Roy Spijkerman
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View article: Identification of neutrophil phenotype categories in geriatric hip fracture patients aids in personalized medicine
Identification of neutrophil phenotype categories in geriatric hip fracture patients aids in personalized medicine Open
Objectives: The number of geriatric hip fracture patients is high and expected to rise in the coming years, and many are frail and at risk for adverse outcomes. Early identification of high-risk patients is crucial to balance treatment and…
View article: Shift of Neutrophils From Blood to Bone Marrow Upon Extensive Experimental Trauma Surgery
Shift of Neutrophils From Blood to Bone Marrow Upon Extensive Experimental Trauma Surgery Open
Introduction Extensive trauma surgery evokes an immediate cellular immune response including altered circulatory neutrophil numbers. The concurrent bone marrow (BM) response however is currently unclear. We hypothesize that these BM change…
View article: Comprehensive multivariate evaluation of the effects on cell phenotypes in multicolor flow cytometry data using ANOVA simultaneous component analysis
Comprehensive multivariate evaluation of the effects on cell phenotypes in multicolor flow cytometry data using ANOVA simultaneous component analysis Open
This work proposes an approach to assess the effects observed in multicolor flow cytometry (MFC) experiments, for all markers and experimental factors simultaneously. It achieves this end by extending ANOVA simultaneous component analysis …
View article: Point-of-care fully automated flow cytometry
Point-of-care fully automated flow cytometry Open
This thesis describes the introduction of a new method for bedside analysis of innate immune cells in health and disease. Flow cytometry is an analysis method, that is almost 50 years old and has been extensively used in biomedical researc…
View article: Kinetics of Neutrophil Subsets in Acute, Subacute, and Chronic Inflammation
Kinetics of Neutrophil Subsets in Acute, Subacute, and Chronic Inflammation Open
At homeostasis the vast majority of neutrophils in the circulation expresses CD16 and CD62L within a narrow expression range, but this quickly changes in disease. Little is known regarding the changes in kinetics of neutrophils phenotypes …
View article: The Systemic Immune Response in COVID-19 Is Associated with a Shift to Formyl-Peptide Unresponsive Eosinophils
The Systemic Immune Response in COVID-19 Is Associated with a Shift to Formyl-Peptide Unresponsive Eosinophils Open
A malfunction of the innate immune response in COVID-19 is associated with eosinopenia, particularly in more severe cases. This study tested the hypothesis that this eosinopenia is COVID-19 specific and is associated with systemic activati…
View article: Thrombotic Events in COVID-19 Are Associated With a Lower Use of Prophylactic Anticoagulation Before Hospitalization and Followed by Decreases in Platelet Reactivity
Thrombotic Events in COVID-19 Are Associated With a Lower Use of Prophylactic Anticoagulation Before Hospitalization and Followed by Decreases in Platelet Reactivity Open
Background: Coronavirus disease of 2019 (COVID-19) is associated with a prothrombotic state and a high incidence of thrombotic event(s) (TE). Objectives: To study platelet reactivity in hospitalized COVID-19 patients and determine a possib…
View article: Refractory neutrophils and monocytes in patients with inflammatory bowel disease after repeated bouts of prolonged exercise
Refractory neutrophils and monocytes in patients with inflammatory bowel disease after repeated bouts of prolonged exercise Open
Background Neutrophils and monocytes are key immune effector cells in inflammatory bowel disease (IBD) that is associated with chronic inflammation in the gut. Patients with stable IBD who perform exercise have significantly fewer flare‐up…
View article: An increase in CD62L<sup>dim</sup> neutrophils precedes the development of pulmonary embolisms in COVID‐19 patients
An increase in CD62L<sup>dim</sup> neutrophils precedes the development of pulmonary embolisms in COVID‐19 patients Open
Objectives A high incidence of pulmonary embolism (PE) is reported in patients with critical coronavirus disease 2019 (COVID‐19). Neutrophils may contribute to this through a process referred to as immunothrombosis. The aim of this study w…
View article: Flow cytometric evaluation of the neutrophil compartment in COVID-19 at hospital presentation: A normal response to an abnormal situation
