Ryan Simkovsky
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View article: Sustainable production of plastic-degrading enzymes in <i>Chlamydomonas pacifica</i>
Sustainable production of plastic-degrading enzymes in <i>Chlamydomonas pacifica</i> Open
The discovery of a new extremophile alga, Chlamydomonas pacifica , provides an opportunity to expand on heterologous protein expression beyond the traditional Chlamydomonas reinhardtii. C. pacifica is a unicellular extremophile capable of …
View article: Mechanically Robust and Biodegradable Electrospun Membranes Made from Bioderived Thermoplastic Polyurethane and Polylactic Acid
Mechanically Robust and Biodegradable Electrospun Membranes Made from Bioderived Thermoplastic Polyurethane and Polylactic Acid Open
Petroleum-based plastic waste plagues the natural environment, but plastics solve many high-performance solutions across industries. For example, porous polymer membranes are used for air filtration, advanced textiles, energy, and biomedic…
View article: Rapid biodegradation of microplastics generated from bio-based thermoplastic polyurethane
Rapid biodegradation of microplastics generated from bio-based thermoplastic polyurethane Open
The accumulation of microplastics in various ecosystems has now been well documented and recent evidence suggests detrimental effects on various biological processes due to this pollution. Accumulation of microplastics in the natural envir…
View article: Renewable and Biodegradable Polyurethane Foams with Aliphatic Diisocyanates
Renewable and Biodegradable Polyurethane Foams with Aliphatic Diisocyanates Open
The transition to renewable plastics will require the development of substitutes with existing industrial standards and manufacturing processes. Polyurethanes (PU), versatile plastics, are traditionally dominated by aromatic diisocyanates,…
View article: Advanced Algal Biofoundries for the Production of Polyurethane Precursors
Advanced Algal Biofoundries for the Production of Polyurethane Precursors Open
The primary goal of the BEEPs project was to develop a process that could accelerate the development of algae as bioproduction platforms, from initial chemical product concept to an economically viable market supply. Under this program we …
View article: Utilization of lignocellulosic hydrolysates for photomixotrophic chemical production in Synechococcus elongatus PCC 7942
Utilization of lignocellulosic hydrolysates for photomixotrophic chemical production in Synechococcus elongatus PCC 7942 Open
To meet the need for environmentally friendly commodity chemicals, feedstocks for biological chemical production must be diversified. Lignocellulosic biomass are an carbon source with the potential for effective use in a large scale and co…
View article: Biodegradable waterborne polyurethane‐urea dispersion adhesives with high biocontent
Biodegradable waterborne polyurethane‐urea dispersion adhesives with high biocontent Open
Non‐biodegradable petroleum‐based plastic wastes have become a leading environmental concern, and new efforts are underway to prepare biobased and biodegradable replacements. We have explored the preparation of adhesives suitable for use i…
View article: Transcriptomic and Phenomic Investigations Reveal Elements in Biofilm Repression and Formation in the Cyanobacterium Synechococcus elongatus PCC 7942
Transcriptomic and Phenomic Investigations Reveal Elements in Biofilm Repression and Formation in the Cyanobacterium Synechococcus elongatus PCC 7942 Open
Biofilm formation by photosynthetic organisms is a complex behavior that serves multiple functions in the environment. Biofilm formation in the unicellular cyanobacterium Synechococcus elongatus PCC 7942 is regulated in part by a set of sm…
View article: Comparative Genomics of Synechococcus elongatus Explains the Phenotypic Diversity of the Strains
Comparative Genomics of Synechococcus elongatus Explains the Phenotypic Diversity of the Strains Open
Synechococcus elongatus is a versatile and robust model cyanobacterium for photosynthetic metabolism and circadian biology research, with utility as a biological production platform. We compared the genomes of closely related S. elongatus …
View article: Integrated Pest Management (IPM) for Early Detection Algal Crop Protection. Final Report
Integrated Pest Management (IPM) for Early Detection Algal Crop Protection. Final Report Open
During large-scale, outdoor algal biomass growth, production strain(s) are subject to attack by pathogens, predators, and non-productive competitors, compromising the biomass yield. The project team at UCSD developed and demonstrated the e…
View article: Transcriptomic and phenomic investigations reveal elements in biofilm repression and formation in the cyanobacterium<i>Synechococcus elongatus</i>PCC 7942
Transcriptomic and phenomic investigations reveal elements in biofilm repression and formation in the cyanobacterium<i>Synechococcus elongatus</i>PCC 7942 Open
Biofilm formation by photosynthetic organisms is a complex behavior that serves multiple functions in the environment. Biofilm formation in the unicellular cyanobacterium Synechococcus elongatus PCC 7942 is regulated in part by a set of sm…
