Anurag S. Rathore
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View article: Integrating Ensemble NSGA‐II for Multi‐Objective Process Optimization: Refolding of Proinsulin as a Case Study
Integrating Ensemble NSGA‐II for Multi‐Objective Process Optimization: Refolding of Proinsulin as a Case Study Open
In‐vitro refolding of biotherapeutic inclusion bodies has long been recognized as a bottleneck in protein production in host systems such as Escherichia coli . Low throughput, costly reagents, and offline analysis often plague refolding de…
View article: Echo-DND: a dual noise diffusion model for robust and precise left ventricle segmentation in echocardiography
Echo-DND: a dual noise diffusion model for robust and precise left ventricle segmentation in echocardiography Open
Recent advancements in diffusion probabilistic models (DPMs) have revolutionized image processing, demonstrating significant potential in medical applications. Accurate segmentation of the left ventricle (LV) in echocardiograms is crucial …
View article: Advanced two-dimensional liquid chromatography workflow for enhanced resolution of protein components in Indian Cobra (Naja naja) venom using hydrophobic interaction-reverse phase chromatography coupled with mass spectrometry
Advanced two-dimensional liquid chromatography workflow for enhanced resolution of protein components in Indian Cobra (Naja naja) venom using hydrophobic interaction-reverse phase chromatography coupled with mass spectrometry Open
Snake venom exhibits complexity due to its composition of a diverse array of proteins and peptides, ranging in molecular masses from approximately 5 kDa to 200 kDa. A comprehensive understanding of the venom proteome is imperative for the …
View article: N-Glycosylation modulators for targeted manipulation of glycosylation for monoclonal antibodies
N-Glycosylation modulators for targeted manipulation of glycosylation for monoclonal antibodies Open
Monoclonal antibodies are extensively used as biotherapeutics for treatment of a variety of diseases. Glycosylation of therapeutic antibodies is considered a critical quality attribute as it influences the effector function, circulatory ha…
View article: Offline Coupling of Hydrophobic Interaction Chromatography–Capillary Zone Electrophoresis for Monitoring Charge‐Based Heterogeneity of Recombinant Monoclonal Antibodies
Offline Coupling of Hydrophobic Interaction Chromatography–Capillary Zone Electrophoresis for Monitoring Charge‐Based Heterogeneity of Recombinant Monoclonal Antibodies Open
A holistic understanding of the charge heterogeneity in monoclonal antibodies (mAbs) is paramount for ensuring acceptable product quality. Hence, biotherapeutic manufacturers are expected to thoroughly characterize their products via advan…
View article: Weakly supervised large-scale pancreatic cancer detection using multi-instance learning
Weakly supervised large-scale pancreatic cancer detection using multi-instance learning Open
Introduction Early detection of pancreatic cancer continues to be a challenge due to the difficulty in accurately identifying specific signs or symptoms that might correlate with the onset of pancreatic cancer. Unlike breast or colon or pr…
View article: Multi-attribute monitoring applications in biopharmaceutical analysis
Multi-attribute monitoring applications in biopharmaceutical analysis Open
Since the introduction of Quality by Design for production of biopharmaceuticals, the global biopharma industry has been advancing towards developing highly efficient and sensitive platforms for monitoring product quality attributes. Incor…
View article: A Cyber–Physical Production System for the Integrated Operation and Monitoring of a Continuous Manufacturing Train for the Production of Monoclonal Antibodies
A Cyber–Physical Production System for the Integrated Operation and Monitoring of a Continuous Manufacturing Train for the Production of Monoclonal Antibodies Open
The continuous manufacturing of biologics offers significant advantages in terms of reducing manufacturing costs and increasing capacity, but it is not yet widely implemented by the industry due to major challenges in the automation, sched…
View article: Challenges in the Analytical Characterization of VLPs Through HPLC-Based Methods
Challenges in the Analytical Characterization of VLPs Through HPLC-Based Methods Open
Characterization and quantification of virus-like particles (VLPs) through high performance liquid chromatography (HPLC)-based methods are challenging because of their large size, structural complexity, internal structural heterogeneity, a…
View article: Impact of Excipient Extraction and Buffer Exchange on Recombinant Monoclonal Antibody Stability
Impact of Excipient Extraction and Buffer Exchange on Recombinant Monoclonal Antibody Stability Open
The foundation of a biosimilar manufacturer's regulatory filing is the demonstration of analytical and functional similarity between the biosimilar product and the pertinent originator product. The excipients in the formulation may interfe…
View article: Reversed phase high performance liquid chromatography for monitoring type-16 human papilloma virus like particles
Reversed phase high performance liquid chromatography for monitoring type-16 human papilloma virus like particles Open
Quantification of virus like particles (VLPs) is challenging due to the large size and structural heterogeneity of the molecule. Structural heterogeneity arises due to random assemblage of L1 protein with other host cell proteins. With mul…
View article: Automated Method for Quantification of 20 Amino Acids in Cell Culture Media During Biopharmaceutical Development
Automated Method for Quantification of 20 Amino Acids in Cell Culture Media During Biopharmaceutical Development Open
Herein, a step-by-step protocol for simultaneous detection of 20 amino acids commonly present in cell culture media is described. The protocol facilitates detection of both primary and secondary amino acids through a two-step precolumn der…
View article: Improved Stability and Manufacturability of Nucleocapsid Antigens for SARS-CoV2 Diagnostics through Protein Engineering
Improved Stability and Manufacturability of Nucleocapsid Antigens for SARS-CoV2 Diagnostics through Protein Engineering Open
The COVID-19 pandemic has had a significant impact on human health management. A rapid diagnosis of SARS-CoV2 at the point-of-care (POC) is critical to prevent disease spread. As a POC device for remote settings, a LFIA should not require …
View article: Does Aggregation of Therapeutic IgGs in PBS Offer a True Picture of What Happens in Models Derived from Human Body Fluids?
