Sarah E. Ewald
YOU?
Author Swipe
View article: Spatially divergent metabolic impact of experimental toxoplasmosis: immunological and microbial correlates
Spatially divergent metabolic impact of experimental toxoplasmosis: immunological and microbial correlates Open
Maladaptive host metabolic responses to infection are emerging as major determinants of infectious disease pathogenesis. However, the factors regulating these metabolic changes within tissues remain poorly understood. In this study, we use…
View article: Macrophage peroxisomes guide alveolar regeneration and limit SARS-CoV-2 tissue sequelae
Macrophage peroxisomes guide alveolar regeneration and limit SARS-CoV-2 tissue sequelae Open
Peroxisomes are vital but often overlooked metabolic organelles. We found that excessive interferon signaling remodeled macrophage peroxisomes. This loss of peroxisomes impaired inflammation resolution and lung repair during severe respira…
View article: Spatially-divergent metabolic impact of experimental toxoplasmosis: immunological and microbial correlates
Spatially-divergent metabolic impact of experimental toxoplasmosis: immunological and microbial correlates Open
Maladaptive host metabolic responses to infection are emerging as major determinants of infectious disease pathogenesis. However, the factors regulating these metabolic changes within tissues remain poorly understood. In this study, we use…
View article: Co-transfection of murine NXPE2 and murine glycophorin A confers reactivity with Ter-119
Co-transfection of murine NXPE2 and murine glycophorin A confers reactivity with Ter-119 Open
Not available.
View article: Conjugation of IL‐33 to Microporous Annealed Particle Scaffolds Enhances Type 2‐Like Immune Responses In Vitro and In Vivo
Conjugation of IL‐33 to Microporous Annealed Particle Scaffolds Enhances Type 2‐Like Immune Responses In Vitro and In Vivo Open
The inflammatory foreign body response (FBR) is the main driver of biomaterial implant failure. Current strategies to mitigate the onset of a FBR include modification of the implant surface, release of anti‐inflammatory drugs, and cell‐sca…
View article: iNOS is necessary for GBP-mediated T. gondii clearance in murine macrophages via vacuole nitration and intravacuolar network collapse
iNOS is necessary for GBP-mediated T. gondii clearance in murine macrophages via vacuole nitration and intravacuolar network collapse Open
Toxoplasma gondii is an obligate intracellular parasite of rodents and humans. Interferon-inducible guanylate binding proteins (GBPs) are mediators of T. gondii clearance, however, this mechanism is incomplete. Here, using automated spatia…
View article: The intersection of host <i>in vivo</i> metabolism and immune responses to infection with kinetoplastid and apicomplexan parasites
The intersection of host <i>in vivo</i> metabolism and immune responses to infection with kinetoplastid and apicomplexan parasites Open
SUMMARY Protozoan parasite infection dramatically alters host metabolism, driven by immunological demand and parasite manipulation strategies. Immunometabolic checkpoints are often exploited by kinetoplastid and protozoan parasites to esta…
View article: NXPE2 Is the Target of Ter-119 When Complexed with Gypa in Mice
NXPE2 Is the Target of Ter-119 When Complexed with Gypa in Mice Open
Background: Red blood cells (RBCs) have a complex biology that allows regulation of metabolism, cellular rigidity, ion exchange, pH, and molecular communication with the vasculature. A central regulator of these biologies are macrocomplexe…
View article: How colonization bottlenecks, tissue niches, and transmission strategies shape protozoan infections
How colonization bottlenecks, tissue niches, and transmission strategies shape protozoan infections Open
Protozoan pathogens such as Plasmodium spp., Leishmania spp., Toxoplasma gondii, and Trypanosoma spp. are often associated with high-mortality, acute and chronic diseases of global health concern. For transmission and immune evasion, proto…
View article: Immunoglobulin <scp>G4</scp> in eosinophilic esophagitis: Immune complex formation and correlation with disease activity
Immunoglobulin <span>G4</span> in eosinophilic esophagitis: Immune complex formation and correlation with disease activity Open
Background Recent studies have shown deposition of immunoglobulin G4 (IgG4) and food proteins in the esophageal mucosa of eosinophilic esophagitis (EoE) patients. Our aims were to assess whether co‐localization of IgG4 and major cow's milk…
View article: Inducible nitric oxide synthase (iNOS) is necessary for GBP-mediated<i>T. gondii</i>restriction in murine macrophages via vacuole nitration and intravacuolar network collapse
Inducible nitric oxide synthase (iNOS) is necessary for GBP-mediated<i>T. gondii</i>restriction in murine macrophages via vacuole nitration and intravacuolar network collapse Open
Toxoplasma gondii is an obligate intracellular, protozoan pathogen of rodents and humans. T. gondii’s ability to grow within cells and evade cell-autonomous immunity depends on the integrity of the parasitophorous vacuole (PV). Interferon-…
