Ryohei Ueno
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View article: A CRISPR/Cas9-induced restoration of bioluminescence reporter system for single-cell gene expression analysis in plants
A CRISPR/Cas9-induced restoration of bioluminescence reporter system for single-cell gene expression analysis in plants Open
View article: A CRISPR/Cas9-induced restoration of bioluminescence reporter system for single-cell gene expression analysis in plants
A CRISPR/Cas9-induced restoration of bioluminescence reporter system for single-cell gene expression analysis in plants Open
Bioluminescence monitoring techniques have greatly contributed to revealing a variety of biological regulatory systems in living organisms, including circadian clocks. In plant science, these techniques are applied to long-term quantitativ…
View article: Cells sorted off hiPSC-derived kidney organoids coupled with immortalized cells reliably model the proximal tubule
Cells sorted off hiPSC-derived kidney organoids coupled with immortalized cells reliably model the proximal tubule Open
View article: Relationship between Adsorption and Toxicity of Nephrotoxic Drugs in Microphysiological Systems (MPS)
Relationship between Adsorption and Toxicity of Nephrotoxic Drugs in Microphysiological Systems (MPS) Open
Microphysiological systems (MPS) are an emerging technology for next-generation drug screening in non-clinical tests. Microphysiological systems are microfluidic devices that reconstitute the physiological functions of a human organ using …
View article: Biochemical microsensor array with structural color hydrogel for rapid target detection
Biochemical microsensor array with structural color hydrogel for rapid target detection Open
This paper describes a biochemical microsensor with arrayed structural color hydrogels enabling the rapid detection. We fabricated thermo-responsive structural color hydrogels composed of silica colloidal particles and N-isopropylacrylamid…
View article: Boyden chamber-based compartmentalized tumor spheroid culture system to implement localized anticancer drug treatment
Boyden chamber-based compartmentalized tumor spheroid culture system to implement localized anticancer drug treatment Open
In anticancer drug development, it is important to simultaneously evaluate both the effect of drugs on cell proliferation and their ability to penetrate tissues. To realize such an evaluation process, here, we present a compartmentalized t…
View article: Medical phycology 2017
Medical phycology 2017 Open
In 2014, ISHAM formed a new working group: "Medical Phycology: Protothecosis and Chlorellosis." The purpose of this working group is to help facilitate collaboration and communication among people interested in the pathogenic algae, to sha…
View article: Synthesis and herbicidal activity of 3-{[(hetero)aryl]methanesulfonyl}-4,5-dihydro-1,2-oxazole derivative; Discovery of the novel pre-emergence herbicide pyroxasulfone
Synthesis and herbicidal activity of 3-{[(hetero)aryl]methanesulfonyl}-4,5-dihydro-1,2-oxazole derivative; Discovery of the novel pre-emergence herbicide pyroxasulfone Open
Thiocarbamate sulfoxides, which are the active forms of thiocarbamate herbicides, are quickly conjugated with glutathione and decomposed in soil. To achieve more potent and stable herbicidal activity, we previously developed a 5-{[(2,6-dif…
View article: Electrical Property of Platinum Micro Heater for Thermal Analysis of Microfluidic Device
Electrical Property of Platinum Micro Heater for Thermal Analysis of Microfluidic Device Open
This paper shows thermal measurement and analysis of a microfluidic device integrated with local thin film heater. Thermal analysis was calculated by finite element modeling. Electrical property of the micro heater was calibrated in advanc…
View article: MoP-23 FABRICATION OF EMBEDDED GOLD MICRO ELECTRODE WITH PI IN PDMS SUBSTRATE USING MODIFIED DRY PEEL-OFF PROCESS
MoP-23 FABRICATION OF EMBEDDED GOLD MICRO ELECTRODE WITH PI IN PDMS SUBSTRATE USING MODIFIED DRY PEEL-OFF PROCESS Open
The present paper describes a modified dry peel-off process to fabricate stable gold micro electrode with polyimide (PI) embedded in a polydimethlysiloxane (PDMS) substrate. three kinds of self-assembled monolayer (SAM) were employed to co…
View article: MoP-22 DETECTION OF MALARIA USING SINGLE CELL TRAP MICROFLUIDIC DEVICE WITH FAST IMAGING
MoP-22 DETECTION OF MALARIA USING SINGLE CELL TRAP MICROFLUIDIC DEVICE WITH FAST IMAGING Open
This paper reports a simple and disposable polymer microwells chip to investigate single cell level of Red Blood Cells (RBCs) in parallel analysis with fast imaging. This method is based on the image processing method and microfluidic devi…