Does Aggregation of Therapeutic IgGs in PBS Offer a True Picture of What Happens in Models Derived from Human Body Fluids? Open
Therapeutic proteins such as monoclonal antibodies (mAb) are known to form aggregates due to various factors. Phosphate buffered saline (PBS), human serum, and human serum filtrate (HSF) are some of the models used to analyze mAb stability…
View article: Deep neural network for prediction and control of permeability decline in single pass tangential flow ultrafiltration in continuous processing of monoclonal antibodies
Deep neural network for prediction and control of permeability decline in single pass tangential flow ultrafiltration in continuous processing of monoclonal antibodies Open
Single-pass tangential flow filtration (SPTFF) is a crucial technology enabling the continuous manufacturing of monoclonal antibodies (mAbs). By significantly increasing the membrane area utilized in the process, SPTFF allows the mAb proce…
View article: Monitoring oxidation in recombinant monoclonal antibodies at subunit level through two-dimensional liquid chromatography coupled with mass spectrometry
Monitoring oxidation in recombinant monoclonal antibodies at subunit level through two-dimensional liquid chromatography coupled with mass spectrometry Open
Monoclonal antibodies (mAbs) are highly susceptible to chemical/post-translational modifications (PTMs), a crucial concern for chemical and biological safety during mAb production and development. Modifications in different mAb domains hav…
View article: Profiling Enzyme Activity of <scp>l</scp>-Asparaginase II by NMR-Based Methyl Fingerprinting at Natural Abundance
Profiling Enzyme Activity of <span>l</span>-Asparaginase II by NMR-Based Methyl Fingerprinting at Natural Abundance Open
l-asparaginase II (MW 135 kDa) from E. coli is an FDA-approved protein drug used for the treatment of childhood leukemia. Despite its long history as a chemotherapeutic, the structural basis of enzyme action, in solution, remains widely co…
View article: Abusive Language Detection in Speech Dataset
Abusive Language Detection in Speech Dataset Open
In the topic, speech recognition there is a rapid increasing in it into area of engineering technology. Speech recognition delivers many different kinds of pros and it's been uses in a multiple field. Having a different type of language pl…
View article: Emerging themes and factors influencing the prices of biotherapeutics
Emerging themes and factors influencing the prices of biotherapeutics Open
The high prices of biotherapeutics remain an area of concern for various national and international agencies working toward developing strategies for monitoring, evaluating, and managing the prices of medicines to promote equitable access.…
View article: Data from APOBEC3B Upregulation and Genomic Mutation Patterns in Serous Ovarian Carcinoma
Data from APOBEC3B Upregulation and Genomic Mutation Patterns in Serous Ovarian Carcinoma Open
Ovarian cancer is a clinically and molecularly heterogeneous disease. The driving forces behind this variability are unknown. Here, we report wide variation in the expression of the DNA cytosine deaminase APOBEC3B, with elevated expression…
View article: Supplementary Methods, Tables 1 - 6, Figures 1 - 5 from APOBEC3B Upregulation and Genomic Mutation Patterns in Serous Ovarian Carcinoma
Supplementary Methods, Tables 1 - 6, Figures 1 - 5 from APOBEC3B Upregulation and Genomic Mutation Patterns in Serous Ovarian Carcinoma Open
PDF file - 481K, Table S1: Cell line information Table S2: Quantitative PCR primer and probe sequences. Table S3: Non-malignant tissues tested Table S4: Early stage serous ovarian tumors used in sequence analyses Table S5: Additional ovari…
View article: Data from APOBEC3B Upregulation and Genomic Mutation Patterns in Serous Ovarian Carcinoma
Data from APOBEC3B Upregulation and Genomic Mutation Patterns in Serous Ovarian Carcinoma Open
Ovarian cancer is a clinically and molecularly heterogeneous disease. The driving forces behind this variability are unknown. Here, we report wide variation in the expression of the DNA cytosine deaminase APOBEC3B, with elevated expression…
View article: Supplementary Methods, Tables 1 - 6, Figures 1 - 5 from APOBEC3B Upregulation and Genomic Mutation Patterns in Serous Ovarian Carcinoma
Supplementary Methods, Tables 1 - 6, Figures 1 - 5 from APOBEC3B Upregulation and Genomic Mutation Patterns in Serous Ovarian Carcinoma Open
PDF file - 481K, Table S1: Cell line information Table S2: Quantitative PCR primer and probe sequences. Table S3: Non-malignant tissues tested Table S4: Early stage serous ovarian tumors used in sequence analyses Table S5: Additional ovari…