Ruth Brack‐Werner
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View article: Molecular Signature of Astrocytes for Gene Delivery by the Synthetic Adeno‐Associated Viral Vector rAAV9P1
Molecular Signature of Astrocytes for Gene Delivery by the Synthetic Adeno‐Associated Viral Vector rAAV9P1 Open
Astrocytes have crucial functions in the central nervous system (CNS) and are major players in many CNS diseases. Research on astrocyte‐centered diseases requires efficient and well‐characterized gene transfer vectors. Vectors derived from…
View article: ATO (Arsenic Trioxide) Effects on Promyelocytic Leukemia Nuclear Bodies Reveals Antiviral Intervention Capacity
ATO (Arsenic Trioxide) Effects on Promyelocytic Leukemia Nuclear Bodies Reveals Antiviral Intervention Capacity Open
Human adenoviruses (HAdV) are associated with clinical symptoms such as gastroenteritis, keratoconjunctivitis, pneumonia, hepatitis, and encephalitis. In the absence of protective immunity, as in allogeneic bone marrow transplant patients,…
View article: SKP2 attenuates autophagy through Beclin1-ubiquitination and its inhibition reduces MERS-Coronavirus infection
SKP2 attenuates autophagy through Beclin1-ubiquitination and its inhibition reduces MERS-Coronavirus infection Open
Autophagy is an essential cellular process affecting virus infections and other diseases and Beclin1 (BECN1) is one of its key regulators. Here, we identified S-phase kinase-associated protein 2 (SKP2) as E3 ligase that executes lysine-48-…
View article: Chemoenzymatic Total Synthesis of Sorbicatechol Structural Analogues and Evaluation of Their Antiviral Potential
Chemoenzymatic Total Synthesis of Sorbicatechol Structural Analogues and Evaluation of Their Antiviral Potential Open
Sorbicillinoids are fungal polyketides characterized by highly complex and diverse molecular structures, with considerable stereochemical intricacy combined with a high degree of oxygenation. Many sorbicillinoids possess promising biologic…
View article: Modulation of HIV-1 gene expression by binding of a ULM motif in the Rev protein to UHM-containing splicing factors
Modulation of HIV-1 gene expression by binding of a ULM motif in the Rev protein to UHM-containing splicing factors Open
The HIV-1 protein Rev is essential for virus replication and ensures the expression of partially spliced and unspliced transcripts. We identified a ULM (UHM ligand motif) motif in the Arginine-Rich Motif (ARM) of the Rev protein. ULMs (UHM…
View article: Biological Evaluation of Molecules of the azaBINOL Class as Antiviral Agents: Specific Inhibition of HIV-1 RNase H Activity by 7-Isopropoxy-8-(naphth-1-yl)quinoline
Biological Evaluation of Molecules of the azaBINOL Class as Antiviral Agents: Specific Inhibition of HIV-1 RNase H Activity by 7-Isopropoxy-8-(naphth-1-yl)quinoline Open
Inspired by bioactive biaryl-containing natural products found in plants and the marine environment, a series of synthetic compounds belonging to the azaBINOL chiral ligand family was evaluated for antiviral activity against HIV-1. Testing…
View article: Dual role of the chromatin-binding factor PHF13 in the pre- and post-integration phases of HIV-1 replication
Dual role of the chromatin-binding factor PHF13 in the pre- and post-integration phases of HIV-1 replication Open
Viruses interact with multiple host cell factors. Some of these are required to promote viral propagation, others have roles in inhibiting infection. Here, we delineate the function of the cellular factor PHF13 (or SPOC1), a putative HIV-1…
View article: Supplementary material from "Dual role of the chromatin-binding factor PHF13 in the pre- and post-integration phases of HIV-1 replication"
Supplementary material from "Dual role of the chromatin-binding factor PHF13 in the pre- and post-integration phases of HIV-1 replication" Open
Viruses interact with multiple host cell factors. Some of these are required to promote viral propagation, others have roles in inhibiting infection. Here, we delineate the function of the cellular factor PHF13 (or SPOC1), a putative HIV-1…
View article: Figure S4: Analysis of knockdown efficiency in HIV-1-infected U2OS-C5 cells from Dual role of the chromatin-binding factor PHF13 in the pre- and post-integration phases of HIV-1 replication