View article: Recombinant production of a functional SARS-CoV-2 spike receptor binding domain in the green algae Chlamydomonas reinhardtii
Recombinant production of a functional SARS-CoV-2 spike receptor binding domain in the green algae Chlamydomonas reinhardtii Open
Recombinant production of viral proteins can be used to produce vaccine antigens or reagents to identify antibodies in patient serum. Minimally, these proteins must be correctly folded and have appropriate post-translation modifications. H…
View article: Recombinant production of a functional SARS-CoV-2 spike receptor binding domain in the green algae Chlamydomonas reinhardtii
Recombinant production of a functional SARS-CoV-2 spike receptor binding domain in the green algae Chlamydomonas reinhardtii Open
Recombinant production of viral proteins can be used to produce vaccine antigens or reagents to identify antibodies in patient serum. Minimally, these proteins must be correctly folded and have appropriate post-translation modifications. H…
View article: Continuous measurements of volatile gases as detection of algae crop health
Continuous measurements of volatile gases as detection of algae crop health Open
Significance Wide adoption of algae cultivation to produce environmentally sustainable biofuels and fine chemicals is currently hampered by large losses (10 to 30%) incurred by grazer infections. We show the usage of real-time chemical ion…
View article: A Cyanobacterial Component Required for Pilus Biogenesis Affects the Exoproteome
A Cyanobacterial Component Required for Pilus Biogenesis Affects the Exoproteome Open
Cyanobacteria, environmentally prevalent photosynthetic prokaryotes, contribute ∼25% of global primary production. Cyanobacterial biofilms elicit biofouling, thus leading to substantial economic losses; however, these microbial assemblages…
View article: Heterologous Expression of Cryptomaldamide in a Cyanobacterial Host
Heterologous Expression of Cryptomaldamide in a Cyanobacterial Host Open
Filamentous marine cyanobacteria make a variety of bioactive molecules that are produced by polyketide synthases, nonribosomal peptide synthetases, and hybrid pathways that are encoded by large biosynthetic gene clusters. These cyanobacter…
View article: Heterologous expression of cryptomaldamide in a cyanobacterial host
Heterologous expression of cryptomaldamide in a cyanobacterial host Open
Filamentous marine cyanobacteria make a variety of bioactive molecules that are produced by polyketide synthases, non-ribosomal peptide synthetases, and hybrid pathways that are encoded by large biosynthetic gene clusters. These cyanobacte…
View article: Rapid biodegradation of renewable polyurethane foams with identification of associated microorganisms and decomposition products
Rapid biodegradation of renewable polyurethane foams with identification of associated microorganisms and decomposition products Open
The goal of this research was to determine if we could develop commercially-relevant polyurethane products that can biodegrade in the natural environment. We developed polyester polyols from algae oils and formulated those into polyurethan…
View article: Phototaxis in a wild isolate of the cyanobacterium <i>Synechococcus elongatus</i>
Phototaxis in a wild isolate of the cyanobacterium <i>Synechococcus elongatus</i> Open
Many cyanobacteria, which use light as an energy source via photosynthesis, have evolved the ability to guide their movement toward or away from a light source. This process, termed “phototaxis,” enables organisms to localize in optimal li…
View article: High-throughput interaction screens illuminate the role of c-di-AMP in cyanobacterial nighttime survival
High-throughput interaction screens illuminate the role of c-di-AMP in cyanobacterial nighttime survival Open
The broadly conserved signaling nucleotide cyclic di-adenosine monophosphate (c-di-AMP) is essential for viability in most bacteria where it has been studied. However, characterization of the cellular functions and metabolism of c-di-AMP h…
View article: Quantification of Chlorophyll as a Proxy for Biofilm Formation in the Cyanobacterium Synechococcus elongatus
Quantification of Chlorophyll as a Proxy for Biofilm Formation in the Cyanobacterium Synechococcus elongatus Open
\nA self-suppression mechanism of biofilm development in the cyanobacterium Synechococcus elongatus PCC 7942 was recently reported. These studies required quantification of biofilms formed by mutants impaired in the biofilm-inhibitory proc…
View article: Mutations in Novel Lipopolysaccharide Biogenesis Genes Confer Resistance to Amoebal Grazing in Synechococcus elongatus
Mutations in Novel Lipopolysaccharide Biogenesis Genes Confer Resistance to Amoebal Grazing in Synechococcus elongatus Open
In natural and artificial aquatic environments, population structures and dynamics of photosynthetic microbes are heavily influenced by the grazing activity of protistan predators. Understanding the molecular factors that affect predation …