Explanipedia

Generation and characterization of a mouse model of conditional <i>Chd4</i> knockout in the endometrial epithelium Open

Shannon Harkins, Hilary Skalski, Abigail Z. Bennett, Laura A. Pavliscak, Amelia R Arendt , et al. · 2025

Chromatin remodeling plays an integral part in endometrial homeostasis, having roles in the maintenance of cell identity, epithelial integrity, and prevention of endometrial disease. Chromodomain-helicase-DNA-binding protein 4 ( CHD4 ) is …

WERF Endometriosis Phenome and Biobanking Harmonisation Project for Experimental Models in Endometriosis Research (EPHect-EM-Organoids): endometrial organoids as an emerging technology for endometriosis research Open

Elizabeth E. Marr, Juan S. Gnecco, Stacey A. Missmer, Shannon M. Hawkins, Kevin G. Osteen , et al. · 2025

The aetiology of endometriosis remains poorly understood. In vitro model systems provide the opportunity to identify the mechanisms driving disease pathogenesis using human cells. Three-dimensional models, particularly organoid systems, ha…

WERF Endometriosis Phenome and Biobanking Harmonisation Project for Experimental Models in Endometriosis Research (EPHect-EM-Heterologous): heterologous rodent models Open

M. Louise Hull, Raul Gomez, Warren B. Nothnick, Ruth Grümmer, Katherine A. Burns , et al. · 2025

Endometriosis, defined as the growth of endometrial-like tissues outside the uterus, is a common disease among women. Numerous in vivo rodent models of endometriosis have been developed to explore multiple aspects of this poorly understood…

WERF Endometriosis Phenome and Biobanking Harmonisation Project for Experimental Models in Endometriosis Research (EPHect-EM-Homologous): homologous rodent models Open

Katherine A. Burns, Daniëlle Peterse, Caroline B. Appleyard, Ronald L. Chandler, Sun‐Wei Guo , et al. · 2025

In vivo models of endometriosis enable the discovery and preclinical testing of new therapies. Several rodent models of endometriosis exist, but a lack of harmonization impedes reproducibility and comparability of results among investigato…

WERF Endometriosis Phenome and Biobanking Harmonisation Project for Experimental Models in Endometriosis Research (EPHect-EM-Pain): methods to assess pain behaviour in rodent models of endometriosis Open

Kelsi N. Dodds, Victor Fattori, Nick Andrews, Caroline B. Appleyard, Julie A. Christianson , et al. · 2025

Pain is a debilitating symptom of endometriosis, and its mechanisms are often explored using rodent models. However, a lack of harmonization amongst models and behavioural measures, in addition to inconsistent reporting, might limit the ov…

Key Considerations for Studying the Effects of High-Fat Diet on the Nulligravid Mouse Endometrium Open

Hilary Skalski, Amelia R Arendt, Shannon Harkins, Madison MacLachlan, Cody Corbett , et al. · 2024

The obesity epidemic continues to increase, with half of US women predicted to be obese by 2030. Women with obesity are at increased risk for not only cardiovascular and liver disease, but also reproductive disorders. Although mouse models…

PIK3CA mutation in endometriotic epithelial cells promotes viperin-dependent inflammatory response to insulin Open

Mike R. Wilson, Shannon Harkins, Jake J. Reske, Rebecca A. Siwicki, Marie Adams , et al. · 2023

Poster Presentations Open

Daniel Schemenauer, Dorothy Sojka, Anna Hu, Yuan You, Annie Thy Nguyen , et al. · 2023

Problem: Epidemiological studies in humans implicate maternal expression of different combinations of KIRs, which are a family of cell surface receptors that tune cellular activation status, as being predictive in pregnancy complication on…

Obesity alters the mouse endometrial transcriptome in a cell context-dependent manner Open

Mike R. Wilson, Hilary Skalski, Jake J. Reske, Marc Wegener, Marie Adams , et al. · 2022

ARID1A-dependent maintenance of H3.3 is required for repressive CHD4-ZMYND8 chromatin interactions at super-enhancers Open

Jake J. Reske, Mike R. Wilson, Brooke Armistead, Shannon Harkins, Cristina Pérez , et al. · 2022

