Robyn Roth
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View article: Guanine storage vacuoles sequester guanine crystals and serve as nitrogen reserves in <i>Chlamydomonas reinhardtii</i>
Guanine storage vacuoles sequester guanine crystals and serve as nitrogen reserves in <i>Chlamydomonas reinhardtii</i> Open
Nitrogen cycles in Chlamydomonas reinhardtii have historically been studied in the context of exogenous ammonium/nitrate availability and uptake. Recently, crystalline guanine has been identified, using Raman microscopy, in the cytoplasm o…
View article: Ultrastructure of the Endoplasmic Reticulum in Eukaryotic Microalgae
Ultrastructure of the Endoplasmic Reticulum in Eukaryotic Microalgae Open
The endoplasmic reticulum (ER) is a large and highly dynamic component of the eukaryotic endomembrane system. In eukaryotic microalgae, it plays six distinct roles: (1) It envelopes the chromatin to form the nucleus . (2) It forms cisterna…
View article: Distinct Patterns of Smooth Muscle Phenotypic Modulation in Thoracic and Abdominal Aortic Aneurysms
Distinct Patterns of Smooth Muscle Phenotypic Modulation in Thoracic and Abdominal Aortic Aneurysms Open
Thoracic and abdominal aortic aneurysms (TAAs and AAAs, respectively) share morphological features but have distinct clinical and hereditary characteristics. Studies using bulk RNA comparisons revealed distinct patterns of gene expression …
View article: Poglut2/3 double knockout in mice results in neonatal lethality with reduced levels of fibrillin in lung tissues
Poglut2/3 double knockout in mice results in neonatal lethality with reduced levels of fibrillin in lung tissues Open
Fibrillin microfibrils play a critical role in the formation of elastic fibers, tissue/organ development, and cardiopulmonary function. These microfibrils not only provide structural support and flexibility to tissues, but they also regula…
View article: Immunogenic profile of a plant-produced nonavalent African horse sickness viral protein 2 (VP2) vaccine in IFNAR-/- mice
Immunogenic profile of a plant-produced nonavalent African horse sickness viral protein 2 (VP2) vaccine in IFNAR-/- mice Open
A safe, highly immunogenic multivalent vaccine to protect against all nine serotypes of African horse sickness virus (AHSV), will revolutionise the AHS vaccine industry in endemic countries and beyond. Plant-produced AHS virus-like particl…
View article: Multi‐organ phenotypes in mice lacking latent <scp>TGFβ</scp> binding protein 2 (<scp>LTBP2</scp>)
Multi‐organ phenotypes in mice lacking latent <span>TGFβ</span> binding protein 2 (<span>LTBP2</span>) Open
Background Latent TGFβ binding protein‐2 (LTBP2) is a fibrillin 1 binding component of the microfibril. LTBP2 is the only LTBP protein that does not bind any isoforms of TGFβ, although it may interfere with the function of other LTBPs or i…
View article: Immunogenicity of adjuvanted plant-produced SARS-CoV-2 Beta spike VLP vaccine in New Zealand white rabbits
Immunogenicity of adjuvanted plant-produced SARS-CoV-2 Beta spike VLP vaccine in New Zealand white rabbits Open
The outbreak of the SARS-CoV-2 global pandemic heightened the pace of vaccine development with various vaccines being approved for human use in a span of 24 months. The SARS-CoV-2 trimeric spike (S) surface glycoprotein, which mediates vir…
View article: A new mouse model of elastin haploinsufficiency highlights the importance of elastin to vascular development and blood pressure regulation
A new mouse model of elastin haploinsufficiency highlights the importance of elastin to vascular development and blood pressure regulation Open
Supravalvular aortic stenosis (SVAS) is an autosomal dominant disease resulting from elastin (ELN) haploinsufficiency. Individuals with SVAS typically develop a thickened arterial media with an increased number of elastic lamellae and smoo…
View article: Membrane repair triggered by cholesterol-dependent cytolysins is activated by mixed lineage kinases and MEK
Membrane repair triggered by cholesterol-dependent cytolysins is activated by mixed lineage kinases and MEK Open
Repair of plasma membranes damaged by bacterial pore-forming toxins, such as streptolysin O or perfringolysin O, during septic cardiomyopathy or necrotizing soft tissue infections is mediated by several protein families. However, the activ…
View article: Loss of Angiotensin II Type 2 Receptor Improves Blood Pressure in Elastin Insufficiency
Loss of Angiotensin II Type 2 Receptor Improves Blood Pressure in Elastin Insufficiency Open
There is ample evidence supporting a role for angiotensin II type 2 receptor (AT 2 R) in counterbalancing the effects of angiotensin II (ang II) through the angiotensin II type 1 receptor by promoting vasodilation and having anti-inflammat…
View article: Lichen 2. Constituents
Lichen 2. Constituents Open
Lichens are slow-growing niche-constructing organisms that form a thallus via scripted symbiotic/mutualist relationships between fungi, algae, and bacteria. Here we use quick-freeze deep-etch electron microscopy (QFDEEM) to document the ul…