Flow cytometric evaluation of the neutrophil compartment in COVID-19 at hospital presentation: A normal response to an abnormal situation Open
Coronavirus disease 2019 (COVID-19) is a rapidly emerging pandemic disease caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Critical COVID-19 is thought to be associated with a hyper-inflammatory process that can…
View article: Analysis of human neutrophil phenotypes as biomarker to monitor exercise-induced immune changes
Analysis of human neutrophil phenotypes as biomarker to monitor exercise-induced immune changes Open
The amplitude of the innate immune response reflects the degree of physiological stress imposed by exercise load. An optimal balance of exercise intensity and duration is essential for a balanced immune system and reduces the risk of dysfu…
View article: Refractory neutrophils and monocytes in patients with inflammatory bowel disease after repeated bouts of prolonged exercise
Refractory neutrophils and monocytes in patients with inflammatory bowel disease after repeated bouts of prolonged exercise Open
Rationale Neutrophils and monocytes are key immune effector cells in inflammatory bowel disease (IBD) that is associated with chronic inflammation in the gut. Patients with stable IBD who exercise have fewer flare-ups, but no underlying me…
View article: Fragile neutrophils in surgical patients: A phenomenon associated with critical illness
Fragile neutrophils in surgical patients: A phenomenon associated with critical illness Open
Leukocyte viability (determined by e.g. propidium iodide [PI] staining) is automatically measured by hematology analyzers to check for delayed bench time. Incidental findings in fresh blood samples revealed the existence of leukocytes with…
View article: Point-of-Care Analysis of Neutrophil Phenotypes: A First Step Toward Immuno-Based Precision Medicine in the Trauma ICU
Point-of-Care Analysis of Neutrophil Phenotypes: A First Step Toward Immuno-Based Precision Medicine in the Trauma ICU Open
Objectives: The amount of tissue damage and the amplitude of the immune response after trauma are related to the development of infectious complications later on. Changes in the neutrophil compartment can be used as read out of the amplitu…
View article: Splenectomy is Associated with Altered Leukocyte Kinetics after Severe Trauma
Splenectomy is Associated with Altered Leukocyte Kinetics after Severe Trauma Open
Background Inadequate activation of the innate immune system after trauma can lead to severe complications such as Acute Respiratory Distress Syndrome and Multiple Organ Dysfunction Syndrome. The spleen is thought to modulate the cellular …
View article: New automated analysis to monitor neutrophil function point-of-care in the intensive care unit after trauma
New automated analysis to monitor neutrophil function point-of-care in the intensive care unit after trauma Open
Background Patients often develop infectious complications after severe trauma. No biomarkers exist that enable early identification of patients who are at risk. Neutrophils are important immune cells that combat these infections by phagoc…
View article: Persistent Inflammation, Immunosuppression and Catabolism Syndrome (PICS) after Polytrauma: A Rare Syndrome with Major Consequences
Persistent Inflammation, Immunosuppression and Catabolism Syndrome (PICS) after Polytrauma: A Rare Syndrome with Major Consequences Open
Nowadays, more trauma patients develop chronic critical illness (CCI), a state characterized by prolonged intensive care. Some of these CCI patients have disproportional difficulties to recover and suffer from recurrent infections, a syndr…
View article: Automated flow cytometry enables high performance point-of-care analysis of leukocyte phenotypes
Automated flow cytometry enables high performance point-of-care analysis of leukocyte phenotypes Open
This study showed that bench time of one hour already leads to a significant upregulation and bigger variance in activation markers of granulocytes and monocytes. In addition, it is likely that automated flow cytometry reduces intra-assay …
View article: A cohort study to evaluate infection prevention protocol in pediatric trauma patients with blunt splenic injury in a Dutch level 1 trauma center
A cohort study to evaluate infection prevention protocol in pediatric trauma patients with blunt splenic injury in a Dutch level 1 trauma center Open
The vaccination status, the level of knowledge concerning prevention of an OPSI, and the use of prophylactic antibiotics are suboptimal in pediatric patients treated for BSI. Therefore, we created a new follow-up treatment guideline to hav…