Ryan Colbeth
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View article: Large-Scale Transgenic <i>Drosophila</i> Resource Collections for Loss- and Gain-of-Function Studies
Large-Scale Transgenic <i>Drosophila</i> Resource Collections for Loss- and Gain-of-Function Studies Open
The Transgenic RNAi Project (TRiP), a Drosophila melanogaster functional genomics platform at Harvard Medical School, was initiated in 2008 to generate and distribute a genome-scale collection of RNA interference (RNAi) fly stocks. To date…
View article: Large-scale transgenic<i>Drosophila</i>resource collections for loss- and gain-of-function studies
Large-scale transgenic<i>Drosophila</i>resource collections for loss- and gain-of-function studies Open
The Transgenic RNAi Project (TRiP), a Drosophila functional genomics platform at Harvard Medical School, was initiated in 2008 to generate and distribute a genome-scale collection of RNAi fly stocks. To date, the TRiP has generated >15,000…
View article: Gene Knock-Ins in <i>Drosophila</i> Using Homology-Independent Insertion of Universal Donor Plasmids
Gene Knock-Ins in <i>Drosophila</i> Using Homology-Independent Insertion of Universal Donor Plasmids Open
Targeted genomic knock-ins are a valuable tool to probe gene function. However, knock-in methods involving homology-directed repair (HDR) can be laborious. Here, we adapt the mammalian CRISPaint [clustered regularly interspaced short palin…
View article: Gene knock-ins in<i>Drosophila</i>using homology-independent insertion of universal donor plasmids
Gene knock-ins in<i>Drosophila</i>using homology-independent insertion of universal donor plasmids Open
Targeted genomic knock-ins are a valuable tool to probe gene function. However, knock-in methods involving homology-directed repair (HDR) can be laborious. Here, we adapt the mammalian CRISPaint homology-independent knock-in method for Dro…