Rosalie C. Sears
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View article: MicroCT enables simultaneous longitudinal tracking of murine pancreatic cancer progression and cachexia
MicroCT enables simultaneous longitudinal tracking of murine pancreatic cancer progression and cachexia Open
Preclinical models of pancreatic ductal adenocarcinoma (PDAC) can greatly benefit from noninvasive imaging for evaluating disease progression and therapeutic response. Imaging approaches that can accurately and simultaneously track primary…
View article: GNL3 SUMOylation is essential for DNA double-strand break repair by homologous recombination
GNL3 SUMOylation is essential for DNA double-strand break repair by homologous recombination Open
DNA double-strand break (DSB) repair via homologous recombination (HR) is critical for maintaining genomic integrity and requires proper DNA end resection to generate single-stranded DNA (ssDNA) overhangs. However, the mechanisms governing…
View article: 1250 Post-transcriptional regulator HuR promotes immune evasion in pancreatic ductal adenocarcinoma
1250 Post-transcriptional regulator HuR promotes immune evasion in pancreatic ductal adenocarcinoma Open
View article: Tumor-intrinsic MHC-II activation in pancreatic ductal adenocarcinoma enhances immune response and treatment efficacy
Tumor-intrinsic MHC-II activation in pancreatic ductal adenocarcinoma enhances immune response and treatment efficacy Open
Pancreatic ductal adenocarcinoma (PDAC) is characterized by an immunosuppressive tumor microenvironment (TME) and poor prognosis. While major histocompatibility complex class II (MHC-II) expression is traditionally associated with professi…
View article: Targeting KRAS Inhibitor–Resistant Pancreatic Cancer with an MUC1-C Antibody–Drug Conjugate
Targeting KRAS Inhibitor–Resistant Pancreatic Cancer with an MUC1-C Antibody–Drug Conjugate Open
Purpose: Treatment of pancreatic ductal adenocarcinoma (PDAC) has been advanced by the development of KRAS inhibitors. Despite this progress, PDAC invariably develop resistance to these agents through multiple mechanisms. In this study, we…
View article: Validation of a Serum-Based Biomarker Signature for Detection of Early-Stage Pancreatic Ductal Adenocarcinoma
Validation of a Serum-Based Biomarker Signature for Detection of Early-Stage Pancreatic Ductal Adenocarcinoma Open
These findings indicate that PancreaSure is a high-performing biomarker test to aid in the detection of early-stage PDAC. Overall, this work represents an important step toward improving early-stage diagnostic success.
View article: S3R: Modeling spatially varying associations with Spatially Smooth Sparse Regression
S3R: Modeling spatially varying associations with Spatially Smooth Sparse Regression Open
Spatial transcriptomics (ST) data demands models that recover how associations among molecular and cellular features change across tissue while contending with noise, collinearity, cell mixing, and thousands of predictors. We present Spati…
View article: Supplementary Figure S6 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer
Supplementary Figure S6 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer Open
EV injected into mice are imported by cells in PDAC tumors.
View article: Supplementary Data 3 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer
Supplementary Data 3 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer Open
Supplementary Data 3. HPaMECs treated with WT vs. HuR KO EVs
View article: Supplementary Table S4 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer
Supplementary Table S4 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer Open
Flow cytometry gating strategy for orthotopic pancreatic tumors
View article: Supplementary Figure S4 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer
Supplementary Figure S4 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer Open
Additional mouse model staining.
View article: Supplementary Figure S3 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer
Supplementary Figure S3 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer Open
Repeating in vitro EV treatments with an additional endothelial cell line.
