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View article: The Lysis Cassette of Jumbophage phiKZ
The Lysis Cassette of Jumbophage phiKZ Open
Pseudomonas jumbophage phiKZ is prominent because of the proteinaceous nuclear structure that is formed during the infection cycle, conferring resistance of the replicating phage DNA to anti-phage resistance systems. Progress has been made…
View article: Resolution of a T1-Like Bacteriophage Outbreak by Receptor Engineering
Resolution of a T1-Like Bacteriophage Outbreak by Receptor Engineering Open
Bacteriophage contaminations pose substantial risks to biomolecular production pipelines, and their resolution is especially difficult when the identity of the offending agent is unknown. We recently experienced an outbreak of Escherichia …
View article: Structural basis of Acinetobacter type IV pili targeting by an RNA virus
Structural basis of Acinetobacter type IV pili targeting by an RNA virus Open
Acinetobacters pose a significant threat to human health, especially those with weakened immune systems. Type IV pili of acinetobacters play crucial roles in virulence and antibiotic resistance. Single-stranded RNA bacteriophages target th…
View article: Physiological characterization of single-gene lysis proteins
Physiological characterization of single-gene lysis proteins Open
Single-strand RNA (ssRNA) and single-strand DNA phages elicit host lysis using a single gene, in each case designated as sgl . Of the 11 identified Sgls, three have been shown to be specific inhibitors of different steps in the pathway tha…
View article: Spatial and temporal control of lysis by the lambda holin
Spatial and temporal control of lysis by the lambda holin Open
In this study, we use fluorescent video microscopy to track -green fluorescent protein (GFP)-labeled holin in the minutes prior to phage lysis. Our work contextualizes prior genetic and biochemical data, showing when hole formation starts …
View article: Complete genomic analysis of <i>Escherichia</i> phage Mangalyan infecting <i>Escherichia fergusonii</i>
Complete genomic analysis of <i>Escherichia</i> phage Mangalyan infecting <i>Escherichia fergusonii</i> Open
Escherichia fergusonii is a rarely isolated opportunistic pathogen in animals and humans. Here, we present the annotated genome sequence of Escherichia phage Mangalyan, a T4-like bacteriophage infecting E. fergusonii isolated from chickens…
View article: Systematic and scalable genome-wide essentiality mapping to identify nonessential genes in phages
Systematic and scalable genome-wide essentiality mapping to identify nonessential genes in phages Open
Phages are one of the key ecological drivers of microbial community dynamics, function, and evolution. Despite their importance in bacterial ecology and evolutionary processes, phage genes are poorly characterized, hampering their usage in…
View article: Physiological characterization of single gene lysis proteins
Physiological characterization of single gene lysis proteins Open
Until recently only 11 distinct Sgls ( si ngle g ene lysis proteins) have been experimentally identified. Of these, three have been shown to be specific inhibitors of different steps in the pathway that supplies Lipid II to the peptidoglyc…
View article: Spatial and temporal control of lysis by the lambda holin
Spatial and temporal control of lysis by the lambda holin Open
The infection cycle of phage λ terminates in lysis mediated by three types of lysis proteins, each disrupting a layer in the bacterial envelope: the S105 holin, the R endolysin, and the Rz/Rz1 spanin complex targeting the inner membrane (I…
View article: Genome-wide CRISPRi knockdown to map gene essentiality landscape in coliphages λ and P1
Genome-wide CRISPRi knockdown to map gene essentiality landscape in coliphages λ and P1 Open
Phages are one of the key ecological drivers of microbial community dynamics, function and evolution. Despite their importance in bacterial ecology and evolutionary processes, phage genes are poorly characterized, hampering their usage in …
View article: Hybrid Vigor: Importance of Hybrid λ Phages in Early Insights in Molecular Biology
Hybrid Vigor: Importance of Hybrid λ Phages in Early Insights in Molecular Biology Open
Laboratory-generated hybrids between phage λ and related phages played a seminal role in establishment of the λ model system, which, in turn, served to develop many of the foundational concepts of molecular biology, including gene structur…
View article: The transcriptional regulator CtrA controls gene expression in Alphaproteobacteria phages: Evidence for a lytic deferment pathway
The transcriptional regulator CtrA controls gene expression in Alphaproteobacteria phages: Evidence for a lytic deferment pathway Open
Pilitropic and flagellotropic phages adsorb to bacterial pili and flagella. These phages have long been used to investigate multiple aspects of bacterial physiology, such as the cell cycle control in the Caulobacterales. Targeting cellular…
View article: Comparative genomics of Acinetobacter baumannii and therapeutic bacteriophages from a patient undergoing phage therapy
Comparative genomics of Acinetobacter baumannii and therapeutic bacteriophages from a patient undergoing phage therapy Open
In 2016, a 68-year-old patient with a disseminated multidrug-resistant Acinetobacter baumannii infection was successfully treated using lytic bacteriophages. Here we report the genomes of the nine phages used for treatment and three strain…