View article: Reduced mitochondrial calcium uptake in macrophages is a major driver of inflammaging
Reduced mitochondrial calcium uptake in macrophages is a major driver of inflammaging Open
Mitochondrial dysfunction is linked to age-associated inflammation or inflammaging, but underlying mechanisms are not understood. Analyses of 700 human blood transcriptomes revealed clear signs of age-associated low-grade inflammation. Amo…
View article: Microbial Pathogenesis in the Era of Spatial Omics
Microbial Pathogenesis in the Era of Spatial Omics Open
The biology of a cell, whether it is a unicellular organism or part of a multicellular network, is influenced by cell type, temporal changes in cell state, and the cell’s environment. Spatial cues play a critical role in the regulation of …
View article: Tricarboxylic acid (TCA) cycle, sphingolipid, and phosphatidylcholine metabolism are dysregulated in<i>T. gondii</i>infection-induced cachexia
Tricarboxylic acid (TCA) cycle, sphingolipid, and phosphatidylcholine metabolism are dysregulated in<i>T. gondii</i>infection-induced cachexia Open
Summary Cachexia is a life-threatening disease characterized by chronic, inflammatory muscle wasting and systemic metabolic impairment. Despite its high prevalence, there are no efficacious therapies for cachexia. Mice chronically infected…
View article: Automated Spatially Targeted Optical Microproteomics Investigates Inflammatory Lesions <i>In Situ</i>
Automated Spatially Targeted Optical Microproteomics Investigates Inflammatory Lesions <i>In Situ</i> Open
Tissue microenvironment properties like blood flow, extracellular matrix, or proximity to immune-infiltrate are important regulators of cell biology. However, methods to study regional protein expression in the native tissue environment ar…
View article: Sufis Entangled
Sufis Entangled Open
While revisiting the film Sufis Entangled ten years after its release, the three filmmakers engage in questions of production, form, and historical context. The essays that constitute this piece highlight the intricate ways in which cultur…
View article: Myeloid-mediated IL-1R signaling in immuno-responsive Thy-1 negative fibroblasts is critical for pulmonary fibrosis
Myeloid-mediated IL-1R signaling in immuno-responsive Thy-1 negative fibroblasts is critical for pulmonary fibrosis Open
Idiopathic pulmonary fibrosis (IPF) is a fatal disease with poorly defined pathogenic mechanism and no cure. It is characterized by chronic inflammation, myofibroblast accumulation, and aberrant extracellular matrix (ECM) remodeling. Fibro…
View article: Automated Spatially Targeted Optical Micro Proteomics (AutoSTOMP) 2.0 identifies proteins enriched within inflammatory lesions in tissue sections and human clinical biopsies
Automated Spatially Targeted Optical Micro Proteomics (AutoSTOMP) 2.0 identifies proteins enriched within inflammatory lesions in tissue sections and human clinical biopsies Open
Tissue microenvironment properties like blood flow, extracellular matrix or proximity to immune infiltrate are important regulators of cell biology. However, methods to study regional protein expression in context of the native tissue envi…
View article: Phosphorylation of <i>Toxoplasma gondii</i> Secreted Proteins during Acute and Chronic Stages of Infection
Phosphorylation of <i>Toxoplasma gondii</i> Secreted Proteins during Acute and Chronic Stages of Infection Open
Toxoplasma gondii is a common parasite that infects up to one-third of the human population. Initially, the parasite grows rapidly, infecting and destroying cells of the host, but subsequently switches to a slow-growing form and establishe…
View article: Cellular barcoding of protozoan pathogens reveals the within-host population dynamics of<i>Toxoplasma gondii</i>host colonization
Cellular barcoding of protozoan pathogens reveals the within-host population dynamics of<i>Toxoplasma gondii</i>host colonization Open
Molecular barcoding techniques have emerged as powerful tools to understand microbial pathogenesis. However, barcoding strategies have not been extended to protozoan parasites, which have unique genomic structures and virulence strategies …
View article: The molecular biology and immune control of chronic Toxoplasma gondii infection
The molecular biology and immune control of chronic Toxoplasma gondii infection Open
Toxoplasma gondii is an incredibly successful parasite owing in part to its ability to persist within cells for the life of the host. Remarkably, at least 350 host species of T. gondii have been described to date, and it is estimated that …
View article: IL-1R Regulates Disease Tolerance and Cachexia in <i>Toxoplasma gondii</i> Infection
IL-1R Regulates Disease Tolerance and Cachexia in <i>Toxoplasma gondii</i> Infection Open
Toxoplasma gondii is an obligate intracellular parasite that establishes life-long infection in a wide range of hosts, including humans and rodents. To establish a chronic infection, pathogens often exploit the trade-off between resistance…