Ryosuke Saigusa
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View article: Combined protein and transcript single-cell RNA sequencing in human peripheral blood mononuclear cells
Combined protein and transcript single-cell RNA sequencing in human peripheral blood mononuclear cells Open
Background: Cryopreserved peripheral blood mononuclear cells (PBMCs) are frequently collected and provide disease- and treatment-relevant data in clinical studies. Here, we developed combined protein (40 antibodies) and transcript single-c…
View article: Single cell transcriptomics reveals recent CD8T cell receptor signaling in patients with coronary artery disease
Single cell transcriptomics reveals recent CD8T cell receptor signaling in patients with coronary artery disease Open
Coronary artery disease (CAD) is a major cause of death worldwide. The role of CD8+ T cells in CAD is unknown. Recent studies suggest a breakdown of tolerance in atherosclerosis, resulting in active T cell receptor (TCR) engagement with se…
View article: Single-cell profiling reveals inflammatory polarization of human carotid versus femoral plaque leukocytes
Single-cell profiling reveals inflammatory polarization of human carotid versus femoral plaque leukocytes Open
Femoral atherosclerotic plaques are less inflammatory than carotid plaques histologically, but limited cell-level data exist regarding comparative immune landscapes and polarization at these sites. We investigated intraplaque leukocyte phe…
View article: Single cell transcriptomic changes of CD8T cells in male and female patients with coronary artery disease and diabetes
Single cell transcriptomic changes of CD8T cells in male and female patients with coronary artery disease and diabetes Open
Objective The objective of this study was to determine the role of CD8+ T cells in subjects with coronary artery disease (CAD) with and without diabetes (DM). Methods PBMCs of 60 men and women who underwent cardiac catheterization were int…
View article: Combined protein and transcript single-cell RNA sequencing in human peripheral blood mononuclear cells
Combined protein and transcript single-cell RNA sequencing in human peripheral blood mononuclear cells Open
Background Cryopreserved peripheral blood mononuclear cells (PBMCs) are frequently collected and provide disease- and treatment-relevant data in clinical studies. Here, we developed combined protein (40 antibodies) and transcript single-ce…
View article: Sex Differences in Coronary Artery Disease and Diabetes Revealed by scRNA-Seq and CITE-Seq of Human CD4+ T Cells
Sex Differences in Coronary Artery Disease and Diabetes Revealed by scRNA-Seq and CITE-Seq of Human CD4+ T Cells Open
Despite the decades-old knowledge that males and people with diabetes mellitus (DM) are at increased risk for coronary artery disease (CAD), the reasons for this association are only partially understood. Among the immune cells involved, r…
View article: Sex differences in coronary artery disease and diabetes revealed by scRNA-Seq and CITE-Seq of human CD4+ T cells
Sex differences in coronary artery disease and diabetes revealed by scRNA-Seq and CITE-Seq of human CD4+ T cells Open
Background Despite the decades-old knowledge that diabetes mellitus (DM) is a major risk factor for cardiovascular disease (CVD), the reasons for this association are only partially understood. Among the immune cells involved in CVD develo…
View article: Chemokine Receptor Activation Enhances Memory B Cell Class Switching Linked to IgE Sensitization to Alpha Gal and Cardiovascular Disease
Chemokine Receptor Activation Enhances Memory B Cell Class Switching Linked to IgE Sensitization to Alpha Gal and Cardiovascular Disease Open
Background: Recent studies have suggested that IgE sensitization to α-gal is associated with coronary artery disease (CAD). However, the B cell subtype(s) responsible for production of IgE to α-gal and mechanisms mediating this production …
View article: Autoimmune Regulator (AIRE) Deficiency Does Not Affect Atherosclerosis and CD4 T Cell Immune Tolerance to Apolipoprotein B
Autoimmune Regulator (AIRE) Deficiency Does Not Affect Atherosclerosis and CD4 T Cell Immune Tolerance to Apolipoprotein B Open
Atherosclerosis is a chronic, lipid-driven disease of medium sized arteries which causes myocardial infarction and stroke. Recently, an adaptive immune response against the plaque-associated autoantigen Apolipoprotein B100 (ApoB), the stru…
View article: Additional file 3 of Combined protein and transcript single-cell RNA sequencing in human peripheral blood mononuclear cells
Additional file 3 of Combined protein and transcript single-cell RNA sequencing in human peripheral blood mononuclear cells Open
Additional file 3: Data S2. (separate excel file). Data underlying Figure 6, random forest model.
