Ruth E. Silversmith
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View article: Alteration of a Nonconserved Active Site Residue in the Chemotaxis Response Regulator CheY Affects Phosphorylation and Interaction with CheZ
Alteration of a Nonconserved Active Site Residue in the Chemotaxis Response Regulator CheY Affects Phosphorylation and Interaction with CheZ Open
CheY is a response regulator in the well studied two-component system that mediates bacterial chemotaxis. Phosphorylation of CheY at Asp(57) enhances its interaction with the flagellar motor. Asn(59) is located near the phosphorylation sit…
View article: Kinetic Characterization of Catalysis by the Chemotaxis Phosphatase CheZ: MODULATION OF ACTIVITY BY THE PHOSPHORYLATED CheY SUBSTRATE
Kinetic Characterization of Catalysis by the Chemotaxis Phosphatase CheZ: MODULATION OF ACTIVITY BY THE PHOSPHORYLATED CheY SUBSTRATE Open
CheZ catalyzes the dephosphorylation of the response regulator CheY in the two-component regulatory system that mediates chemotaxis in Escherichia coli. CheZ is a homodimer with two active sites for dephosphorylation. To gain insight into …
View article: Role of Position K+4 in the Phosphorylation and Dephosphorylation Reaction Kinetics of the CheY Response Regulator
Role of Position K+4 in the Phosphorylation and Dephosphorylation Reaction Kinetics of the CheY Response Regulator Open
Two-component signaling is a primary method by which microorganisms interact with their environments. A kinase detects stimuli and modulates autophosphorylation activity. The signal propagates by phosphotransfer from the kinase to a respon…
View article: A Link between Dimerization and Autophosphorylation of the Response Regulator PhoB
A Link between Dimerization and Autophosphorylation of the Response Regulator PhoB Open
Response regulator proteins within two-component signal transduction systems are activated by phosphorylation and can catalyze their own covalent phosphorylation using small molecule phosphodonors. To date, comprehensive kinetic characteri…
View article: Identical phosphatase mechanisms achieved through distinct modes of binding phosphoprotein substrate
Identical phosphatase mechanisms achieved through distinct modes of binding phosphoprotein substrate Open
Two-component signal transduction systems are widespread in prokaryotes and control numerous cellular processes. Extensive investigation of sensor kinase and response regulator proteins from many two-component systems has established conse…
View article: Conformational coupling in the chemotaxis response regulator CheY
Conformational coupling in the chemotaxis response regulator CheY Open
CheY, a response regulator protein in bacterial chemotaxis, serves as a prototype for the analysis of response regulator function in two-component signal transduction. Phosphorylation of a conserved aspartate at the active site mediates a …
View article: CheZ-Mediated Dephosphorylation of the Escherichia coli Chemotaxis Response Regulator CheY: Role for CheY Glutamate 89
CheZ-Mediated Dephosphorylation of the Escherichia coli Chemotaxis Response Regulator CheY: Role for CheY Glutamate 89 Open
The swimming behavior of Escherichia coli at any moment is dictated by the intracellular concentration of the phosphorylated form of the chemotaxis response regulator CheY, which binds to the base of the flagellar motor. CheY is phosphoryl…
View article: Matching Biochemical Reaction Kinetics to the Timescales of Life: Structural Determinants That Influence the Autodephosphorylation Rate of Response Regulator Proteins
Matching Biochemical Reaction Kinetics to the Timescales of Life: Structural Determinants That Influence the Autodephosphorylation Rate of Response Regulator Proteins Open
In two-component regulatory systems, covalent phosphorylation typically activates the response regulator signaling protein and hydrolysis of the phosphoryl group reestablishes the inactive state. Despite highly conserved three-dimensional …
View article: Isolation and Characterization of Nonchemotactic CheZ Mutants of Escherichia coli
Isolation and Characterization of Nonchemotactic CheZ Mutants of Escherichia coli Open
The Escherichia coli CheZ protein stimulates dephosphorylation of CheY, a response regulator in the chemotaxis signal transduction pathway, by an unknown mechanism. Genetic analysis of CheZ has lagged behind biochemical and biophysical cha…
View article: CheX Is a Phosphorylated CheY Phosphatase Essential for Borrelia burgdorferi Chemotaxis
CheX Is a Phosphorylated CheY Phosphatase Essential for Borrelia burgdorferi Chemotaxis Open
Motility and chemotaxis are believed to be important in the pathogenesis of Lyme disease caused by the spirochete Borrelia burgdorferi. Controlling the phosphorylation state of CheY, a response regulator protein, is essential for regulatin…