View article: Endolysin Regulation in Phage Mu Lysis
Endolysin Regulation in Phage Mu Lysis Open
Host cell lysis is the terminal event of the bacteriophage infection cycle. In Gram-negative hosts, lysis requires proteins that disrupt each of the three cell envelope components, only one of which has been identified in Mu: the endolysin…
View article: Complete Genome Sequences of Lambdoid Phages 21, 434, and 434B and Several Lambda Hybrids
Complete Genome Sequences of Lambdoid Phages 21, 434, and 434B and Several Lambda Hybrids Open
Recombinational hybrids between phage λ and its relatives were instrumental in the beginnings of molecular biology. Here, we report the complete genome sequences of lambdoid phages 21 and 434 and three of their λ hybrids. In addition, we d…
View article: Complete Genome Sequence of Enterococcus faecalis Siphophage Sigurd
Complete Genome Sequence of Enterococcus faecalis Siphophage Sigurd Open
Enterococcus faecalis is associated with antibiotic-resistant infections, and this study presents E. faecalis siphophage Sigurd. The 41,811-bp Sigurd genome is divided into two arms defined by long convergent predicted transcription units …
View article: Complete Genome Sequence of Alcaligenes faecalis Phage Piluca
Complete Genome Sequence of Alcaligenes faecalis Phage Piluca Open
Alcaligenes faecalis is an opportunistic pathogen exhibiting drug resistance. Here, the 35,451-bp genome of A. faecalis phage Piluca is described. Piluca is not closely related to any isolated phages in the NCBI database. Piluca possesses …
View article: Complete Genome Sequence of Stenotrophomonas maltophilia Siphophage Silvanus
Complete Genome Sequence of Stenotrophomonas maltophilia Siphophage Silvanus Open
Stenotrophomonas maltophilia is an opportunistic Gram-negative bacterium capable of causing respiratory infections. S. maltophilia siphophage Silvanus was isolated, and its 45,678-bp genome is not closely related to known phages based on w…
View article: Complete Genome Sequence of Stenotrophomonas maltophilia Myophage Marzo
Complete Genome Sequence of Stenotrophomonas maltophilia Myophage Marzo Open
Stenotrophomonas maltophilia is a Gram-negative opportunistic bacterium that is increasingly being associated with infections. Here, we report the complete genome of the S. maltophilia myophage Marzo, with a 159,384-bp genome encoding 268 …
View article: Parallel multicopy-suppressor screens reveal convergent evolution of phage-encoded single gene lysis proteins
Parallel multicopy-suppressor screens reveal convergent evolution of phage-encoded single gene lysis proteins Open
In contrast to dsDNA phages where multiple proteins are involved in programmed host lysis, lysis in ssRNA Fiersviridae and ssDNA Microviridae phages requires only a single gene ( sgl for s ingle g ene l ysis ) to meet the size constraints …
View article: Comparative genomics of <i>Acinetobacter baumannii</i> and therapeutic bacteriophages from a patient undergoing phage therapy
Comparative genomics of <i>Acinetobacter baumannii</i> and therapeutic bacteriophages from a patient undergoing phage therapy Open
In 2016, a 68-year-old patient with a disseminated multi-drug resistant Acinetobacter baumannii infection was treated using lytic bacteriophages in one of the first modern human clinical uses of phage therapy in the United States. Due to t…
View article: The Phage T4 Antiholin RI Has a Cleavable Signal Peptide, Not a SAR Domain
The Phage T4 Antiholin RI Has a Cleavable Signal Peptide, Not a SAR Domain Open
Holin/endolysin-mediated lysis of phage T4 of Escherichia coli is tightly regulated by the antiholins RI and RIII. While regulation by the cytoplasmic RIII plays a minor role, the periplasmic antiholin RI binds tightly to the holin T and i…
View article: Phage-Encoded Cationic Antimicrobial Peptide Required for Lysis
Phage-Encoded Cationic Antimicrobial Peptide Required for Lysis Open
We provide evidence that phiKT produces an antimicrobial peptide for outer membrane disruption during lysis. This protein, designated a disruptin, is a new paradigm for phage lysis and has no similarities to other known lysis genes.
View article: Complete Genome Sequence of Bradyrhizobium japonicum Podophage Paso
Complete Genome Sequence of Bradyrhizobium japonicum Podophage Paso Open
Bradyrhizobium japonicum is a nitrogen-fixing, Gram-negative bacterium that forms a symbiotic relationship with leguminous plants. This announcement describes the isolation and genome annotation of B. japonicum T7-like podophage Paso. Geno…
View article: Complete Genome Sequence of Burkholderia gladioli Phage Maja
Complete Genome Sequence of Burkholderia gladioli Phage Maja Open
Burkholderia gladioli is a Gram-negative bacterium associated with cystic fibrosis infections. Here, we describe the genome sequence of B. gladioli phage Maja. Maja is most related to another Burkholderia phage, BcepF1, and may be a temper…
View article: Complete Genome Sequence of Rhizobium phaseoli Podophage Palo
Complete Genome Sequence of Rhizobium phaseoli Podophage Palo Open
Here, we present the genome of Palo, a T7-like podophage of Rhizobium phaseoli . The genome is 46.3 kb and contains 58 predicted protein-coding genes, including a novel signal-anchor-release (SAR) endolysin, a homolog of the T5 A1 protein …