Ryan M. Allen
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View article: Stereocontrol in Reductive Aminases through Steric Modification of Residues Around Substrate and Cofactor Binding Pockets
Stereocontrol in Reductive Aminases through Steric Modification of Residues Around Substrate and Cofactor Binding Pockets Open
Enzymatic reductive amination catalysed by imine reductases (IREDs) and reductive aminases (RedAms) provides green, stereoselective, and direct access to 2° and 3° chiral amines. Given existing interests in exploiting RedAms for commercial…
View article: Structural optimization of siRNA conjugates for albumin binding achieves effective MCL1-directed cancer therapy
Structural optimization of siRNA conjugates for albumin binding achieves effective MCL1-directed cancer therapy Open
The high potential of siRNAs to silence oncogenic drivers remains largely untapped due to the challenges of tumor cell delivery. Here, divalent lipid-conjugated siRNAs are optimized for in situ binding to albumin to improve pharmacokinetic…
View article: Structural Optimization of siRNA Conjugates for Albumin Binding Achieves Effective MCL1-Targeted Cancer Therapy
Structural Optimization of siRNA Conjugates for Albumin Binding Achieves Effective MCL1-Targeted Cancer Therapy Open
The high potential for therapeutic application of siRNAs to silence traditionally undruggable oncogenic drivers remains largely untapped due to the challenges of tumor cell delivery. Here, siRNAs were optimized for in situ binding to album…
View article: Physico-chemical principles of HDL-small RNA binding interactions
Physico-chemical principles of HDL-small RNA binding interactions Open
Extracellular small RNAs (sRNA) are abundant in many biofluids, but little is known about their mechanisms of transport and stability in RNase-rich environments. We previously reported that high-density lipoproteins (HDL) of mice were enri…
View article: Profile of Podocyte Translatome During Development of Type 2 and Type 1 Diabetic Nephropathy Using Podocyte-Specific TRAP mRNA RNA-seq
Profile of Podocyte Translatome During Development of Type 2 and Type 1 Diabetic Nephropathy Using Podocyte-Specific TRAP mRNA RNA-seq Open
Podocyte injury is important in development of diabetic nephropathy (DN). Although several studies have reported single-cell-based RNA sequencing (RNA-seq) of podocytes in type 1 DN (T1DN), the podocyte translating mRNA profile in type 2 D…
View article: Profile of Podocyte Translatome During Development of Type 2 and Type 1 Diabetic Nephropathy Using Podocyte-Specific TRAP mRNA RNA-seq
Profile of Podocyte Translatome During Development of Type 2 and Type 1 Diabetic Nephropathy Using Podocyte-Specific TRAP mRNA RNA-seq Open
Podocyte injury is important in development of diabetic nephropathy (DN). Although several studies have reported single cell-based RNA-seq of podocytes in type 1 DN (T1DN), the podocyte translating mRNA profile in type 2 DN (T2DN) has not …
View article: Profile of Podocyte Translatome During Development of Type 2 and Type 1 Diabetic Nephropathy Using Podocyte-Specific TRAP mRNA RNA-seq
Profile of Podocyte Translatome During Development of Type 2 and Type 1 Diabetic Nephropathy Using Podocyte-Specific TRAP mRNA RNA-seq Open
Podocyte injury is important in development of diabetic nephropathy (DN). Although several studies have reported single cell-based RNA-seq of podocytes in type 1 DN (T1DN), the podocyte translating mRNA profile in type 2 DN (T2DN) has not …
View article: LDL delivery of microbial small RNAs drives atherosclerosis through macrophage TLR8
LDL delivery of microbial small RNAs drives atherosclerosis through macrophage TLR8 Open
Macrophages present a spectrum of phenotypes that mediate both the pathogenesis and resolution of atherosclerotic lesions. Inflammatory macrophage phenotypes are pro-atherogenic, but the natural factors that instigate this polarization are…
View article: Biogenesis of RNA-containing extracellular vesicles at endoplasmic reticulum membrane contact sites
Biogenesis of RNA-containing extracellular vesicles at endoplasmic reticulum membrane contact sites Open
Summary RNA transferred via extracellular vesicles (EVs) can influence cell and tissue phenotypes; however, the biogenesis of RNA-containing EVs is poorly understood and even controversial. Here, we identify the conserved endoplasmic retic…
