David S. Boyle
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View article: Urine lipoarabinomannan concentrations among HIV-negative adults with pulmonary or extrapulmonary tuberculosis disease in Vietnam
Urine lipoarabinomannan concentrations among HIV-negative adults with pulmonary or extrapulmonary tuberculosis disease in Vietnam Open
Lipoarabinomannan (LAM) is a promising target biomarker for diagnosing subclinical and clinical tuberculosis (TB). Urine LAM (uLAM) testing using rapid diagnostic tests (RDTs) has been approved for people living with HIV (PLWH), however th…
View article: Development, confirmation, and application of a seeded Escherichia coli process control organism to validate Salmonella enterica serovar Typhi environmental surveillance methods
Development, confirmation, and application of a seeded Escherichia coli process control organism to validate Salmonella enterica serovar Typhi environmental surveillance methods Open
Salmonella enterica serovar Typhi ( S . Typhi) is the causative agent of Typhoid fever. Blood culture is the gold standard for clinical diagnosis, but this is often difficult to employ in resource limited settings. Environmental surveillan…
View article: Cytokine and T cell responses in post-chikungunya viral arthritis: A cross-sectional study
Cytokine and T cell responses in post-chikungunya viral arthritis: A cross-sectional study Open
Objective To define the relationship between chronic chikungunya post-viral arthritis disease severity, cytokine response and T cell subsets in order to identify potential targets for therapy. Methods Participants with chikungunya arthriti…
View article: Correction: Performance of novel antibodies for lipoarabinomannan to develop diagnostic tests for Mycobacterium tuberculosis
Correction: Performance of novel antibodies for lipoarabinomannan to develop diagnostic tests for Mycobacterium tuberculosis Open
[This corrects the article DOI: 10.1371/journal.pone.0274415.].
View article: SARS-CoV-2 recombinase polymerase amplification assay with lateral flow readout and duplexed full process internal control
SARS-CoV-2 recombinase polymerase amplification assay with lateral flow readout and duplexed full process internal control Open
Here we report on a SARS-CoV-2 RPA assay for lateral flow strip detection format that incorporates a full process internal control that indicates the successful lysis, reverse transcription, and amplification of each viral sample.
View article: From biorepositories to data repositories: Open-access resources accelerate early R&D and validation of equitable diagnostic tools
From biorepositories to data repositories: Open-access resources accelerate early R&D and validation of equitable diagnostic tools Open
Diagnostics are critical tools that guide clinical decision-making for patient care and support disease surveillance. Despite its importance, developers and manufacturers often note that access to specimen panels and essential reagents is …
View article: Antigen concentration, viral load, and test performance for SARS-CoV-2 in multiple specimen types
Antigen concentration, viral load, and test performance for SARS-CoV-2 in multiple specimen types Open
The relationship between N-antigen concentration and viral load within and across different specimens guides the clinical performance of rapid diagnostic tests (RDT) in different uses. A prospective study was conducted in Porto Velho, Braz…
View article: Urine lipoarabinomannan concentrations among HIV-uninfected adults with pulmonary or extrapulmonary tuberculosis disease in Vietnam
Urine lipoarabinomannan concentrations among HIV-uninfected adults with pulmonary or extrapulmonary tuberculosis disease in Vietnam Open
Lipoarabinomannan (LAM) is a Mycobacterial cell wall glycolipid excreted in urine, and a target biomarker of rapid diagnostic tests (RDTs) for tuberculosis (TB) disease. Urine LAM (uLAM) testing by RDT has been approved for people living w…
View article: A Reagent and Virus Benchmarking Panel for a Uniform Analytical Performance Assessment of N Antigen-Based Diagnostic Tests for COVID-19
A Reagent and Virus Benchmarking Panel for a Uniform Analytical Performance Assessment of N Antigen-Based Diagnostic Tests for COVID-19 Open
This study reports the results for severe acute respiratory syndrome coronavirus-2 (SARS-COV-2) nucleocapsid (N) and spike (S) antigen quantification assays and their performance against clinical reverse transcription (RT)-PCR results, thu…
View article: 453 Rapid SARS-CoV-2 testing with duplexed recombinase polymerase amplification and a bacteriophage internal control
453 Rapid SARS-CoV-2 testing with duplexed recombinase polymerase amplification and a bacteriophage internal control Open
OBJECTIVES/GOALS: Current COVID-19 rapid molecular tests require cartridge-reader detection, expensive circuitry, and complex microfluidics making the most accurate tests unavailable to the masses. Here we present a rapid molecular diagnos…
View article: Estimates of the cost to build a stand-alone environmental surveillance system for typhoid in low- and middle-income countries
Estimates of the cost to build a stand-alone environmental surveillance system for typhoid in low- and middle-income countries Open
The typhoid conjugate vaccine is a safe and effective method for preventing Salmonella enterica serovar Typhi (typhoid) and the WHO’s guidance supports its use in locations with ongoing transmission. However, many countries lack a robust c…
View article: Multiplexed micronutrient, inflammation, and malarial antigenemia assessment using a plasma fractionation device
Multiplexed micronutrient, inflammation, and malarial antigenemia assessment using a plasma fractionation device Open
Processing and storing blood samples for future analysis of biomarkers can be challenging in resource limited environments. The preparation of dried blood spots (DBS) from finger-stick collection of whole blood is a widely used and establi…
View article: Rapid detection of hepatitis C virus using recombinase polymerase amplification
