Subba Reddy Palli
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View article: Computational Insights into the Coleopteran-Specific StaufenC in dsRNA Processing
Computational Insights into the Coleopteran-Specific StaufenC in dsRNA Processing Open
The effective regulation of gene expression through RNA interference (RNAi) has emerged as a promising strategy for agricultural pest management and crop protection. Notably, Coleopteran species exhibit a high susceptibility to RNAi, makin…
View article: 20E-induced <i>Kr-h1</i> expression facilitates developmental transitions depending on chromosome accessibility of <i>BR-C</i> enhancers
20E-induced <i>Kr-h1</i> expression facilitates developmental transitions depending on chromosome accessibility of <i>BR-C</i> enhancers Open
Transcription factors and histone modification-mediated chromatin accessibility coordinately regulate spatiotemporal expression of genes that control growth and development. It is well documented that juvenile hormone-activated Kr-h1 antag…
View article: Histone and N‐terminal acetyltransferases play important roles in female reproduction and embryogenesis of the red flour beetle <i>Tribolium castaneum</i>
Histone and N‐terminal acetyltransferases play important roles in female reproduction and embryogenesis of the red flour beetle <i>Tribolium castaneum</i> Open
Histone acetyltransferases (HATs) catalyse the addition of acetyl groups to histones and other proteins. In contrast, histone deacetylases remove acetyl groups from core histones, and the activity of these enzymes maintains the acetylation…
View article: Environmental factors affecting <scp>RNAi</scp> efficacy: Temperature but not plant cultivar influences Colorado potato beetle's response to insecticidal <scp>dsRNA</scp>
Environmental factors affecting <span>RNAi</span> efficacy: Temperature but not plant cultivar influences Colorado potato beetle's response to insecticidal <span>dsRNA</span> Open
Environmental RNAi (eRNAi) is a recent innovation in insect pest control, and comprehensive risk assessment is needed to ensure the environmental safety and longevity of this technology. As eRNAi relies on the insect's cellular machinery f…
View article: Chronologically inappropriate morphogenesis (<i>Chinmo</i>) is required for maintenance of larval stages of fall armyworm
Chronologically inappropriate morphogenesis (<i>Chinmo</i>) is required for maintenance of larval stages of fall armyworm Open
Broad complex (Br-C) and eip93F (E93) transcription factors promote insect metamorphosis from larva to pupa and from pupa to adult, respectively. Recently, chronologically inappropriate morphogenesis (Chinmo) has been proposed as a larval …
View article: Temperature, but not plant cultivar, influence the efficacy of insecticidal dsRNA in Colorado potato beetle
Temperature, but not plant cultivar, influence the efficacy of insecticidal dsRNA in Colorado potato beetle Open
Environmental RNAi (eRNAi) is a recent innovation in insect pest control, and comprehensive risk assessment is needed to ensure the environmental safety and longevity of this technology. As eRNAi relies on the insect’s cellular machinery f…
View article: RNA modifications in insects
RNA modifications in insects Open
More than 100 RNA chemical modifications to cellular RNA have been identified. N 6 -methyladenosine (m 6 A) is the most prevalent modification of mRNA. RNA modifications have recently attracted significant attention due to their critical r…
View article: Deciphering cellular heterogeneity in Spodoptera frugiperda midgut cell line through single cell RNA sequencing
Deciphering cellular heterogeneity in Spodoptera frugiperda midgut cell line through single cell RNA sequencing Open
Using the 10x Genomics Chromium single-cell RNA sequencing (scRNA-seq) platform, we discovered unexpected heterogeneity in an established cell line developed from the midgut of the Fall armyworm, Spodoptera frugiperda, a major global pest.…
View article: Recent advances in understanding of the mechanisms of <scp>RNA</scp> interference in insects
Recent advances in understanding of the mechanisms of <span>RNA</span> interference in insects Open
We highlight the recent 5 years of research that contributed to our understanding of the mechanisms of RNA interference (RNAi) in insects. Since its first discovery, RNAi has contributed enormously as a reverse genetic tool for functional …
View article: StaufenC facilitates utilization of the ERAD pathway to transport dsRNA through the endoplasmic reticulum to the cytosol
StaufenC facilitates utilization of the ERAD pathway to transport dsRNA through the endoplasmic reticulum to the cytosol Open
RNA interference (RNAi) is more efficient in coleopteran insects than other insects. StaufenC (StauC), a coleopteran-specific double-stranded RNA (dsRNA)-binding protein, is required for efficient RNAi in coleopterans. We investigated the …
View article: RNAi turns 25:contributions and challenges in insect science
RNAi turns 25:contributions and challenges in insect science Open
Since its discovery in 1998, RNA interference (RNAi), a Nobel prize-winning technology, made significant contributions to advances in biology because of its ability to mediate the knockdown of specific target genes. RNAi applications in me…
View article: Inefficient uptake of small interfering RNAs is responsible for their inability to trigger RNA interference in Colorado potato beetle cells
Inefficient uptake of small interfering RNAs is responsible for their inability to trigger RNA interference in Colorado potato beetle cells Open
There has been limited success in the usage of exogenous small interference RNA (siRNA) or small hairpin RNA (shRNA) to trigger RNA interference (RNAi) in insects. Instead, long double‐stranded RNAs (dsRNA) are used to induce knockdown of …
View article: Characterization of two cDNAs encoding pH-sensitive and zinc-gated chloride channels in the fall armyworm, Spodoptera frugiperda
Characterization of two cDNAs encoding pH-sensitive and zinc-gated chloride channels in the fall armyworm, Spodoptera frugiperda Open
The pH-sensitive chloride channels (pHCls) are unique chloride channels in invertebrates and have important roles in regulation of fluid secretion, food selection and intake. pHCls are widely expressed in insects, but little is known about…
View article: The pleiotropic AMPK–CncC signaling pathway regulates the trade-off between detoxification and reproduction
The pleiotropic AMPK–CncC signaling pathway regulates the trade-off between detoxification and reproduction Open
The association of decreased fecundity with insecticide resistance and the negative sublethal effects of insecticides on insect reproduction indicates the typical trade-off between two highly energy-demanding processes, detoxification and …
View article: Gene expression in Verson’s glands of the fall armyworm suggests their role in molting and immunity
Gene expression in Verson’s glands of the fall armyworm suggests their role in molting and immunity Open
Verson’s glands are segmental pairs of dermal glands attached to the epidermis in lepidopteran larvae. They produce macromolecules during intermolt period and empty them during each molt. Morphological, histochemical, developmental, and pr…
View article: dsRNase1 contribution to dsRNA degradation activity in the Sf9 cells conditioned medium
dsRNase1 contribution to dsRNA degradation activity in the Sf9 cells conditioned medium Open
RNA interference (RNAi) is inefficient in lepidopteran insects, including Spodoptera frugiperda . RNase activity in the lumen and hemocoel is known to contribute to low RNAi efficiency in these insects. Conditioned medium from Sf9 cells de…
View article: Autophagy genes AMBRA1 and ATG8 play key roles in midgut remodeling of the yellow fever mosquito, Aedes aegypti
Autophagy genes AMBRA1 and ATG8 play key roles in midgut remodeling of the yellow fever mosquito, Aedes aegypti Open
The function of two autophagy genes, an activating molecule BECN1 regulated autophagy (AMBRA1) and autophagy-related gene 8 (ATG8) in the midgut remodeling of Aedes aegypti was investigated. Real-time quantitative polymerase chain reaction…