Figure S4: Analysis of knockdown efficiency in HIV-1-infected U2OS-C5 cells from Dual role of the chromatin-binding factor PHF13 in the pre- and post-integration phases of HIV-1 replication Open
U2OS-C5 cells were mock transfected or with a control siRNA (csiRNA) or directed against PHF13 (siPHF13). 24 h later cells were infected with 100 ng p24 VSVG pseudotyped HIV-1 NL4-3 IRES-eGFP or the ΔVpr variant for 6 h, washed and incubat…
View article: Figure S1: HIV-1 reduces PHF13 expression in SupT1 and Jurkat-TAg cells from Dual role of the chromatin-binding factor PHF13 in the pre- and post-integration phases of HIV-1 replication
Figure S1: HIV-1 reduces PHF13 expression in SupT1 and Jurkat-TAg cells from Dual role of the chromatin-binding factor PHF13 in the pre- and post-integration phases of HIV-1 replication Open
Jurkat-TAg or SupT1 cells were infected with 200 ng p24 VSVG pseudotyped HIV-1 NL4-3 or mock infected. Cells were harvested 48 hpi, total cell extracts were prepared and analyzed for expression of PHF13, HIV-1 capsid p24 and tubulin by imm…
View article: Figure S2: No effects of doxycycline treatment on HIV-1 infection in parental U2OS cells. from Dual role of the chromatin-binding factor PHF13 in the pre- and post-integration phases of HIV-1 replication
Figure S2: No effects of doxycycline treatment on HIV-1 infection in parental U2OS cells. from Dual role of the chromatin-binding factor PHF13 in the pre- and post-integration phases of HIV-1 replication Open
U2OS cells were treated with 1 μg/ml doxycycline or left untreated for 24 h before cells were infected with 100 ng p24 VSVG pseudotyped HIV-1 NL4-3 IRESeGFP. 24 hpi cells were analyzed by flow cytometry. The mean percentage of GFP+ cells f…
View article: T cells with low CD2 levels express reduced restriction factors and are preferentially infected in therapy naïve chronic HIV‐1 patients
T cells with low CD2 levels express reduced restriction factors and are preferentially infected in therapy naïve chronic HIV‐1 patients Open
Introduction : Restriction factors (RFs) suppress HIV‐1 in cell lines and primary cell models. Hence, RFs might be attractive targets for novel antiviral strategies, but their importance for virus control in vivo is controversial. Methods …
View article: Figure S5: Effect of Interferon-α on PHF13 expression from Dual role of the chromatin-binding factor PHF13 in the pre- and post-integration phases of HIV-1 replication
Figure S5: Effect of Interferon-α on PHF13 expression from Dual role of the chromatin-binding factor PHF13 in the pre- and post-integration phases of HIV-1 replication Open
293T cells were treated with the indicated amounts of recombinant Interferon-α and cultured for 6 h or 24 h. At the time points indicated cells were lysed to detect PHF13, tetherin and actin by immunoblot.
View article: Figure S3: No effects on HIV-1 LTRtransactivation when parental U2OS cells were treated with doxycycline post infection from Dual role of the chromatin-binding factor PHF13 in the pre- and post-integration phases of HIV-1 replication
Figure S3: No effects on HIV-1 LTRtransactivation when parental U2OS cells were treated with doxycycline post infection from Dual role of the chromatin-binding factor PHF13 in the pre- and post-integration phases of HIV-1 replication Open
U2OS cells were infected with 100 ng p24 VSVG pseudotyped HIV-1 NL4-3 IRES-eGFP for 24 h. Thereafter, cells were treated with 1 μg/ml doxycycline.Additional 24 h later cells were analyzed by flow cytometry for the % and MFI of GFP+ (HIV-1-…
View article: Supramolecular combinations of humic polyanions as potent microbicides with polymodal anti-HIV-activities
Supramolecular combinations of humic polyanions as potent microbicides with polymodal anti-HIV-activities Open
Anti-HIV potency of humic PAs is governed by scaffolds diversity.
View article: Alkaloids from the Sponge Stylissa carteri Present Prospective Scaffolds for the Inhibition of Human Immunodeficiency Virus 1 (HIV-1)
Alkaloids from the Sponge Stylissa carteri Present Prospective Scaffolds for the Inhibition of Human Immunodeficiency Virus 1 (HIV-1) Open
The sponge Stylissa carteri is known to produce a number of secondary metabolites displaying anti-fouling, anti-inflammatory, and anti-cancer activity. However, the anti-viral potential of metabolites produced by S. carteri has not been ex…