Satoshi Kishigami
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View article: How Early-Life Programming During Embryogenesis Imprints Cellular Memory
How Early-Life Programming During Embryogenesis Imprints Cellular Memory Open
Cellular memory, or epigenetic memory, represents the capacity for cells to retain information beyond the underlying DNA sequence. This heritable characteristic is primarily governed by epigenetic mechanisms which enable cells to maintain …
View article: Requirement of Lysosomal Two-Pore Channels for Normal Fertilization and Artificial Oocyte Activation in Mice
Requirement of Lysosomal Two-Pore Channels for Normal Fertilization and Artificial Oocyte Activation in Mice Open
Background: Lysosomes serve not only in the degradation of cellular components but also as calcium (Ca2+) stores. In this study, we investigated the effects of trans-Ned19, an inhibitor of lysosomal calcium channels known to block two-pore…
View article: Chloroquine mitigates long-term effects of in vitro culture in mouse embryos
Chloroquine mitigates long-term effects of in vitro culture in mouse embryos Open
Background In vitro culture of preimplantation embryos may increase the risk of long-term effects, such as obesity and metabolic diseases later in life in the offspring. While the long-term consequences of low-protein diets during early de…
View article: The Absence of Bovine Serum Albumin (BSA) in Preimplantation Culture Media Impairs Embryonic Development and Induces Metabolic Alterations in Mouse Offspring
The Absence of Bovine Serum Albumin (BSA) in Preimplantation Culture Media Impairs Embryonic Development and Induces Metabolic Alterations in Mouse Offspring Open
Bovine serum albumin (BSA), the most commonly used protein in preimplantation embryo culture media, performs a variety of physiological functions. However, its involvement in long-term effects remains largely unclear. To investigate its ph…
View article: Heterogeneity in Fluorescence-Stained Sperm Membrane Patterns and Their Dynamic Changes Towards Fertilization in Mice
Heterogeneity in Fluorescence-Stained Sperm Membrane Patterns and Their Dynamic Changes Towards Fertilization in Mice Open
Background: Sperm represent a heterogeneous population crucial for male reproductive success. Additionally, sperm undergo dynamic changes during maturation and capacitation. Despite these well-established processes, the complex nature of s…
View article: A machine learning model for predicting fertilization following short‐term insemination using embryo images
A machine learning model for predicting fertilization following short‐term insemination using embryo images Open
Purpose This study established a machine learning model (MLM) trained on embryo images to predict fertilization following short‐term insemination for early rescue ICSI and compared its predictive performance with the embryologist's manual …
View article: Chloroquine inhibits artificial oocyte activation induced by ethanol or Sr<sup>2+</sup> but not by sperm in mice
Chloroquine inhibits artificial oocyte activation induced by ethanol or Sr<sup>2+</sup> but not by sperm in mice Open
Calcium release from the endoplasmic reticulum via sperm-derived phospholipase C zeta is crucial for oocyte activation during fertilization. Chloroquine (CQ) inhibits the increase in cytoplasmic calcium. This study investigated the effects…
View article: Induction of the Lipid Droplet Formation Genes in Steatohepatitis Mice by Embryo/Postnatal Nutrient Environment Is Associated with Histone Acetylation around the Genes
Induction of the Lipid Droplet Formation Genes in Steatohepatitis Mice by Embryo/Postnatal Nutrient Environment Is Associated with Histone Acetylation around the Genes Open
Recently, we have demonstrated that mice, cultured embryos in α-minimum essential medium (αMEM) and subsequent fed a high-fat, high-sugar diet, developed steatohepatitis. In this study, we investigated using these samples whether the expre…
View article: Vibrational Electron Energy Loss Spectroscopy for Zero-Size Labeling of Amino Acids
Vibrational Electron Energy Loss Spectroscopy for Zero-Size Labeling of Amino Acids Open
Vibrational electron energy-loss spectroscopy (vibEELS) measures the bonding environment of molecules with high spatial resolution. Here, we demonstrate that vibEELS can be used to identify site-specific vibrational signatures of selective…
View article: Assessing the combined impact of fatty liver-induced TGF-β1 and LPS-activated macrophages in fibrosis through a novel 3D serial section methodology
Assessing the combined impact of fatty liver-induced TGF-β1 and LPS-activated macrophages in fibrosis through a novel 3D serial section methodology Open
Non-alcoholic steatohepatitis (NASH), caused by fat buildup, can lead to liver inflammation and damage. Elucidation of the spatial distribution of fibrotic tissue in the fatty liver in NASH can be immensely useful to understand its pathoge…
View article: MARCH8 Targets Cytoplasmic Lysine Residues of Various Viral Envelope Glycoproteins
MARCH8 Targets Cytoplasmic Lysine Residues of Various Viral Envelope Glycoproteins Open
A member of the MARCH E3 ubiquitin ligase family, MARCH8, downregulates many different kinds of host transmembrane proteins, resulting in the regulation of cellular homeostasis. On the other hands, MARCH8 acts as an antiviral factor when i…
View article: From lessons on the long‐term effects of the preimplantation environment on later health to a “modified ART‐DOHaD” animal model
From lessons on the long‐term effects of the preimplantation environment on later health to a “modified ART‐DOHaD” animal model Open
Background At its earliest stages, mammalian embryonic development is apparently simple but vulnerable. The environment during the preimplantation period, which only lasts a couple of days, has been implicated in adult health, extending to…
View article: Development of the Diabetic Kidney Disease Mouse Model Culturing Embryos in α-Minimum Essential Medium In Vitro, and Feeding Barley Diet Attenuated the Pathology
Development of the Diabetic Kidney Disease Mouse Model Culturing Embryos in α-Minimum Essential Medium In Vitro, and Feeding Barley Diet Attenuated the Pathology Open
Diabetic kidney disease (DKD) is a critical complication associated with diabetes; however, there are only a few animal models that can be used to explore its pathogenesis. In the present study, we established a mouse model of DKD using a …
View article: Evaluating the long-term effect of space radiation on the reproductive normality of mammalian sperm preserved on the International Space Station
Evaluating the long-term effect of space radiation on the reproductive normality of mammalian sperm preserved on the International Space Station Open
The effect of space radiation on offspring was evaluated using mouse freeze-dried spermatozoa preserved on the Space Station.
