Stefania Stefani
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View article: Rapid detection of Staphylococcus aureus using a fluorescent PoC array for perioperative guidance for infection
Rapid detection of Staphylococcus aureus using a fluorescent PoC array for perioperative guidance for infection Open
Culture and other conventional diagnostic tests are hindered in their practicality as they are time- and labour intensive to perform. Point-of-care (POC) are designed for rapid, on-site pathogen detection and are increasingly recognized as…
View article: Biofilm-Forming ST-45 Staphylococcus aureus Causing Late Pulmonary Homograft Endocarditis Post-Ross Procedure
Biofilm-Forming ST-45 Staphylococcus aureus Causing Late Pulmonary Homograft Endocarditis Post-Ross Procedure Open
Pulmonary homografts are typically resistant to infection and are favored in valve replacement for endocarditis, including in Ross procedures. We report a rare case of late-onset, aggressive pulmonary valve endocarditis occurring seven yea…
View article: The burden of infections caused by Metallo-Beta-LactamaseProducing Enterobacterales in Italy: epidemiology, outcomes, and management
The burden of infections caused by Metallo-Beta-LactamaseProducing Enterobacterales in Italy: epidemiology, outcomes, and management Open
Metallo-β-lactamase (MBL)-producing Enterobacterales represent a growing public health threat due to their intrinsic resistance to several antibiotics. In Italy, the burden of infections caused by these organisms has been steadily increasi…
View article: Active Microbiological Surveillance for Contrasting Multi-Drug-Resistant Pathogens: Comparison Between a Multiplex Real-Time PCR Method and Culture
Active Microbiological Surveillance for Contrasting Multi-Drug-Resistant Pathogens: Comparison Between a Multiplex Real-Time PCR Method and Culture Open
Background/Objectives. Multi-drug-resistant (MDR) microorganisms pose a significant challenge in healthcare settings, particularly with beta-lactam-resistant Gram-negative bacteria and glycopeptide-resistant enterococci. Culture represents…
View article: Evaluating Antimicrobial Susceptibility Testing Methods for Cefiderocol: A Review and Expert Opinion on Current Practices and Future Directions
Evaluating Antimicrobial Susceptibility Testing Methods for Cefiderocol: A Review and Expert Opinion on Current Practices and Future Directions Open
Background: Cefiderocol (FDC) presents challenges in antimicrobial susceptibility testing (AST). The reference standard is the broth microdilution (BMD) method with iron-depleted cation-adjusted Mueller-Hinton broth (ID-CAMHB). Still, it i…
View article: Evaluating Antimicrobial Susceptibility Testing Methods for Cefiderocol: A Review and Expert Opinion on Current Practices and Future Directions
Evaluating Antimicrobial Susceptibility Testing Methods for Cefiderocol: A Review and Expert Opinion on Current Practices and Future Directions Open
Cefiderocol (FDC) presents challenges in antimicrobial susceptibility testing (AST). The broth microdilution (BMD) method with iron-depleted cation-adjusted Mueller-Hinton broth (ID-CAMHB), is the reference standard but cumbersome for rout…
View article: Integrating an LFA Carbapenemase Detection System into the Laboratory Diagnostic Routine: Preliminary Data and Effectiveness Against Enzyme Variants
Integrating an LFA Carbapenemase Detection System into the Laboratory Diagnostic Routine: Preliminary Data and Effectiveness Against Enzyme Variants Open
Background/Objectives. Carbapenemase production is the most diffused carbapenem-resistance mechanism among Enterobacterales, with Klebsiella pneumoniae carbapenemase (KPC), Verona-imipenemase (VIM), New-Delhi metallo-β-lactamase (NDM), imi…
View article: CovidTGI: A tool to investigate the temporal genetic instability of SARS-CoV-2 variants
CovidTGI: A tool to investigate the temporal genetic instability of SARS-CoV-2 variants Open
