Rūta Gerasimaitė
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View article: Biochemical and cellular insights into the Baz2B protein, a non-catalytic subunit of the chromatin remodeling complex
Biochemical and cellular insights into the Baz2B protein, a non-catalytic subunit of the chromatin remodeling complex Open
Baz2B is a regulatory subunit of the ATP-dependent chromatin remodeling complexes BRF1 and BRF5, which control access to DNA during DNA-templated processes. Baz2B has been implicated in several diseases and also in unhealthy ageing, howeve…
View article: Force generation in human blood platelets by filamentous actomyosin structures
Force generation in human blood platelets by filamentous actomyosin structures Open
Blood platelets are central elements of the blood clotting response after wounding. Upon vessel damage, they bind to the surrounding matrix and contract the forming thrombus, thus helping to restore normal blood circulation. The hemostatic…
View article: Inositol pyrophosphates activate the vacuolar transport chaperone complex in yeast by disrupting a homotypic SPX domain interaction
Inositol pyrophosphates activate the vacuolar transport chaperone complex in yeast by disrupting a homotypic SPX domain interaction Open
View article: Sequence-specific DNA labelling for fluorescence microscopy
Sequence-specific DNA labelling for fluorescence microscopy Open
The preservation of nucleus structure during microscopy imaging is a top priority for understanding chromatin organization, genome dynamics, and gene expression regulation. In this review, we summarize the sequence-specific DNA labelling m…
View article: A general highly efficient synthesis of biocompatible rhodamine dyes and probes for live-cell multicolor nanoscopy
A general highly efficient synthesis of biocompatible rhodamine dyes and probes for live-cell multicolor nanoscopy Open
The development of live-cell fluorescence nanoscopy is powered by the availability of suitable fluorescent probes. Rhodamines are among the best fluorophores for labeling intracellular structures. Isomeric tuning is a powerful method for o…
View article: A general, highly efficient and facile synthesis of biocompatible rhodamine dyes and probes for live-cell multicolor nanoscopy
A general, highly efficient and facile synthesis of biocompatible rhodamine dyes and probes for live-cell multicolor nanoscopy Open
The development of live-cell fluorescence nanoscopy is powered by the availability of suitable fluorescent probes. Rhodamines are among the best fluorophores for labeling intracellular structures. Isomeric tuning is a powerful method for o…
View article: Blinking Fluorescent Probes for Tubulin Nanoscopy in Living and Fixed Cells
Blinking Fluorescent Probes for Tubulin Nanoscopy in Living and Fixed Cells Open
Here we report a small molecule tubulin probe for single-molecule localization microscopy (SMLM), stimulated emission depletion (STED) microscopy and MINFLUX nanoscopy, which can be used in living and fixed cells. We explored a series of t…
View article: Inside a Shell—Organometallic Catalysis Inside Encapsulin Nanoreactors
Inside a Shell—Organometallic Catalysis Inside Encapsulin Nanoreactors Open
Compartmentalization of chemical reactions inside cells are a fundamental requirement for life. Encapsulins are self‐assembling protein‐based nanocompartments from the prokaryotic repertoire that present a highly attractive platform for in…
View article: Inside a Shell—Organometallic Catalysis Inside Encapsulin Nanoreactors
Inside a Shell—Organometallic Catalysis Inside Encapsulin Nanoreactors Open
Compartmentalization of chemical reactions inside cells are a fundamental requirement for life. Encapsulins are self‐assembling protein‐based nanocompartments from the prokaryotic repertoire that present a highly attractive platform for in…
View article: Blinking fluorescent probes for tubulin nanoscopy in living and fixed cells
Blinking fluorescent probes for tubulin nanoscopy in living and fixed cells Open
Here we report a small molecule probe for single molecule localisation microscopy (SMLM) of tubulin in living and fixed cells. We explored a series of constructs composed of taxanes and spontaneously blinking far-red dye hydroxymethyl sili…
View article: Direct Visualization of Amlodipine Intervention into Living Cells by Means of Fluorescence Microscopy
Direct Visualization of Amlodipine Intervention into Living Cells by Means of Fluorescence Microscopy Open
Amlodipine, a unique long-lasting calcium channel antagonist and antihypertensive drug, has weak fluorescence in aqueous solutions. In the current paper, we show that direct visualization of amlodipine in live cells is possible due to the …
View article: Enhancing biocompatibility of rhodamine fluorescent probes by a neighbouring group effect
Enhancing biocompatibility of rhodamine fluorescent probes by a neighbouring group effect Open
Fluorescence microscopy is an essential tool for understanding dynamic processes in living cells and organisms. However, many fluorescent probes for labelling cellular structures suffer from unspecific interactions and low cell permeabilit…
View article: Overcoming efflux of fluorescent probes for actin imaging in living cells
Overcoming efflux of fluorescent probes for actin imaging in living cells Open
Actin cytoskeleton is crucial for endocytosis, intracellular trafficking, cell shape maintenance and a wide range of other cellular functions. Recently introduced cell-permeable fluorescent actin probes suffer from poor membrane permeabili…
View article: Enhancing the biocompatibility of rhodamine fluorescent probes by a neighbouring group effect
Enhancing the biocompatibility of rhodamine fluorescent probes by a neighbouring group effect Open
Excellent live-cell staining and nanoscopy imaging with rhodamine 4′-isomer probes boosted by a neighboring group effect.
