Ruiming Wang
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View article: Intracellular self-assembly and metabolite analysis of key enzymes for L-lysine synthesis based on key components of cellulosomes
Intracellular self-assembly and metabolite analysis of key enzymes for L-lysine synthesis based on key components of cellulosomes Open
Introduction Cellulosome is a natural multi-enzyme complex in the extracellular space of anaerobic microorganisms, which has the advantages of small molecular weight, multiple binding sites, and strong designability. This study aimed to ex…
View article: Enhancing 10-HDA production of Escherichia coli by heterologous expression of MexHID transporter proteins
Enhancing 10-HDA production of Escherichia coli by heterologous expression of MexHID transporter proteins Open
10-Hydroxy-2-decenoic acid (10-HDA) is a medium-chain α,β-unsaturated carboxylic acid that exists in royal jelly with terminal hydroxylation. It has a broad market value because of its antibacterial, anti-inflammatory, anti-tumor, anti-rad…
View article: Proteomics mechanism and clinical efficacy of Jujuboside A: a Meta-analysis
Proteomics mechanism and clinical efficacy of Jujuboside A: a Meta-analysis Open
View article: Combining Computer-Aided Enzyme Design and Chromosomal Integration for Plasmid-Free Biosynthesis of 1,5-Pentanediol in Escherichia Coli
Combining Computer-Aided Enzyme Design and Chromosomal Integration for Plasmid-Free Biosynthesis of 1,5-Pentanediol in Escherichia Coli Open
View article: Photoinduced Oxidation of Benzylic Hydrogens Using N-Bromosuccinimide
Photoinduced Oxidation of Benzylic Hydrogens Using N-Bromosuccinimide Open
View article: Silk Protein Gene Engineering and Its Applications: Recent Advances in Biomedicine Driven by Molecular Biotechnology
Silk Protein Gene Engineering and Its Applications: Recent Advances in Biomedicine Driven by Molecular Biotechnology Open
Silk protein, as a natural polymer material with unique structures and properties, exhibits tremendous potential in the biomedical field. Given the limited production and restricted properties of natural silk proteins, molecular biotechnol…
View article: Proline Improves Pullulan Biosynthesis Under High Sugar Stress Condition
Proline Improves Pullulan Biosynthesis Under High Sugar Stress Condition Open
Pullulan is an extracellular polysaccharide produced via the fermentation of Aureobasidium pullulans. However, high sugar concentrations and hyperosmotic stress limit pullulan biosynthesis during the fermentation process. Therefore, we inv…
View article: Serum Lipoprotein(a) as Predictive Factor for Early Neurological Deterioration of Acute Ischemic Stroke After Thrombolysis
Serum Lipoprotein(a) as Predictive Factor for Early Neurological Deterioration of Acute Ischemic Stroke After Thrombolysis Open
Our findings demonstrated that LP(a) was an independent risk factor for END post-thrombolysis and that LP(a) level≥ 300 mg/L could be associated with END post-thrombolysis in this study population.
