S. Ranil Wickramasinghe
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View article: Downstream Process Intensification for AAV Purification by Affinity Chromatography Using Single Pass Tangential Flow Filtration
Downstream Process Intensification for AAV Purification by Affinity Chromatography Using Single Pass Tangential Flow Filtration Open
To enable adeno‐associated viral vectors (AAV) to achieve their maximum potential, next‐generation manufacturing processes must be developed to make gene therapies more affordable and accessible. This study focused on the design of two dif…
View article: Fabrication of Multimodal Anion Exchange Membrane for Chromatographic Purification of Full Adeno‐Associated Viral Capsids
Fabrication of Multimodal Anion Exchange Membrane for Chromatographic Purification of Full Adeno‐Associated Viral Capsids Open
A multi‐modal anion exchange ligand 2‐methyl‐phenylimidazole has been successfully functionalized on highly porous electrospun regenerated cellulose membrane substrate for the separation of full and empty adeno‐associated viral (AAV) capsi…
View article: The effects of membrane structure and filtration conditions on virus filtration
The effects of membrane structure and filtration conditions on virus filtration Open
View article: Harvesting <scp>AAV</scp> by tangential flow filtration using reverse asymmetric membranes
Harvesting <span>AAV</span> by tangential flow filtration using reverse asymmetric membranes Open
Efficient bioreactor clarification for harvesting virus particles is often challenging. Tangential flow filtration is attractive as it can be easily adapted for batch and perfusion operations. Here the feasibility of using reverse asymmetr…
View article: Optimized Electrocoagulation Pre-Treatment for Fouling Reduction During Nanofiltration of Lake Water Containing Microcystin-LR
Optimized Electrocoagulation Pre-Treatment for Fouling Reduction During Nanofiltration of Lake Water Containing Microcystin-LR Open
Microcystin-LR (MCLR) is a toxin produced by harmful algal blooms that is emerging as a threat to drinking and recreational water systems worldwide. Nanofiltration (NF) is an effective technique for purifying contaminated water sources; ho…
View article: Techno‐Economic Analysis of Membrane‐Based Purification Platforms for AAV Vector Production
Techno‐Economic Analysis of Membrane‐Based Purification Platforms for AAV Vector Production Open
Technologies for large‐scale manufacturing of viral vectors for gene therapies, such as tangential flow filtration and membrane chromatography, are under development. In these early stages of process development, techno‐economic analyses a…
View article: Unleashing the Power of Biologics: Exploring the Governance and Regulation of Membrane-Based Virus Purification (MVP) Technologies
Unleashing the Power of Biologics: Exploring the Governance and Regulation of Membrane-Based Virus Purification (MVP) Technologies Open
Background: Biologics is an exciting and growing area of medicine. Within the larger field of biologics, the use of viral vectors and virus-like particles (VLPs) is increasingly common, making it crucial to develop innovative and practical…
View article: Enhancing the Performance of Tangential Flow Microfiltration for Bioreactor Clarification
Enhancing the Performance of Tangential Flow Microfiltration for Bioreactor Clarification Open
Tangential flow microfiltration is easily adapted for batch and continuous bioreactor clarification. The permeate can be introduced directly to the subsequent capture step. However, the commercial use of tangential flow filtration (TFF) is…
View article: Membrane Filtration of Nanoscale Biomaterials: Model System and Membrane Performance Evaluation for AAV2 Viral Vector Clarification and Recovery
Membrane Filtration of Nanoscale Biomaterials: Model System and Membrane Performance Evaluation for AAV2 Viral Vector Clarification and Recovery Open
The growing demand for viral vectors as nanoscale therapeutic agents in gene therapy necessitates efficient and scalable purification methods. This study examined the role of nanoscale biomaterials in optimizing viral vector clarification …
View article: Enhancing Virus Filter Performance Through Pretreatment by Membrane Adsorbers
Enhancing Virus Filter Performance Through Pretreatment by Membrane Adsorbers Open
Virus filtration is used to ensure the high level of virus clearance required in the manufacture of biopharmaceutical products such as monoclonal antibodies. Flux decline during virus filtration can occur due to the formation of reversible…
View article: Modelling Virus Filtration and Virus Reduction by an Advanced Multilayer Model: Retention Distribution and the Effect of Product Aggregates
Modelling Virus Filtration and Virus Reduction by an Advanced Multilayer Model: Retention Distribution and the Effect of Product Aggregates Open
View article: Modeling virus filtration: Materials, applications, and mechanism
Modeling virus filtration: Materials, applications, and mechanism Open
While various methods are employed to ensure the virus safety of finished products, virus filtration (VF) stands out as the preferred method for virus removal and purification of a wide variety of products owing to its capability of separa…
View article: Carbon-Based Nanocomposite Membranes for Membrane Distillation: Progress, Problems and Future Prospects
Carbon-Based Nanocomposite Membranes for Membrane Distillation: Progress, Problems and Future Prospects Open
The development of an ideal membrane for membrane distillation (MD) is of the utmost importance. Enhancing the efficiency of MD by adding nanoparticles to or onto a membrane’s surface has drawn considerable attention from the scientific co…
View article: A multi-tiered mentoring community approach to research experiences for local students from disadvantaged and underrepresented minority backgrounds
A multi-tiered mentoring community approach to research experiences for local students from disadvantaged and underrepresented minority backgrounds Open
Introduction A research and mentoring program was developed to provide local first-generation students, students returning to school after a professional experience, and underrepresented minority students resources and relationships to gui…
View article: Undergraduate Research and Mentoring in Separations
