Ryan Hicks
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View article: VHL Gene Restoration Supports RCC Reprogramming to iPSCs but Does Not Ensure Line Stability
VHL Gene Restoration Supports RCC Reprogramming to iPSCs but Does Not Ensure Line Stability Open
Background: Modeling precancerous stages holds the promise to understand early transformation events, thereby offering the potential for personalized, targeted treatment. Because cancer hijacks developmental pathways, precancerous stages c…
View article: Menstruation-induced vaso-occlusive crisis in women with sickle cell disease
Menstruation-induced vaso-occlusive crisis in women with sickle cell disease Open
Introduction: Sickle cell disease (SCD) affects approximately 100,000 people in the United States. Pain is a well-known complication of the disease, with vaso-occlusive crisis (VOC) being a common reason for patients to seek acute care. Pa…
View article: A human induced pluripotent stem cell toolbox for studying sex chromosome effects
A human induced pluripotent stem cell toolbox for studying sex chromosome effects Open
Sex chromosomes shape male (XY)-female (XX) differences in development and disease. These differences can be modeled in vitro by comparing XY and XX human induced pluripotent stem cells (hiPSCs). However, in this system, inter-individual a…
View article: A highly efficient method to differentiate CGRP-expressing peptidergic nociceptors from human induced pluripotent stem cells
A highly efficient method to differentiate CGRP-expressing peptidergic nociceptors from human induced pluripotent stem cells Open
Human cellular models of disease developed using induced pluripotent stem cells (iPSC) have enabled wide-ranging research from investigation of human disease mechanisms to phenotypic drug screens. Pain disorders such as neuropathic pain an…
View article: A human induced pluripotent stem cell toolbox for studying sex chromosome effects
A human induced pluripotent stem cell toolbox for studying sex chromosome effects Open
Summary Sex chromosomes shape male (XY) - female (XX) differences in development and disease. These differences can be modelled in vitro by comparing XY and XX human induced pluripotent stem cells (hiPSCs). However, in this system, inter-i…
View article: Microfluidic vessel-on-chip platform for investigation of cellular defects in venous malformations and responses to various shear stress and flow conditions
Microfluidic vessel-on-chip platform for investigation of cellular defects in venous malformations and responses to various shear stress and flow conditions Open
In-line shear stress modulation and cell microscopic analysis.
View article: Cryopreserved apoptotic mesenchymal stromal cells retain functional efficacy in suppressing an allergic inflammation in a murine model
Cryopreserved apoptotic mesenchymal stromal cells retain functional efficacy in suppressing an allergic inflammation in a murine model Open
Mesenchymal stromal cell (MSC) apoptosis is required for in vivo immunosuppression. However, the induction of apoptosis is heavily dependent on the recipient’s immune system. In graft-versus-host disease (GvHD), patients who fail to respon…
View article: RYR2 deficient human model identifies calcium handling and metabolic dysfunction impacting pharmacological responses
RYR2 deficient human model identifies calcium handling and metabolic dysfunction impacting pharmacological responses Open
Creation of disease models utilizing hiPSCs in combination with CRISPR/Cas9 gene editing enable mechanistic insights into differential pharmacological responses. This allows translation of efficacy and safety findings from a healthy to a d…
View article: Human iPSC and CRISPR targeted gene knock-in strategy for studying the somatic TIE2L914F mutation in endothelial cells
Human iPSC and CRISPR targeted gene knock-in strategy for studying the somatic TIE2L914F mutation in endothelial cells Open
Induced pluripotent stem cell (iPSC) derived endothelial cells (iECs) have emerged as a promising tool for studying vascular biology and providing a platform for modelling various vascular diseases, including those with genetic origins. Cu…
View article: Issue Information
Issue Information Open
Family Process Institute is an independent, multidisciplinary, transnational organization dedicated to developing and exchanging new theory, research, applied practice, and policy related to families and other systems.Grounded in a commitm…
View article: APOL1 promotes endothelial cell activation beyond the glomerulus
APOL1 promotes endothelial cell activation beyond the glomerulus Open
Apolipoprotein L1 (APOL1) high-risk genotypes are associated with increased risk of chronic kidney disease (CKD) in people of West African ancestry. Given the importance of endothelial cells (ECs) in CKD, we hypothesized that APOL1 high-ri…
View article: The critical role of apoptosis in mesenchymal stromal cell therapeutics and implications in homeostasis and normal tissue repair
The critical role of apoptosis in mesenchymal stromal cell therapeutics and implications in homeostasis and normal tissue repair Open
Mesenchymal stromal cells (MSCs) have been extensively tested for the treatment of numerous clinical conditions and have demonstrated good safety but mixed efficacy. Although this outcome can be attributed in part to the heterogeneity of c…
View article: 3D vascularised proximal tubules-on-a-multiplexed chip model for enhanced cell phenotypes
3D vascularised proximal tubules-on-a-multiplexed chip model for enhanced cell phenotypes Open
Incremental increases in complexity in a model of the vascularised proximal tubule give rise to increased maturity and improved physiology.
