Sriram Krishnaswamy
YOU?
Author Swipe
View article: Doublet decoding of tRNA<sup>Ser3</sup>demonstrates plasticity of ribosomal decoding center
Doublet decoding of tRNA<sup>Ser3</sup>demonstrates plasticity of ribosomal decoding center Open
Frameshifts can be caused by specific combinations of tRNA and mRNA. The wildtype AGC-decoding E. coli tRNA Ser3 GCU was in the 1980s shown to induce -1 ribosomal frameshifting on GCA alanine codons, and proposed to read a two-base codon i…
View article: An RNA aptamer exploits exosite-dependent allostery to achieve specific inhibition of coagulation factor IXa
An RNA aptamer exploits exosite-dependent allostery to achieve specific inhibition of coagulation factor IXa Open
Hemostasis relies on a reaction network of serine proteases and their cofactors to form a blood clot. Coagulation factor IXa (protease) plays an essential role in hemostasis as evident from the bleeding disease associated with its absence.…
View article: Aptameric hirudins as selective and reversible EXosite-ACTive site (EXACT) inhibitors
Aptameric hirudins as selective and reversible EXosite-ACTive site (EXACT) inhibitors Open
View article: Abstract 2008 The Latent State of Protease Domain in Coagulation Factor IXa Discovered by X-Ray Crystallography Provides Exosite-Mediated Allosteric Control of Blood Clotting
Abstract 2008 The Latent State of Protease Domain in Coagulation Factor IXa Discovered by X-Ray Crystallography Provides Exosite-Mediated Allosteric Control of Blood Clotting Open
Background: Serine proteases of blood coagulation are specifically modulated by cofactors to enable timely hemostatic response without inducing thrombosis. Coagulation factor IXa, a protease component of the enzymic complex called 'intrins…
View article: PB0568 X-Ray Structures of Thrombin Bound to Heterobifunctional Aptamer-Drug Conjugates Reveal the Mechanistic Basis for a Novel Tethered Inhibition Strategy
PB0568 X-Ray Structures of Thrombin Bound to Heterobifunctional Aptamer-Drug Conjugates Reveal the Mechanistic Basis for a Novel Tethered Inhibition Strategy Open
View article: HTRS2023.O6B.2 Allosteric regulation of coagulation factor IXa
HTRS2023.O6B.2 Allosteric regulation of coagulation factor IXa Open
View article: S. Paul Bajaj (1944-2022)
S. Paul Bajaj (1944-2022) Open
View article: Individual phosphatidylinositol transfer proteins have distinct functions that do not involve lipid transfer activity
Individual phosphatidylinositol transfer proteins have distinct functions that do not involve lipid transfer activity Open
Platelets use signal transduction pathways facilitated by class I phosphatidylinositol transfer proteins (PITPs). The 2 mammalian class I PITPs, PITPα and PITPβ, are single PITP domain soluble proteins that are encoded by different genes a…
View article: Generation of an anticoagulant aptamer that targets factor V/Va and disrupts the FVa-membrane interaction in normal and COVID-19 patient samples
Generation of an anticoagulant aptamer that targets factor V/Va and disrupts the FVa-membrane interaction in normal and COVID-19 patient samples Open
View article: Illustrated State‐of‐the‐Art Capsules of the ISTH 2021 Congress
Illustrated State‐of‐the‐Art Capsules of the ISTH 2021 Congress Open
View article: Structure of human factor VIIa–soluble tissue factor with calcium, magnesium and rubidium
Structure of human factor VIIa–soluble tissue factor with calcium, magnesium and rubidium Open
Coagulation factor VIIa (FVIIa) consists of a γ-carboxyglutamic acid (GLA) domain, two epidermal growth factor-like (EGF) domains and a protease domain. FVIIa binds three Mg 2+ ions and four Ca 2+ ions in the GLA domain, one Ca 2+ ion in t…
View article: An Anticoagulant RNA Aptamer That Inhibits Proteinase-Cofactor Interactions within Prothrombinase
An Anticoagulant RNA Aptamer That Inhibits Proteinase-Cofactor Interactions within Prothrombinase Open