View article: Figure 5 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer
Figure 5 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer Open
Tumor-intrinsic HuR directly alters endothelial cell function via EV signaling. A, Schematic of the PalmGRET reporter labeling the inner leaflet of all membranes with GFP-nLuc via integration of a palmitoylation sequence. The constr…
View article: Figure 4 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer
Figure 4 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer Open
Tumor-intrinsic HuR promotes endothelial cell recruitment and sprouting in PDAC tumors. A, Immunoblot of KPC WT and HuR-KO clones 6, 8, and 9 probed for HuR and loading control vinculin. B, KPC WT and HuR-KO cells were implan…
View article: Supplementary Figure S5 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer
Supplementary Figure S5 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer Open
Validation of PalmGRET reporter in KPC cells.
View article: Supplementary Figure S2 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer
Supplementary Figure S2 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer Open
Validation of EV import imaging in vitro.
View article: Figure 1 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer
Figure 1 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer Open
HuR affects PDAC EV cargoes relating to endothelial cell functions. A, Schematic of EV isolation from PANC-1 WT vs. HuR-KO cells for EV validation, RNA-seq, and proteomics. B, Immunoblot of cell lysates (CL) and SEC fractions…
View article: Supplementary Table S3 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer
Supplementary Table S3 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer Open
Flow cytometry antibody information
View article: Supplementary Table S2 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer
Supplementary Table S2 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer Open
Immunofluorescence antibody information
View article: Supplementary Data 2 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer
Supplementary Data 2 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer Open
Supplementary Data 2. Proteomic data from WT vs. HuR KO EV cargo
View article: Figure 3 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer
Figure 3 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer Open
WT EVs directly alter endothelial cell transcriptome, migration, barrier function, and tube formation. A, HPaMECs were treated with WT vs. HuR-KO EVs for 24 hours before RNA-seq and phenotypic assessment of migration, monolayer perm…
View article: Supplementary Data 1 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer
Supplementary Data 1 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer Open
Supplementary Data 1. WT vs. HuR KO EV cargo
View article: Supplementary Figure S1 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer
Supplementary Figure S1 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer Open
PANC-1 HuR KO and EV isolation and phospho-proteomics.
View article: Data from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer
Data from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer Open
Pancreatic ductal adenocarcinoma (PDAC) tumors are hypovascular with collapsed and dysfunctional vessels that limit immunosurveillance and contribute to early metastatic events. However, current antiangiogenic therapies have failed in PDAC…
View article: Figure 2 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer
Figure 2 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer Open
Endothelial cell abundance correlates with ELAVL1 expression, and endothelial cells import PDAC EVs. A, Correlation between ELAVL1 expression in PDAC cells and the presence of endothelial cells in publicly available pa…
View article: Supplementary Table S1 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer
Supplementary Table S1 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer Open
Immunoblotting antibody information
View article: Figure 6 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer
Figure 6 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer Open
HuR WT EVs are tumor promoting and directly decrease ICAM-1 expression on endothelial cells. A, Schematic of EV isolation from KPC WT and HuR-KO cells via SEC. B, Immunoblot of cell lysates (CL) and SEC fractions 1, 7–10 (EV …
View article: HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer
HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer Open
Pancreatic ductal adenocarcinoma (PDAC) tumors are hypovascular with collapsed and dysfunctional vessels that limit immunosurveillance and contribute to early metastatic events. However, current antiangiogenic therapies have failed in PDAC…
View article: Cell free RNA detection of pancreatic cancer in pre diagnostic high risk and symptomatic patients
Cell free RNA detection of pancreatic cancer in pre diagnostic high risk and symptomatic patients Open
View article: Myc and Kras cooperate in adult acinar cells to drive phenotypic heterogeneity, metastasis, and therapeutic resistance in a novel pancreatic cancer mouse model
Myc and Kras cooperate in adult acinar cells to drive phenotypic heterogeneity, metastasis, and therapeutic resistance in a novel pancreatic cancer mouse model Open
Pancreatic ductal adenocarcinoma (PDAc) is a deadly malignancy, most commonly diagnosed in advanced stages when no curative treatments are available. The development of new models that aid ongoing investigation into the mechanisms by which…