View article: Saracatinib, a Selective Src Kinase Inhibitor, Blocks Fibrotic Responses in Preclinical Models of Pulmonary Fibrosis
Saracatinib, a Selective Src Kinase Inhibitor, Blocks Fibrotic Responses in Preclinical Models of Pulmonary Fibrosis Open
Rationale: Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive, and often fatal disorder. Two U.S. Food and Drug Administration-approved antifibrotic drugs, nintedanib and pirfenidone, slow the rate of decline in lung function, b…
View article: Proof-of-Concept of a Novel Cell Separation Technology Using Magnetic Agarose-Based Beads
Proof-of-Concept of a Novel Cell Separation Technology Using Magnetic Agarose-Based Beads Open
The safety of the cells used for Advanced Therapy Medicinal Products is crucial for patients. Reliable methods for the cell purification are very important for the commercialization of those new therapies. With the large production scale e…
View article: Saracatinib, a Selective Src Kinase Inhibitor, Blocks Fibrotic Responses in <i>In Vitro, In Vivo</i> and <i>Ex Vivo</i> Models of Pulmonary Fibrosis
Saracatinib, a Selective Src Kinase Inhibitor, Blocks Fibrotic Responses in <i>In Vitro, In Vivo</i> and <i>Ex Vivo</i> Models of Pulmonary Fibrosis Open
Idiopathic Pulmonary Fibrosis (IPF) is a chronic, progressive, and often fatal disorder. Two FDA approved anti-fibrotic drugs, nintedanib and pirfenidone, slow the rate of decline in lung function, but responses are variable and side effec…
View article: Repositioning of a Novel GABA-B Receptor Agonist, AZD3355&nbsp;(Lesogaberan), for the Treatment of Non-alcoholic Steatohepatitis
Repositioning of a Novel GABA-B Receptor Agonist, AZD3355 (Lesogaberan), for the Treatment of Non-alcoholic Steatohepatitis Open
Non-alcoholic steatohepatitis (NASH) is a rising health challenge, with no approved drugs. We used a computational drug repositioning strategy to uncover a novel therapy for NASH, identifying a GABA-B receptor agonist, AZD3355 (lesogaberan…
View article: Umbilical Cord Tissue as a Source of Young Cells for the Derivation of Induced Pluripotent Stem Cells Using Non-Integrating Episomal Vectors and Feeder-Free Conditions
Umbilical Cord Tissue as a Source of Young Cells for the Derivation of Induced Pluripotent Stem Cells Using Non-Integrating Episomal Vectors and Feeder-Free Conditions Open
The clinical application of induced pluripotent stem cells (iPSC) needs to balance the use of an autologous source that would be a perfect match for the patient against any safety or efficacy issues that might arise with using cells from a…
View article: Optimised generation of iPSC-derived macrophages and dendritic cells that are functionally and transcriptionally similar to their primary counterparts
Optimised generation of iPSC-derived macrophages and dendritic cells that are functionally and transcriptionally similar to their primary counterparts Open
Induced pluripotent stem cells (iPSC) offer the possibility to generate diverse disease-relevant cell types, from any genetic background with the use of cellular reprogramming and directed differentiation. This provides a powerful platform…
View article: Models of the blood-brain barrier using iPSC-derived cells
Models of the blood-brain barrier using iPSC-derived cells Open
The blood-brain barrier (BBB) constitutes the interface between the blood and the brain tissue. Its primary function is to maintain the tightly controlled microenvironment of the brain. Models of the BBB are useful for studying the develop…
View article: hiPS‐Derived Astroglia Model Shows Temporal Transcriptomic Profile Related to Human Neural Development and Glia Competence Acquisition of a Maturing Astrocytic Identity
hiPS‐Derived Astroglia Model Shows Temporal Transcriptomic Profile Related to Human Neural Development and Glia Competence Acquisition of a Maturing Astrocytic Identity Open
Astrocyte biology has a functional and cellular diversity only observed in humans. The understanding of the regulatory network governing outer radial glia (RG), responsible for the expansion of the outer subventricular zone (oSVZ), and ast…