The COVID-19 pandemic has underscored the need for fast and accurate epidemiology, particularly due to the high observed mutation frequency in SARS-CoV-2. This study aims to explore the evolution of SARS-CoV-2 through a global analysis. To…
View article: BIBLE INNERANCY FOR GEN ALFA: BIBLE IS NOT TOO FAR ECHO (Inneransi Alkitab untuk Gen Alfa: Alkitab Bukanlah Gaung yang Jauh)
BIBLE INNERANCY FOR GEN ALFA: BIBLE IS NOT TOO FAR ECHO (Inneransi Alkitab untuk Gen Alfa: Alkitab Bukanlah Gaung yang Jauh) Open
Menurut penelitian Bible Society, 60% anak Gen Alfa usia 6-12 tahun tidak pernah membaca, mendengar, atau melihat kisah tentang penciptaan ataupun Tuhan dan mukjizat-Nya. Ada 45% orang tua tidak pernah membacakan Firman Tuhan kepada anak-a…
View article: Treatment of sinusitis in children: an Italian intersociety consensus (SIPPS-SIP-SITIP-FIMP-SIAIP-SIMRI-SIM-FIMMG)
Treatment of sinusitis in children: an Italian intersociety consensus (SIPPS-SIP-SITIP-FIMP-SIAIP-SIMRI-SIM-FIMMG) Open
Sinusitis is an inflammation of the mucous membrane of the paranasal sinuses. Bacterial sinusitis usually occurs as a complication of viral infections of the upper respiratory tract and is a frequent cause of medical consultation. The clin…
View article: Rare or Unusual Non-Fermenting Gram-Negative Bacteria: Therapeutic Approach and Antibiotic Treatment Options
Rare or Unusual Non-Fermenting Gram-Negative Bacteria: Therapeutic Approach and Antibiotic Treatment Options Open
Non-fermenting Gram-negative bacteria (NFGNB) are a heterogeneous group of opportunistic pathogens increasingly associated with healthcare-associated infections. While Pseudomonas aeruginosa, Acinetobacter baumannii, and Stenotrophomonas m…
View article: Diagnostic and epidemiological landscape of anaerobic bacteria in Europe, 2020–2023 (ANAEuROBE)
Diagnostic and epidemiological landscape of anaerobic bacteria in Europe, 2020–2023 (ANAEuROBE) Open
View article: Molecular and Clinical Characterization of Invasive Streptococcus pyogenes Isolates: Insights from Two Northern-Italy Centers
Molecular and Clinical Characterization of Invasive Streptococcus pyogenes Isolates: Insights from Two Northern-Italy Centers Open
Streptococcus pyogenes (Group A Streptococcus, GAS) is a Gram-positive pathogen responsible for both superficial and invasive infections (iGAS), with increasing global incidence in recent years. This study aims to characterize the molecula…
View article: Circulation of a Unique Klebsiella pneumoniae Clone, ST147 NDM-1/OXA-48, in Two Diverse Hospitals in Calabria (Italy)
Circulation of a Unique Klebsiella pneumoniae Clone, ST147 NDM-1/OXA-48, in Two Diverse Hospitals in Calabria (Italy) Open
Background/Objectives: Carbapenem-resistant Klebsiella pneumoniae has become endemic in Europe, including in Italy, where its prevalence has risen dramatically, primarily due to epidemic clones harboring metallo-enzymes. This study aims to…
View article: Sequencing Analysis of Invasive Carbapenem-Resistant Klebsiella pneumoniae Isolates Secondary to Gastrointestinal Colonization
Sequencing Analysis of Invasive Carbapenem-Resistant Klebsiella pneumoniae Isolates Secondary to Gastrointestinal Colonization Open
Klebsiella pneumoniae represent a common invasive infection etiological agent, whose potential carbapenem-resistance and hypermucoviscosity complicate the patient’s management. Infection development often derives from gastrointestinal colo…
View article: A multicenter performance evaluation of cefiderocol MIC results: ComASP in comparison to CLSI broth microdilution
A multicenter performance evaluation of cefiderocol MIC results: ComASP in comparison to CLSI broth microdilution Open
The performance of the Liofilchem Compact Antimicrobial Susceptibility Panel (ComASP) Cefiderocol was evaluated in a multicenter study. Enterobacterales, Acinetobacter baumannii , and Pseudomonas aeruginosa clinical isolates and challenge …
View article: [The CCM Project "Phenotypic and molecular screening methodologies for the detection of coloniza-tions due to carbapenem-resistant Enterobacterales (CRE)"].