View article: Lichen 4. The Algal Layer
Lichen 4. The Algal Layer Open
A lichen is a slow-growing niche-constructing organism that forms a thallus via scripted symbiotic/mutualist relationships between fungi, algae, and bacteria. Here we use quick-freeze deep-etch electron microscopy (QFDEEM) and light micros…
View article: Lichen 1. Solo fungal and algal partners
Lichen 1. Solo fungal and algal partners Open
Lichens are slow-growing niche-constructing organisms that form a thallus via scripted symbiotic/mutualistic relationships between fungi, algae, and bacteria, and that are distributed across nearly all terrestrial ecosystems. Here we use q…
View article: Lichen 5. Medullary and bacterial biofilm layers
Lichen 5. Medullary and bacterial biofilm layers Open
A lichen is a slow-growing niche-constructing organism that form a thallus via scripted symbiotic/mutualist relationships between fungi, algae, and bacteria. In preceding papers we have presented quick-freeze deep-etch EM (QFDEEM) images o…
View article: 3D Visualization of the Podocyte Actin Network using Integrated Membrane Extraction, Electron Microscopy, and Deep Learning
3D Visualization of the Podocyte Actin Network using Integrated Membrane Extraction, Electron Microscopy, and Deep Learning Open
Although actin stress fibers are abundant in cultured cells, little is known about these structures in vivo. In podocytes of the kidney glomerulus, much evidence suggests that mechanobiological mechanisms underlie injury, with changes to a…
View article: TAT-RasGAP<sub>317-326</sub>kills cells by targeting inner-leaflet–enriched phospholipids
TAT-RasGAP<sub>317-326</sub>kills cells by targeting inner-leaflet–enriched phospholipids Open
Significance TAT-RasGAP 317–326 can lyse cancer cells in a manner distinct from known programmed cell death pathways through its ability to target specific plasma membrane lipids. The killing properties of this peptide may therefore be dif…
View article: The AP-2 Adaptor 2 Appendage Scaffolds Alternate Cargo Endocytosis
The AP-2 Adaptor 2 Appendage Scaffolds Alternate Cargo Endocytosis Open
The independently folded appendages of the large α and β2 subunits of the endocytic adaptor protein (AP)-2 complex coordinate proper assembly and operation of endocytic components during clathrin-mediated endocytosis. The β2 subunit append…
View article: Encephalitic Alphaviruses Exploit Caveola-Mediated Transcytosis at the Blood-Brain Barrier for Central Nervous System Entry
Encephalitic Alphaviruses Exploit Caveola-Mediated Transcytosis at the Blood-Brain Barrier for Central Nervous System Entry Open
VEEV, WEEV, and eastern equine encephalitis virus (EEEV) are emerging infectious diseases in the Americas, and they have caused several major outbreaks in the human and horse population during the past few decades. Shortly after infection,…
View article: Contacting domains that segregate lipid from solute transporters in malaria parasites
Contacting domains that segregate lipid from solute transporters in malaria parasites Open
While membrane contact sites (MCS) between intracellular organelles are abundant 1 , and cell-cell junctions are classically defined 2 , very little is known about the contacts between membranes that delimit extracellular junctions within …
View article: Encephalitic alphaviruses exploit caveolae-mediated transcytosis at the blood-brain barrier for CNS entry
Encephalitic alphaviruses exploit caveolae-mediated transcytosis at the blood-brain barrier for CNS entry Open
Venezuelan and Western equine encephalitis viruses (VEEV and WEEV) invade the CNS early during infection, via neuronal and hematogenous routes (1, 2). While viral replication mediates host-shut off, including expression of type I interfero…
View article: Heterogeneous Cellular Contributions to Elastic Laminae Formation in Arterial Wall Development
Heterogeneous Cellular Contributions to Elastic Laminae Formation in Arterial Wall Development Open
Rationale: Elastin is an important ECM (extracellular matrix) protein in large and small arteries. Vascular smooth muscle cells (SMCs) produce the layered elastic laminae found in elastic arteries but synthesize little elastin in muscular …
View article: A Rubisco-binding protein is required for normal pyrenoid number and starch sheath morphology in <i>Chlamydomonas reinhardtii</i>
A Rubisco-binding protein is required for normal pyrenoid number and starch sheath morphology in <i>Chlamydomonas reinhardtii</i> Open
Significance Many cellular structures are assembled via phase separation, forming liquid-like droplets in a manner analogous to oil in water. How can the cell control whether there is 1 droplet or many? Here, we provide insights into this …
View article: Analysis of an improved Cyanophora paradoxa genome assembly
Analysis of an improved Cyanophora paradoxa genome assembly Open
Glaucophyta are members of the Archaeplastida, the founding group of photosynthetic eukaryotes that also includes red algae (Rhodophyta), green algae, and plants (Viridiplantae). Here we present a high-quality assembly, built using long-re…