Ruby E. Harrison
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View article: Protocol for laboratory rearing and infection tracking of Rhodnius prolixus using 3D-printable designs
Protocol for laboratory rearing and infection tracking of Rhodnius prolixus using 3D-printable designs Open
Human infections by Trypanosoma cruzi propagate via its blood-feeding triatomine vector. Investigating parasite-vector interactions depends upon robust techniques to rear insects and analyze infections. Here, we present a protocol for labo…
View article: Stable colonization of the kissing bug Rhodnius prolixus by Trypanosoma cruzi Y strain
Stable colonization of the kissing bug Rhodnius prolixus by Trypanosoma cruzi Y strain Open
Trypanosoma cruzi is a single-celled eukaryotic parasite responsible for Chagas disease, a major cause of morbidity and mortality in Central and South America. While the host-pathogen interactions of T. cruzi have been extensively studied …
View article: A limitation lifted: A conditional knockdown system reveals essential roles for Polo-like kinase and Aurora kinase 1 in <i>Trypanosoma cruzi</i> cell division
A limitation lifted: A conditional knockdown system reveals essential roles for Polo-like kinase and Aurora kinase 1 in <i>Trypanosoma cruzi</i> cell division Open
While advances in genome editing technologies have simplified gene disruption in many organisms, the study of essential genes requires development of conditional disruption or knockdown systems that are not available in most organisms. Suc…
View article: The mosquito Aedes aegypti requires a gut microbiota for normal fecundity, longevity and vector competence
The mosquito Aedes aegypti requires a gut microbiota for normal fecundity, longevity and vector competence Open
Mosquitoes shift from detritus-feeding larvae to blood-feeding adults that can vector pathogens to humans and other vertebrates. The sugar and blood meals adults consume are rich in carbohydrates and protein but are deficient in other nutr…
View article: Riboflavin instability is a key factor underlying the requirement of a gut microbiota for mosquito development
Riboflavin instability is a key factor underlying the requirement of a gut microbiota for mosquito development Open
Significance Mosquitoes are major disease vectors that require a gut microbiota for development into adults under some conditions but not others. To determine the underlying mechanisms for this variation, we determined that dietary ribofla…
View article: Whole blood and blood components from vertebrates differentially affect egg formation in three species of anautogenous mosquitoes
Whole blood and blood components from vertebrates differentially affect egg formation in three species of anautogenous mosquitoes Open
Background Most female mosquitoes are anautogenous and must blood feed on a vertebrate host to produce eggs. Prior studies show that the number of eggs females lay per clutch correlates with the volume of blood ingested and that protein is…
View article: Both living bacteria and eukaryotes in the mosquito gut promote growth of larvae
Both living bacteria and eukaryotes in the mosquito gut promote growth of larvae Open
We recently reported that larval stage Aedes aegypti and several other species of mosquitoes grow when living bacteria are present in the gut but do not grow when living bacteria are absent. We further reported that living bacteria induce …