View article: Safety and efficacy evaluation of halicin as an effective drug for inhibiting intestinal infections
Safety and efficacy evaluation of halicin as an effective drug for inhibiting intestinal infections Open
Halicin, the first antibacterial agent discovered by artificial intelligence, exerts broad-spectrum antibacterial effects and has a unique structure. Our study found that halicin had a good inhibitory effect on clinical isolates of drug-re…
View article: Improving the Synthesis Efficiency of Amino Acids by Analyzing the Key Sites of Intracellular Self-Assembly of Artificial Cellulosome
Improving the Synthesis Efficiency of Amino Acids by Analyzing the Key Sites of Intracellular Self-Assembly of Artificial Cellulosome Open
To explore the key sites affecting the intracellular assembly of key components of cellulosomes and obtain DocA mutants independent of Ca2+, Swiss-model, GROMACS, PyMOL, and other molecular dynamics simulation software were used for modeli…
View article: Key enzymes involved in the utilization of fatty acids by Saccharomyces cerevisiae: a review
Key enzymes involved in the utilization of fatty acids by Saccharomyces cerevisiae: a review Open
Saccharomyces cerevisiae is a eukaryotic organism with a clear genetic background and mature gene operating system; in addition, it exhibits environmental tolerance. Therefore, S. cerevisiae is one of the most commonly used organisms for t…
View article: <scp>DNA</scp> methylation remodeled amino acids biosynthesis regulates flower senescence in carnation (<i>Dianthus caryophyllus</i>)
<span>DNA</span> methylation remodeled amino acids biosynthesis regulates flower senescence in carnation (<i>Dianthus caryophyllus</i>) Open
Summary Dynamic DNA methylation regulatory networks are involved in many biological processes. However, how DNA methylation patterns change during flower senescence and their relevance with gene expression and related molecular mechanism r…
View article: Enhancing the erythritol production of <i>Yarrowia lipolytica</i> by high-throughput screening based on highly sensitive artificial sensor and anchor protein cwp2
Enhancing the erythritol production of <i>Yarrowia lipolytica</i> by high-throughput screening based on highly sensitive artificial sensor and anchor protein cwp2 Open
Yarrowia lipolytica is widely used for the industrial production of the natural sweetener erythritol. Despite improvements in fermentation process control and metabolic pathway regulation, bottlenecks still exist in terms of yield and scre…
View article: Construction and Characterization of Fitting Equations for a New Wheat Straw Pulping Method
Construction and Characterization of Fitting Equations for a New Wheat Straw Pulping Method Open
The pretreatment of pulp with enzymes has been extensively studied in the laboratory. However, due to cost constraints, the application of enzymes in the pulp and paper industry is very limited. In this paper, an environment-friendly and e…
View article: Sustainable Utilization of Pulp and Paper Wastewater
Sustainable Utilization of Pulp and Paper Wastewater Open
The pulp and paper industry plays an important role in the global economy and is inextricably linked to human life. Due to its large scale, the production process generates a large amount of wastewater, which poses a major threat to the en…
View article: Development of Raw Materials and Technology for Pulping—A Brief Review
Development of Raw Materials and Technology for Pulping—A Brief Review Open
Paper is one of the most significant inventions in human civilization, which considerably advanced global cultural development. Pulping is a key step in the conversion of fiber raw materials into paper. Since its inception, pulping has rap…
View article: 10-hydroxy-2-decenoic acid alleviates lipopolysaccharide-induced intestinal mucosal injury through anti-inflammatory, antioxidant, and gut microbiota modulation activities in chickens
10-hydroxy-2-decenoic acid alleviates lipopolysaccharide-induced intestinal mucosal injury through anti-inflammatory, antioxidant, and gut microbiota modulation activities in chickens Open
Introduction This study aimed to investigated the effects of 10-hydroxy-2-decenoic acid (10-HDA) on the growth performance, intestinal barrier, inflammatory response, oxidative stress, and gut microbiota of chickens challenged with lipopol…
View article: Screening l-Lysine-Overproducing Escherichia coli Using Artificial Rare Codons and a Rare Codon-Rich Marker
Screening l-Lysine-Overproducing Escherichia coli Using Artificial Rare Codons and a Rare Codon-Rich Marker Open
l-Lysine, an essential amino acid for humans and mammals, is widely used in the food, feed, medicine, and cosmetics industries. In this study, a lysine over-producing Escherichia coli mutant was isolated using a fluorescence-based screen a…
View article: A specific and rapid method for detecting Bacillus and Acinetobacter species in Daqu
A specific and rapid method for detecting Bacillus and Acinetobacter species in Daqu Open
Daqu is a spontaneous, solid-state cereal fermentation product used for saccharification and as a starter culture for Chinese Baijiu production. Bacillus and Acinetobacter , two dominant microbial genera in Daqu, produce enzymes and organi…
View article: Flow-cytometric cell sorting coupled with UV mutagenesis for improving pectin lyase expression
Flow-cytometric cell sorting coupled with UV mutagenesis for improving pectin lyase expression Open
Introduction: Alkaline pectin lyase is an important enzyme with a wide range of applications in industrial production, It has been widely used in many important fields such as fruit juice processing and extraction, the dyeing and processin…
View article: An insertion of transposon in <i>DcNAP</i> inverted its function in the ethylene pathway to delay petal senescence in carnation (<i>Dianthus caryophyllus</i> L.)