View article: Efflux pump insensitive rhodamine–jasplakinolide conjugates for G- and F-actin imaging in living cells
Efflux pump insensitive rhodamine–jasplakinolide conjugates for G- and F-actin imaging in living cells Open
Fluorescent actin probes made of 6′-carbopyronines and jasplakinolide are insensitive to efflux pumps and stain F- and G-actin efficiently in living cells, allowing high quality 2D and 3D nanoscopy of dynamic actin structures.
View article: Application of STED imaging for chromatin studies
Application of STED imaging for chromatin studies Open
Chromatin is the information center of a cell. It comprises proteins and nucleic acids that form a highly complex and dynamic structure within the nucleus. Its multiple organization levels span from micrometre to nanometre scale. For many …
View article: Excision of the doubly methylated base <i>N</i> <sup>4</sup> ,5-dimethylcytosine from DNA by <i>Escherichia coli</i> Nei and Fpg proteins
Excision of the doubly methylated base <i>N</i> <sup>4</sup> ,5-dimethylcytosine from DNA by <i>Escherichia coli</i> Nei and Fpg proteins Open
Cytosine (C) in DNA is often modified to 5-methylcytosine (m 5 C) to execute important cellular functions. Despite the significance of m 5 C for epigenetic regulation in mammals, damage to m 5 C has received little attention. For instance,…
View article: The Use of Hoechst Dyes for DNA Staining and Beyond
The Use of Hoechst Dyes for DNA Staining and Beyond Open
Hoechst dyes are among the most popular fluorophores used to stain DNA in living and fixed cells. Moreover, their high affinity and specificity towards DNA make Hoechst dyes excellent targeting moieties, which can be conjugated to various …
View article: Supplementary material from "Excision of the doubly methylated base <i>N</i>4,5-dimethylcytosine from DNA by <i>Escherichia coli</i> Nei and Fpg proteins"
Supplementary material from "Excision of the doubly methylated base <i>N</i>4,5-dimethylcytosine from DNA by <i>Escherichia coli</i> Nei and Fpg proteins" Open
Cytosine (C) in DNA is often modified to 5-methylcytosine (m5C) to execute important cellular functions. Despite the significance of m5C for epigenetic regulation in mammals, damage to m5C has received litt…
View article: Ppn2, a novel Zn2+-dependent polyphosphatase in the acidocalcisome-like yeast vacuole
Ppn2, a novel Zn2+-dependent polyphosphatase in the acidocalcisome-like yeast vacuole Open
Acidocalcisome-like organelles are found in all kingdoms of life. Many of their functions, such as the accumulation and storage of metal ions, nitrogen and phosphate, the activation of blood clotting and inflammation, depend on the control…
View article: Inositol Pyrophosphate Specificity of the SPX-Dependent Polyphosphate Polymerase VTC
Inositol Pyrophosphate Specificity of the SPX-Dependent Polyphosphate Polymerase VTC Open
The free energy of nucleotide hydrolysis depends on phosphate concentration. Cells regulate cytosolic phosphate levels by orchestrating phosphate acquisition and storage through inositol pyrophosphates (PP-InsP) and SPX domains. Here, we r…
View article: Vtc5, a Novel Subunit of the Vacuolar Transporter Chaperone Complex, Regulates Polyphosphate Synthesis and Phosphate Homeostasis in Yeast
Vtc5, a Novel Subunit of the Vacuolar Transporter Chaperone Complex, Regulates Polyphosphate Synthesis and Phosphate Homeostasis in Yeast Open
SPX domains control phosphate homeostasis in eukaryotes. Ten genes in yeast encode SPX-containing proteins, among which YDR089W is the only one of unknown function. Here, we show that YDR089W encodes a novel subunit of the vacuole transpor…
View article: Organelle acidification negatively regulates vacuole membrane fusion in vivo
Organelle acidification negatively regulates vacuole membrane fusion in vivo Open
View article: Control of eukaryotic phosphate homeostasis by inositol polyphosphate sensor domains
Control of eukaryotic phosphate homeostasis by inositol polyphosphate sensor domains Open
Peptide domain links phosphate need to uptake Cellular phosphate (Pi) levels are tightly controlled, but it is not clear how eukaryotic cells actually “measure” the concentration of Pi. Wild et al. now show that inositol polyphosphate (Ins…
View article: Enzymes of yeast polyphosphate metabolism: structure, enzymology and biological roles
Enzymes of yeast polyphosphate metabolism: structure, enzymology and biological roles Open
Inorganic polyphosphate (polyP) is found in all living organisms. The known polyP functions in eukaryotes range from osmoregulation and virulence in parasitic protozoa to modulating blood coagulation, inflammation, bone mineralization and …