View article: MARCH8 targets cytoplasmic lysine residues of various viral envelope glycoproteins
MARCH8 targets cytoplasmic lysine residues of various viral envelope glycoproteins Open
The host transmembrane protein MARCH8 is a RING finger E3 ubiquitin ligase that downregulates various host transmembrane proteins, such as MHC-II. We have recently reported that MARCH8 expression in virus-producing cells impairs viral infe…
View article: Mice derived from <i>in vitro</i> αMEM-cultured preimplantation embryos exhibit postprandial hyperglycemia and higher inflammatory gene expression in peripheral leukocytes
Mice derived from <i>in vitro</i> αMEM-cultured preimplantation embryos exhibit postprandial hyperglycemia and higher inflammatory gene expression in peripheral leukocytes Open
We examined whether peripheral leukocytes of mice derived from in vitro αMEM-cultured embryos and exhibiting type 2 diabetes had higher expression of inflammatory-related genes associated with the development of atherosclerosis. Also, we e…
View article: MARCH8 inhibits viral infection by two different mechanisms
MARCH8 inhibits viral infection by two different mechanisms Open
Membrane-associated RING-CH 8 (MARCH8) inhibits infection with both HIV-1 and vesicular stomatitis virus G-glycoprotein (VSV-G)-pseudotyped viruses by reducing virion incorporation of envelope glycoproteins. The molecular mechanisms by whi…
View article: Author response: MARCH8 inhibits viral infection by two different mechanisms
Author response: MARCH8 inhibits viral infection by two different mechanisms Open
Article Figures and data Abstract Introduction Results and discussion Materials and methods Appendix 1 Data availability References Decision letter Author response Article and author information Metrics Abstract Membrane-associated RING-CH…
View article: Super-rapid quantitation of the production of HIV-1 harboring a luminescent peptide tag
Super-rapid quantitation of the production of HIV-1 harboring a luminescent peptide tag Open
In studies of HIV-1, virus production is normally monitored by either a reverse transcriptase assay or a p24 antigen capture ELISA. However, these assays are costly and time-consuming for routine handling of a large number of HIV-1 samples…
View article: Naturally mutated spike proteins of SARS-CoV-2 variants show differential levels of cell entry
Naturally mutated spike proteins of SARS-CoV-2 variants show differential levels of cell entry Open
The causative agent of the coronavirus disease 2019 (COVID-19) pandemic, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is steadily mutating during continuous transmission among humans. Such mutations can occur in the spike …
View article: Super-rapid quantitation of the production of HIV-1 harboring a luminescent peptide tag
Super-rapid quantitation of the production of HIV-1 harboring a luminescent peptide tag Open
In virological studies using HIV-1 proviral clones, virus production is normally monitored by either an RT assay or a p24 antigen capture ELISA. However, these assays are costly and time-consuming for routine handling of a large number of …
View article: MARCH8 inhibits viral infection by two different mechanisms
MARCH8 inhibits viral infection by two different mechanisms Open
Membrane-associated RING-CH 8 (MARCH8) inhibits infection with both HIV-1 and vesicular stomatitis virus G-glycoprotein (VSV-G)-pseudotyped viruses by reducing virion incorporation of envelope glycoproteins. The molecular mechanisms by whi…
View article: New cellular imaging of oocytes and preimplantation embryos using Lumitein™: Evaluation of oocyte quality and new information on protein dynamics within the perivitelline space during the one-cell oocyte stage in mice
New cellular imaging of oocytes and preimplantation embryos using Lumitein™: Evaluation of oocyte quality and new information on protein dynamics within the perivitelline space during the one-cell oocyte stage in mice Open
The extracellular matrix between the oocyte and zona pellucida (ZP) plays an important role in mammalian fertilization and preserves the specific environment of the perivitelline space (PVS) during the development of a preimplantation embr…
View article: Small molecule modulation of a redox-sensitive stress granule protein dissolves stress granules with beneficial outcomes for familial amyotrophic lateral sclerosis models
Small molecule modulation of a redox-sensitive stress granule protein dissolves stress granules with beneficial outcomes for familial amyotrophic lateral sclerosis models Open
Neurodegeneràve diseases such as amyotrophic lateral sclerosis (ALS) are oten associated with mutàons in proteins that are associated with stress granules. Stress granules are condensates formed by liquid-liquid phase separàon which, when …
View article: Tolerance of the freeze-dried mouse sperm nucleus to temperatures ranging from −196 °C to 150 °C
Tolerance of the freeze-dried mouse sperm nucleus to temperatures ranging from −196 °C to 150 °C Open
It has long been believed that tolerance against extreme environments is possible only for ‘lower’ groups, such as archaea, bacteria or tardigrades, and not for more ‘advanced’ species. Here, we demonstrated that the mammalian sperm nucleu…