P. S. Gardner
YOU?
Author Swipe
View article: Rapid viral diagnosis of acute respiratory infections: comparison of enzyme-linked immunosorbent assay and the immunofluorescence technique for detection of viral antigens in nasopharyngeal secretions
Rapid viral diagnosis of acute respiratory infections: comparison of enzyme-linked immunosorbent assay and the immunofluorescence technique for detection of viral antigens in nasopharyngeal secretions Open
Nasopharyngeal secretions from adults and children were obtained in Stockholm, Sweden, for routine diagnosis of influenza A virus, influenza B virus, respiratory syncytial (RS) virus, parainfluenza type 3 virus, and adenovirus infections b…
View article: Public Health Laboratory Service IgM antibody capture enzyme linked immunosorbent assay for detecting rubella specific IgM.
Public Health Laboratory Service IgM antibody capture enzyme linked immunosorbent assay for detecting rubella specific IgM. Open
A total of 468 sera were selected for the evaluation of the Public Health Laboratory Service's IgM antibody capture enzyme linked immunosorbent assay kit (MACELISA) for detecting rubella specific IgM. The results obtained were compared wit…
View article: United Kingdom scheme for external quality assessment in virology. Part II. Specimen distribution, performance assessment, and analyses of participants' methods in detection of rubella antibody, hepatitis B markers, general virus serology, virus identification, and electron microscopy.
United Kingdom scheme for external quality assessment in virology. Part II. Specimen distribution, performance assessment, and analyses of participants' methods in detection of rubella antibody, hepatitis B markers, general virus serology, virus identification, and electron microscopy. Open
Methods for the preparation and pre-distribution testing of specimens for external quality assessment in virology have been defined and criteria for allocation of scores for participants' reports on each category of specimen have been esta…
View article: United Kingdom scheme for external quality assessment in virology. Part I. General method of operation.
United Kingdom scheme for external quality assessment in virology. Part I. General method of operation. Open
Developments in the United Kingdom national external quality assessment scheme for virology are described. There are about 198 participants (170 in the UK) who are enrolled for examination of any or all of five categories of specimen (dist…
View article: Study of discrepancies in rubella haemagglutinin titrations and a reappraisal of diluents used in the rubella haemagglutination inhibition technique.
Study of discrepancies in rubella haemagglutinin titrations and a reappraisal of diluents used in the rubella haemagglutination inhibition technique. Open
To elucidate inconsistencies in rubella haemagglutinin assays the components of the assay technique were examined. The results of carefully controlled assays of rubella haemagglutinin antigens from different sources in various plates and d…
View article: Comparison of antibiotic susceptibility results obtained with Adatab and disc methods.
Comparison of antibiotic susceptibility results obtained with Adatab and disc methods. Open
Strains of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, faecal streptococci, Proteus spp, and Klebsiella spp were distributed on two occasions to two groups of laboratories, one using a commercially produced break point…
View article: Detection of toxin production by Corynebacterium diphtheriae: results of a trial organised as part of the United Kingdom National External Microbiological Quality Assessment Scheme.
Detection of toxin production by Corynebacterium diphtheriae: results of a trial organised as part of the United Kingdom National External Microbiological Quality Assessment Scheme. Open
Four strains of Corynebacterium diphtheriae were sent to UK participants in the UK National External Microbiological Quality Assessment Scheme, who were asked to examine the strains for toxin production by in vitro methods. Laboratories ac…
View article: Comparison of results from two antibiotic susceptibility testing trials that formed part of the United Kingdom national external quality assessment scheme.
Comparison of results from two antibiotic susceptibility testing trials that formed part of the United Kingdom national external quality assessment scheme. Open
A susceptibility testing trial that formed part of the United Kingdom national external quality assessment scheme has been described previously. Results from this first trial showed an association between error rates and particular methods…
View article: Report of a joint DMRQC/Organon field trial to detect hepatitis A IgM by ELISA.
Report of a joint DMRQC/Organon field trial to detect hepatitis A IgM by ELISA. Open
The results of a field trial of a joint DMRQC/Organon ELISA kit for the detection of hepatitis A IgM antibody are reported. The participating laboratories were asked to use the kit to test a panel of 360 specimens consisting of duplicate c…
View article: Enzyme-linked immunosorbent assay (ELISA) for the detection of hepatitis Be antigen and antibody: report of a field trial.
Enzyme-linked immunosorbent assay (ELISA) for the detection of hepatitis Be antigen and antibody: report of a field trial. Open
A field trial of an enzyme-linked immunosorbent assay (ELISA) for the detection of the hepatitis Be markers is reported. It is simple to perform, is designed to be read by eye and does not require any expensive apparatus. When compared wit…
View article: An antibiotic susceptibility testing trial organised as part of the United Kingdom national external microbiological quality assessment scheme.
An antibiotic susceptibility testing trial organised as part of the United Kingdom national external microbiological quality assessment scheme. Open
Organisms of known susceptibility to antimicrobial drugs were distributed for sensitivity testing to laboratories participating in the United Kingdom National External Microbiological Quality Assessment Scheme. The results obtained were co…
View article: The United Kingdom national microbiological quality assessment scheme.