An insertion of transposon in <i>DcNAP</i> inverted its function in the ethylene pathway to delay petal senescence in carnation (<i>Dianthus caryophyllus</i> L.) Open
Summary Petal senescence is the final stage of flower development. Transcriptional regulation plays key roles in this process. However, whether and how post‐transcriptional regulation involved is still largely unknown. Here, we identified …
View article: Structural Characterization and Antifatigue Activity of a Novel Exopolysaccharide Isolated from Marasmius androsaceus
Structural Characterization and Antifatigue Activity of a Novel Exopolysaccharide Isolated from Marasmius androsaceus Open
This study aimed at examining the structure-role modeling and antifatigue mechanism of polysaccharides, including M. androsaceus exopolysaccharide 3 (MEPS3), isolated from Marasmius androsaceus fermentation broth. The molecular weight of M…
View article: Improving the regeneration rate of deep lethal mutant protoplasts by fusion to promote efficient L-lysine fermentation
Improving the regeneration rate of deep lethal mutant protoplasts by fusion to promote efficient L-lysine fermentation Open
View article: Relationship between Representative Trace Components and Health Functions of Chinese Baijiu: A Review
Relationship between Representative Trace Components and Health Functions of Chinese Baijiu: A Review Open
Baijiu is a traditional fermented Chinese beverage with a history of hundreds of years. The notable characteristics of Baijiu include diverse raw materials, complex technology, and the co-fermentation of multiple strains. Complex brewing t…
View article: Improvement of optimum pH and specific activity of pectate lyase from Bacillus RN.1 using loop replacement
Improvement of optimum pH and specific activity of pectate lyase from Bacillus RN.1 using loop replacement Open
Background: Alkaline pectate lyase plays an important role in papermaking, biological refining and wastewater treatment, but its industrial applications are largely limited owing to its low activity and poor alkali resistance. Methods: The…
View article: Biobased polyesters and crosslinked polyester films derived from 10-hydroxy-2-decenoic acid: Synthesis and characterization
Biobased polyesters and crosslinked polyester films derived from 10-hydroxy-2-decenoic acid: Synthesis and characterization Open
In this study, we optimized the melt polycondensation of 10-hydroxy-2-decenoic acid (10-HDA) to produce poly(10-hydroxy-2-decenoate) (PHDA) and used it to form crosslinked polyester films of PHDA via thermal- or photocuring. The physical p…
View article: Study on the construction technology of β-alanine synthesizing Escherichia coli based on cellulosome assembly
Study on the construction technology of β-alanine synthesizing Escherichia coli based on cellulosome assembly Open
Introduction: β-Alanine is the only β-amino acid in nature; it is widely used in food additives, medicines, health products, and surfactants. To avoid pollution caused by traditional production methods, the synthesis of β-alanine has been …
View article: Biosynthesis and genetic engineering of phenazine-1-carboxylic acid in Pseudomonas chlororaphis Lzh-T5
Biosynthesis and genetic engineering of phenazine-1-carboxylic acid in Pseudomonas chlororaphis Lzh-T5 Open
Phenazine-1-carboxylic acid (PCA) is a biologically active substance with the ability to prevent and control crop diseases. It was certified as a pesticide by the Ministry of Agriculture of China in 2011 and was named “Shenzimycin.” Lzh-T5…
View article: Exploration of microbiome diversity of stacked fermented grains by flow cytometry and cell sorting
Exploration of microbiome diversity of stacked fermented grains by flow cytometry and cell sorting Open
Sauce-flavor baijiu is one of the twelve flavor types of Chinese distilled fermented product. Microbial composition plays a key role in the stacked fermentation of Baijiu, which uses grains as raw materials and produces flavor compounds, h…
View article: Rational Design of Disulfide Bonds Increases Thermostability of a β-1,3-1,4-Glucanase from Paenibacillus
Rational Design of Disulfide Bonds Increases Thermostability of a β-1,3-1,4-Glucanase from Paenibacillus Open
β-1,3 − 1,4-gluconases can specifically hydrolyze the adjacent β-1,4 glycoside bond of β-1,3 in β-glucan, which is widely used in food, brewing and feed industries. Its sources include bacteria, fungi, and plant endosperm cell walls, most …