Undergraduate Research and Mentoring in Separations Open
A Research Experiences and Mentoring (REM) program was developed to provide summer research and mentoring opportunities to underrepresented minority community college students.It was the goal of the program to introduce underrepresented mi…
View article: Electrocoagulation as a pretreatment for reverse osmosis for potable water from brackish groundwater
Electrocoagulation as a pretreatment for reverse osmosis for potable water from brackish groundwater Open
Reverse osmosis (RO) is a widely utilized method for water treatment and desalination. Our study examined the application of an electrocoagulation (EC) and RO hybrid system for treating actual brackish groundwater (BGW), focusing on reduci…
View article: Grand challenges in membrane transport, modeling and simulation
Grand challenges in membrane transport, modeling and simulation Open
SPECIALTY GRAND CHALLENGE article Front. Membr. Sci. Technol., 12 January 2024Sec. Membrane Transport, Modeling and Simulation Volume 2 - 2023 | https://doi.org/10.3389/frmst.2023.1357625
View article: Synergistic Effect of Electrocoagulation and Antifouling Nanofiltration Membranes for Microcystin Removal
Synergistic Effect of Electrocoagulation and Antifouling Nanofiltration Membranes for Microcystin Removal Open
View article: Recovery of Ionic Liquid from the Model Solution Mixture Mimicking the Catalytically Hydrolyzed Cellulose Product Utilizing Amberlyst Ion-Exchange Resin
Recovery of Ionic Liquid from the Model Solution Mixture Mimicking the Catalytically Hydrolyzed Cellulose Product Utilizing Amberlyst Ion-Exchange Resin Open
The hydrolysis of cellulose using ionic liquid (IL) has been extensively studied but there is limited understanding of the removal of IL from the biomass hydrolysate. Finding a suitable method for the recovery and reuse of IL is one of the…
View article: Innovative Approaches to Poultry Processing Wastewater Treatment: The Stainless Steel Ultrafiltration Membrane as a Viable Option
Innovative Approaches to Poultry Processing Wastewater Treatment: The Stainless Steel Ultrafiltration Membrane as a Viable Option Open
In pursuit of sustainability, we explored replacing conventional dissolved air floatation (DAF) in poultry processing wastewater (PPW) treatment with a precisely tuned 0.02 µm stainless-steel ultrafiltration (SSUF) membrane. SSUF is a robu…
View article: Predicting virus filter performance using an advanced membrane structural model
Predicting virus filter performance using an advanced membrane structural model Open
Virus filters are essential in bioprocessing for safe therapeutic protein production. Understanding the correlation between virus filter structure and performance is important for efficient process design. By applying a multilayer structur…
View article: Integrated Electrocoagulation, Ultrafiltration, Membrane Distillation, and Crystallization for Treating Produced Water
Integrated Electrocoagulation, Ultrafiltration, Membrane Distillation, and Crystallization for Treating Produced Water Open
Produced water (PW) generated from hydraulic fracturing operations was treated using an integrated electrocoagulation, ultrafiltration, membrane distillation, and crystallization process (EC UF MDC). The aim was to determine the viability …
View article: Modeling virus filtration based on a multilayer membrane morphology and pore size distribution
Modeling virus filtration based on a multilayer membrane morphology and pore size distribution Open
Obtaining adequate viral clearance in the manufacture of biopharmaceuticals is a major challenge. While virus filtration is routinely used for this purpose, there have been few attempts to model virus filter performance. Here, virus filter…
View article: Use of scanning electron microscopy and energy dispersive X‐ray spectroscopy to identify key fouling species during alternating tangential filtration
Use of scanning electron microscopy and energy dispersive X‐ray spectroscopy to identify key fouling species during alternating tangential filtration Open
Alternating tangential flow filtration (ATF) has become one of the primary methods for cell retention and clarification in perfusion bioreactors. However, membrane fouling can cause product sieving losses that limit the performance of thes…
View article: Supported Ionic Liquid Membrane Contactor with Crown Ether Functionalized Polyimide Membrane for High-Efficient Li+/Mg2+ Selective Separation
Supported Ionic Liquid Membrane Contactor with Crown Ether Functionalized Polyimide Membrane for High-Efficient Li+/Mg2+ Selective Separation Open
View article: Predicting Virus Filter Performance Using an Advanced Membrane Structural Mode
Predicting Virus Filter Performance Using an Advanced Membrane Structural Mode Open
View article: Continuous electrocoagulation-membrane distillation unit for treating hydraulic fracturing produced water
Continuous electrocoagulation-membrane distillation unit for treating hydraulic fracturing produced water Open
View article: Electrospun Hydrophobic Interaction Chromatography (HIC) Membranes for Protein Purification
Electrospun Hydrophobic Interaction Chromatography (HIC) Membranes for Protein Purification Open
Responsive membranes for hydrophobic interaction chromatography have been fabricated by functionalizing poly(N-vinylcaprolactam) (PVCL) ligands on the substrate of electrospun regenerated cellulose nanofibers. Both static and dynamic bindi…
View article: Process- and Product-Related Foulants in Virus Filtration
Process- and Product-Related Foulants in Virus Filtration Open
Regulatory authorities place stringent guidelines on the removal of contaminants during the manufacture of biopharmaceutical products. Monoclonal antibodies, Fc-fusion proteins, and other mammalian cell-derived biotherapeutics are heteroge…
View article: Retention and Fouling during Nanoparticle Filtration: Implications for Membrane Purification of Biotherapeutics
Retention and Fouling during Nanoparticle Filtration: Implications for Membrane Purification of Biotherapeutics Open
One major challenge in the development of nanoparticle-based therapeutics, including viral vectors for the delivery of gene therapies, is the development of cost-effective purification technologies. The objective of this study was to exami…