Background SWI/SNF (BAF) chromatin remodeling complexes regulate lineage-specific enhancer activity by promoting accessibility for diverse DNA-binding factors and chromatin regulators. Additionally, they are known to modulate the function …

Transcriptome and DNA methylome analyses reveal underlying mechanisms for the racial disparity in uterine fibroids Open

Emmanuel N. Paul, Joshua A. Grey, Tyler J. Carpenter, Zachary Madaj, Kin H. Lau , et al. · 2022

Uterine fibroids (leiomyomas) affect Black women disproportionately compared with women of other races and ethnicities in terms of prevalence, incidence, and severity of symptoms. The causes of this racial disparity are essentially unknown…

Endometrial Epithelial ARID1A Is Required for Uterine Immune Homeostasis during Early Pregnancy Open

Ryan M. Marquardt, Soo Hyun Ahn, Jake J. Reske, Ronald L. Chandler, Margaret G. Petroff , et al. · 2022

A growing body of work suggests epigenetic dysregulation contributes to endometriosis pathophysiology and female infertility. The chromatin remodeling complex subunit AT-rich interaction domain 1A (ARID1A) must be properly expressed to mai…

AP-1 Subunit JUNB Promotes Invasive Phenotypes in Endometriosis Open

Mike R. Wilson, Jake J. Reske, Ronald L. Chandler · 2022

SWI/SNF Antagonism of PRC2 Mediates Estrogen-Induced Progesterone Receptor Expression Open

Mike R. Wilson, Jake J. Reske, Julie Koeman, Marie Adams, Niraj Joshi , et al. · 2022

Endometrial cancer (EC) is characterized by high estrogen levels unopposed by progesterone. Treatment with progestins is standard for early EC, but the response to progestins is dependent on progesterone receptor (PGR) expression. Here, we…

ARID1A maintains transcriptionally repressive H3.3 associated with CHD4-ZMYND8 chromatin interactions Open

Jake J. Reske, Mike R. Wilson, Brooke Armistead, Cristina Pérez, Joel Hrit , et al. · 2022

ARID1A is a signature subunit of the mammalian SWI/SNF (BAF) chromatin remodeling complex and is mutated at a high rate in malignancies and benign diseases originating from the uterine endometrium. Through genome-wide analysis of human end…

Additional file 2 of Obesity alters the mouse endometrial transcriptome in a cell context-dependent manner Open

Mike R. Wilson, Hilary Skalski, Jake J. Reske, Marc Wegener, Marie Adams , et al. · 2022

Additional file 2: Supplemental Data: Table 1. Differential Gene Expression.

Co-existing TP53 and ARID1A mutations promote aggressive endometrial tumorigenesis Open

Jake J. Reske, Mike R. Wilson, Jeanne Holladay, Rebecca A. Siwicki, Hilary Skalski , et al. · 2021

TP53 and ARID1A are frequently mutated across cancer but rarely in the same primary tumor. Endometrial cancer has the highest TP53-ARID1A mutual exclusivity rate. However, the functional relationship between TP53 and ARID1A mutations in th…

ARID1A Mutations Promote P300-Dependent Endometrial Invasion through Super-Enhancer Hyperacetylation Open

Mike R. Wilson, Jake J. Reske, Jeanne Holladay, Subechhya Neupane, Julie Ngo , et al. · 2020

SWI/SNF inactivation in the endometrial epithelium leads to loss of epithelial integrity Open

Jake J. Reske, Mike R. Wilson, Jeanne Holladay, Marc Wegener, Marie Adams , et al. · 2020

Although ARID1A mutations are a hallmark feature, mutations in other SWI/SNF (SWItch/Sucrose Non-Fermentable) chromatin remodeling subunits are also observed in endometrial neoplasms. Here, we interrogated the roles of Brahma/SWI2-related …

Lgr5-positive endothelial progenitor cells occupy a tumor and injury prone niche in the kidney vasa recta Open

Mike R. Wilson, Jeanne Holladay, Rachael Sheridan, Galen Hostetter, Bree D. Berghuis , et al. · 2020

ATAC-seq normalization method can significantly affect differential accessibility analysis and interpretation Open