Ron Kelley
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View article: Towards community-driven visual proteomics with large-scale cryo-electron tomography of <i>Chlamydomonas reinhardtii</i>
Towards community-driven visual proteomics with large-scale cryo-electron tomography of <i>Chlamydomonas reinhardtii</i> Open
In situ cryo-electron tomography (cryo-ET) has emerged as the method of choice to investigate structures of biomolecules in their native context. However, challenges remain in the efficient production of large-scale cryo-ET datasets, as we…
View article: In-cell architecture of the mitochondrial respiratory chain
In-cell architecture of the mitochondrial respiratory chain Open
Mitochondria produce energy through oxidative phosphorylation, carried out by five membrane-bound complexes collectively known as the respiratory chain. These complexes work in concert to transfer electrons and pump protons, leading to ATP…
View article: Abstract 1475 High-throughput Collaborative in situ Cryo-ET Enables the Molecular Atlas of C. reinhardtii
Abstract 1475 High-throughput Collaborative in situ Cryo-ET Enables the Molecular Atlas of C. reinhardtii Open
In situ Cryo-Electron Tomography (Cryo-ET) has emerged as the method of choice to investigate the structures of biomolecules in their native context. Currently, thicker unicellular and, more recently, multicellular samples are machined usi…
View article: Deep reconstructing generative networks for visualizing dynamic biomolecules inside cells
Deep reconstructing generative networks for visualizing dynamic biomolecules inside cells Open
Advances in cryo-electron tomography (cryo-ET) have produced new opportunities to visualize the structures of dynamic macromolecular machinery in native cellular environments. Here, we describe a machine learning approach that can reconstr…
View article: Hydra Plasma FIB DualBeam for High-resolution Cryo Auto Slice & View and Reliable Lamella Preparation for Cellular and Tissue Samples
Hydra Plasma FIB DualBeam for High-resolution Cryo Auto Slice & View and Reliable Lamella Preparation for Cellular and Tissue Samples Open
Journal Article Hydra Plasma FIB DualBeam for High-resolution Cryo Auto Slice & View and Reliable Lamella Preparation for Cellular and Tissue Samples Get access Ron Kelley, Ron Kelley Thermo Fisher Scientific, Hillsboro, OR, USA Search for…
View article: Towards the Visual Proteomics of <i>C. reinhardtii</i> using High-throughput Collaborative <i>in situ</i> Cryo-ET
Towards the Visual Proteomics of <i>C. reinhardtii</i> using High-throughput Collaborative <i>in situ</i> Cryo-ET Open
Journal Article Towards the Visual Proteomics of C. reinhardtii using High-throughput Collaborative in situ Cryo-ET Get access Sagar Khavnekar, Sagar Khavnekar Cryo-EM Technology group, Max Planck Institute of Biochemistry, Martinsried, Ge…
View article: Cryo-plasma FIB/SEM volume imaging of biological specimens
Cryo-plasma FIB/SEM volume imaging of biological specimens Open
Serial focussed ion beam scanning electron microscopy (FIB/SEM) enables imaging and assessment of subcellular structures on the mesoscale (10 nm to 10 µm). When applied to vitrified samples, serial FIB/SEM is also a means to target specifi…
View article: Cryo-plasma FIB/SEM volume imaging of biological specimens
Cryo-plasma FIB/SEM volume imaging of biological specimens Open
Serial focussed ion beam scanning electron microscopy (FIB/SEM) enables imaging and assessment of sub-cellular structures on the mesoscale (10 nm to 10 μm). When applied to vitrified samples, serial FIB/SEM is also a means to target specif…
View article: Optimizing Cryo-FIB Lamellas for sub-5Å in situ Structural Biology
Optimizing Cryo-FIB Lamellas for sub-5Å in situ Structural Biology Open
We here present a method based on metallic platinum sputtering that can substantially enhance the quality of subtomogram averages from lamellas and thereby reduce the number of particles needed for high-resolution subtomogram averaging. We…
View article: Incorporation of Ti in epitaxial Fe <sub>2</sub> TiO <sub>4</sub> thin films
Incorporation of Ti in epitaxial Fe <sub>2</sub> TiO <sub>4</sub> thin films Open
The titanomagnetites (Fe 2− x Ti x O 4 , x ⩽ 1) are a family of reducible spinel-structure oxides of interest for their favorable magnetic, catalytic, and electrical transport properties. To understand the stability of the system during lo…
View article: Correlative Imaging of Phase Separation in Fe2TiO4 Thin Films Prepared by Conventional Ga and Xe Plasma FIB Processing
Correlative Imaging of Phase Separation in Fe2TiO4 Thin Films Prepared by Conventional Ga and Xe Plasma FIB Processing Open
Journal Article Correlative Imaging of Phase Separation in Fe2TiO4 Thin Films Prepared by Conventional Ga and Xe Plasma FIB Processing Get access Steven Spurgeon, Steven Spurgeon Pacific Northwest National Laboratory, Richland, Washington,…
View article: Investigation of slice thickness for FIB tomography in a plasma focused ion beam system
Investigation of slice thickness for FIB tomography in a plasma focused ion beam system Open
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