View article: Additional file 2 of Combined protein and transcript single-cell RNA sequencing in human peripheral blood mononuclear cells
Additional file 2 of Combined protein and transcript single-cell RNA sequencing in human peripheral blood mononuclear cells Open
Additional file 2: Data S1. (separate excel file). Data underlying Figure 5, dotplots of DEGs for disease types.
View article: Prognostic Relevance of Pretreatment Peripheral Neutrophil Count and Neutrophil-to-lymphocyte Ratio in Primary Cutaneous Angiosarcoma
Prognostic Relevance of Pretreatment Peripheral Neutrophil Count and Neutrophil-to-lymphocyte Ratio in Primary Cutaneous Angiosarcoma Open
Systemic inflammatory response markers, including neutrophil-to-lymphocyte ratio, platelet-to-lymphocyte ratio and monocyte-to-lymphocyte ratio, are useful prognostic factors for various malignant tumours. The aim of this study was to inve…
View article: Elongated neutrophil-derived structures are blood-borne microparticles formed by rolling neutrophils during sepsis
Elongated neutrophil-derived structures are blood-borne microparticles formed by rolling neutrophils during sepsis Open
Rolling neutrophils form tethers with submicron diameters. Here, we report that these tethers detach, forming elongated neutrophil-derived structures (ENDS) in the vessel lumen. We studied ENDS formation in mice and humans in vitro and in …
View article: Combined protein and transcript single cell RNA sequencing in human peripheral blood mononuclear cells
Combined protein and transcript single cell RNA sequencing in human peripheral blood mononuclear cells Open
Cryopreserved peripheral blood mononuclear cells (PBMCs) are frequently collected and provide disease- and treatment-relevant data in clinical studies. Here, we developed combined protein (40 antibodies) and transcript single cell (sc)RNA …
View article: Altered Properties of Endothelial Cells and Mesenchymal Stem Cells Underlying the Development of Scleroderma‐like Vasculopathy in KLF5<sup>+/−</sup>;Fli‐1<sup>+/−</sup> Mice
Altered Properties of Endothelial Cells and Mesenchymal Stem Cells Underlying the Development of Scleroderma‐like Vasculopathy in KLF5<sup>+/−</sup>;Fli‐1<sup>+/−</sup> Mice Open
Objective In prevous studies, we established a new animal model, KLF5 +/− ;Fli‐1 +/− mice, in which fundamental pathologic features of systemic sclerosis (SSc) are broadly recapitulated. SSc vasculopathy is believed to occur as a result of…
View article: Fli1 deficiency suppresses RALDH1 activity of dermal dendritic cells and related induction of regulatory T cells: a possible role in scleroderma
Fli1 deficiency suppresses RALDH1 activity of dermal dendritic cells and related induction of regulatory T cells: a possible role in scleroderma Open
Background Aldehyde dehydrogenase 1 (RALDH1)-producing dermal dendritic cells (DCs), a conventional DC subset regulating skin fibrosis, are decreased in the involved skin of patients with systemic sclerosis (SSc). In this study, we investi…
View article: Additional file 3: Figure S3. of Fli1-haploinsufficient dermal fibroblasts promote skin-localized transdifferentiation of Th2-like regulatory T cells
Additional file 3: Figure S3. of Fli1-haploinsufficient dermal fibroblasts promote skin-localized transdifferentiation of Th2-like regulatory T cells Open
Original films of immunoblotting with molecular weight markers. Original films of Figs. 2e and 4a. Molecular weight markers are shown. (PDF 573 kb)
View article: Quiz Correct answer to the quiz. Check your diagnosis A tick bite-induced skin lesion
Quiz Correct answer to the quiz. Check your diagnosis A tick bite-induced skin lesion Open
ENWEndNote BIBJabRef, Mendeley RISPapers, Reference Manager, RefWorks, Zotero AMA Miyagawa T, Saigusa R, Sato S. QuizCorrect answer to the quiz. Check your diagnosisA tick bite-induced skin lesion. Polish Journal of Pathology. 2018;69(1):1…
View article: A case of amelanotic malignant melanoma initially considered as primary dermal melanoma
A case of amelanotic malignant melanoma initially considered as primary dermal melanoma Open
77歳，女性。初診の1年前，左肩に紅色腫瘤を自覚し，その後緩徐な増大傾向があった。前医切除時の検体にて，明瞭な核小体を持ち，核分裂像も散見される類円形および紡錘形の大小不同な核を有する細胞が境界明瞭な胞巣を形成し，免疫染色ではHMB－45一部陽性，Melan－A陰性，S100陽性を示した。腫瘍細胞は真皮にのみみられ，他部位に明らかな原発巣はなかったため，primary dermal melanomaと診断した。拡大切除，センチネルリンパ節生検を行い，センチネルリンパ節に転移…