Thomas S. Edgington
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View article: Dose-Dependent Modulation of Tissue Factor Protein and Procoagulant Activity in Human Monocyte-Derived Macrophages by Oxidized Low Density Lipoprotein
Dose-Dependent Modulation of Tissue Factor Protein and Procoagulant Activity in Human Monocyte-Derived Macrophages by Oxidized Low Density Lipoprotein Open
Low concentrations of oxLDL enhance TF expression in MDMs, whereas higher concentrations attenuate TF expression both at baseline as well as following LPS stimulation. Both TF-PCA and TF protein follow this dose-response pattern that is pr…
View article: Factor Xa Binding to Annexin 2 Mediates Signal Transduction via Protease-Activated Receptor 1
Factor Xa Binding to Annexin 2 Mediates Signal Transduction via Protease-Activated Receptor 1 Open
The serine protease zymogen factor X is converted to its catalytically active form factor Xa by the binary complex of factor VIIa bound to its cell surface receptor tissue factor (TF) or by the intrinsic Xase complex, which consists of act…
View article: Surfing tissue factor
Surfing tissue factor Open
Surfing tissue factor From the predicted to the discovery and elucidation of unanticipated functions and biology of potential significance -
View article: Targeting Cell-Impermeable Prodrug Activation to Tumor Microenvironment Eradicates Multiple Drug-Resistant Neoplasms
Targeting Cell-Impermeable Prodrug Activation to Tumor Microenvironment Eradicates Multiple Drug-Resistant Neoplasms Open
The tumor microenvironment is notably enriched with a broad spectrum of proteases. The proteolytic specificities of peptide substrates provide modular chemical tools for the rational design of cell-impermeable prodrugs that are specificall…
View article: Regulation of Tissue Factor–Mediated Initiation of the Coagulation Cascade by Cell Surface Grp78
Regulation of Tissue Factor–Mediated Initiation of the Coagulation Cascade by Cell Surface Grp78 Open
Objective— To test the hypothesis that Grp78 negatively regulates cell surface tissue factor (TF) procoagulant activity and whether this is mediated by physical interaction. Methods and Results— Biopanning with phage-displayed peptidyl lib…
View article: A hybrid fibronectin motif protein as an integrin targeting selective tumor vascular thrombogen
A hybrid fibronectin motif protein as an integrin targeting selective tumor vascular thrombogen Open
Targeted thrombotic eradication of solid tumors is a novel therapeutic strategy. The feasibility, efficacy, selectivity, and safety are dependent on multiple variables of protein design, molecular assembly, vascular target, and exclusive r…
View article: Macromolecular Substrate Affinity for the Tissue Factor-Factor VIIa Complex Is Independent of Scissile Bond Docking
Macromolecular Substrate Affinity for the Tissue Factor-Factor VIIa Complex Is Independent of Scissile Bond Docking Open
The upstream coagulation enzymes are homologous trypsin-like serine proteases that typically function in enzyme-cofactor complexes, exemplified by coagulation factor VIIa (VIIa), which is allosterically activated upon binding to its cell s…
View article: More Cellular Signals for Atherogenesis?
More Cellular Signals for Atherogenesis? Open
he potential importance of the nuclear factor B (NF-B) system as a key player in control of transcription of genes for mediators of a variety of inflammatory responses, from those mediated by cytokine pathways to atherogenesis and thrombog…
View article: Lipopolysaccharide Induction of the Tumor Necrosis Factor-α Promoter in Human Monocytic Cells
Lipopolysaccharide Induction of the Tumor Necrosis Factor-α Promoter in Human Monocytic Cells Open
Biosynthesis of tumor necrosis factor-alpha (TNF-alpha) is predominantly by cells of the monocytic lineage. This study examined the role of various cis-acting regulatory elements in the lipopolysaccharide (LPS) induction of the human TNF-a…
View article: Effector Cell Protease Receptor-1 Is a Vascular Receptor for Coagulation Factor Xa
Effector Cell Protease Receptor-1 Is a Vascular Receptor for Coagulation Factor Xa Open
The binding and assembly of the coagulation proteases on the endothelial cell surface are important steps not only in the generation of thrombin and thrombogenesis, but also in vascular cell signaling. Effector cell protease receptor (EPR-…
View article: Tissue Factor residue Asp44 regulates catalytic function of the bound proteinase Factor VIIa
Tissue Factor residue Asp44 regulates catalytic function of the bound proteinase Factor VIIa Open
The coagulation pathways are initiated by the cell-surface receptor Tissue Factor (TF), which binds the serine proteinase coagulation Factor VIIa (VIIa), resulting in enhanced catalytic function, both amidolytic, towards small pseudo-subst…
View article: Recombinant soluble human tissue factor secreted by <i>Saccharomyces cerevisiae</i> and refolded from <i>Escherichia coli</i> inclusion bodies: glycosylation of mutants, activity and physical characterization
Recombinant soluble human tissue factor secreted by <i>Saccharomyces cerevisiae</i> and refolded from <i>Escherichia coli</i> inclusion bodies: glycosylation of mutants, activity and physical characterization Open
Tissue factor (TF) is the cell-surface transmembrane receptor that initiates both the extrinsic and intrinsic blood coagulation cascades. The abilities of TF to associate with Factor VIIa and Factor X in a ternary complex and to enable pro…
View article: Two independent sets of monoclonal antibodies define neoepitopes linked to soluble ligand binding and leukocyte adhesion functions of activated alpha M beta 2.
