Roger Benoit
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View article: Regulation of p65 nuclear localization and chromatin states by compressive force
Regulation of p65 nuclear localization and chromatin states by compressive force Open
The tumor microenvironment (TME) is a dynamic ecosystem, that evolves with the developing tumor to support its growth and metastasis. A key aspect of TME evolution is the recruitment of stromal fibroblasts, carried out via the release of v…
View article: Development of radiofluorinated MLN-4760 derivatives for PET imaging of the SARS-CoV-2 entry receptor ACE2
Development of radiofluorinated MLN-4760 derivatives for PET imaging of the SARS-CoV-2 entry receptor ACE2 Open
Purpose The angiotensin converting enzyme 2 (ACE2) plays a regulatory role in the cardiovascular system and serves SARS-CoV-2 as an entry receptor. The aim of this study was to synthesize and evaluate radiofluorinated derivatives of the AC…
View article: Semisynthetic LC3 Probes for Autophagy Pathways Reveal a Noncanonical LC3 Interacting Region Motif Crucial for the Enzymatic Activity of Human ATG3
Semisynthetic LC3 Probes for Autophagy Pathways Reveal a Noncanonical LC3 Interacting Region Motif Crucial for the Enzymatic Activity of Human ATG3 Open
Macroautophagy is one of two major degradation systems in eukaryotic cells. Regulation and control of autophagy are often achieved through the presence of short peptide sequences called LC3 interacting regions (LIR) in autophagy-involved p…
View article: OligoBinders: Bioengineered Soluble Amyloid-like Nanoparticles to Bind and Neutralize SARS-CoV-2
OligoBinders: Bioengineered Soluble Amyloid-like Nanoparticles to Bind and Neutralize SARS-CoV-2 Open
[Image: see text] The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection has become a primary health concern. Molecules that prevent viral entry into host cells by …
View article: Human ATG3 contains a non-canonical LIR motif crucial for its enzymatic activity in autophagy
Human ATG3 contains a non-canonical LIR motif crucial for its enzymatic activity in autophagy Open
Macroautophagy is one of two major degradation systems in eukaryotic cells. Regulation and control of autophagy is often achieved through the presence of short peptide sequences called LC3 interacting regions (LIR) in autophagy-involved pr…
View article: Cryo-EM structure of a single-chain β1-adrenoceptor – AmpC β-lactamase fusion protein
Cryo-EM structure of a single-chain β1-adrenoceptor – AmpC β-lactamase fusion protein Open
The insertion of fusion proteins has enabled the crystallization of a wide range of G-protein-coupled receptors. Here, we adapted this engineering strategy to cryo-electron microscopy (cryo-EM). We inserted the soluble protein AmpC β-lacta…
View article: Design, Expression, Purification, and Characterization of a YFP-Tagged 2019-nCoV Spike Receptor-Binding Domain Construct
Design, Expression, Purification, and Characterization of a YFP-Tagged 2019-nCoV Spike Receptor-Binding Domain Construct Open
2019-nCoV is the causative agent of the serious, still ongoing, worldwide coronavirus disease (COVID-19) pandemic. High quality recombinant virus proteins are required for research related to the development of vaccines and improved assays…
View article: Chimeric single α-helical domains as rigid fusion protein connections for protein nanotechnology and structural biology
Chimeric single α-helical domains as rigid fusion protein connections for protein nanotechnology and structural biology Open
Summary Chimeric fusion proteins are essential tools for protein nanotechnology. Non-optimized protein-protein connections are usually flexible, which makes them unsuitable as structural building blocks. Here we show that the ER/K motif, a…
View article: Design, expression, purification and characterization of a YFP-tagged 2019-nCoV spike receptor-binding domain construct
Design, expression, purification and characterization of a YFP-tagged 2019-nCoV spike receptor-binding domain construct Open
2019-nCoV is the causative agent of the serious, still ongoing, worldwide COVID-19 pandemic. High quality recombinant virus proteins are required for research related to the development of vaccines and improved assays, and to the general u…
View article: Botulinum Neurotoxin Diversity from a Gene-Centered View
Botulinum Neurotoxin Diversity from a Gene-Centered View Open
Botulinum neurotoxins (BoNTs) rank amongst the most potent toxins known. The factors responsible for the emergence of the many known and yet unknown BoNT variants remain elusive. It also remains unclear why anaerobic bacteria that are wide…
View article: Crystal structure of the BoNT/A2 receptor-binding domain in complex with the luminal domain of its neuronal receptor SV2C
Crystal structure of the BoNT/A2 receptor-binding domain in complex with the luminal domain of its neuronal receptor SV2C Open
A detailed molecular understanding of botulinum neurotoxin (BoNT)/host-cell-receptor interactions is fundamental both for developing strategies against botulism and for generating improved BoNT variants for medical applications. The X-ray …
View article: Structural basis for misregulation of kinesin KIF21A autoinhibition by CFEOM1 disease mutations
Structural basis for misregulation of kinesin KIF21A autoinhibition by CFEOM1 disease mutations Open
Tight regulation of kinesin activity is crucial and malfunction is linked to neurological diseases. Point mutations in the KIF21A gene cause congenital fibrosis of the extraocular muscles type 1 (CFEOM1) by disrupting the autoinhibitory in…
View article: Seamless Insert-Plasmid Assembly at High Efficiency and Low Cost
Seamless Insert-Plasmid Assembly at High Efficiency and Low Cost Open
Seamless cloning methods, such as co-transformation cloning, sequence- and ligation-independent cloning (SLIC) or the Gibson assembly, are essential tools for the precise construction of plasmids. The efficiency of co-transformation clonin…