View article: Action at a Distance: Amino Acid Substitutions That Affect Binding of the Phosphorylated CheY Response Regulator and Catalysis of Dephosphorylation Can Be Far from the CheZ Phosphatase Active Site
Action at a Distance: Amino Acid Substitutions That Affect Binding of the Phosphorylated CheY Response Regulator and Catalysis of Dephosphorylation Can Be Far from the CheZ Phosphatase Active Site Open
Two-component regulatory systems, in which phosphorylation controls the activity of a response regulator protein, provide signal transduction in bacteria. For example, the phosphorylated CheY response regulator (CheYp) controls swimming be…
View article: Investigation of the Role of Electrostatic Charge in Activation of the Escherichia coli Response Regulator CheY
Investigation of the Role of Electrostatic Charge in Activation of the Escherichia coli Response Regulator CheY Open
In a two-component regulatory system, an important means of signal transduction in microorganisms, a sensor kinase phosphorylates a response regulator protein on an aspartyl residue, resulting in activation. The active site of the response…
View article: Auxiliary phosphatases in two-component signal transduction
Auxiliary phosphatases in two-component signal transduction Open
Signal termination in two-component systems occurs by loss of the phosphoryl group from the response regulator protein. This review explores our current understanding of the structures, catalytic mechanisms and means of regulation of the k…
View article: Nonconserved Active Site Residues Modulate CheY Autophosphorylation Kinetics and Phosphodonor Preference
Nonconserved Active Site Residues Modulate CheY Autophosphorylation Kinetics and Phosphodonor Preference Open
In two-component signal transduction, response regulator proteins contain the catalytic machinery for their own covalent phosphorylation and can catalyze phosphotransfer from a partner sensor kinase or autophosphorylate using various small…
View article: Imidazole as a Small Molecule Analogue in Two-Component Signal Transduction
Imidazole as a Small Molecule Analogue in Two-Component Signal Transduction Open
In two-component signal transduction systems (TCSs), responses to stimuli are mediated through phosphotransfer between protein components. Canonical TCSs use His → Asp phosphotransfer in which phosphoryl groups are transferred from a conse…
View article: Probing Mechanistic Similarities between Response Regulator Signaling Proteins and Haloacid Dehalogenase Phosphatases
Probing Mechanistic Similarities between Response Regulator Signaling Proteins and Haloacid Dehalogenase Phosphatases Open
Response regulator signaling proteins and phosphatases of the haloacid dehalogenase (HAD) superfamily share strikingly similar folds, active site geometries, and reaction chemistry. Proteins from both families catalyze the transfer of a ph…
View article: Phosphoryl Group Flow within the Pseudomonas aeruginosa Pil-Chp Chemosensory System: DIFFERENTIAL FUNCTION OF THE EIGHT PHOSPHOTRANSFERASE AND THREE RECEIVER DOMAINS
Phosphoryl Group Flow within the Pseudomonas aeruginosa Pil-Chp Chemosensory System: DIFFERENTIAL FUNCTION OF THE EIGHT PHOSPHOTRANSFERASE AND THREE RECEIVER DOMAINS Open
Bacterial chemosensory signal transduction systems that regulate motility by type IV pili (T4P) can be markedly more complex than related flagellum-based chemotaxis systems. In T4P-based systems, the CheA kinase often contains numerous pot…
View article: A Variable Active Site Residue Influences the Kinetics of Response Regulator Phosphorylation and Dephosphorylation
A Variable Active Site Residue Influences the Kinetics of Response Regulator Phosphorylation and Dephosphorylation Open
Two-component regulatory systems, minimally composed of a sensor kinase and a response regulator protein, are common mediators of signal transduction in microorganisms. All response regulators contain a receiver domain with conserved activ…
View article: A Variable Active Site Residue Influences the Kinetics of Response Regulator Phosphorylation and Dephosphorylation
A Variable Active Site Residue Influences the Kinetics of Response Regulator Phosphorylation and Dephosphorylation Open
Two-component regulatory systems, minimally composed of a sensor kinase and a response regulator protein, are common mediators of signal transduction in microorganisms. All response regulators contain a receiver domain with conserved activ…
View article: Phosphoryl Group Flow within the Pseudomonas aeruginosa Pil-Chp Chemosensory System
Phosphoryl Group Flow within the Pseudomonas aeruginosa Pil-Chp Chemosensory System Open
Bacterial chemosensory signal transduction systems that regulate motility by type IV pili (T4P) can be markedly more complex than related flagellum-based chemotaxis systems. In T4P-based systems, the CheA kinase often contains numerous pot…