Two independent sets of monoclonal antibodies define neoepitopes linked to soluble ligand binding and leukocyte adhesion functions of activated alpha M beta 2. Open
The integrin alpha M beta 2 mediates a variety of events, adhesive, phagocytic, and inflammatory. Evidence has suggested that the functional events may be mediated by the "activated" conformational forms of alpha M beta 2 produced by appro…
View article: Key ligand interface residues in tissue factor contribute independently to factor VIIa binding.
Key ligand interface residues in tissue factor contribute independently to factor VIIa binding. Open
Scanning alanine mutagenesis of the cell surface protease receptor tissue factor suggested importance of residues Lys20, Ile22, Asp58, Arg135, and Phe140 for binding of ligand, the serine protease coagulation factor VIIa. Ligand binding by…
View article: Inhibition of endotoxin-induced activation of coagulation and fibrinolysis by pentoxifylline or by a monoclonal anti-tissue factor antibody in chimpanzees.
Inhibition of endotoxin-induced activation of coagulation and fibrinolysis by pentoxifylline or by a monoclonal anti-tissue factor antibody in chimpanzees. Open
Knowledge of the pathogenetic mechanisms responsible for the activation of the coagulation system associated with endotoxemia is important for the development of improved modalities for prevention and treatment. We analyzed the appearance …
View article: The activation of factor X and prothrombin by recombinant factor VIIa in vivo is mediated by tissue factor.
The activation of factor X and prothrombin by recombinant factor VIIa in vivo is mediated by tissue factor. Open
The human coagulation system continuously generates very small quantities of Factor Xa and thrombin. Current evidence suggests that basal level activation of the hemostatic mechanism occurs via Factor VIIa-dependent activation of Factor X,…
View article: Astrocytes are the primary source of tissue factor in the murine central nervous system. A role for astrocytes in cerebral hemostasis.
Astrocytes are the primary source of tissue factor in the murine central nervous system. A role for astrocytes in cerebral hemostasis. Open
Hemostasis in the brain is of paramount importance because bleeding into the neural parenchyma can result in paralysis, coma, and death. Consistent with this sensitivity to hemorrhage, the brain contains large amounts of tissue factor (TF)…
View article: Tissue factor is expressed on monocytes during simulated extracorporeal circulation.
Tissue factor is expressed on monocytes during simulated extracorporeal circulation. Open
Certain forms of extracorporeal circulation exemplified by cardiopulmonary bypass require continuous high-dose anticoagulation to prevent thromboembolic complications. We hypothesized that monocytes may be stimulated to express tissue fact…
View article: Expression of tissue factor by melanoma cells promotes efficient hematogenous metastasis.
Expression of tissue factor by melanoma cells promotes efficient hematogenous metastasis. Open
Metastasis is a multistep process which requires highly adapted interactions of tumor cells with host target organs. Compared with nonmetastatic cells, metastatic human melanoma cells express 1000-fold higher level of tissue factor (TF), t…
View article: Role of the membrane surface in the activation of human coagulation factor X.
Role of the membrane surface in the activation of human coagulation factor X. Open
Coagulation factor X is activated by the extrinsic Xase complex composed of factor VIIa associated with the integral membrane protein tissue factor. The kinetics of human factor X activation was studied following reconstitution of this rea…
View article: Tissue factor residues 157-167 are required for efficient proteolytic activation of factor X and factor VII.
Tissue factor residues 157-167 are required for efficient proteolytic activation of factor X and factor VII. Open
The cell surface receptor tissue factor (TF) initiates coagulation by supporting the proteolytic activation of factors X and IX as well as VII to active serine proteases. Architectural similarity of TF to the cytokine receptor family sugge…