Scott E. Fraser
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Negative-Valence Neurons in the Larval Zebrafish Pallium Open
An organism’s survival depends on the rapid classification of sensory events as either harmful or beneficial. In mammals, this computation is partially performed by neurons in the amygdala that respond to signals with negative or positive …
Prolactin receptor localization and dynamics: Insights from quantitative imaging and mathematical modeling Open
Signal transduction through the prolactin receptor (PRLR) is crucial in pancreatic β-cell pro-liferation, impacting pancreatic homeostasis. PRLR-induced JAK/STAT signaling is dynamic, involving changes in spatial organization of signaling …
Transforming hyperspectral data into insight: the DREAM approach for pathology Open
Quantitative pathology remains limited due to the need for chemical staining and subjective interpretation of tissue features. Autofluorescence imaging offers a label-free alternative; however, high-dimensional excitation-emission datasets…
Transforming Hyperspectral Data into Insight: The DREAM Approach for Pathology Open
Quantitative pathology remains limited due to the need for chemical staining and subjective interpretation of tissue features. Autofluorescence imaging offers a label-free alternative: however, high-dimensional excitation-emission datasets…
The emerging role of glycine receptor α2 subunit defects in neurodevelopmental disorders Open
Rare neurodevelopmental disorders (NDDs) are one of the most significant unmet challenges in healthcare due to their lifelong nature, high management costs, and recurrence within families. This review will focus on newly-emerging genetic f…
Subcellular Compartmentalization of Glucose Mediated Insulin Secretion Open
Regulation of blood glucose levels depends on the property of beta cells to couple glucose sensing with insulin secretion. This is accomplished by the concentration-dependent flux of glucose through glycolysis and oxidative phosphorylation…
Rapid diffused optical imaging for accurate 3D estimation of subcutaneous tissue features Open
Conventional light imaging in living tissues is limited to depths under 100 μm by the significant tissue scattering. Consequently, few commercial imaging devices can image tissue lesions beneath the surface, or measure their invasion depth…
Multiscale Light Field Microscopy Platform for Multi-purpose Dynamic Volumetric Bioimaging Open
Light field microscopy (LFM) has emerged in recent years as a unique solution for fast, scan-free volumetric imaging of dynamic biological samples. This is achieved by using a microlens array in the detection path to record both the latera…
Studying APOE Aggregation in Live Cells and Human Neurons Open
Background Human Apolipoprotein (APOE) has three isoforms, ε2 , ε3 , and ε4 among which ε4 ( APOE4 ) confers the highest risk for late‐onset Alzheimer’s disease (AD). APOE4 is also the most prone to aggregate among APOE isoforms. Current e…
Decoding Insulin Secretory Granule Maturation Using Genetically Encoded pH Sensors Open
Insulin is a peptide hormone secreted from pancreatic beta cells to regulate blood glucose homeostasis. Maturation of active insulin occurs within insulin secretory granules (ISG) by acidification of the lumen and enzymatic cleavage of ins…
Neural Basis of Number Sense in Larval Zebrafish Open
Significance Numerically responsive neurons are detected by 3 days post-fertilization (dpf) in zebrafish Number-selective neurons preferring quantities greater than one increases after 3 dpf Neurons that are highly responsive to specific n…
Insulator-based dielectrophoresis-assisted separation of insulin secretory vesicles Open
Organelle heterogeneity and inter-organelle contacts within a single cell contribute to the limited sensitivity of current organelle separation techniques, thus hindering organelle subpopulation characterization. Here, we use direct curren…
View article: METAPHOR: Metabolic evaluation through phasor-based hyperspectral imaging and organelle recognition for mouse blastocysts and oocytes
METAPHOR: Metabolic evaluation through phasor-based hyperspectral imaging and organelle recognition for mouse blastocysts and oocytes Open
Only 30% of embryos from in vitro fertilized oocytes successfully implant and develop to term, leading to repeated transfer cycles. To reduce time-to-pregnancy and stress for patients, there is a need for a diagnostic tool to better select…
Label-free metabolic optical biomarkers track stem cell fate transition in real time Open
