Ryan C. Burdick
YOU?
Author Swipe
View article: Elucidating the mechanism by which HIV-1 nucleocapsid mutations confer resistance to integrase strand transfer inhibitors
Elucidating the mechanism by which HIV-1 nucleocapsid mutations confer resistance to integrase strand transfer inhibitors Open
Persons with HIV (PWH) receiving integrase (IN) strand transfer inhibitors (INSTIs) have been reported to experience virologic failure (VF) in the absence of resistance mutations in IN. We previously reported that mutations in the viral nu…
View article: The central pore of HIV-1 capsomers promotes sustained stability of the viral capsid
The central pore of HIV-1 capsomers promotes sustained stability of the viral capsid Open
The HIV-1 capsid, which orchestrates several key post-entry events to facilitate infection in target cells, is composed of hexamers and pentamers (capsomers) of the capsid (CA) protein arranged in a closed, conical structure known as the c…
View article: Elucidating the Mechanism by Which HIV-1 Nucleocapsid Mutations Confer Resistance to Integrase Strand Transfer Inhibitors
Elucidating the Mechanism by Which HIV-1 Nucleocapsid Mutations Confer Resistance to Integrase Strand Transfer Inhibitors Open
Persons with HIV (PWH) receiving integrase (IN) strand transfer inhibitors (INSTIs) have been reported to experience virologic failure (VF) in the absence of resistance mutations in IN. We previously reported that mutations in the viral nu…
View article: Lenacapavir-induced Lattice Hyperstabilization is Central to HIV-1 Capsid Failure at the Nuclear Pore Complex and in the Cytoplasm
Lenacapavir-induced Lattice Hyperstabilization is Central to HIV-1 Capsid Failure at the Nuclear Pore Complex and in the Cytoplasm Open
Lenacapavir (LEN) is the first HIV-1 capsid inhibitor approved for clinical use. It inhibits multiple steps of the viral life cycle; however, the molecular details of the effect of LEN on capsid structure and the mechanistic steps of the i…
View article: Lenacapavir disrupts HIV-1 core integrity while stabilizing the capsid lattice
Lenacapavir disrupts HIV-1 core integrity while stabilizing the capsid lattice Open
Lenacapavir (GS-6207; LEN) is a potent HIV-1 capsid inhibitor approved for treating multidrug-resistant infection. LEN binds to a hydrophobic pocket between neighboring capsid (CA) proteins in hexamers and stabilizes the capsid lattice, bu…
View article: HIV-1 uncoating requires long double-stranded reverse transcription products
HIV-1 uncoating requires long double-stranded reverse transcription products Open
HIV-1 cores, which contain the viral genome and replication machinery, must disassemble (uncoat) during viral replication. However, the viral and host factors that trigger uncoating remain unidentified. Recent studies show that infectious …
View article: The capsid lattice engages a bipartite NUP153 motif to mediate nuclear entry of HIV-1 cores
The capsid lattice engages a bipartite NUP153 motif to mediate nuclear entry of HIV-1 cores Open
Increasing evidence has suggested that the HIV-1 capsid enters the nucleus in a largely assembled, intact form. However, not much is known about how the cone-shaped capsid interacts with the nucleoporins (NUPs) in the nuclear pore for cros…
View article: Intranuclear Positions of HIV-1 Proviruses Are Dynamic and Do Not Correlate with Transcriptional Activity
Intranuclear Positions of HIV-1 Proviruses Are Dynamic and Do Not Correlate with Transcriptional Activity Open
HIV-1 integrates its genomic DNA into the chromosomes of the infected cell, but how it selects the site of integration and the impact of their location in the 3-dimensional nuclear space is not well understood. Here, we examined the nuclea…
View article: HIV-1 cores retain their integrity until minutes before uncoating in the nucleus
HIV-1 cores retain their integrity until minutes before uncoating in the nucleus Open
Significance Here, we used a fluorescent protein that is free in solution and is trapped in nuclear HIV-1 capsids to demonstrate that the capsids retain integrity and prevent mixing of macromolecules within the viral core and the cellular …
View article: Developing Entangled Two-Photon Absorption for Unique Control over Excitation of Organic Chromophores: An Experimental and Theoretical Approach
Developing Entangled Two-Photon Absorption for Unique Control over Excitation of Organic Chromophores: An Experimental and Theoretical Approach Open
By controlling quantum interactions of molecules and light, new technology in quantum information science promises to outperform classical-based technology in both function and efficiency. In particular, chemists stand to gain much advanta…
View article: Predicting and controlling entangled two-photon absorption in diatomic molecules
Predicting and controlling entangled two-photon absorption in diatomic molecules Open
The use of nonclassical\nstates of light to probe organic molecules\nhas received great attention due to the possibility of providing new\nand detailed information regarding molecular excitations. Experimental\nand theoretical results have…
View article: HIV-1 uncoats in the nucleus near sites of integration
HIV-1 uncoats in the nucleus near sites of integration Open
Significance For several decades, retroviral core uncoating has been thought to occur in the cytoplasm in coordination with reverse transcription, and while some recent studies have concluded that HIV-1 uncoating occurs at the nuclear enve…
View article: Insights into DNA substrate selection by APOBEC3G from structural, biochemical, and functional studies
Insights into DNA substrate selection by APOBEC3G from structural, biochemical, and functional studies Open
Human apolipoprotein B mRNA-editing enzyme-catalytic polypeptide-like 3 (A3) proteins are a family of cytidine deaminases that catalyze the conversion of deoxycytidine (dC) to deoxyuridine (dU) in single-stranded DNA (ssDNA). A3 proteins a…
View article: Insights into DNA substrate selection by APOBEC3G from structural, biochemical, and functional studies
Insights into DNA substrate selection by APOBEC3G from structural, biochemical, and functional studies Open
Human apolipoprotein B mRNA-editing enzyme-catalytic polypeptide-like 3 (A3) proteins are a family of cytidine deaminases that catalyze the conversion of cytidine to uridine in single-stranded DNA (ssDNA). A3 proteins act in the innate imm…
View article: Dynamics and regulation of nuclear import and nuclear movements of HIV-1 complexes
Dynamics and regulation of nuclear import and nuclear movements of HIV-1 complexes Open
The dynamics and regulation of HIV-1 nuclear import and its intranuclear movements after import have not been studied. To elucidate these essential HIV-1 post-entry events, we labeled viral complexes with two fluorescently tagged virion-in…
View article: Interactions between HIV-1 Gag and Viral RNA Genome Enhance Virion Assembly
Interactions between HIV-1 Gag and Viral RNA Genome Enhance Virion Assembly Open
Most HIV-1 virions contain two copies of full-length viral RNA, indicating that genome packaging is efficient and tightly regulated. However, the structural protein Gag is the only component required for the assembly of noninfectious virus…
View article: APOBEC3 proteins can copackage and comutate HIV-1 genomes
APOBEC3 proteins can copackage and comutate HIV-1 genomes Open
Although APOBEC3 cytidine deaminases A3G, A3F, A3D and A3H are packaged into virions and inhibit viral replication by inducing G-to-A hypermutation, it is not known whether they are copackaged and whether they can act additively or synergi…
View article: Minimal Contribution of APOBEC3-Induced G-to-A Hypermutation to HIV-1 Recombination and Genetic Variation
Minimal Contribution of APOBEC3-Induced G-to-A Hypermutation to HIV-1 Recombination and Genetic Variation Open
Although the predominant effect of host restriction APOBEC3 proteins on HIV-1 infection is to block viral replication, they might inadvertently increase retroviral genetic variation by inducing G-to-A hypermutation. Numerous studies have d…