Ryan L. Slack
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View article: Translocation of HIV capsid core through the Nuclear Pore Complex by affinity gradient
Translocation of HIV capsid core through the Nuclear Pore Complex by affinity gradient Open
SUMMARY The HIV capsid core encapsulates the viral genome for subsequent integration into host cellular DNA. Prior to nuclear entry, the core must translocate through the Nuclear Pore Complex (NPC). This transit involves interactions betwe…
View article: Synthesis and Antiviral Evaluation of Unexplored Dioxolane-Derived 7-Deazapurine Nucleoside Analogues against Epstein–Barr Virus (EBV)
Synthesis and Antiviral Evaluation of Unexplored Dioxolane-Derived 7-Deazapurine Nucleoside Analogues against Epstein–Barr Virus (EBV) Open
Dioxolane-based nucleosides are characterized by their unique replacement of the sugar moiety by dioxolane-based cyclopentyl rings. A series of d-dioxolane-derived 7-deazapurine analogues (12-19) were synthesized and evaluated against Epst…
View article: Characterization of antiviral compounds using Bio-Layer Interferometry
Characterization of antiviral compounds using Bio-Layer Interferometry Open
Small molecule-protein interactions underpin many biological functions and play an integral role in the treatment and prevention of several human diseases. These interactions can be key to understanding the mechanism of action of these com…
View article: Strategy to overcome a nirmatrelvir resistance mechanism in the SARS-CoV-2 nsp5 protease
Strategy to overcome a nirmatrelvir resistance mechanism in the SARS-CoV-2 nsp5 protease Open
E166V in the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nsp5 protease confers strong resistance to the antiviral component of Paxlovid, nirmatrelvir (NIR), in passaging and clinical samples. In SARS-CoV-2 replicons, E166V…
View article: A Tag‐Free Platform for Synthesis and Screening of Cyclic Peptide Libraries
A Tag‐Free Platform for Synthesis and Screening of Cyclic Peptide Libraries Open
In the realm of high‐throughput screening (HTS), macrocyclic peptide libraries traditionally necessitate decoding tags, essential for both library synthesis and identifying hit peptide sequences post‐screening. Our innovation introduces a …
View article: Nirmatrelvir Resistance in SARS-CoV-2 Omicron_BA.1 and WA1 Replicons and Escape Strategies
Nirmatrelvir Resistance in SARS-CoV-2 Omicron_BA.1 and WA1 Replicons and Escape Strategies Open
The antiviral component of Paxlovid, nirmatrelvir (NIR), forms a covalent bond with Cys145 of SARS-CoV-2 nsp5. To explore NIR resistance we designed mutations to impair binding of NIR over substrate. Using 12 Omicron (BA.1) and WA.1 SARS-C…
View article: Biochemical and structural insights into SARS-CoV-2 polyprotein processing by Mpro
Biochemical and structural insights into SARS-CoV-2 polyprotein processing by Mpro Open
SARS-CoV-2, a human coronavirus, is the causative agent of the COVID-19 pandemic. Its genome is translated into two large polyproteins subsequently cleaved by viral papain-like protease and main protease (Mpro). Polyprotein processing is e…
View article: Adaptation of HIV-1/HIV-2 Chimeras with Defects in Genome Packaging and Viral Replication
Adaptation of HIV-1/HIV-2 Chimeras with Defects in Genome Packaging and Viral Replication Open
Novel retroviruses can emerge from recombination between distantly related retroviruses. Most notably, HIV-1 originated from zoonotic transmission of a novel recombinant (SIV cpz ) into humans.
View article: Biochemical and structural insights into SARS-CoV-2 polyprotein processing by Mpro
Biochemical and structural insights into SARS-CoV-2 polyprotein processing by Mpro Open
SARS-CoV-2, a human coronavirus, is the causative agent of the COVID-19 pandemic. Its ∼30 kb RNA genome is translated into two large polyproteins subsequently cleaved by viral papain-like protease and main protease (Mpro/nsp5). Polyprotein…
View article: Subgenomic SARS-CoV-2 replicon and reporter replicon cell lines enable ultrahigh throughput antiviral screening and mechanistic studies with antivirals, viral mutations or host factors that affect COVID-19 replication
Subgenomic SARS-CoV-2 replicon and reporter replicon cell lines enable ultrahigh throughput antiviral screening and mechanistic studies with antivirals, viral mutations or host factors that affect COVID-19 replication Open
Replicon-based technologies were used to develop reagents and assays for advanced drug discovery efforts against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), and for examining all facets of the SARS-CoV-2 replication cycle…
View article: Baicalein and Baicalin Inhibit SARS-CoV-2 RNA-Dependent-RNA Polymerase
Baicalein and Baicalin Inhibit SARS-CoV-2 RNA-Dependent-RNA Polymerase Open
Coronavirus Disease 2019 (COVID-19) is a deadly emerging infectious disease caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). Because SARS-CoV-2 is easily transmitted through the air and has a relatively long incubati…
View article: Effect of Lysyl–tRNA Synthetase on the Maturation of HIV-1 Reverse Transcriptase
Effect of Lysyl–tRNA Synthetase on the Maturation of HIV-1 Reverse Transcriptase Open
In human immunodeficiency virus-1 (HIV-1), reverse transcriptase (RT) is encoded as a 66 kDa protein, p66, in the Gag-Pol polyprotein. This protein is proteolytically cleaved by HIV-1 protease (PR) to finally generate a mature RT that is a…