Explanipedia

Molecular Cloning of Two New Human Paralogs of 85-kDa Cytosolic Phospholipase A2 Open

Richard T. Pickard, Beth A. Strifler, Ruth M. Kramer, John Sharp · 1999

Two new cloned human cDNAs encode paralogs of the 85-kDa cytosolic phospholipase A2 (cPLA2). We propose to call these cPLA2beta (114 kDa) and cPLA2gamma (61 kDa), giving the name cPLA2alpha to the well known 85-kDa enzyme. cPLA2beta mRNA i…

Identification of the Phosphorylation Sites of Cytosolic Phospholipase A2 in Agonist-stimulated Human Platelets and HeLa Cells Open

Angelika Börsch-Haubold, Fulvia Bártoli, Judith Asselin, Thomas Dudler, Ruth M. Kramer , et al. · 1998

The present study identifies the phosphorylation sites of the 85-kDa cytosolic phospholipase A2 (cPLA2) in human platelets and HeLa cells. Tryptic digests of 32P-phosphorylated and -immunoprecipitated cPLA2 were analyzed by microbore high …

Human group II 14 kDa phospholipase A2 activates human platelets Open

János Polgár, Ruth M. Kramer, Suzane L. Um, Joseph A. Jakubowski, Kenneth J. Clemetson · 1997

Recombinant human group II phospholipase A2 (sPLA2) added to human platelets in the low μg/ml range induced platelet activation, as demonstrated by measurement of platelet aggregation, thromboxane A2 generation and influx of intracellular …

p38 Mitogen-activated Protein Kinase Phosphorylates Cytosolic Phospholipase A2 (cPLA2) in Thrombin-stimulated Platelets Open

Ruth M. Kramer, Edda F. Roberts, Suzane L. Um, Angelika Börsch-Haubold, Steve P. Watson , et al. · 1996

The Ca2+-sensitive 85-kDa cytosolic phospholipase A2 (cPLA2) is responsible for thrombin-stimulated mobilization of arachidonic acid for the synthesis of thromboxane A2 in human platelets. We have previously shown that thrombin activates p…

Inhibition of mitogen-activated protein kinase kinase does not impair primary activation of human platelets Open

Angelika Börsch-Haubold, Ruth M. Kramer, Steve P. Watson · 1996

Mitogen-activated protein kinases (MAPKs), a family of protein serine/threonine kinases regulating cell growth and differentiation, are activated by a dual-specificity kinase through phosphorylation at threonine and tyrosine. We used a rec…

Identification of Essential Residues for the Catalytic Function of 85-kDa Cytosolic Phospholipase A2 Open

Richard T. Pickard, X. Grace Chiou, Beth A. Strifler, Michael R. DeFelippis, Paul A. Hyslop , et al. · 1996

Cytosolic phospholipase A2 (cPLA2) hydrolyzes the sn-2-acyl ester bond of phospholipids and shows a preference for arachidonic acid-containing substrates. We found previously that Ser-228 is essential for enzyme activity and is likely to f…

Author Index Open

Salvatore Sechi, Peter P. Roller, Jami Willette‐Brown, Jean Pierre Kinet, Roger Gilchrist , et al. · 1996

Identification of the ␣ chain lysine donor sites involved in factor XIII a fibrin cross-linking

Thrombin Induces Activation of p38 MAP Kinase in Human Platelets Open

Ruth M. Kramer, Edda F. Roberts, Beth A. Strifler, Edward M. Johnstone · 1995

In human platelets a proline-directed kinase distinct from the ERK MAP kinases is stimulated by both thrombin and the thrombin receptor agonist peptide SFLLRN and may be involved in the activation of Ca(2+)-dependent cytosolic phospholipas…

Cytosolic Phospholipase A2 Is Phosphorylated in Collagen- and Thrombin-stimulated Human Platelets Independent of Protein Kinase C and Mitogen-activated Protein Kinase Open

Angelika Börsch-Haubold, Ruth M. Kramer, Steve P. Watson · 1995

Human platelets pretreated with indomethacin release arachidonic acid predominantly through the activity of cytosolic phospholipase A2 (cPLA2), an 85-kDa protein. This enzyme is regulated by an increase in intracellular Ca2+, a necessary c…

Identification of the mechanism for the inhibition of Na+,K(+)-adenosine triphosphatase by hyperglycemia involving activation of protein kinase C and cytosolic phospholipase A2. Open

Pu Xia, Ruth M. Kramer, G L King · 1995

Inhibition of Na+,K(+)-ATPase activity by hyperglycemia could be an important etiological factor of chronic complications in diabetic patients. The biochemical mechanism underlying hyperglycemia's inhibitory effects has been thought to inv…

Differential Activation of Cytosolic Phospholipase A2 (cPLA2) by Thrombin and Thrombin Receptor Agonist Peptide in Human Platelets. EVIDENCE FOR ACTIVATION OF cPLA2 INDEPENDENT OF THE MITOGEN-ACTIVATED PROTEIN KINASES ERK1/2 Open

Ruth M. Kramer, Edda F. Roberts, Paul A. Hyslop, Barbara G. Utterback, Kwan Y. Hui , et al. · 1995

