Ryan McQuillen
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View article: Light-dependent modulation of protein localization and function in living bacteria cells
Light-dependent modulation of protein localization and function in living bacteria cells Open
Most bacteria lack membrane-enclosed organelles and rely on macromolecular scaffolds at different subcellular locations to recruit proteins for specific functions. Here, we demonstrate that the optogenetic CRY2-CIB1 system from Arabidopsis…
DeepPaint: A deep-learning package for Cell Painting Image Classification Open
Recent developments in the high-content imaging (HCI) space have allowed for the production of large Cell Painting datasets. These datasets are typically derived from cells exposed to a set of biological perturbants including proteins, sma…
View article: Light-dependent modulation of protein localization and function in living bacteria cells
Light-dependent modulation of protein localization and function in living bacteria cells Open
Most bacteria lack membrane-enclosed organelles to compartmentalize cellular processes. In lieu of physical compartments, bacterial proteins are often recruited to macromolecular scaffolds at specific subcellular locations to carry out the…
View article: FtsN maintains active septal cell wall synthesis by forming a processive complex with the septum-specific peptidoglycan synthases in E. coli
FtsN maintains active septal cell wall synthesis by forming a processive complex with the septum-specific peptidoglycan synthases in E. coli Open
FtsN plays an essential role in promoting the inward synthesis of septal peptidoglycan (sPG) by the FtsWI complex during bacterial cell division. How it achieves this role is unclear. Here we use single-molecule tracking to investigate Fts…
View article: Light-dependent modulation of protein localization and function in living bacteria cells
Light-dependent modulation of protein localization and function in living bacteria cells Open
Most bacteria lack membrane-enclosed organelles to compartmentalize cellular processes. In lieu of physical compartments, bacterial proteins are often recruited to macromolecular scaffolds at specific subcellular locations to carry out the…
View article: FtsN activates septal cell wall synthesis by forming a processive complex with the septum-specific peptidoglycan synthase in <i>E. coli</i>
FtsN activates septal cell wall synthesis by forming a processive complex with the septum-specific peptidoglycan synthase in <i>E. coli</i> Open
The FtsN protein of Escherichia coli and other proteobacteria is an essential and highly conserved bitopic membrane protein that triggers the inward synthesis of septal peptidoglycan (sPG) during cell division. Previous work has shown that…
Insights into the Structure, Function, and Dynamics of the Bacterial Cytokinetic FtsZ-Ring Open
The FtsZ protein is a highly conserved bacterial tubulin homolog. In vivo, the functional form of FtsZ is the polymeric, ring-like structure (Z-ring) assembled at the future division site during cell division. While it is clear that the Z-…
View article: FtsW exhibits distinct processive motions driven by either septal cell wall synthesis or FtsZ treadmilling in <i>E. coli</i>
FtsW exhibits distinct processive motions driven by either septal cell wall synthesis or FtsZ treadmilling in <i>E. coli</i> Open
During bacterial cell division, synthesis of new septal peptidoglycan (sPG) is crucial for successful cytokinesis and cell pole morphogenesis. FtsW, a SEDS (Shape, Elongation, Division and Sporulation) family protein and an indispensable c…
A conserved coiled‐coil protein pair focuses the cytokinetic Z‐ring in <i>Caulobacter crescentus</i> Open
Summary The cytoskeletal GTPase FtsZ assembles at midcell, recruits the division machinery and directs envelope invagination for bacterial cytokinesis. ZapA, a conserved FtsZ‐binding protein, promotes Z‐ring stability and efficient divisio…
GTPase activity–coupled treadmilling of the bacterial tubulin FtsZ organizes septal cell wall synthesis Open
Coordinating cell wall synthesis and cell division Most bacteria are protected by peptidoglycan cell walls, which must be remodeled to split the cell. Cell division requires the tubulin homolog FtsZ, a highly conserved cytoskeletal polymer…
GTPase activity-coupled treadmilling of the bacterial tubulin FtsZ organizes septal cell-wall synthesis Open
The bacterial tubulin FtsZ is the central component of the division machinery, coordinating an ensemble of proteins involved in septal cell-wall synthesis to ensure successful constriction. How cells achieve this coordination is unknown. W…