Rapid detection of hepatitis C virus using recombinase polymerase amplification Open
Over 71 million people are infected with hepatitis C virus (HCV) worldwide, and approximately 400,000 global deaths result from complications of untreated chronic HCV. Pan-genomic direct-acting antivirals (DAAs) have recently become widely…
View article: Performance of novel antibodies for lipoarabinomannan to develop diagnostic tests for Mycobacterium tuberculosis
Performance of novel antibodies for lipoarabinomannan to develop diagnostic tests for Mycobacterium tuberculosis Open
Lipoarabinomannan (LAM), a component of the Mycobacterium tuberculosis (MTB) cell wall, is detectable in the urine of MTB infected patients with active tuberculosis (TB). LAM-specific antibodies (Igs) have been developed by a variety of tr…
View article: Antigen concentration, viral load, and test performance for SARS-CoV-2 in multiple specimen types
Antigen concentration, viral load, and test performance for SARS-CoV-2 in multiple specimen types Open
The relationship between N-antigen concentration and viral load within a specimen and across different specimens is essential for interpretation of rapid diagnostic tests (RDT) clinical performance in different use cases. A prospective stu…
View article: A Reagent and Virus Benchmarking Panel for a Uniform Analytical Performance Assessment of N Antigen–Based Diagnostic Tests for COVID-19
A Reagent and Virus Benchmarking Panel for a Uniform Analytical Performance Assessment of N Antigen–Based Diagnostic Tests for COVID-19 Open
Rapid diagnostic tests (RDTs) that detect antigen indicative of SARS-CoV-2 infection can help in making quick health care decisions and regularly monitoring groups at risk of infection. With many RDT products entering the market, it is imp…
View article: Performance of novel antibodies for lipoarabinomannan to develop diagnostic tests for <i>Mycobacterium tuberculosis</i>
Performance of novel antibodies for lipoarabinomannan to develop diagnostic tests for <i>Mycobacterium tuberculosis</i> Open
Lipoarabinomannan (LAM), a component of the Mycobacterium tuberculosis (MTB) cell wall, is detectable in the urine of MTB infected patients with active tuberculosis (TB). LAM-specific antibodies (Igs) have been developed by a variety of tr…
View article: Performance of novel antibodies for lipoarabinomannan to develop diagnostic tests for Mycobacterium tuberculosis
Performance of novel antibodies for lipoarabinomannan to develop diagnostic tests for Mycobacterium tuberculosis Open
Performance of novel antibodies for 1lipoarabinomannan to develop diagnostic tests for Mycobacterium tuberculosis
View article: Persistence of poliovirus types 2 and 3 in waste-impacted water and sediment
Persistence of poliovirus types 2 and 3 in waste-impacted water and sediment Open
Eradication of poliovirus (PV) is a global public health priority, and as clinical cases decrease, the role of environmental surveillance becomes more important. Persistence of PV and the environmental factors that influence it (such as te…
View article: HIV pre-exposure prophylaxis adherence test using reverse transcription isothermal amplification inhibition assay
HIV pre-exposure prophylaxis adherence test using reverse transcription isothermal amplification inhibition assay Open
We report on a probabilistic isothermal nucleic acid amplification assay for measuring a common medication in HIV prevention and treatment. Expanded access to routine HIV drug adherence testing is a crucial tool in improving the HIV care c…
View article: A SARS-CoV-2 coronavirus nucleocapsid protein antigen-detecting lateral flow assay
A SARS-CoV-2 coronavirus nucleocapsid protein antigen-detecting lateral flow assay Open
Inexpensive, simple, rapid diagnostics are necessary for efficient detection, treatment, and mitigation of COVID-19. Assays for SARS-CoV2 using reverse transcription polymerase chain reaction (RT-PCR) offer good sensitivity and excellent s…
View article: Multiplexed micronutrient, inflammation, and malarial antigenemia assessment using a plasma fractionation device
Multiplexed micronutrient, inflammation, and malarial antigenemia assessment using a plasma fractionation device Open
Collecting, processing, and storing blood samples for future analysis of biomarkers can be challenging when performed in resource limited environments. The preparation of dried blood spots (DBS) from heel or finger stick collection of whol…
View article: A multicenter analytical performance evaluation of a multiplexed immunoarray for the simultaneous measurement of biomarkers of micronutrient deficiency, inflammation and malarial antigenemia
A multicenter analytical performance evaluation of a multiplexed immunoarray for the simultaneous measurement of biomarkers of micronutrient deficiency, inflammation and malarial antigenemia Open
A lack of comparative data across laboratories is often a barrier to the uptake and adoption of new technologies. Furthermore, data generated by different immunoassay methods may be incomparable due to a lack of harmonization. In this mult…
View article: Antibody Screening Results for Anti-Nucleocapsid Antibodies Toward the Development of a Lateral Flow Assay to Detect SARS-CoV-2 Nucleocapsid Protein
Antibody Screening Results for Anti-Nucleocapsid Antibodies Toward the Development of a Lateral Flow Assay to Detect SARS-CoV-2 Nucleocapsid Protein Open
The global COVID-19 pandemic has created an urgent demand for large numbers of inexpensive, accurate, rapid, point-of-care diagnostic tests. Analyte-based assays are suitably rapid and inexpensive and can be rapidly mass-produced, but for …
View article: Screening Antibodies Raised against the Spike Glycoprotein of SARS-CoV-2 to Support the Development of Rapid Antigen Assays
Screening Antibodies Raised against the Spike Glycoprotein of SARS-CoV-2 to Support the Development of Rapid Antigen Assays Open
Severe acute respiratory coronavirus-2 (SARS-CoV-2) is a novel viral pathogen and therefore a challenge to accurately diagnose infection. Asymptomatic cases are common and so it is difficult to accurately identify infected cases to support…