamics correlated with growth and reproduction in Rosaceae
The Gillenia trifoliata genome reveals dynamics correlated with growth and reproduction in Rosaceae Open
The Rosaceae family has striking phenotypic diversity and high syntenic conservation. Gillenia trifoliata is sister species to the Maleae tribe of apple and ~1000 other species. Gillenia has many putative ancestral features, such as herb/s…
View article: Peridermal fruit skin formation in Actinidia sp. (kiwifruit) is associated with genetic loci controlling russeting and cuticle formation
Peridermal fruit skin formation in Actinidia sp. (kiwifruit) is associated with genetic loci controlling russeting and cuticle formation Open
Background The skin (exocarp) of fleshy fruit is hugely diverse across species. Most fruit types have a live epidermal skin covered by a layer of cuticle made up of cutin while a few create an outermost layer of dead cells (peridermal laye…
View article: Construction of a high-density genetic map for hexaploid kiwifruit (<i>Actinidia chinensis</i> var. <i>deliciosa</i>) using genotyping by sequencing
Construction of a high-density genetic map for hexaploid kiwifruit (<i>Actinidia chinensis</i> var. <i>deliciosa</i>) using genotyping by sequencing Open
Commercially grown kiwifruit (genus Actinidia) are generally of two sub-species which have a base haploid genome of 29 chromosomes. The yellow-fleshed Actinidia chinensis var. chinensis, is either diploid (2n = 2x = 58) or tetraploid (2n =…
View article: Additional file 3 - CKMEXCK_PS1.1.69.0_K857.vcf.gz
Additional file 3 - CKMEXCK_PS1.1.69.0_K857.vcf.gz Open
Compressed Variant call file for SNP changes of K857 population (Actinidia melanandra x Actinidia chinensis backcrossed to Actinidia chinensis) in comparison to published A. chinensis genome (version PS1.1.69.0). Listed as Additional file …
View article: Additional file 3 - CKMEXCK_PS1.1.69.0_K857.vcf.gz
Additional file 3 - CKMEXCK_PS1.1.69.0_K857.vcf.gz Open
Compressed Variant call file for SNP changes of K857 population (Actinidia melanandra x Actinidia chinensis backcrossed to Actinidia chinensis) in comparison to published A. chinensis genome (version PS1.1.69.0). Listed as Additional file …
View article: Kiwifruit maturation, ripening and environmental response is not affected by <i>CENTRORADIALIS</i> ( <i>CEN</i> ) gene-editing
Kiwifruit maturation, ripening and environmental response is not affected by <i>CENTRORADIALIS</i> ( <i>CEN</i> ) gene-editing Open
Previous studies have shown the conversion of the perennial kiwifruit (Actinidia spp.) to continuously flowering allows rapid cycling of generations. These plants are smaller, flower earlier and do not undergo dormancy. They provide a mode…
View article: Additional file 4 of A gene expression atlas for kiwifruit (Actinidia chinensis) and network analysis of transcription factors
Additional file 4 of A gene expression atlas for kiwifruit (Actinidia chinensis) and network analysis of transcription factors Open
Additional file 4: Additional data 4. Table of TPM for the RNA-seq data.
View article: Coreless apples generated by the suppression of carpel genes and hormone-induced fruit set
Coreless apples generated by the suppression of carpel genes and hormone-induced fruit set Open
Seedless fruits have high consumer appeal and have made seeded varieties obsolete in some crops. In seedless apple varieties, core tissues which normally contain the seed can be unpalatable, reducing the seedless appeal. Apples are accesso…
View article: Additional file 3 of A gene expression atlas for kiwifruit (Actinidia chinensis) and network analysis of transcription factors
Additional file 3 of A gene expression atlas for kiwifruit (Actinidia chinensis) and network analysis of transcription factors Open
Additional file 3: Additional data 3. Details of the RNA-seq data used in this study.
View article: Additional file 1 of A gene expression atlas for kiwifruit (Actinidia chinensis) and network analysis of transcription factors
Additional file 1 of A gene expression atlas for kiwifruit (Actinidia chinensis) and network analysis of transcription factors Open
Additional file 1: Additional data 1. Table of transcription factors, with corresponding colour module assignments and connectivity.
View article: Additional file 6 of A gene expression atlas for kiwifruit (Actinidia chinensis) and network analysis of transcription factors
Additional file 6 of A gene expression atlas for kiwifruit (Actinidia chinensis) and network analysis of transcription factors Open
Additional file 6: Additional data 6. Numbers of transcription factor classes within each colour module.
View article: An Actinidia chinensis (kiwifruit) eFP browser and network analysis of transcription factors
An Actinidia chinensis (kiwifruit) eFP browser and network analysis of transcription factors Open
BackgroundTranscriptomic studies combined with a well annotated genome have laid the foundations for new understanding of molecular processes. Tools which visualise gene expression patterns have further added to these resources. The manual…
View article: Fruit From Two Kiwifruit Genotypes With Contrasting Softening Rates Show Differences in the Xyloglucan and Pectin Domains of the Cell Wall
Fruit From Two Kiwifruit Genotypes With Contrasting Softening Rates Show Differences in the Xyloglucan and Pectin Domains of the Cell Wall Open
Fruit softening is controlled by hormonal and developmental cues, causing an upregulation of cell wall-associated enzymes that break down the complex sugar matrices in the cell wall. The regulation of this process is complex, with differen…
View article: A review of current knowledge about the formation of native peridermal exocarp in fruit
A review of current knowledge about the formation of native peridermal exocarp in fruit Open
The outer skin layer in any plant is essential in offering a protective barrier against water loss and pathogen attack. Within fleshy fruit, the skin supports internal cell layers and can provide the initial cues in attracting seed-dispers…