View article: Pervasive Small RNAs in Cardiometabolic Research: Great Potential Accompanied by Biological and Technical Barriers
Pervasive Small RNAs in Cardiometabolic Research: Great Potential Accompanied by Biological and Technical Barriers Open
Advances in small RNA sequencing have revealed the enormous diversity of small noncoding RNA (sRNA) classes in mammalian cells. At this point, most investigators in diabetes are aware of the success of microRNA (miRNA) research and appreci…
View article: The Endogenous Plasma Small RNAome of Rheumatoid Arthritis
The Endogenous Plasma Small RNAome of Rheumatoid Arthritis Open
Objective Small RNA ( sRNA ) sequencing has revealed new sRNA classes beyond micro RNA s (mi RNA s). These sRNA s can regulate genes and act as biomarkers. The aim of this study was to determine if the endogenous plasma sRNA landscape is a…
View article: Statins Stimulate Hepatic Glucose Production via the miR-183/96/182 Cluster
Statins Stimulate Hepatic Glucose Production via the miR-183/96/182 Cluster Open
Statins are the most common pharmacologic intervention in hypercholesterolemic patients, and their use is recognized as a key medical advance leading to a 50% decrease in deaths from heart attack or stroke over the past 30 years. The ather…
View article: Development and Validation of a MicroRNA Panel to Differentiate Between Patients with Rheumatoid Arthritis or Systemic Lupus Erythematosus and Controls
Development and Validation of a MicroRNA Panel to Differentiate Between Patients with Rheumatoid Arthritis or Systemic Lupus Erythematosus and Controls Open
Objective. MicroRNA (miRNA) are short noncoding RNA that regulate genes and are both biomarkers and mediators of disease. We used small RNA (sRNA) sequencing and machine learning methodology to develop an miRNA panel to reliably differenti…
View article: Transfer of Functional Cargo in Exomeres
Transfer of Functional Cargo in Exomeres Open
Exomeres are a recently discovered type of extracellular nanoparticle with no known biological function. Herein, we describe a simple ultracentrifugation-based method for separation of exomeres from exosomes. Exomeres are enriched in Argon…
View article: Intestinal bile acid sequestration improves glucose control by stimulating hepatic miR-182-5p in type 2 diabetes
Intestinal bile acid sequestration improves glucose control by stimulating hepatic miR-182-5p in type 2 diabetes Open
Colesevelam is a bile acid sequestrant approved to treat both hyperlipidemia and type 2 diabetes, but the mechanism for its glucose-lowering effects is not fully understood. The aim of this study was to investigate the role of hepatic micr…
View article: Bioinformatic analysis of endogenous and exogenous small RNAs on lipoproteins
Bioinformatic analysis of endogenous and exogenous small RNAs on lipoproteins Open
To comprehensively study extracellular small RNAs (sRNA) by sequencing (sRNA‐seq), we developed a novel pipeline to overcome current limitations in analysis entitled, “Tools for Integrative Genome analysis of Extracellular sRNAs (TIGER)”. …
View article: Bioinformatic analysis of endogenous and exogenous small RNAs on lipoproteins
Bioinformatic analysis of endogenous and exogenous small RNAs on lipoproteins Open
To comprehensively study extracellular small RNAs (sRNA) by sequencing (sRNA-seq), we developed a novel pipeline to overcome current limitations in analysis entitled, “ Tools for Integrative Genome analysis of Extracellular sRNAs (TIGER)”.…
View article: Isolation of High-density Lipoproteins for Non-coding Small RNA Quantification
Isolation of High-density Lipoproteins for Non-coding Small RNA Quantification Open
The diversity of small non-coding RNAs (sRNA) is rapidly expanding and their roles in biological processes, including gene regulation, are emerging. Most interestingly, sRNAs are also found outside of cells and are stably present in all bi…
View article: Stocking for a trophy bass fishery: searching for size differences among largemouth bass and hybrids in southern Arkansas reservoirs
Stocking for a trophy bass fishery: searching for size differences among largemouth bass and hybrids in southern Arkansas reservoirs Open
Lamothe KA, Allen RM, Winningham K, Dennis C, Johnson RL. 2016. Stocking for a trophy bass fishery: searching for size differences among largemouth bass and hybrids in southern Arkansas reservoirs. Lake Reserve Manage. 32:194–207.Within th…