Ryan Beckner
YOU?
Author Swipe
View article: Chiral inversion mutagenesis identifies geometrically constrained residues within self-associating low-complexity domains
Chiral inversion mutagenesis identifies geometrically constrained residues within self-associating low-complexity domains Open
Many protein low-complexity domains (LCDs) reversibly self-associate to enable cellular function, yet fundamental questions remain regarding how polypeptide chemical and structural features beyond side chain identity contribute to LCD-LCD …
View article: A germline <i>ETV6</i> mutation disrupts hematopoiesis via de novo creation of a nuclear export signal
A germline <i>ETV6</i> mutation disrupts hematopoiesis via de novo creation of a nuclear export signal Open
Germline mutations in the transcriptional regulator ETV6 are a root cause of familial inherited thrombocytopenia and predispose carriers to myelodysplastic syndromes and acute leukemias. Here, we report that the ETV6 P214L mutation creates…
View article: Enigmatic missense mutations can cause disease via creation of<i>de novo</i>nuclear export signals
Enigmatic missense mutations can cause disease via creation of<i>de novo</i>nuclear export signals Open
Summary Disease-causing missense mutations that occur within structurally and functionally unannotated protein regions can guide researchers to new mechanisms of protein regulation and dysfunction. Here, we report that the thrombocytopenia…
View article: Abstract 2310 Unexpected mechanisms of ETV6 dysfunction in hematologic disease
Abstract 2310 Unexpected mechanisms of ETV6 dysfunction in hematologic disease Open
Germline and somatic missense mutations in the transcriptional repressor ETV6 are strongly associated with blood diseases, including thrombocytopenia and leukemia. These dominant negative mutations, which are scattered throughout the ETV6 …
View article: Abstract 2164 Familial Thrombocytopenia-Associated Germline ETV6 P214L Mutation Results in XPO1-Mediated Nuclear Export
Abstract 2164 Familial Thrombocytopenia-Associated Germline ETV6 P214L Mutation Results in XPO1-Mediated Nuclear Export Open
Objective: Determine the mechanism by which the recurrent thrombocytopenia-associated germline ETV6 P214L mutation results in protein mislocalization and disease. Methods: We utilized a variety of in vitro biochemical (fluorescence polariz…
View article: Abstract 2169 Probing hidden structure within intrinsically disordered protein domains with chiral mutagenesis
Abstract 2169 Probing hidden structure within intrinsically disordered protein domains with chiral mutagenesis Open
Certain protein intrinsically disordered regions (IDRs) reversibly form higher-order oligomers to fulfill their cellular functions. As IDRs lack predictable structured elements, determination of sequence features that encode IDR oligomeriz…
View article: Familial Thrombocytopenia-Associated Germline ETV6 P214L Mutation Results in XPO1-Mediated Nuclear Export
Familial Thrombocytopenia-Associated Germline ETV6 P214L Mutation Results in XPO1-Mediated Nuclear Export Open
In recent years, a number of heterozygous germline mutations in ETV6 have been associated with familial thrombocytopenia and predisposition to hematologic malignancies. While most of these missense mutations occur in the DNA-binding-domain…
View article: Serine ADP-ribosylation marks nucleosomes for ALC1-dependent chromatin remodeling
Serine ADP-ribosylation marks nucleosomes for ALC1-dependent chromatin remodeling Open
Serine ADP-ribosylation (ADPr) is a DNA damage-induced post-translational modification catalyzed by the PARP1/2:HPF1 complex. As the list of PARP1/2:HPF1 substrates continues to expand, there is a need for technologies to prepare mono- and…
View article: Crystal Structure and Conformational Dynamics of <i>Pyrococcus furiosus</i> Prolyl Oligopeptidase
Crystal Structure and Conformational Dynamics of <i>Pyrococcus furiosus</i> Prolyl Oligopeptidase Open
Enzymes in the prolyl oligopeptidase family possess unique structures and substrate specificities that are important for their biological activity and for potential biocatalytic applications. The crystal structures of Pyrococcus furiosus (…