The United Kingdom national microbiological quality assessment scheme. Open
A comprehensive microbiological quality assessment scheme for the benefit of all clinical microbiological laboratories in the United Kingdom was established in 1974. The main emphasis of the scheme has been on the supply of simulated clini…
View article: Comparison of a modified microagglutination technique for HBsAg with the standard technique and a new RIA system.
Comparison of a modified microagglutination technique for HBsAg with the standard technique and a new RIA system. Open
A further modification of the standard RPHA technique (Hepatest, Wellcome Reagents) for the detection of HBsAg is described. This modification does not require a centrifugation step which is required by the other modifications that have be…
View article: Rapid Virus Diagnosis. Application of Immunofluorescence
Rapid Virus Diagnosis. Application of Immunofluorescence Open
Journal Article Rapid Virus Diagnosis. Application of Immunofluorescence Get access By P. S. Gardner and J. Mcquillin. 2nd edn, Pp. xiii + 317, illustrated. Butterworths, London, 1980. £29.00. Postgraduate Medical Journal, Volume 57, Issue…
View article: Atopy does not predispose to RSV bronchiolitis or postbronchiolitic wheezing.
Atopy does not predispose to RSV bronchiolitis or postbronchiolitic wheezing. Open
Twenty-six 8-year-old children who had had respiratory syncytial virus (RSV) bronchiolitis in infancy and their paired controls underwent skin and blood tests to assess the role of immunodeficiency and atopy in the pathogenesis of RSV bron…
View article: In vitro cell-dependent lysis of respiratory syncytial virus-infected cells mediated by antibody from local respiratory secretions.
In vitro cell-dependent lysis of respiratory syncytial virus-infected cells mediated by antibody from local respiratory secretions. Open
Respiratory syncytial (RS) virus causes a local infection of the respiratory tract which is frequently severe in infants. We report the development in infected infants of antibodies in respiratory secretions capable of mediating in vitro d…
View article: Breast-feeding and respiratory syncytial virus infection.
Breast-feeding and respiratory syncytial virus infection. Open
The pattern of breast-feeding in 127 infants admitted to hospital with respiratory syncytial virus infection was compared with that in 503 age-matched controls. Thirty per cent of children with infection had been breast-fed compared with 4…
View article: Complement fixing antibody responses to virus infection in children with acute lymphoblastic leukaemia.
Complement fixing antibody responses to virus infection in children with acute lymphoblastic leukaemia. Open
Thirty children with acute lymphoblastic leukaemia (ALL) were studied and had a virus isolated. Only 50% produced a significant rise in complement fixing (CF) antibody titre compared to 100% of normal children. The failure to produce antib…
View article: Virus infections in children with acute lymphoblastic leukaemia.
Virus infections in children with acute lymphoblastic leukaemia. Open
The pattern of virus isolation and illness was studied in 64 children with acute lymphoblastic leukaemia (ALL) during periods of apparent infection and when the children were well. The virus isolation rate of 2.2 viruses per child a year i…
View article: Viruses and acute abdominal pain in childhood.
Viruses and acute abdominal pain in childhood. Open
Children aged at least 4 years admitted to hospital with acute abdominal pain, excluding appendicitis, were investigated for the presence of viruses. Out of 181 children 29 were found with viruses of whom 18 had nonspecific abdominal pain.…
View article: Cell-free and cell-bound antibody in nasal secretions from infants with respiratory syncytial virus infection
Cell-free and cell-bound antibody in nasal secretions from infants with respiratory syncytial virus infection Open
Twenty-two infants under 9 months of age hospitalized with bronchiolitis or pneumonia due to respiratory syncytial virus (RSV) were serially sampled to determine the pattern of secretory antibody response. Using double labeling techniques,…
View article: Respiratory viruses and cot death.
Respiratory viruses and cot death. Open
Respiratory viruses and histological appearances of the lung were studied prospectively in an unselected series of 104 children who died between 1 week and 2 years of age. Thirty-one of the cases were cot deaths. Seven of these showed evid…
View article: Study of 8-year-old children with a history of respiratory syncytial virus bronchiolitis in infancy.
Study of 8-year-old children with a history of respiratory syncytial virus bronchiolitis in infancy. Open
Thirty-five children known to have had respiratory syncytial virus bronchiolitis in infancy were examined at the age of 8 and their respiratory function tested. The results were compared with those in 35 controls matched for age, sex, and …
View article: Diagnosis of human coronavirus infection by immunofluorescence: Method and application to respiratory disease in hospitalized children
Diagnosis of human coronavirus infection by immunofluorescence: Method and application to respiratory disease in hospitalized children Open
Rabbit antisera were prepared against coronavirus strains 229E and OC43 and used successfully to detect viral antigen in epithelial cells shed from the nasopharynx of symptomatic volunteers who had received coronavirus inocula three to fou…
View article: Role of infection in the death of children with acute lymphoblastic leukaemia.
Role of infection in the death of children with acute lymphoblastic leukaemia. Open
Twenty-four consecutive deaths from a total of 70 children receiving treatment for acute lymphoblastic leukaemia (ALL) have been reviewed. An attempt has been made to ascribe the cause of death to either infection, haemorrhage, the leukaem…
View article: Viruses and febrile convulsions.
Viruses and febrile convulsions. Open
In 276 children admitted to hospital with febrile convulsions a wide range of virus types was identified by means of nasopharyngeal secretions and cough/nasal swabs. The overall virus identification rate was 49%. Analysis of age, sex, fami…