Roby P. Bhattacharyya
YOU?
Author Swipe
View article: The carbapenem inoculum effect provides insights into the molecular mechanisms underlying carbapenem resistance in the Enterobacterales
The carbapenem inoculum effect provides insights into the molecular mechanisms underlying carbapenem resistance in the Enterobacterales Open
Carbapenem-resistant Enterobacterales (CRE) are important pathogens that can develop resistance via multiple molecular mechanisms, including hydrolysis or reduced antibiotic influx. Identifying these mechanisms can improve pathogen surveil…
View article: A multimodal atlas of COVID-19 severity identifies hallmarks of dysregulated immunity
A multimodal atlas of COVID-19 severity identifies hallmarks of dysregulated immunity Open
The alpha-variant wave of the COVID-19 pandemic provided a unique opportunity to study, at single-cell resolution, how near-universal exposure to the same pathogen can lead to either effective or dysfunctional immune responses in humans. A…
View article: BADLOCK: A Rapid, Portable, Inexpensive Diagnostic for Bacterial Pathogen and Resistance Detection in Resource-Limited Settings
BADLOCK: A Rapid, Portable, Inexpensive Diagnostic for Bacterial Pathogen and Resistance Detection in Resource-Limited Settings Open
Antimicrobial resistance is a major global health threat, with disproportionate impact in regions with limited diagnostic infrastructure. To address this challenge, we developed BADLOCK (Bacterial and AMR Detection by SHERLOCK), a rapid, l…
View article: Longitudinal Immune Profiling in Sepsis Reveals Transient Expansion of a CD14<sup>+</sup>Monocyte State and Persistent T Cell Suppression
Longitudinal Immune Profiling in Sepsis Reveals Transient Expansion of a CD14<sup>+</sup>Monocyte State and Persistent T Cell Suppression Open
Sepsis is a dynamic syndrome of immune dysregulation and a leading cause of global mortality. Although immune suppression is recognized as a hallmark of sepsis, the temporal dynamics and mechanistic drivers of immune dysfunction remain inc…
View article: Speeding up drug susceptibility testing in Mycobacterium tuberculosis using RNA biomarkers
Speeding up drug susceptibility testing in Mycobacterium tuberculosis using RNA biomarkers Open
Institut Pasteur, Agence Nationale de la Recherche.
View article: P-2245. Single cell RNA-sequencing of immune cells demonstrates differential expansion of a monocyte transcriptional state among the Sepsis Response Signature (SRS) endotypes
P-2245. Single cell RNA-sequencing of immune cells demonstrates differential expansion of a monocyte transcriptional state among the Sepsis Response Signature (SRS) endotypes Open
Background Immune response variation among sepsis patients leads to substantial clinical heterogeneity that makes treatment challenging. Bulk RNA-sequencing of circulating immune cells has been used in an attempt to resolve sepsis heteroge…
View article: P-2112. Development and Validation of a Cheap, Portable Assay to Rapidly Profile Bacterial Infections of the Bloodstream
P-2112. Development and Validation of a Cheap, Portable Assay to Rapidly Profile Bacterial Infections of the Bloodstream Open
Background Antimicrobial resistant (AMR) bacterial infections pose a significant and growing health threat worldwide, and their burden is most severe in lower income areas where diagnostic infrastructure is lacking. The development of cost…
View article: P-2219. Demonstrating a Novel Immune Cell Isolation Strategy for Scalable Single-Cell RNA Sequencing Studies in Patients with Sepsis
P-2219. Demonstrating a Novel Immune Cell Isolation Strategy for Scalable Single-Cell RNA Sequencing Studies in Patients with Sepsis Open
Background Though immune dysregulation often plays a key role in disease, characterizing circulating immune cells in critical illnesses is challenging. Studies using single-cell RNA sequencing (scRNA-seq) of peripheral blood mononuclear ce…
View article: The Effect of Nirmatrelvir-Ritonavir on Short- and Long-term Adverse Outcomes From COVID-19 Among Patients With Kidney Disease: A Propensity Score–Matched Study
The Effect of Nirmatrelvir-Ritonavir on Short- and Long-term Adverse Outcomes From COVID-19 Among Patients With Kidney Disease: A Propensity Score–Matched Study Open
Background Patients with kidney disease are at high risk for adverse outcomes after coronavirus disease 2019 (COVID-19) despite vaccination. Because patients with advanced chronic kidney disease (CKD) and kidney failure were excluded from …
View article: Clinical pilot of bacterial transcriptional profiling as a combined genotypic and phenotypic antimicrobial susceptibility test