The interaction of factor Xa with factor Va on membranes to form prothrombinase profoundly increases the rate of the proteolytic conversion of prothrombin to thrombin. We present the characterization of an RNA aptamer (RNA11F7t) selected f…
View article: Insights into the complex association of bovine factor Va with acidic-lipid-containing synthetic membranes
Insights into the complex association of bovine factor Va with acidic-lipid-containing synthetic membranes Open
The mechanism of binding of blood coagulation cofactor factor Va to acidic-lipid-containing membranes has been addressed. Binding isotherms were generated at room temperature using the change in fluorescence anisotropy of pyrene-labeled bo…
View article: Exosite binding drives substrate affinity for the activation of coagulation factor X by the intrinsic Xase complex
Exosite binding drives substrate affinity for the activation of coagulation factor X by the intrinsic Xase complex Open
Factor X activation by the intrinsic Xase complex, composed of factor IXa bound to factor VIIIa on membranes, is essential for the amplified blood coagulation response. The biological significance of this step is evident from bleeding aris…
View article: FcRn augments induction of tissue factor activity by IgG-containing immune complexes
FcRn augments induction of tissue factor activity by IgG-containing immune complexes Open
Thromboembolism complicates disorders caused by immunoglobulin G (IgG)–containing immune complexes (ICs), but the underlying mechanisms are incompletely understood. Prior evidence indicates that induction of tissue factor (TF) on monocytes…
View article: Occlusion of anion-binding exosite 2 in meizothrombin explains its impaired ability to activate factor V
Occlusion of anion-binding exosite 2 in meizothrombin explains its impaired ability to activate factor V Open
View article: Combination of aptamer and drug for reversible anticoagulation in cardiopulmonary bypass
Combination of aptamer and drug for reversible anticoagulation in cardiopulmonary bypass Open
View article: Advances in Clinical and Basic Science of Coagulation: Illustrated abstracts of the 9th Chapel Hill Symposium on Hemostasis
Advances in Clinical and Basic Science of Coagulation: Illustrated abstracts of the 9th Chapel Hill Symposium on Hemostasis Open
View article: Phosphatidylinositol transfer protein-α in platelets is inconsequential for thrombosis yet is utilized for tumor metastasis
Phosphatidylinositol transfer protein-α in platelets is inconsequential for thrombosis yet is utilized for tumor metastasis Open
Platelets are increasingly recognized for their contributions to tumor metastasis. Here, we show that the phosphoinositide signaling modulated by phosphatidylinositol transfer protein type α (PITPα), a protein which shuttles phosphatidylin…
View article: Selective factor VIII activation by the tissue factor–factor VIIa–factor Xa complex
Selective factor VIII activation by the tissue factor–factor VIIa–factor Xa complex Open
Publisher's Note: There is an Inside Blood Commentary on this article in this issue.
View article: The Fragment 1 Region of Prothrombin Facilitates the Favored Binding of Fragment 12 to Zymogen and Enforces Zymogen-like Character in the Proteinase
The Fragment 1 Region of Prothrombin Facilitates the Favored Binding of Fragment 12 to Zymogen and Enforces Zymogen-like Character in the Proteinase Open
Thrombin is produced from the C-terminal half of prothrombin following its proteolytic activation. The N-terminal half, released as the propiece Fragment 12 (F12), is composed of an N-terminal γ-carboxyglutamate domain (Gla) followed by tw…
View article: FVIII-VWF dos-à-dos
FVIII-VWF dos-à-dos Open
In this issue of Blood, back-to-back (dos-à-dos) papers by Chiu et al and Yee et al present complementary findings of structural investigations into the interaction between factor VIII (FVIII) and von Willebrand factor (VWF). The binding o…