Tracking stem cell fate transition is crucial for understanding their development and optimizing biomanufacturing. Destructive single-cell methods provide a pseudotemporal landscape of stem cell differentiation but cannot monitor stem cell…
More than double the fun with two-photon excitation microscopy Open
For generations researchers have been observing the dynamic processes of life through the lens of a microscope. This has offered tremendous insights into biological phenomena that span multiple orders of time- and length-scales ranging fro…
View article: Far-red chemigenetic biosensors for multi-dimensional and super-resolved kinase activity imaging
Far-red chemigenetic biosensors for multi-dimensional and super-resolved kinase activity imaging Open
Fluorescent biosensors revolutionized biomedical science by enabling the direct measurement of signaling activities in living cells, yet the current technology is limited in resolution and dimensionality. Here, we introduce highly sensitiv…
SHR and SCR coordinate root patterning and growth early in the cell cycle Open
Precise control of cell division is essential for proper patterning and growth during the development of multicellular organisms. Coordination of formative divisions that generate new tissue patterns with proliferative divisions that promo…
Episodic live imaging of cone photoreceptor maturation in GNAT2-EGFP retinal organoids Open
Fluorescent reporter pluripotent stem cell-derived retinal organoids are powerful tools to investigate cell type-specific development and disease phenotypes. When combined with live imaging, they enable direct and repeated observation of c…
Hand2 represses non-cardiac cell fates through chromatin remodeling at <i>cis-</i> regulatory elements Open
Developmental studies have revealed the importance of the transcription factor Hand2 in cardiac development. Hand2 promotes cardiac progenitor differentiation and epithelial maturation, while repressing other tissue types. The mechanisms u…
Entanglement-enhanced dual-comb spectroscopy Open
Dual-comb interferometry harnesses the interference of two laser frequency combs to provide unprecedented capability in spectroscopy applications. In the past decade, the state-of-the-art systems have reached a point where the signal-to-no…
VoDEx: a Python library for time annotation and management of volumetric functional imaging data Open
Summary In functional imaging studies, accurately synchronizing the time course of experimental manipulations and stimulus presentations with resulting imaging data is crucial for analysis. Current software tools lack such functionality, r…
Application of fluorescence lifetime imaging microscopy to monitor glucose metabolism in pancreatic islets in vivo Open
Glucose stimulated insulin secretion is mediated by glucose metabolism via oxidative phosphorylation generating ATP that triggers membrane depolarization and exocytosis of insulin. In stressed beta cells, glucose metabolism is remodeled, w…
View article: O-237 Metabolic classification of embryos and oocytes based on hyperspectral imaging and machine learning
O-237 Metabolic classification of embryos and oocytes based on hyperspectral imaging and machine learning Open
Study question To classify embryos according to their metabolic fingerprint with a robust, non-invasive methodology Summary answer Our Hyperspectral (HS) phasor analysis coupled to artificial intelligence simultaneously detects 6+ metaboli…
Development of Highly Fluorogenic Styrene Probes for Visualizing RNA in Live Cells Open
Styrene dyes are useful imaging probes and fluorescent sensors due to their strong fluorogenic responses to environmental changes or binding macromolecules. Previously, indole-containing styrene dyes have been reported to selectively bind …
VoDEx: a Python library for time annotation and management of volumetric functional imaging data Open
Summary: In functional imaging studies, accurately synchronizing the time course of experimental manipulations and stimulus presentations with resulting imaging data is crucial for analysis. Current software tools lack such functionality, …
Aboriginal community‐controlled art centres: Keeping Elders strong and connected. Articulating an ontologically situated, intergenerational model of care Open
Objective To articulate how Aboriginal community‐controlled art centres support the role of Elders and older people within an ontologically situated, intergenerational model of care. Methods In this paper, we draw on stories (data) generat…