Clinical pilot of bacterial transcriptional profiling as a combined genotypic and phenotypic antimicrobial susceptibility test Open
Antimicrobial resistance is a growing health threat, but standard methods for determining antibiotic susceptibility are slow and can delay optimal treatment, which is especially consequential in severe infections such as bacteremia. Novel …
View article: Cryo-PRO facilitates whole blood cryopreservation for single-cell RNA sequencing of immune cells from clinical samples
Cryo-PRO facilitates whole blood cryopreservation for single-cell RNA sequencing of immune cells from clinical samples Open
Single-cell RNA sequencing (scRNA-seq) of peripheral blood mononuclear cells (PBMCs) has enhanced our understanding of host immune mechanisms in small cohorts, particularly in diseases with complex and heterogeneous immune responses such a…
View article: Clinical Pilot of Bacterial Transcriptional Profiling as a Combined Genotypic and Phenotypic Antimicrobial Susceptibility Test
Clinical Pilot of Bacterial Transcriptional Profiling as a Combined Genotypic and Phenotypic Antimicrobial Susceptibility Test Open
Antimicrobial resistance is a growing health threat, but standard methods for determining antibiotic susceptibility are slow and can delay optimal treatment, which is especially consequential in severe infections such as bacteremia. Novel …
View article: Patients with end-stage kidney disease and COVID-19 are commonly hospitalized early during COVID-19 illness: an opportunity for early intervention
Patients with end-stage kidney disease and COVID-19 are commonly hospitalized early during COVID-19 illness: an opportunity for early intervention Open
Antiviral medications such as remdesivir, molnupiravir, and nirmatrelvir/ritonavir are most effective when used early in the course of coronavirus disease 2019 (COVID-19). These medications are mainly authorized for outpatient use in high …
View article: Candida albicans extracellular vesicles trigger type I IFN signalling via cGAS and STING
Candida albicans extracellular vesicles trigger type I IFN signalling via cGAS and STING Open
View article: 875. Development of a Cheap, Portable Assay to Rapidly Profile Resistant Bacterial Infections
875. Development of a Cheap, Portable Assay to Rapidly Profile Resistant Bacterial Infections Open
Background Antimicrobial resistant (AMR) bacterial infections pose a significant and growing health threat worldwide. The burden of these resistant infections is often most severe in lower income areas, where the medical infrastructure nec…
View article: 250. Single cell profiling of blood immune cell kinetics in early sepsis reveals progressive decrease in a monocyte substate with immunosuppressive activity
250. Single cell profiling of blood immune cell kinetics in early sepsis reveals progressive decrease in a monocyte substate with immunosuppressive activity Open
Background Dysregulation of the immune response to bacterial infection is an essential but poorly understood component of sepsis. Kinetic changes in immune cell quantity during sepsis can reveal key elements of immunopathogenesis, yet thes…
View article: 623. Advancing Fungal Diagnostics with a Transcriptional Approach
623. Advancing Fungal Diagnostics with a Transcriptional Approach Open
Background Invasive fungal infections are increasingly common and carry high rates of morbidity and mortality, and rising rates of drug resistance, notably in Candida auris. However, there is a notable gap between the development of bacter…
View article: Clinical and Genomic Characterization of a Cohort of Patients With <i>Klebsiella pneumoniae</i> Bloodstream Infection
Clinical and Genomic Characterization of a Cohort of Patients With <i>Klebsiella pneumoniae</i> Bloodstream Infection Open
Background The clinical and microbial factors associated with Klebsiella pneumoniae bloodstream infections (BSIs) are not well characterized. Prior studies have focused on highly resistant or hypervirulent isolates, limiting our understand…
View article: The carbapenem inoculum effect provides insight into the molecular mechanisms underlying carbapenem resistance in Enterobacterales
The carbapenem inoculum effect provides insight into the molecular mechanisms underlying carbapenem resistance in Enterobacterales Open
Carbapenem-resistant Enterobacterales (CRE) are important pathogens that can develop resistance via multiple molecular mechanisms, including hydrolysis or reduced antibiotic influx. Identifying these mechanisms can improve pathogen surveil…
View article: Bacterial droplet-based single-cell RNA-seq reveals antibiotic-associated heterogeneous cellular states