The thrombin receptor agonist peptide SFLLRN was less effective than thrombin in eliciting the liberation of arachidonic acid and the generation of thromboxane A2 by human platelets. We found that while SFLLRN evokes an initial transient i…

Phosphorylation and activation of Ca2+-sensitive cytosolic phospholipase A2 in MCII mast cells mediated by high-affinity Fc receptor for IgE Open

Susan Currie, E F Roberts, Stephen M. Spaethe, Neal W. Roehm, Ruth M. Kramer · 1994

In the present study we examined the activation of Ca(2+)-sensitive cytosolic phospholipase A2 (cPLA2) after aggregation of cell-surface high-affinity Fc receptors for IgE (Fc epsilon RI) on mast cells. MCII mast cells (a factor-dependent …

Pancreatic-type phospholipase A2 induces group II phospholipase A2 expression and prostaglandin biosynthesis in rat mesangial cells. Open

Junji Kishino, Osamu Ohara, Koji Nomura, Ruth M. Kramer, H. Arita · 1994

The effect of pancreatic group I phospholipase A2 (PLA2-I) on receptor-mediated expression of arthritic group II phospholipase A2 (PLA2-II) and its correlation with prostaglandin E2 (PGE2) synthesis were examined in cultured rat mesangial …

Thrombin-induced phosphorylation and activation of Ca(2+)-sensitive cytosolic phospholipase A2 in human platelets. Open

Ruth M. Kramer, E F Roberts, Joseph Manetta, Paul A. Hyslop, Joseph A. Jakubowski · 1993

Receptor-mediated activation of human platelets by thrombin initiates a series of rapid biochemical events that include activation of phospholipase A2 to liberate arachidonic acid for further conversion to thromboxane A2. The identity of t…

The Ca2(+)-sensitive cytosolic phospholipase A2 is a 100-kDa protein in human monoblast U937 cells. Open

Ruth M. Kramer, E F Roberts, Joseph Manetta, J. E Putnam · 1991

Human monoblast U937 cells contain a soluble phospholipase A2 (PLA2) that is activated over the range of 150-600 nM Ca2+ and is stable only at neutral pH. We have purified this PLA2 over 34,000-fold to near homogeneity using sequential ion…

Structure and Properties of a Human Non-pancreatic Phospholipase A2 Open

Ruth M. Kramer, Catherine Hession, Berit Johansen, Gabriela A. Hayes, Paula McGray , et al. · 1989

We have purified a human non-pancreatic phospholipase A2 that is present in platelets and is enriched in rheumatoid synovial fluid. The enzyme is calcium-dependent, has a pH optimum of 8-10, and shows a striking preference for substrate pr…

Stimulation of Ca2+-activated human platelet phospholipase A2 by diacylglycerol Open

Ruth M. Kramer, Gregg Checani, Daniel Deykin · 1987

We examined the effect of diacylglycerol on Ca2+-dependent phospholipase A2 from human platelets. Phospholipase A2 was solubilized and partially purified to a stable form in the presence of n-octyl beta-D-glucopyranoside (octyl glucoside),…

Metabolism of platelet-activating factor in human platelets. Transacylase-mediated synthesis of 1-O-alkyl-2-arachidonoyl-sn-glycero-3-phosphocholine. Open

Ruth M. Kramer, George M. Patton, Carole R. Pritzker, Daniel Deykin · 1984

The present study demonstrates that inactivation of exogenous 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (alkylacetyl-GPC; platelet-activating factor) by human platelets is mediated by the sequential action of two enzymes, 1) a Ca2+-in…

Coenzyme A-mediated arachidonic acid transacylation in human platelets. Open

Ruth M. Kramer, Carole R. Pritzker, Daniel Deykin · 1984

Platelet membranes contain two distinct transacylase activities catalyzing the synthesis of arachidonoyl phosphatides by acylation of added lysophosphatides with endogenous esterified arachidonate. In the absence of CoA, arachidonate is in…

Arachidonoyl transacylase in human platelets. Coenzyme A-independent transfer of arachidonate from phosphatidylcholine to lysoplasmenylethanolamine. Open

Ruth M. Kramer, Daniel Deykin · 1983

Human platelets contain an enzyme that catalyzes CoA-independent release of arachidonic acid from phosphatidylcholine with concomitant incorporation into plasmenylethanolamine. Addition of lysoplasmenylethanolamine (10-80 microM) to a crud…

Effect of membrane cholesterol on phospholipid metabolism in thrombin-stimulated platelets. Enhanced activation of platelet phospholipase(s) for liberation of arachidonic acid. Open

Ruth M. Kramer, J.A. Jakubowski, R. Vaillancourt, Daniel Deykin · 1982

The cholesterol to phospholipid mole ratio (C/PL) of human platelets was increased 1.3-fold or maintained at a normal value by incubating platelets with sonicated dispersions of cholesterol and phosphatidylcholine (PC) (C/PL = 3 or 1, resp…

On the Mechanism of Na<sup>+</sup>‐and K<sup>+</sup>‐Stimulated Hydrolysis of Adenosine Triphosphate Open

W. Schöner, C. von Ilberg, Ruth M. Kramer, W. Seubert · 1967

The purification and properties of a Na + ‐ and K + ‐activated ATPase from ox brain is described. The enzyme preparation is characterized by a high purity and a low content (<1%) of Mg ++ ‐stimulated ATPase.

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