[The CCM Project "Phenotypic and molecular screening methodologies for the detection of coloniza-tions due to carbapenem-resistant Enterobacterales (CRE)"]. Open
Carbapenem-resistant Enterobacterales (CREs) are globally considered to be a major threat to public health. National and international guidelines emphasize the importance of routine active surveillance policies to prevent their transmissio…
View article: Cell-free supernatant of Lactobacillus gasseri 1A-TV shows a promising activity to eradicate carbapenem-resistant Klebsiella pneumoniae colonization
Cell-free supernatant of Lactobacillus gasseri 1A-TV shows a promising activity to eradicate carbapenem-resistant Klebsiella pneumoniae colonization Open
Background The use of beneficial bacteria like Lactobacillus spp. is a potential innovative approach to fight antibiotic-resistant pathogens. Klebsiella pneumoniae is one of the most concerning multi drug-resistant (MDR) pathogens, and its…
View article: Treatment of acute pharyngitis in children: an Italian intersociety consensus (SIPPS-SIP-SITIP-FIMP-SIAIP-SIMRI-FIMMG)
Treatment of acute pharyngitis in children: an Italian intersociety consensus (SIPPS-SIP-SITIP-FIMP-SIAIP-SIMRI-FIMMG) Open
View article: Issues with Cefiderocol Testing: Comparing Commercial Methods to Broth Microdilution in Iron-Depleted Medium—Analyses of the Performances, ATU, and Trailing Effect According to EUCAST Initial and Revised Interpretation Criteria
Issues with Cefiderocol Testing: Comparing Commercial Methods to Broth Microdilution in Iron-Depleted Medium—Analyses of the Performances, ATU, and Trailing Effect According to EUCAST Initial and Revised Interpretation Criteria Open
Background: The rise of multi-drug-resistant Gram-negative bacteria necessitates the development of new antimicrobial agents. Cefiderocol shows promising activity by exploiting bacterial iron transport systems to penetrate the outer membra…
View article: Prediction of antimicrobial resistance of Klebsiella pneumoniae from genomic data through machine learning
Prediction of antimicrobial resistance of Klebsiella pneumoniae from genomic data through machine learning Open
Antimicrobials, such as antibiotics or antivirals are medications employed to prevent and treat infectious diseases in humans, animals, and plants. Antimicrobial Resistance occurs when bacteria, viruses, and parasites no longer respond to …
View article: The volatile organic compounds detection in MDR Gram-negatives antimicrobial susceptibility testing: Results from a four-month laboratory experience
The volatile organic compounds detection in MDR Gram-negatives antimicrobial susceptibility testing: Results from a four-month laboratory experience Open
Systemic bacterial infections represent a significant clinical challenge due to the increasing resistance rate towards antimicrobials. An essential key to controlling antimicrobial resistance spread is to administer targeted therapy after …
View article: Molecular diversity in fusidic acid–resistant Methicillin Susceptible <i>Staphylococcus</i> <i>aureus</i>
Molecular diversity in fusidic acid–resistant Methicillin Susceptible <i>Staphylococcus</i> <i>aureus</i> Open
Objectives The recent emergence of fusidic acid (FA)–resistant Staphylococcus aureus has underscored the importance of active surveillance in isolating these strains. The molecular basis of fusidic acid resistance and the carriage of virul…
View article: The Global Burden of Sepsis and Septic Shock
The Global Burden of Sepsis and Septic Shock Open
A dysregulated host response to infection causes organ dysfunction in sepsis and septic shock, two potentially fatal diseases. They continue to be major worldwide health burdens with high rates of morbidity and mortality despite advancemen…
View article: Advancing Pathogen Identification: The Role of Digital PCR in Enhancing Diagnostic Power in Different Settings
Advancing Pathogen Identification: The Role of Digital PCR in Enhancing Diagnostic Power in Different Settings Open
Digital polymerase chain reaction (dPCR) has emerged as a groundbreaking technology in molecular biology and diagnostics, offering exceptional precision and sensitivity in nucleic acid detection and quantification. This review highlights t…
View article: Unveiling the Secrets of Acinetobacter baumannii: Resistance, Current Treatments, and Future Innovations
Unveiling the Secrets of Acinetobacter baumannii: Resistance, Current Treatments, and Future Innovations Open
Acinetobacter baumannii represents a significant concern in nosocomial settings, particularly in critically ill patients who are forced to remain in hospital for extended periods. The challenge of managing and preventing this organism is f…
View article: <i>In vitro</i> activity of cefiderocol against European Enterobacterales, including isolates resistant to meropenem and recentβ-lactam/β-lactamase inhibitor combinations
<i>In vitro</i> activity of cefiderocol against European Enterobacterales, including isolates resistant to meropenem and recentβ-lactam/β-lactamase inhibitor combinations Open
Carbapenem-resistant Enterobacterales represent a major health threat and have few approved therapeutic options. Enterobacterales isolates were collected from hospitalized inpatients from 49 sites in six European countries (1 January–31 De…
View article: Management of intra-abdominal infections: recommendations by the Italian council for the optimization of antimicrobial use
Management of intra-abdominal infections: recommendations by the Italian council for the optimization of antimicrobial use Open
View article: The Impact of Enterococcus spp. in the Immunocompromised Host: A Comprehensive Review
The Impact of Enterococcus spp. in the Immunocompromised Host: A Comprehensive Review Open
The immunocompromised host is usually vulnerable to infectious diseases due to broad-spectrum treatments and immunological dysregulation. The Enterococcus genus consists of normal gut commensals, which acquire a leading role in infective p…
View article: Characterisation of microbial communities and quantification of antibiotic resistance genes in Italian wastewater treatment plants using 16S rRNA sequencing and digital PCR
Characterisation of microbial communities and quantification of antibiotic resistance genes in Italian wastewater treatment plants using 16S rRNA sequencing and digital PCR Open
The spread of antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs) in humans, animals and environment is a growing threat to public health. Wastewater treatment plants (WWTPs) are crucial in mitigating the risk of env…