Bacterial droplet-based single-cell RNA-seq reveals antibiotic-associated heterogeneous cellular states Open
View article: Cloacaenodin, an Antimicrobial Lasso Peptide with Activity against <i>Enterobacter</i>
Cloacaenodin, an Antimicrobial Lasso Peptide with Activity against <i>Enterobacter</i> Open
Using genome mining and heterologous expression, we report the discovery and production of a new antimicrobial lasso peptide from species related to the Enterobacter cloacae complex. Using NMR and mass spectrometric analysis, we sho…
View article: Pre-Existing Population Immunity and severe acute respiratory syndrome coronavirus 2 Variant Establishment and Dominance Dynamics in the United States: An Ecological Study
Pre-Existing Population Immunity and severe acute respiratory syndrome coronavirus 2 Variant Establishment and Dominance Dynamics in the United States: An Ecological Study Open
We conducted an ecological analysis of the dynamics of Delta and Omicron establishment and dominance in US states. Omicron became the dominant circulating variant later in states with higher population-level immunity. By contrast, populati…
View article: 145. Harnessing the Inoculum Effect to Diagnose Molecular Mechanisms of Carbapenem Resistance
145. Harnessing the Inoculum Effect to Diagnose Molecular Mechanisms of Carbapenem Resistance Open
Background The global spread of carbapenem-resistant Enterobacterales (CRE) presents a major threat to public health. CRE employ two molecular mechanisms to evade carbapenems: 1) expression carbapenemases (CPases), which efficiently hydrol…
View article: 1964. Pre-existing Population Immunity and SARS-CoV-2 Variant Dynamics in the United States: An Ecological Study
1964. Pre-existing Population Immunity and SARS-CoV-2 Variant Dynamics in the United States: An Ecological Study Open
Background The relative advantage of each new variant of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) depends on its inherent transmissibility and capacity to evade pre-existing immunity. Delta and Omicron are variants of c…
View article: Longitudinal characterization of circulating neutrophils uncovers phenotypes associated with severity in hospitalized COVID-19 patients
Longitudinal characterization of circulating neutrophils uncovers phenotypes associated with severity in hospitalized COVID-19 patients Open
View article: A novel rRNA hybridization-based approach to rapid, accurate <i>Candida</i> identification directly from blood culture
A novel rRNA hybridization-based approach to rapid, accurate <i>Candida</i> identification directly from blood culture Open
Invasive fungal infections are increasingly common and carry high morbidity and mortality, yet fungal diagnostics lag behind bacterial diagnostics in rapidly identifying the causal pathogen. We previously devised a fluorescent hybridizatio…
View article: Bacterial droplet-based single-cell RNA-seq reveals heterogeneity in bacterial populations and in response to antibiotic perturbation
Bacterial droplet-based single-cell RNA-seq reveals heterogeneity in bacterial populations and in response to antibiotic perturbation Open
We introduce BacDrop, a highly scalable technology for bacterial single-cell RNA sequencing that has overcome many challenges hindering the development of scRNA-seq in bacteria. BacDrop can be applied to thousands or millions of cells from…
View article: Pre-existing population immunity and SARS-CoV-2 variant establishment and dominance dynamics in the United States: An ecological study
Pre-existing population immunity and SARS-CoV-2 variant establishment and dominance dynamics in the United States: An ecological study Open
We conducted an ecological analysis of the dynamics of Delta and Omicron establishment and dominance in U.S. states. Omicron became the dominant circulating variant later in states with higher population-level immunity. By contrast, popula…
View article: Gilteritinib or Chemotherapy for Relapsed or Refractory <i>FLT3</i> -Mutated AML
Gilteritinib or Chemotherapy for Relapsed or Refractory <i>FLT3</i> -Mutated AML Open
View article: A novel rRNA hybridization-based approach to rapid, accurate <i>Candida</i> identification directly from blood culture
A novel rRNA hybridization-based approach to rapid, accurate <i>Candida</i> identification directly from blood culture Open
Invasive fungal infections are increasingly common and carry high morbidity and mortality, yet fungal diagnostics lag behind bacterial diagnostics in rapidly identifying the causal pathogen. We previously devised a fluorescent hybridizatio…